Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,654 C→A 23.2% M149I (ATG→ATT)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,6540CA23.2% 801.8 / 184.1 317M149I (ATG→ATT) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (124/119);  new base A (30/44);  total (154/163)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTAT  >  CP009273/3237505‑3237801
                                                                                                                                                     |                                                                                                                                                   
    tAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATct                                                                                                                                                 <  1:3834281/150‑1 (MQ=255)
       aGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgct                                                                                                                                              <  1:1801186/150‑1 (MQ=255)
        ggTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgctg                                                                                                                                             >  1:1304721/1‑150 (MQ=255)
        ggTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgctg                                                                                                                                             <  1:789868/150‑1 (MQ=255)
         gTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCtgctgc                                                                                                                                            <  2:1638602/150‑1 (MQ=255)
            gCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatg                                                                                                                                         >  1:300835/1‑150 (MQ=255)
            gCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatg                                                                                                                                         >  1:4698055/1‑150 (MQ=255)
             ccTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCatga                                                                                                                                        >  2:1800547/1‑150 (MQ=255)
                gTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGc                                                                                                                                     >  2:4243063/1‑150 (MQ=255)
                gTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGc                                                                                                                                     <  1:4809059/150‑1 (MQ=255)
                gTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGc                                                                                                                                     <  1:4809146/150‑1 (MQ=255)
                   cGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgc                                                                                                                                  >  2:891789/1‑150 (MQ=255)
                       gTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcgc                                                                                                                              >  2:1932176/1‑150 (MQ=255)
                           aaGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCGCGTTCATCTGCTGCATGATGCCGCGCGCGgag                                                                                                                          <  2:3081427/150‑1 (MQ=255)
                           aaGGATTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCAGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGgag                                                                                                                          <  1:1512717/150‑1 (MQ=255)
                            aGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGgaga                                                                                                                         <  1:3498194/150‑1 (MQ=255)
                              gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                       >  1:4128139/1‑150 (MQ=255)
                              gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                       >  1:4127969/1‑150 (MQ=255)
                              gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                       <  2:2624099/150‑1 (MQ=255)
                              gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                       <  1:1445671/150‑1 (MQ=255)
                               cTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGt                                                                                                                      >  1:335507/1‑150 (MQ=255)
                                      cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                               >  1:5138839/1‑150 (MQ=255)
                                      cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                               <  1:2186885/150‑1 (MQ=255)
                                       ggACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgc                                                                                                              >  1:75296/1‑150 (MQ=255)
                                        gACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                             <  2:4146006/150‑1 (MQ=255)
                                        gACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                             <  1:4570360/150‑1 (MQ=255)
                                         aCGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcgc                                                                                                            >  1:3382381/1‑150 (MQ=255)
                                           gCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCca                                                                                                          >  2:2231082/1‑150 (MQ=255)
                                            ccAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcag                                                                                                         <  2:1489034/150‑1 (MQ=255)
                                            ccAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcag                                                                                                         >  2:1245883/1‑150 (MQ=255)
                                             cAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagc                                                                                                        >  2:2681871/1‑150 (MQ=255)
                                             cAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagc                                                                                                        <  2:2089091/150‑1 (MQ=255)
                                               gCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGAGCGGAGAAGTTATCCTGCGCcagcag                                                                                                      <  2:3602230/150‑1 (MQ=255)
                                               gCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagcag                                                                                                      <  1:1713584/150‑1 (MQ=255)
                                                    gcagcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGt                                                                                                 <  1:1613986/150‑1 (MQ=255)
                                                     cagcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGtt                                                                                                >  2:2938943/1‑150 (MQ=255)
                                                      agcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTa                                                                                               >  2:3569990/1‑150 (MQ=255)
                                                         aCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAt                                                                                            >  1:1391555/1‑150 (MQ=255)
                                                         aCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAt                                                                                            <  2:2332250/150‑1 (MQ=255)
                                                         aCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAt                                                                                            >  2:144993/1‑150 (MQ=255)
                                                              gATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGACGTTATCCTGCGCCAGCAGTTCGTTACATTCGtt                                                                                       >  1:2722694/1‑150 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                      >  2:3751641/1‑150 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                      <  2:4255758/150‑1 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                      >  2:4433449/1‑150 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACCTTCGTTc                                                                                      >  2:4964581/1‑150 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                      >  2:2422499/1‑150 (MQ=255)
                                                               aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                      <  2:615429/150‑1 (MQ=255)
                                                                 ggTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCa                                                                                    >  2:2527830/1‑150 (MQ=255)
                                                                   tGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGa                                                                                  >  2:830810/1‑150 (MQ=255)
                                                                     aaaGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAtt                                                                                <  1:3900678/150‑1 (MQ=255)
                                                                        gagCCGTCTTGGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCa                                                                             <  1:264511/150‑1 (MQ=255)
                                                                              tCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCa                                                                       <  2:3760203/150‑1 (MQ=255)
                                                                              tCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCa                                                                       <  1:1894424/150‑1 (MQ=255)
                                                                                 ttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTGATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGtt                                                                    <  1:3418417/150‑1 (MQ=255)
                                                                                  tGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTg                                                                   <  1:4938112/150‑1 (MQ=255)
                                                                                  tGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTg                                                                   <  1:438470/150‑1 (MQ=255)
                                                                                   ggCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCCTCGGCAGTTGa                                                                  >  2:3248638/1‑150 (MQ=255)
                                                                                     cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                >  1:2408467/1‑150 (MQ=255)
                                                                                     cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                <  2:1695326/150‑1 (MQ=255)
                                                                                      gATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACgg                                                               >  1:3181126/1‑150 (MQ=255)
                                                                                         ctctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTGCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGACGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATGTCAGCGGCAGTTGACGgaga                                                            >  2:1207760/1‑150 (MQ=255)
                                                                                          tctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGaa                                                           <  2:1045693/150‑1 (MQ=255)
                                                                                          tctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGaa                                                           <  1:2548718/150‑1 (MQ=255)
                                                                                            tGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAg                                                         <  2:4633108/150‑1 (MQ=255)
                                                                                            tGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAg                                                         <  2:3501120/150‑1 (MQ=255)
                                                                                              cGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGagcaagca                                                       >  2:1663158/1‑150 (MQ=255)
                                                                                              cGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCa                                                       <  1:1074523/150‑1 (MQ=255)
                                                                                              cGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCa                                                       <  1:1483692/150‑1 (MQ=255)
                                                                                               gTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCaa                                                      <  1:1245883/150‑1 (MQ=255)
                                                                                                 gATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAtt                                                    >  1:2883039/1‑150 (MQ=255)
                                                                                                   tGCTCCAGAGTATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTg                                                  <  2:2775522/150‑1 (MQ=255)
                                                                                                      tCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCa                                               >  2:4902308/1‑150 (MQ=255)
                                                                                                        cAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGt                                             >  1:2097705/1‑150 (MQ=255)
                                                                                                              atcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAcc                                       >  1:3473995/1‑150 (MQ=255)
                                                                                                               tcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCa                                      <  1:4424223/150‑1 (MQ=255)
                                                                                                                 gatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaaa                                    >  1:4725254/1‑150 (MQ=255)
                                                                                                                   tcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacg                                  <  2:4035489/150‑1 (MQ=255)
                                                                                                                       gTCATCGGTGGTGCCGACCATTTTCACGTTCAGCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGCGAAAGCAATGTGCCAGTGATACCAAacggacg                              >  2:4918757/1‑150 (MQ=255)
                                                                                                                         cATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                            <  1:2379795/150‑1 (MQ=255)
                                                                                                                         cATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                            <  1:3427921/150‑1 (MQ=255)
                                                                                                                         cATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                            <  1:2142518/150‑1 (MQ=255)
                                                                                                                         cATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                            <  1:4158685/150‑1 (MQ=255)
                                                                                                                          aTCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATATCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgcg                           >  2:2041473/1‑150 (MQ=255)
                                                                                                                             ggtggtGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGTCGCAg                        >  2:2977873/1‑150 (MQ=255)
                                                                                                                              gtggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGt                       <  2:304590/150‑1 (MQ=255)
                                                                                                                               tggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGtt                      <  2:5031785/150‑1 (MQ=255)
                                                                                                                                ggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTc                     <  1:3700504/150‑1 (MQ=255)
                                                                                                                                ggtgCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTc                     <  1:590342/150‑1 (MQ=255)
                                                                                                                                  tgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGa                   <  1:4415380/150‑1 (MQ=255)
                                                                                                                                   gCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAg                  >  2:109611/1‑150 (MQ=255)
                                                                                                                                    ccGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAgg                 <  1:333086/150‑1 (MQ=255)
                                                                                                                                    ccGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATGTGCCAGTGATACCAAACGGACGGCGCAGTTCGAgg                 <  2:129092/150‑1 (MQ=255)
                                                                                                                                    ccGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAgg                 >  2:4490504/1‑150 (MQ=255)
                                                                                                                                     cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                >  2:522891/1‑150 (MQ=255)
                                                                                                                                     cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                <  1:427640/150‑1 (MQ=255)
                                                                                                                                      gACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg               <  1:3987462/150‑1 (MQ=255)
                                                                                                                                       aCCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGc              >  2:735780/1‑150 (MQ=255)
                                                                                                                                               cACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTg      >  2:466477/1‑150 (MQ=255)
                                                                                                                                                  gTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGta   >  1:3650289/1‑150 (MQ=255)
                                                                                                                                                   ttCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGtat  >  1:1187236/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTAT  >  CP009273/3237505‑3237801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑