Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,542,744 T→A 10.2% Q229L (CAG→CTG)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,542,7440TA10.2% 1138.7 / 92.6 350Q229L (CAG→CTG) malQ4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base T (148/166);  new base A (15/21);  total (163/187)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

CCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGAC  >  CP009273/3542595‑3542893
                                                                                                                                                     |                                                                                                                                                     
ccATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCt                                                                                                                                                       <  1:4437277/150‑1 (MQ=255)
ccATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCt                                                                                                                                                       <  1:5012123/150‑1 (MQ=255)
    cTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGc                                                                                                                                                   >  2:4557643/1‑150 (MQ=255)
      gCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCct                                                                                                                                                 <  2:2549539/150‑1 (MQ=255)
          atcatcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTc                                                                                                                                             >  2:4865493/1‑150 (MQ=255)
          atcatcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTc                                                                                                                                             <  1:637341/150‑1 (MQ=255)
           tcatcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCg                                                                                                                                            <  1:1334835/150‑1 (MQ=255)
             atcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCt                                                                                                                                          <  1:3133029/150‑1 (MQ=255)
             atcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCt                                                                                                                                          >  2:1513885/1‑150 (MQ=255)
                aCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAg                                                                                                                                       >  2:5055878/1‑150 (MQ=255)
                   ttGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATg                                                                                                                                    >  1:1166376/1‑150 (MQ=255)
                    tGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATgg                                                                                                                                   <  2:4723740/150‑1 (MQ=255)
                       gcgAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGaaa                                                                                                                                >  1:1066821/1‑150 (MQ=255)
                        cgAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAAt                                                                                                                               >  2:2408991/1‑150 (MQ=255)
                         gAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATc                                                                                                                              <  2:49617/150‑1 (MQ=255)
                          aaaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCt                                                                                                                             <  1:1463562/150‑1 (MQ=255)
                          aaaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCt                                                                                                                             <  2:4712228/150‑1 (MQ=255)
                          aaaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCt                                                                                                                             <  1:544732/150‑1 (MQ=255)
                           aaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCtt                                                                                                                            <  1:565339/150‑1 (MQ=255)
                           aaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTAGCTAAGATGGAAATCtt                                                                                                                            <  2:116477/150‑1 (MQ=255)
                            aCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGAAGGAAATCTTc                                                                                                                           <  1:4494963/150‑1 (MQ=255)
                               tttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAAc                                                                                                                        <  1:30722/150‑1 (MQ=255)
                               tttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAAc                                                                                                                        <  2:2447921/150‑1 (MQ=255)
                                ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACg                                                                                                                       >  1:5033904/1‑150 (MQ=255)
                                  ccACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGc                                                                                                                     <  1:4880984/150‑1 (MQ=255)
                                   cACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCg                                                                                                                    >  1:1265706/1‑150 (MQ=255)
                                     cGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGtt                                                                                                                  >  2:1013146/1‑150 (MQ=255)
                                      gCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTa                                                                                                                 >  2:1801946/1‑150 (MQ=255)
                                       ccATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTaa                                                                                                                >  1:3227172/1‑150 (MQ=255)
                                        cATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAAc                                                                                                               <  2:2587404/150‑1 (MQ=255)
                                         aTTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACg                                                                                                              >  1:2350785/1‑150 (MQ=255)
                                         aTTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACg                                                                                                              >  1:2472879/1‑150 (MQ=255)
                                           tCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTc                                                                                                            <  1:1968330/150‑1 (MQ=255)
                                               attGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGata                                                                                                        <  2:3272519/148‑1 (MQ=255)
                                               aaTGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGata                                                                                                        <  2:5062970/150‑1 (MQ=255)
                                               aaTGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGata                                                                                                        >  2:3129851/1‑150 (MQ=255)
                                                 tGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGAtata                                                                                                      <  1:1663146/150‑1 (MQ=255)
                                                 tGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGAtata                                                                                                      <  1:4636736/150‑1 (MQ=255)
                                                  gCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATaa                                                                                                     <  2:1209232/150‑1 (MQ=255)
                                                   cTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATaaa                                                                                                    <  2:1930542/150‑1 (MQ=255)
                                                   cTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATaaa                                                                                                    >  2:3087379/1‑150 (MQ=255)
                                                      tCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATca                                                                                                 <  2:3612100/150‑1 (MQ=255)
                                                      tCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATca                                                                                                 >  1:3846482/1‑150 (MQ=255)
                                                       cATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATcac                                                                                                >  2:4326042/1‑150 (MQ=255)
                                                          ttttAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACAtt                                                                                             <  1:66207/150‑1 (MQ=255)
                                                           tttAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTc                                                                                            >  2:717499/1‑150 (MQ=255)
                                                             tAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGGGGCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAg                                                                                          <  2:2339421/150‑1 (MQ=255)
                                                              aGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGc                                                                                         <  2:613476/150‑1 (MQ=255)
                                                              aGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGc                                                                                         >  2:902446/1‑150 (MQ=255)
                                                               gggCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGGAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGcc                                                                                        >  1:3641291/1‑150 (MQ=255)
                                                                 gCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCaa                                                                                      >  1:2648720/1‑150 (MQ=255)
                                                                  cGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAAc                                                                                     >  1:171558/1‑150 (MQ=255)
                                                                   ggTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACg                                                                                    <  2:1260828/150‑1 (MQ=255)
                                                                    gTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACgg                                                                                   >  2:1591075/1‑150 (MQ=255)
                                                                    gTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACgg                                                                                   <  2:1096389/150‑1 (MQ=255)
                                                                       aCCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCga                                                                                >  1:1570293/1‑150 (MQ=255)
                                                                       aCCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCga                                                                                >  2:19349/1‑150 (MQ=255)
                                                                         cGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCgaga                                                                              >  2:2025854/1‑150 (MQ=255)
                                                                         cGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCgaga                                                                              <  2:4814775/150‑1 (MQ=255)
                                                                         cGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGGTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCgaga                                                                              <  2:2280633/150‑1 (MQ=255)
                                                                          gTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGagaa                                                                             <  1:70546/150‑1 (MQ=255)
                                                                           tGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGagaag                                                                            <  1:3454889/150‑1 (MQ=255)
                                                                              aGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACg                                                                         >  1:3344586/1‑150 (MQ=255)
                                                                               gTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACgg                                                                        <  1:2970994/150‑1 (MQ=255)
                                                                               gTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACgg                                                                        >  2:2826391/1‑150 (MQ=255)
                                                                                tAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACggg                                                                       >  1:4745846/1‑150 (MQ=255)
                                                                                 aaTCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGc                                                                      <  1:76857/150‑1 (MQ=255)
                                                                                   tCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTg                                                                    <  2:4354305/150‑1 (MQ=255)
                                                                                   tCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTg                                                                    <  2:1330146/150‑1 (MQ=255)
                                                                                    cGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGt                                                                   >  1:2593012/1‑150 (MQ=255)
                                                                                          aGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGc                                                             >  2:3385541/1‑150 (MQ=255)
                                                                                           gTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCt                                                            <  1:4045616/150‑1 (MQ=255)
                                                                                           gTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCt                                                            <  1:4834579/150‑1 (MQ=255)
                                                                                              ggCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGgc                                                         <  1:2566987/150‑1 (MQ=255)
                                                                                              ggCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGgc                                                         >  1:1883717/1‑150 (MQ=255)
                                                                                                     cgcgcCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTcc                                                  >  2:4181257/1‑150 (MQ=255)
                                                                                                     cgcgcCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTcc                                                  <  2:3327686/150‑1 (MQ=255)
                                                                                                        gcCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGa                                               >  2:1092876/1‑150 (MQ=255)
                                                                                                        gcCTGCTGCAGCGTCTGTTGCGTGGTCGGCAACGGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGa                                               <  2:3489978/150‑1 (MQ=255)
                                                                                                         ccTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGAt                                              >  1:3294705/1‑150 (MQ=255)
                                                                                                           tGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGAttt                                            >  2:1206002/1‑150 (MQ=255)
                                                                                                            gTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTACGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTg                                           <  2:3859625/150‑1 (MQ=255)
                                                                                                             ttGCAGCGTCTGGTGCGTGGTCGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGc                                          <  2:4673288/150‑1 (MQ=255)
                                                                                                                   cGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATa                                    >  1:426321/1‑150 (MQ=255)
                                                                                                                          tGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCa                             <  1:2104321/150‑1 (MQ=255)
                                                                                                                             gTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGa                          <  2:4121044/150‑1 (MQ=255)
                                                                                                                               ggTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAAt                        <  2:1714816/150‑1 (MQ=255)
                                                                                                                               ggTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAAt                        <  1:2251658/150‑1 (MQ=255)
                                                                                                                                  cGGCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCgg                     <  2:1445631/150‑1 (MQ=255)
                                                                                                                                    gCAACTGCCACCAGGCCAGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGt                   <  2:1665831/150‑1 (MQ=255)
                                                                                                                                      aaCTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTc                 >  1:4198255/1‑150 (MQ=255)
                                                                                                                                      aaCTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTc                 <  2:2144772/150‑1 (MQ=255)
                                                                                                                                       aCTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCa                <  2:269305/150‑1 (MQ=255)
                                                                                                                                         tGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAgg              <  1:4385836/150‑1 (MQ=255)
                                                                                                                                            caccaGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCa           >  1:2763297/1‑150 (MQ=255)
                                                                                                                                             accaGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTCTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCaa          >  2:2746942/1‑150 (MQ=255)
                                                                                                                                              ccaGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATCGAGCGCATGAATCGGGTTCAGGCCAAt         >  1:2950933/1‑150 (MQ=255)
                                                                                                                                              ccaGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAAt         >  1:1790632/1‑150 (MQ=255)
                                                                                                                                               caGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATg        >  1:3411/1‑150 (MQ=255)
                                                                                                                                                  gCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAAc     <  1:1770772/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGAC  >  CP009273/3542595‑3542893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑