Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,504,143 G→T 39.8% R65S (CGT→AGT)  yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,504,1430GT39.8% 407.9 / inf 341R65S (CGT→AGT) yhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base G (117/88);  new base T (69/67);  total (186/155)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

AGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGG  >  CP009273/3503994‑3504292
                                                                                                                                                     |                                                                                                                                                     
aGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACg                                                                                                                                                       >  2:2293341/1‑150 (MQ=255)
  cAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATtactac                                                                                                                                                     <  1:1619350/150‑1 (MQ=255)
   aaCCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGAcc                                                                                                                                                    <  2:2390861/150‑1 (MQ=255)
     ccTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCaa                                                                                                                                                  >  2:665841/1‑150 (MQ=255)
      cTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCaaa                                                                                                                                                 >  2:3382185/1‑150 (MQ=255)
      cTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCaaa                                                                                                                                                 >  1:1018452/1‑150 (MQ=255)
      cTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCaaa                                                                                                                                                 >  2:3928457/1‑150 (MQ=255)
         atgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTg                                                                                                                                              >  1:4131721/1‑150 (MQ=255)
          tnaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGAGTACGACCAAACTGc                                                                                                                                             >  1:2606426/1‑150 (MQ=255)
             cAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTg                                                                                                                                          <  1:4595622/150‑1 (MQ=255)
             cAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTg                                                                                                                                          >  2:298214/1‑150 (MQ=255)
                    tttGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtcatca                                                                                                                                   <  2:227204/150‑1 (MQ=255)
                     ttGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAg                                                                                                                                  <  1:1158581/150‑1 (MQ=255)
                     ttGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAg                                                                                                                                  <  1:1794813/150‑1 (MQ=255)
                      tGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGa                                                                                                                                 >  1:3668734/1‑150 (MQ=255)
                         aCCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATAc                                                                                                                              <  2:4188545/150‑1 (MQ=255)
                                 ccccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAAt                                                                                                                      <  2:2040619/150‑1 (MQ=255)
                                 ccccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAAt                                                                                                                      >  1:2347990/1‑150 (MQ=255)
                                 ccccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAAt                                                                                                                      <  2:2940101/150‑1 (MQ=255)
                                  cccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATg                                                                                                                     >  2:4652890/1‑150 (MQ=255)
                                  cccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATg                                                                                                                     >  2:1549176/1‑150 (MQ=255)
                                  cccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATg                                                                                                                     <  1:3430909/150‑1 (MQ=255)
                                      gATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGt                                                                                                                 >  2:3194035/1‑150 (MQ=255)
                                      gATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGt                                                                                                                 <  2:2638034/150‑1 (MQ=255)
                                       aTGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTa                                                                                                                >  2:1298842/1‑150 (MQ=255)
                                         gcgcCACAGGCAAACAAGCGCGGCGCATGACTCGCGCCTCGTTGTAATCCACCGCCACAATGCCGCTGTACCCTAATGCCAGCAATATTTCCGCCAGCAACGGATTTCGACCAAACTGCTTGGTCATCAGATACTGATCAATGCCGTaaa                                                                                                              >  1:5134329/1‑150 (MQ=255)
                                          cgcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAg                                                                                                             >  1:449205/1‑150 (MQ=255)
                                           gcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGc                                                                                                            <  1:3334048/150‑1 (MQ=255)
                                            ccACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCACACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGcc                                                                                                           >  2:2819212/1‑150 (MQ=255)
                                            ccACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGcc                                                                                                           <  2:3869170/150‑1 (MQ=255)
                                            ccACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGcc                                                                                                           >  1:1310969/1‑150 (MQ=255)
                                              acaGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCcgc                                                                                                         >  2:3205863/1‑150 (MQ=255)
                                                aGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCgcgc                                                                                                       >  1:4844729/1‑150 (MQ=255)
                                                  gCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCg                                                                                                     <  2:514279/150‑1 (MQ=255)
                                                    aaaCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtc                                                                                                   >  2:1234363/1‑150 (MQ=255)
                                                      aCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtctc                                                                                                 <  1:2687185/150‑1 (MQ=255)
                                                      aCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtctc                                                                                                 <  1:3415824/150‑1 (MQ=255)
                                                       ccAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCa                                                                                                >  2:4753185/1‑150 (MQ=255)
                                                        cAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCaa                                                                                               >  1:3549255/1‑150 (MQ=255)
                                                        cAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCaa                                                                                               >  1:3137454/1‑150 (MQ=255)
                                                        cAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCaa                                                                                               >  1:3903519/1‑150 (MQ=255)
                                                         aGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAAt                                                                                              <  2:4281327/150‑1 (MQ=255)
                                                         aGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAAt                                                                                              <  2:4846905/150‑1 (MQ=255)
                                                           cgcgGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCa                                                                                            <  1:4166401/150‑1 (MQ=255)
                                                            gcggcgCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAg                                                                                           <  1:2100497/150‑1 (MQ=255)
                                                                cgcATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGc                                                                                       >  2:3811288/1‑150 (MQ=255)
                                                                  cATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCtt                                                                                     >  2:3017625/1‑150 (MQ=255)
                                                                     gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAcc                                                                                  >  2:2859910/1‑150 (MQ=255)
                                                                     gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAcc                                                                                  >  2:260724/1‑150 (MQ=255)
                                                                      aCTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCg                                                                                 <  1:3508932/150‑1 (MQ=255)
                                                                           cgcCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTc                                                                            <  1:2482556/150‑1 (MQ=255)
                                                                            gcCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                           >  1:889262/1‑150 (MQ=255)
                                                                             ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCa                                                                          <  2:1655130/150‑1 (MQ=255)
                                                                             ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCa                                                                          >  2:3139166/1‑150 (MQ=255)
                                                                                    gTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTg                                                                   >  1:2876854/1‑150 (MQ=255)
                                                                                     tAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGa                                                                  >  1:3032780/1‑150 (MQ=255)
                                                                                     tAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGa                                                                  <  2:81058/150‑1 (MQ=255)
                                                                                        tCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATcc                                                               <  1:3234459/150‑1 (MQ=255)
                                                                                         ccACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCa                                                              <  2:3731841/150‑1 (MQ=255)
                                                                                           aCCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACg                                                            >  2:722345/1‑150 (MQ=255)
                                                                                           aCCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACg                                                            >  2:928188/1‑150 (MQ=255)
                                                                                            ccGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGt                                                           <  2:400159/150‑1 (MQ=255)
                                                                                              gCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCg                                                         >  2:5046071/1‑150 (MQ=255)
                                                                                               ccACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGa                                                        >  1:1589902/1‑150 (MQ=255)
                                                                                                cacaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGAt                                                       <  1:2411969/150‑1 (MQ=255)
                                                                                                cacaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGAt                                                       >  2:1567252/1‑150 (MQ=255)
                                                                                                cacaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGAATCTGATCCACGTCGAt                                                       >  2:4384331/1‑150 (MQ=255)
                                                                                                  caATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCa                                                     >  2:396546/1‑150 (MQ=255)
                                                                                                   aaTGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCAc                                                    <  2:4843049/150‑1 (MQ=255)
                                                                                                        cGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGc                                               >  1:4958783/1‑150 (MQ=255)
                                                                                                         gCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCt                                              >  2:1147402/1‑150 (MQ=255)
                                                                                                           tGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGt                                            <  2:3533423/150‑1 (MQ=255)
                                                                                                               gCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCgg                                        <  2:2455741/150‑1 (MQ=255)
                                                                                                                  tAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATTCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGc                                     <  2:4777897/150‑1 (MQ=255)
                                                                                                                  tAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGc                                     <  1:1245595/150‑1 (MQ=255)
                                                                                                                  tAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGc                                     <  1:1001550/150‑1 (MQ=255)
                                                                                                                     tGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGGATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGAt                                  >  1:37633/1‑150 (MQ=255)
                                                                                                                     tGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGAt                                  >  1:5147828/1‑150 (MQ=255)
                                                                                                                          gCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGc                             <  2:270267/150‑1 (MQ=255)
                                                                                                                           cAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGcc                            <  2:3606138/150‑1 (MQ=255)
                                                                                                                           cAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGcc                            <  2:1061600/150‑1 (MQ=255)
                                                                                                                            aaTTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGccc                           >  2:3332572/1‑150 (MQ=255)
                                                                                                                              tttttCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctg                         <  1:135522/150‑1 (MQ=255)
                                                                                                                              tttttCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctg                         >  2:1965451/1‑150 (MQ=255)
                                                                                                                               ttttCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctgc                        >  1:1660912/1‑150 (MQ=255)
                                                                                                                               ttttCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctgc                        >  1:3964627/1‑150 (MQ=255)
                                                                                                                                ttttCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctgct                       <  2:1775822/150‑1 (MQ=255)
                                                                                                                                       cagccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAg                <  2:1438957/150‑1 (MQ=255)
                                                                                                                                       cagccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAg                >  2:4090451/1‑150 (MQ=255)
                                                                                                                                        agccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAgg               >  2:3104437/1‑150 (MQ=255)
                                                                                                                                         gccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGc              >  1:2571845/1‑150 (MQ=255)
                                                                                                                                         gccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTCCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATATGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGc              >  1:3109441/1‑150 (MQ=255)
                                                                                                                                          ccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCt             >  2:1721212/1‑150 (MQ=255)
                                                                                                                                           caCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCtt            >  1:812950/1‑150 (MQ=255)
                                                                                                                                              ggATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTAAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCTATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGt         >  1:618203/1‑150 (MQ=255)
                                                                                                                                               gATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTg        >  1:4538169/1‑150 (MQ=255)
                                                                                                                                                 ttACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcg      >  1:2255404/1‑150 (MQ=255)
                                                                                                                                                    cGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcggcg   >  1:476155/1‑150 (MQ=255)
                                                                                                                                                     gACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGcggcgg  >  2:4668902/1‑150 (MQ=255)
                                                                                                                                                     gACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGcggcgg  >  1:679394/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGG  >  CP009273/3503994‑3504292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑