Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,832 T→C 89.9% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,8320TC89.9% 1189.2 / 96.1 355N99D (AAC→GAC) rplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base T (21/15);  new base C (160/159);  total (181/174)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 355 total aligned reads displayed.

ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCC  >  CP009273/3440685‑3440977
                                                                                                                                                   |                                                                                                                                                 
aTGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAg                                                                                                                                                 <  2:422107/150‑1 (MQ=255)
  gTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGAc                                                                                                                                               <  2:3898738/150‑1 (MQ=255)
   ttCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACt                                                                                                                                              <  2:2769869/150‑1 (MQ=255)
   ttCAGGGTGATCTTCTCGACCCGAGGGACGTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACt                                                                                                                                              <  1:4503446/150‑1 (MQ=255)
    tCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACtt                                                                                                                                             <  1:952428/150‑1 (MQ=255)
      aGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTga                                                                                                                                           >  1:880037/1‑150 (MQ=255)
         gtgtTCTTCTCGACCCGAGGGACTTTCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaaga                                                                                                                                        <  1:3428984/150‑1 (MQ=255)
          tGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaagaa                                                                                                                                       >  1:2099262/1‑150 (MQ=255)
           gATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGaaa                                                                                                                                      >  1:2627710/1‑150 (MQ=255)
            aTCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAAc                                                                                                                                     >  1:1834143/1‑150 (MQ=255)
             tcttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACg                                                                                                                                    <  2:1548783/150‑1 (MQ=255)
               ttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGa                                                                                                                                  >  2:96248/1‑150 (MQ=255)
               ttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGa                                                                                                                                  <  1:5025051/150‑1 (MQ=255)
                  tcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtt                                                                                                                               <  2:2194268/150‑1 (MQ=255)
                    gACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttt                                                                                                                             >  2:1377264/1‑150 (MQ=255)
                    gACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttt                                                                                                                             >  2:3455903/1‑150 (MQ=255)
                      cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                           >  2:1392312/1‑150 (MQ=255)
                       ccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTa                                                                                                                          >  1:3105893/1‑150 (MQ=255)
                        cGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAc                                                                                                                         <  2:1271353/150‑1 (MQ=255)
                          aGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCg                                                                                                                       >  2:4793058/1‑150 (MQ=255)
                          aGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCg                                                                                                                       >  2:860730/1‑150 (MQ=255)
                           gggACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGt                                                                                                                      <  1:5002459/150‑1 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTGTTTTACCGTCt                                                                                                                    >  2:4533695/1‑150 (MQ=255)
                              aCTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCtt                                                                                                                   <  1:288096/150‑1 (MQ=255)
                               cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCACATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                                                                                  <  2:200533/150‑1 (MQ=255)
                                ttGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCg                                                                                                                 <  1:1262853/150‑1 (MQ=255)
                                ttGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCg                                                                                                                 <  2:270180/150‑1 (MQ=255)
                                ttGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCg                                                                                                                 >  1:3758838/1‑150 (MQ=255)
                                 tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAGTTTTTTACCGTCTTCGa                                                                                                                >  2:1445944/1‑150 (MQ=255)
                                 tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAACGTCTTAGa                                                                                                                >  1:1974089/1‑150 (MQ=255)
                                   cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                                                                              <  1:1496477/150‑1 (MQ=255)
                                   cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                                                                              <  2:4349057/150‑1 (MQ=255)
                                      gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTa                                                                                                           >  1:4159036/1‑150 (MQ=255)
                                        cAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                         <  2:177072/150‑1 (MQ=255)
                                        cAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                         >  2:4645962/1‑150 (MQ=255)
                                         aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                        >  2:1069611/1‑150 (MQ=255)
                                          gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGc                                                                                                       <  2:415321/150‑1 (MQ=255)
                                          gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGc                                                                                                       <  2:1641253/150‑1 (MQ=255)
                                            aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCt                                                                                                     <  1:4851891/150‑1 (MQ=255)
                                            aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCt                                                                                                     <  2:4451285/150‑1 (MQ=255)
                                              tGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTac                                                                                                   >  2:3582123/1‑150 (MQ=255)
                                                  gTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACttttt                                                                                                                            <  1:634249/121‑1 (MQ=255)
                                                    tAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTc                                                                                             <  1:251119/150‑1 (MQ=255)
                                                      aaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                                                                                           >  2:4062404/1‑150 (MQ=255)
                                                         tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                        <  2:1239916/150‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                       <  2:2162928/150‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                       >  2:2506556/1‑150 (MQ=255)
                                                           aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                      >  2:1855595/1‑150 (MQ=255)
                                                           aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                      <  1:1376332/150‑1 (MQ=255)
                                                           aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                      <  1:3209378/150‑1 (MQ=255)
                                                             tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGcc                                                                                    >  2:2050206/1‑150 (MQ=255)
                                                             tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGcc                                                                                    >  1:1063239/1‑150 (MQ=255)
                                                               aTGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCgg                                                                                  <  2:3910976/150‑1 (MQ=255)
                                                                 gAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGtt                                                                                <  2:3548951/150‑1 (MQ=255)
                                                                  aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                               >  2:4740816/1‑150 (MQ=255)
                                                                  aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                               >  1:3637000/1‑150 (MQ=255)
                                                                     tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                            <  2:5117605/150‑1 (MQ=255)
                                                                          aaCTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTg                                                                       >  1:572852/1‑150 (MQ=255)
                                                                           actactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa                                                                      >  1:326465/1‑150 (MQ=255)
                                                                           actactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGa                                                                      >  1:4333239/1‑150 (MQ=255)
                                                                             tactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAACTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg                                                                    <  2:2923074/150‑1 (MQ=255)
                                                                             tactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg                                                                    >  1:2762830/1‑150 (MQ=255)
                                                                                  cGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc                                                               >  2:3010520/1‑150 (MQ=255)
                                                                                   gTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt                                                              >  1:3662236/1‑150 (MQ=255)
                                                                                    tCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa                                                             >  1:4437505/1‑150 (MQ=255)
                                                                                     cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAc                                                            >  2:166930/1‑150 (MQ=255)
                                                                                     cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAc                                                            >  1:202409/1‑150 (MQ=255)
                                                                                      tttGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg                                                           <  2:84889/150‑1 (MQ=255)
                                                                                        tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGtt                                                         <  1:4322841/150‑1 (MQ=255)
                                                                                           attaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGa                                                      >  2:3094673/3‑150 (MQ=255)
                                                                                           agtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGa                                                      <  1:475725/150‑1 (MQ=255)
                                                                                            gtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaa                                                     >  2:4968568/1‑150 (MQ=255)
                                                                                               aTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAcc                                                  >  2:633928/1‑150 (MQ=255)
                                                                                                 cATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTg                                                >  1:3754444/1‑150 (MQ=255)
                                                                                                     cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATa                                            >  1:1115705/1‑150 (MQ=255)
                                                                                                       gTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGc                                          >  2:3243654/1‑150 (MQ=255)
                                                                                                          tCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGc                                       >  2:1752727/1‑150 (MQ=255)
                                                                                                                 cGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                <  2:11605/150‑1 (MQ=255)
                                                                                                                 cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                <  1:2393995/150‑1 (MQ=255)
                                                                                                                 cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                >  1:4112615/1‑150 (MQ=255)
                                                                                                                   tactacTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                              >  2:3369568/1‑150 (MQ=255)
                                                                                                                    actactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCa                             >  2:1023656/1‑150 (MQ=255)
                                                                                                                    actactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCa                             >  1:4611189/1‑150 (MQ=255)
                                                                                                                         tCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGAt                        >  2:1136417/1‑150 (MQ=255)
                                                                                                                             aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                    <  1:1310660/150‑1 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTTAGACTTTAAGAAAAGGACTTTTTTACAGTATTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTTAATAGCGGCTTCTTTTTCAACGATGCCAcc                  <  1:1009590/150‑1 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAcc                  <  1:2257832/150‑1 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAcc                  >  1:2254384/1‑150 (MQ=255)
                                                                                                                                   ttGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGAAGCCACCCggt              >  2:3751399/1‑150 (MQ=255)
                                                                                                                                    tGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggtt             >  1:3995859/1‑150 (MQ=255)
                                                                                                                                      aTAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttgg           <  2:2319419/150‑1 (MQ=255)
                                                                                                                                       tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggt          <  2:4886939/150‑1 (MQ=255)
                                                                                                                                       tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggt          >  1:3522967/1‑150 (MQ=255)
                                                                                                                                        aGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggtt         <  1:1784909/150‑1 (MQ=255)
                                                                                                                                           ttCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAg      <  2:1862514/150‑1 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGTTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg     <  1:1622131/150‑1 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCatg     <  2:4291615/150‑3 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg     >  2:3766216/1‑150 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg     >  2:2841599/1‑150 (MQ=255)
                                                                                                                                              gCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGc   >  1:3080260/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCC  >  CP009273/3440685‑3440977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑