Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,415,551 C→A 7.4% P290T (CCT→ACT)  yhdY → putative amino‑acid transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,415,5510CA 7.4% 1173.7 / 31.2 342P290T (CCT→ACT) yhdYputative amino‑acid transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (133/174);  new base A (25/9);  total (159/183)
Fisher's exact test for biased strand distribution p-value = 9.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

TGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTCGCCGCG  >  CP009273/3415402‑3415700
                                                                                                                                                     |                                                                                                                                                     
tGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTc                                                                                                                                                       >  2:4622556/1‑150 (MQ=255)
 gTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTcc                                                                                                                                                      <  1:3889011/150‑1 (MQ=255)
   ttCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTg                                                                                                                                                    <  1:3759931/150‑1 (MQ=255)
      agtcagCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGc                                                                                                                                                 <  1:3430264/150‑1 (MQ=255)
              tatGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAAt                                                                                                                                         <  2:1335672/150‑1 (MQ=255)
               atGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATa                                                                                                                                        >  1:628340/1‑150 (MQ=255)
                 gTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATAcc                                                                                                                                      >  1:4234298/1‑150 (MQ=255)
                 gTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATAcc                                                                                                                                      <  2:3988911/150‑1 (MQ=255)
                  ttGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACca                                                                                                                                     <  2:3058929/150‑1 (MQ=255)
                    gCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACcatc                                                                                                                                   <  1:4835228/150‑1 (MQ=255)
                     cGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACcatca                                                                                                                                  >  1:808655/1‑150 (MQ=255)
                     cGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACcatca                                                                                                                                  <  1:3935536/150‑1 (MQ=255)
                      ggAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTATGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATAAcatcaa                                                                                                                                 >  1:4349664/1‑149 (MQ=255)
                        aaGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCg                                                                                                                               <  2:772835/150‑1 (MQ=255)
                        aaGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACACAGGGGCTGGTTATTCTGCCCCAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAAAACCATCATCg                                                                                                                               >  2:2614083/1‑150 (MQ=255)
                          gTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCa                                                                                                                             <  2:1559939/150‑1 (MQ=255)
                            cGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCAct                                                                                                                           >  2:5091943/1‑150 (MQ=255)
                             gTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCActc                                                                                                                          >  1:4408490/1‑150 (MQ=255)
                              tGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCActct                                                                                                                         >  2:4777961/1‑150 (MQ=255)
                              tGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGGTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCGGGGCTGGTAAATACCATCATCGCActct                                                                                                                         >  2:557044/1‑150 (MQ=255)
                                 gAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCa                                                                                                                      >  2:3944655/1‑150 (MQ=255)
                                 gAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCAACATCGCACTCGTCa                                                                                                                      >  1:3539314/1‑150 (MQ=255)
                                    gTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAg                                                                                                                   <  2:2571581/150‑1 (MQ=255)
                                    gTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGGTGGGTTACTGGAAAACACAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCAACATCGCACTCTTCAAAg                                                                                                                   >  1:1445795/1‑150 (MQ=255)
                                     tGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAAATCAGGGGCTGGTTATTCTGCCACAGGCGTTTAAGCTGGTAATTCCTGGGCTGGTAAATACCAACATCGCACTCTTCAAAGa                                                                                                                  >  1:1862366/1‑150 (MQ=255)
                                      ggATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGGTGGGTTACTGGAAAACTCAGGGGCTGGGTATTCTGCCACAGGCGGTGAAGCTGGTAAATCCTGGGCGGGGAAATACCAACAACGCACACGTCAAAGAt                                                                                                                 >  1:508367/1‑150 (MQ=255)
                                        aTTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATAc                                                                                                               <  2:4346074/150‑1 (MQ=255)
                                          tACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCa                                                                                                             <  1:1437334/150‑1 (MQ=255)
                                          tACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCa                                                                                                             >  2:3641343/1‑150 (MQ=255)
                                           aCAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAg                                                                                                            <  2:1075654/150‑1 (MQ=255)
                                            cAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGc                                                                                                           >  2:3656488/1‑150 (MQ=255)
                                             aGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGcc                                                                                                          >  2:4868850/1‑150 (MQ=255)
                                               gcgcTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTg                                                                                                        >  2:1790928/1‑150 (MQ=255)
                                                 gcTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGt                                                                                                      >  1:1694932/1‑150 (MQ=255)
                                                 gcTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGt                                                                                                      >  2:3235497/1‑150 (MQ=255)
                                                  cTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAAATCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTg                                                                                                     >  1:1357083/1‑150 (MQ=255)
                                                     ccTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATc                                                                                                  <  1:2391226/150‑1 (MQ=255)
                                                      cTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCa                                                                                                 <  2:2322133/150‑1 (MQ=255)
                                                       tAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGGTGGGTTACAGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCGGGGCTGGTAAATACCATCATCGCACGCTTCAAAGATACCCGCCGGGGGATCAt                                                                                                >  1:4007106/1‑150 (MQ=255)
                                                        aaaGGTCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCAtt                                                                                               >  2:3752516/1‑150 (MQ=255)
                                                         aaGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTa                                                                                              <  1:2438616/150‑1 (MQ=255)
                                                          aGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTAt                                                                                             >  1:4719102/1‑150 (MQ=255)
                                                          aGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTAt                                                                                             <  1:4115203/150‑1 (MQ=255)
                                                                atatGCAGCGGCAGAGTCGCTGGCGTTGGGTGACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGgtt                                                                                       <  2:3299469/150‑1 (MQ=255)
                                                                 tatGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCCTTATCGGgttg                                                                                      >  2:2984711/1‑150 (MQ=255)
                                                                 tatGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGgttg                                                                                      <  1:895476/150‑1 (MQ=255)
                                                                   tGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGgttgtt                                                                                    <  2:2246312/150‑1 (MQ=255)
                                                                    gAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTc                                                                                   >  2:1001511/1‑150 (MQ=255)
                                                                    gAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAAATAAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATAACATCATCGCACTCTTAAAAGATAACAGCCTGGTGATCATTATCGGgttgtta                                                                                   >  2:3540339/1‑149 (MQ=255)
                                                                     aaGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCg                                                                                  >  2:318181/1‑150 (MQ=255)
                                                                        cGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATc                                                                               <  2:144961/150‑1 (MQ=255)
                                                                        cGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATc                                                                               >  2:4107652/1‑150 (MQ=255)
                                                                         ggCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCt                                                                              <  2:2658154/150‑1 (MQ=255)
                                                                         ggCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCAGGGCTGGTAAATACCATCATCGCACACTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCt                                                                              >  2:2456583/1‑150 (MQ=255)
                                                                         ggCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCt                                                                              <  1:3664272/150‑1 (MQ=255)
                                                                           cAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCttt                                                                            <  2:4693167/150‑1 (MQ=255)
                                                                              agTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCa                                                                         <  2:15888/150‑1 (MQ=255)
                                                                               gTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAg                                                                        >  2:1917615/1‑150 (MQ=255)
                                                                                   cTGGCGTTGGGTTACTGGAAAACTCAGGGGATGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGc                                                                    >  2:703801/1‑150 (MQ=255)
                                                                                     ggCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGt                                                                  >  2:4789386/1‑150 (MQ=255)
                                                                                     ggCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGt                                                                  >  1:2596158/1‑150 (MQ=255)
                                                                                        gTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTca                                                               >  1:146221/1‑150 (MQ=255)
                                                                                        gTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTca                                                               >  1:1944514/1‑150 (MQ=255)
                                                                                          tGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTcagc                                                             >  1:2648493/1‑150 (MQ=255)
                                                                                          tGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTc                                                                <  1:917060/147‑1 (MQ=255)
                                                                                          tGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTACTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTc                                                                >  2:917060/1‑147 (MQ=255)
                                                                                           gggTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTcagca                                                            <  1:457186/150‑1 (MQ=255)
                                                                                            ggTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTcagcag                                                           >  1:3033698/1‑150 (MQ=255)
                                                                                              ttACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGc                                                         <  2:2133116/150‑1 (MQ=255)
                                                                                                     aaaaCTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGtt                                                  <  2:2807430/150‑1 (MQ=255)
                                                                                                       aaCTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGa                                                >  1:4065937/1‑150 (MQ=255)
                                                                                                        aCTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGAt                                               <  1:329342/150‑1 (MQ=255)
                                                                                                           cAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATccc                                            <  1:754995/150‑1 (MQ=255)
                                                                                                              gggCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGcc                                         <  2:975536/150‑1 (MQ=255)
                                                                                                                 cTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTgg                                      >  1:2713568/1‑150 (MQ=255)
                                                                                                                  tGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGt                                     <  1:3397181/150‑1 (MQ=255)
                                                                                                                  tGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGt                                     >  2:2026619/1‑150 (MQ=255)
                                                                                                                     ttATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTgg                                  <  1:2064747/150‑1 (MQ=255)
                                                                                                                      tATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTggg                                 <  1:1233428/150‑1 (MQ=255)
                                                                                                                       aTTCTGCCACAGGCGTTGACGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGt                                <  2:808655/150‑1 (MQ=255)
                                                                                                                       aTTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGt                                <  2:4971057/150‑1 (MQ=255)
                                                                                                                       aTTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGt                                <  2:2858132/150‑1 (MQ=255)
                                                                                                                       aTTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGt                                <  1:2103614/150‑1 (MQ=255)
                                                                                                                       aTTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGt                                >  1:2274248/1‑150 (MQ=255)
                                                                                                                          cTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATg                             >  2:3406739/1‑150 (MQ=255)
                                                                                                                           tGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGt                            <  1:2866705/150‑1 (MQ=255)
                                                                                                                           tGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGt                            <  2:4484598/150‑1 (MQ=255)
                                                                                                                            gCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTc                           >  1:1897933/1‑150 (MQ=255)
                                                                                                                             ccACAGGCGATGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCg                          <  2:1290722/150‑1 (MQ=255)
                                                                                                                               acaGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGAc                        >  1:4226757/1‑150 (MQ=255)
                                                                                                                                 aGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACgg                      >  2:2548899/1‑150 (MQ=255)
                                                                                                                                   gCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGaa                    <  1:4778134/150‑1 (MQ=255)
                                                                                                                                     gTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAgg                  >  2:3148414/1‑150 (MQ=255)
                                                                                                                                       tGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGt                >  1:2551721/1‑150 (MQ=255)
                                                                                                                                        gAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTa               >  1:1124205/1‑150 (MQ=255)
                                                                                                                                            cTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGtt           >  2:1227529/1‑150 (MQ=255)
                                                                                                                                             tGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGttt          <  1:105960/150‑1 (MQ=255)
                                                                                                                                                tAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTcg       <  1:1832159/150‑1 (MQ=255)
                                                                                                                                                 aaTTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTcgc      >  1:1639009/1‑150 (MQ=255)
                                                                                                                                                  aTTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTcgcc     >  1:2680945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAAACTCAGGGGCTGGTTATTCTGCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTCGCCGCG  >  CP009273/3415402‑3415700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑