Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,245,848 G→T 90.8% L62L (CTG→CTT)  yhaH → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,245,8480GT90.8% 894.8 / 84.3 316L62L (CTG→CTT) yhaHDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (16/13);  new base T (160/126);  total (177/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

Only 100 of 318 total aligned reads displayed.

GTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAAA  >  CP009273/3245700‑3245994
                                                                                                                                                    |                                                                                                                                                  
gTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTgg                                                                                                                                                   <  2:2146764/150‑1 (MQ=255)
  ttCCTGGGTCGTGCGCGGCGCAAAGAGTACTGGAAGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTtgtg                                                                                                                                                 >  2:1698672/1‑150 (MQ=255)
    ccGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtt                                                                                                                                               >  1:4628346/1‑150 (MQ=255)
     cGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttt                                                                                                                                              >  2:4405866/1‑150 (MQ=255)
     cGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttt                                                                                                                                              <  2:1933182/150‑1 (MQ=255)
     cGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGttt                                                                                                                                              <  2:4206873/150‑1 (MQ=255)
      ggggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                             >  2:3469921/1‑150 (MQ=255)
      ggggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                             >  2:3144832/1‑150 (MQ=255)
      ggggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                             >  1:812784/1‑150 (MQ=255)
      ggggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                             <  1:4917934/150‑1 (MQ=255)
            gTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgt                                                                                                                                       <  1:4484678/150‑1 (MQ=255)
             tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                      >  1:544605/1‑150 (MQ=255)
                gcggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtggtg                                                                                                                                   >  2:3550404/1‑150 (MQ=255)
                 cggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGtggtgg                                                                                                                                  >  1:3704612/1‑150 (MQ=255)
                  ggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTggg                                                                                                                                 >  2:3801325/1‑150 (MQ=255)
                   gcgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGc                                                                                                                                <  1:2390396/150‑1 (MQ=255)
                    cgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCg                                                                                                                               >  2:230923/1‑150 (MQ=255)
                     gcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg                                                                                                                              <  2:1223805/150‑1 (MQ=255)
                      cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGt                                                                                                                             >  2:1541246/1‑150 (MQ=255)
                      cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGt                                                                                                                             <  1:3350127/150‑1 (MQ=255)
                      cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt                                                                                                                             >  2:269217/1‑150 (MQ=255)
                       aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                            >  2:4975095/1‑150 (MQ=255)
                       aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                            >  1:3443770/1‑150 (MQ=255)
                       aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGAGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                            >  2:775642/1‑150 (MQ=255)
                        aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                           >  1:5028370/1‑150 (MQ=255)
                         agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                          <  2:1463371/150‑1 (MQ=255)
                           agTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                        >  2:742112/1‑150 (MQ=255)
                            gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                       >  1:951202/1‑150 (MQ=255)
                             tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                                                      <  1:3278463/150‑1 (MQ=255)
                                tGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgc                                                                                                                   >  2:502740/1‑150 (MQ=255)
                                 ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgcc                                                                                                                  >  1:440685/1‑150 (MQ=255)
                                   aTGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCcgccgc                                                                                                                >  1:3182654/1‑150 (MQ=255)
                                         aTTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCAc                                                                                                          >  2:945894/1‑150 (MQ=255)
                                          ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACg                                                                                                         >  1:2345051/1‑150 (MQ=255)
                                           tCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGa                                                                                                        >  1:2011194/1‑150 (MQ=255)
                                              ggTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGacac                                                                                                     >  1:4878384/1‑150 (MQ=255)
                                                 cAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccga                                                                                                  >  2:1184740/1‑150 (MQ=255)
                                                   aCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgacc                                                                                                <  1:269960/150‑1 (MQ=255)
                                                   aCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgacc                                                                                                <  2:591485/150‑1 (MQ=255)
                                                   aCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgacc                                                                                                <  1:2630474/150‑1 (MQ=255)
                                                         tcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCgg                                                                                          >  2:416682/1‑150 (MQ=255)
                                                            ttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgt                                                                                       <  2:1194288/150‑1 (MQ=255)
                                                               cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                    >  1:3846120/1‑150 (MQ=255)
                                                               cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                    <  1:5098230/150‑1 (MQ=255)
                                                               agTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                    >  1:2272218/2‑150 (MQ=255)
                                                                gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                   >  2:4950261/1‑150 (MQ=255)
                                                                gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCGGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                   <  1:3003123/150‑1 (MQ=255)
                                                                 ttCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGtggtgg                                                                                  <  1:473126/150‑1 (MQ=255)
                                                                   cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                                                >  1:432053/1‑150 (MQ=255)
                                                                   cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                                                >  2:2284330/1‑150 (MQ=255)
                                                                     tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCAc                                                                              >  2:3638022/1‑150 (MQ=255)
                                                                     tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCAc                                                                              >  2:1283117/1‑150 (MQ=255)
                                                                      gCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                                             >  1:346062/1‑150 (MQ=255)
                                                                       cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTg                                                                            <  1:1996444/150‑1 (MQ=255)
                                                                        tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                           >  1:1746689/1‑150 (MQ=255)
                                                                        tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                           <  1:4216209/150‑1 (MQ=255)
                                                                         ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                                          >  1:1310791/1‑150 (MQ=255)
                                                                           ggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                        <  1:4150703/150‑1 (MQ=255)
                                                                           ggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                        <  1:211421/150‑1 (MQ=255)
                                                                            gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                                       <  2:3839856/150‑1 (MQ=255)
                                                                              tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCt                                                                     >  2:2383858/1‑150 (MQ=255)
                                                                                ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                   >  2:1013857/1‑150 (MQ=255)
                                                                                 tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTaa                                                                  >  1:1643150/1‑150 (MQ=255)
                                                                                  ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAt                                                                 >  1:2185815/1‑150 (MQ=255)
                                                                                      aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                             >  1:2961437/1‑150 (MQ=255)
                                                                                             tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                      <  1:1000164/150‑1 (MQ=255)
                                                                                             tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                      >  1:351223/1‑150 (MQ=255)
                                                                                             tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                      >  2:1338689/1‑150 (MQ=255)
                                                                                             gaGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                      <  2:3215844/149‑1 (MQ=255)
                                                                                              aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                                                     <  2:4547476/150‑1 (MQ=255)
                                                                                                 cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctg                                                  <  1:1515495/150‑1 (MQ=255)
                                                                                                 cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctg                                                  <  2:4025029/150‑1 (MQ=255)
                                                                                                  tGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                                                 <  1:4830147/150‑1 (MQ=255)
                                                                                                  tGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                                                 <  2:2771410/150‑1 (MQ=255)
                                                                                                      aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCTTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                             >  1:1610175/1‑150 (MQ=255)
                                                                                                      aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                             >  1:137603/1‑150 (MQ=255)
                                                                                                       aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                            >  2:4301803/1‑150 (MQ=255)
                                                                                                        cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                           >  2:3099586/1‑150 (MQ=255)
                                                                                                                tggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCNt                                   <  1:1087110/150‑1 (MQ=255)
                                                                                                                tggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGt                                   <  2:1298905/150‑1 (MQ=255)
                                                                                                                 ggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTc                                  >  1:1126833/1‑150 (MQ=255)
                                                                                                                  gCGAAGTCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt                                 <  2:514968/150‑1 (MQ=255)
                                                                                                                     caGGCATCCTGACGACACTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTa                              <  2:3925247/149‑1 (MQ=255)
                                                                                                                      aGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa                             <  1:2510608/150‑1 (MQ=255)
                                                                                                                        gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                           <  2:2264570/150‑1 (MQ=255)
                                                                                                                        gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                           >  2:3781492/1‑150 (MQ=255)
                                                                                                                        gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                           <  1:1207834/150‑1 (MQ=255)
                                                                                                                        gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                           >  1:1711405/1‑150 (MQ=255)
                                                                                                                         cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                          >  2:1860653/1‑150 (MQ=255)
                                                                                                                          aTCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGc                         <  1:2528321/150‑1 (MQ=255)
                                                                                                                            ccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                       >  1:2894783/1‑150 (MQ=255)
                                                                                                                             cTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                      <  2:4696049/150‑1 (MQ=255)
                                                                                                                             cTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                      >  1:3898390/1‑150 (MQ=255)
                                                                                                                               gacgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGc                    >  1:5048131/1‑150 (MQ=255)
                                                                                                                                 cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                  >  1:279375/1‑150 (MQ=255)
                                                                                                                                  gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCggg                 <  1:3065661/150‑1 (MQ=255)
                                                                                                                                  gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCggg                 >  2:1795910/1‑150 (MQ=255)
                                                                                                                                   acAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGt                <  1:3694909/150‑1 (MQ=255)
                                                                                                                                    cAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa               >  1:1835663/1‑150 (MQ=255)
                                                                                                                                         tACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCa          <  1:3761849/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAAA  >  CP009273/3245700‑3245994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑