BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,239,086	C→A	9.2%	I208I (ATC→ATA)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,239,086	0	C	A	9.2%	1189.9 / 65.5	325	I208I (ATC→ATA)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base C (153/142); new base A (18/12); total (171/154)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 342 total aligned reads displayed.

GGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAAT  >  CP009273/3238937‑3239235

ggTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatc                                                                                                                                                       <  2:4633848/150‑1 (MQ=255)

ggTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatc                                                                                                                                                       <  2:3851971/150‑1 (MQ=255)

 gTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatca                                                                                                                                                      >  2:396329/1‑150 (MQ=255)

     gAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCAtctc                                                                                                                                                  <  1:4292678/150‑1 (MQ=255)

     gAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCAtctc                                                                                                                                                  >  2:3715626/1‑150 (MQ=255)

       aaGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCg                                                                                                                                                <  2:4322353/150‑1 (MQ=255)

       aaGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCg                                                                                                                                                >  1:2084271/1‑150 (MQ=255)

         gCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGt                                                                                                                                              <  1:3411419/150‑1 (MQ=255)

           cAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGc                                                                                                                                            >  1:3251384/1‑150 (MQ=255)

               tCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTg                                                                                                                                        <  2:742370/150‑1 (MQ=255)

                ccGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGa                                                                                                                                       <  2:3544988/150‑1 (MQ=255)

                  gAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGc                                                                                                                                     <  2:1551928/150‑1 (MQ=255)

                     tGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCttt                                                                                                                                  <  1:1295571/150‑1 (MQ=255)

                      ggTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTa                                                                                                                                 >  2:2291708/1‑150 (MQ=255)

                              gCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCg                                                                                                                         >  1:5127916/1‑150 (MQ=255)

                               cGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGa                                                                                                                        >  1:5057353/1‑150 (MQ=255)

                                 aaaGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatg                                                                                                                      <  1:4811924/150‑1 (MQ=255)

                                 aaaGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatg                                                                                                                      <  1:3337453/150‑1 (MQ=255)

                                    gagCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGTCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggca                                                                                                                   <  1:238182/150‑1 (MQ=255)

                                    gagCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggca                                                                                                                   <  1:2833719/150‑1 (MQ=255)

                                       cGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatgg                                                                                                                >  2:454893/1‑150 (MQ=255)

                                       cGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatgg                                                                                                                <  1:3791444/150‑1 (MQ=255)

                                         ttCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCt                                                                                                              <  1:4992956/150‑1 (MQ=255)

                                              tCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAttt                                                                                                         >  1:1883869/1‑150 (MQ=255)

                                               cGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAtttt                                                                                                        <  1:1473631/150‑1 (MQ=255)

                                                gCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTc                                                                                                       >  1:3951066/1‑150 (MQ=255)

                                                gCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTc                                                                                                       <  2:3212928/150‑1 (MQ=255)

                                                 cAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCt                                                                                                      >  2:253689/1‑150 (MQ=255)

                                                 cAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCt                                                                                                      >  1:1653144/1‑150 (MQ=255)

                                                 cAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCt                                                                                                      >  2:4692135/1‑150 (MQ=255)

                                                  aGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCta                                                                                                     >  2:4933209/1‑150 (MQ=255)

                                                    tAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata                                                                                                   <  2:4259875/150‑1 (MQ=255)

                                                     aGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATaa                                                                                                  >  2:780263/1‑150 (MQ=255)

                                                       gCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAAc                                                                                                <  1:3701048/150‑1 (MQ=255)

                                                         tACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACAt                                                                                              >  2:2125220/1‑150 (MQ=255)

                                                         tACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACAt                                                                                              >  2:2737626/1‑150 (MQ=255)

                                                          aCTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATc                                                                                             <  1:4763437/150‑1 (MQ=255)

                                                          aCTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATc                                                                                             <  1:559396/150‑1 (MQ=255)

                                                               aaCGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcgcgc                                                                                        >  1:143161/1‑150 (MQ=255)

                                                                aCGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcgcgcg                                                                                       <  2:2886740/150‑1 (MQ=255)

                                                                   tAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGAcc                                                                                    >  2:4181003/1‑150 (MQ=255)

                                                                   tAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGAcc                                                                                    >  1:4088759/1‑150 (MQ=255)

                                                                   tAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGAcc                                                                                    >  2:2845579/1‑150 (MQ=255)

                                                                    aGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCa                                                                                   <  1:900085/150‑1 (MQ=255)

                                                                    aGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCa                                                                                   <  1:1705256/150‑1 (MQ=255)

                                                                    aGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCa                                                                                   >  1:2727307/1‑150 (MQ=255)

                                                                     ggTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAg                                                                                  >  1:4382677/1‑150 (MQ=255)

                                                                           tcGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCAt                                                                            <  1:112091/150‑1 (MQ=255)

                                                                             gATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCt                                                                          <  2:4702830/150‑1 (MQ=255)

                                                                              aTTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTg                                                                         >  2:3632638/1‑150 (MQ=255)

                                                                              aTTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTg                                                                         <  2:1898532/150‑1 (MQ=255)

                                                                               ttGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGa                                                                        >  2:4206661/1‑150 (MQ=255)

                                                                                tGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGAc                                                                       <  2:4424684/150‑1 (MQ=255)

                                                                                    gCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGAt                                                                   <  2:585488/150‑1 (MQ=255)

                                                                                    gCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGAt                                                                   >  2:536873/1‑150 (MQ=255)

                                                                                    gCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGAt                                                                   <  2:1158923/150‑1 (MQ=255)

                                                                                    gCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGAt                                                                   <  2:1757224/150‑1 (MQ=255)

                                                                                    gCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGAt                                                                   >  1:2136674/1‑150 (MQ=255)

                                                                                       atgatTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATgaa                                                                >  2:1443328/1‑150 (MQ=255)

                                                                                          atTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATgaagaa                                                             >  1:2583550/1‑150 (MQ=255)

                                                                                               gccgccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGa                                                        >  2:1636862/1‑150 (MQ=255)

                                                                                               gccgccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGa                                                        >  1:1035772/1‑150 (MQ=255)

                                                                                                ccgccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGAc                                                       >  2:1268301/1‑150 (MQ=255)

                                                                                                ccgccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGAc                                                       <  1:1246104/150‑1 (MQ=255)

                                                                                                 cgccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACt                                                      <  2:3424436/150‑1 (MQ=255)

                                                                                                  gccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACta                                                     >  2:4451479/1‑150 (MQ=255)

                                                                                                   ccgcTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACtat                                                    <  1:2893772/150‑1 (MQ=255)

                                                                                                      cTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTAtatt                                                 >  1:1786601/1‑150 (MQ=255)

                                                                                                      cTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTAtatt                                                 >  1:328271/1‑150 (MQ=255)

                                                                                                       tggtggTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTAtatta                                                >  1:3097355/1‑150 (MQ=255)

                                                                                                        ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTAtattat                                               <  2:1170889/150‑1 (MQ=255)

                                                                                                           ggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTaa                                            >  1:4387540/1‑150 (MQ=255)

                                                                                                           ggtATGGCCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTaa                                            >  1:2195344/1‑150 (MQ=255)

                                                                                                              aTGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATgg                                         >  1:4495395/1‑150 (MQ=255)

                                                                                                               tGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGt                                        <  1:1169722/150‑1 (MQ=255)

                                                                                                                gggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTc                                       <  1:4221260/150‑1 (MQ=255)

                                                                                                                 ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCa                                      <  2:3694654/150‑1 (MQ=255)

                                                                                                                   cAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAgg                                    <  2:1046591/150‑1 (MQ=255)

                                                                                                                      tCGTAATGCACAGCTGGCAGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAg                                 >  1:873745/1‑150 (MQ=255)

                                                                                                                       cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATATGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGc                                >  2:4842475/1‑150 (MQ=255)

                                                                                                                        gTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGcc                               >  1:991217/1‑150 (MQ=255)

                                                                                                                         tAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGccc                              <  2:2307875/150‑1 (MQ=255)

                                                                                                                          aaTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCa                             <  1:4184703/150‑1 (MQ=255)

                                                                                                                          aaTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCa                             <  1:4653323/150‑1 (MQ=255)

                                                                                                                          aaTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCa                             <  1:1406057/150‑1 (MQ=255)

                                                                                                                            tGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGc                           <  1:396324/150‑1 (MQ=255)

                                                                                                                              cacaGCTGGCAGTTGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGTCTGATTTTCTATAAACATCCGCGAGAACAGATGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCAc                         <  1:3487338/150‑1 (MQ=255)

                                                                                                                              cacaGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTCTTAATGGTCAGGAAGCCCAGCAc                         >  1:1817595/1‑150 (MQ=255)

                                                                                                                                caGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCa                       <  1:3899675/150‑1 (MQ=255)

                                                                                                                                 aGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAg                      >  1:3147673/1‑150 (MQ=255)

                                                                                                                                      gCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAg                 <  2:2473393/150‑1 (MQ=255)

                                                                                                                                      gCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACAGATGAAGAACTCGACTATATTATTAATGGTCAGGAAGCCCAGCACAAGGagag                 >  2:2969801/1‑150 (MQ=255)

                                                                                                                                        aGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCa               <  2:3913767/150‑1 (MQ=255)

                                                                                                                                        aGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCa               >  1:4093343/1‑150 (MQ=255)

                                                                                                                                        aGATGGCATTTATAATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCa               >  1:2007942/1‑150 (MQ=255)

                                                                                                                                          aTGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACg             >  1:747920/1‑150 (MQ=255)

                                                                                                                                            ggCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGc           >  1:632411/1‑150 (MQ=255)

                                                                                                                                             gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGCg          <  2:2005642/150‑1 (MQ=255)

                                                                                                                                               aTTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGCgaa        >  2:4164289/1‑150 (MQ=255)

                                                                                                                                                     catcTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAAt  <  2:5127727/150‑1 (MQ=255)

GGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAAT  >  CP009273/3238937‑3239235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`