Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,557,815 T→A 9.0% K709N (AAA→AAT)  glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,557,8150TA 9.0% 1146.0 / 79.9 346K709N (AAA→AAT) glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (132/183);  new base A (16/15);  total (148/198)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 354 total aligned reads displayed.

TCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGT  >  CP009273/3557666‑3557963
                                                                                                                                                     |                                                                                                                                                    
tCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGt                                                                                                                                                      <  2:84307/150‑1 (MQ=255)
 ccGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGtt                                                                                                                                                     <  1:664077/150‑1 (MQ=255)
 ccGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGtt                                                                                                                                                     >  1:5129415/1‑150 (MQ=255)
  cGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGttt                                                                                                                                                    >  1:1161731/1‑150 (MQ=255)
  cGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGttt                                                                                                                                                    >  1:4879840/1‑150 (MQ=255)
    ccAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGt                                                                                                                                                  >  2:1221755/1‑150 (MQ=255)
    ccAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGt                                                                                                                                                  <  1:2704737/150‑1 (MQ=255)
     cAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTGTGTa                                                                                                                                                 >  1:3890454/1‑150 (MQ=255)
       gTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGc                                                                                                                                               >  2:313371/1‑150 (MQ=255)
       gTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGc                                                                                                                                               <  2:2567687/150‑1 (MQ=255)
        tACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGcc                                                                                                                                              >  1:2940379/1‑150 (MQ=255)
            tGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga                                                                                                                                          <  2:2194847/150‑1 (MQ=255)
            tGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGAGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga                                                                                                                                          <  1:498758/150‑1 (MQ=255)
            tGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGAGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga                                                                                                                                          <  1:928948/150‑1 (MQ=255)
                aGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacgac                                                                                                                                      <  1:2065285/150‑1 (MQ=255)
                aGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacgac                                                                                                                                      <  1:2038727/150‑1 (MQ=255)
                  tGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGc                                                                                                                                    >  2:1776698/1‑150 (MQ=255)
                   gATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCa                                                                                                                                   >  2:4222837/1‑150 (MQ=255)
                          gAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGttcttc                                                                                                                            >  2:3047543/1‑150 (MQ=255)
                           aaGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTcc                                                                                                                           <  1:1842531/150‑1 (MQ=255)
                           aaGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTcc                                                                                                                           <  2:2752709/150‑1 (MQ=255)
                            aGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCa                                                                                                                          >  2:2448048/1‑150 (MQ=255)
                             gTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCAc                                                                                                                         <  1:1080413/150‑1 (MQ=255)
                               tGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActt                                                                                                                       <  1:1766631/150‑1 (MQ=255)
                                gATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtc                                                                                                                      >  2:1869488/1‑150 (MQ=255)
                                 aTCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtct                                                                                                                     >  2:3797298/1‑150 (MQ=255)
                                  tCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtctt                                                                                                                    <  2:3587721/150‑1 (MQ=255)
                                    aGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTcc                                                                                                                  <  2:5058963/150‑1 (MQ=255)
                                      cGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGc                                                                                                                >  2:1797282/1‑150 (MQ=255)
                                      cGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGc                                                                                                                <  2:1578037/150‑1 (MQ=255)
                                       gAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCt                                                                                                               >  2:4599438/1‑150 (MQ=255)
                                         aTCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCtgt                                                                                                             <  2:3654440/150‑1 (MQ=255)
                                           cAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTgtgt                                                                                                           <  1:2407700/150‑1 (MQ=255)
                                            aaCCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGtt                                                                                                          <  2:208257/150‑1 (MQ=255)
                                              ccAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTAc                                                                                                        <  2:557629/150‑1 (MQ=255)
                                              ccAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTAc                                                                                                        <  1:3635470/150‑1 (MQ=255)
                                                aGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCa                                                                                                      >  1:3857940/1‑150 (MQ=255)
                                                  aTCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCaaa                                                                                                    <  1:4546785/150‑1 (MQ=255)
                                                  aTCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCaaa                                                                                                    <  2:2588888/150‑1 (MQ=255)
                                                    cgcgATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCaaaaa                                                                                                  >  1:3689437/1‑150 (MQ=255)
                                                        aTAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATaaa                                                                                              <  2:3361762/150‑1 (MQ=255)
                                                         tAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAg                                                                                             >  1:2588949/1‑150 (MQ=255)
                                                           gCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGat                                                                                           <  1:2171977/150‑1 (MQ=255)
                                                           gCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGat                                                                                           <  1:2853290/150‑1 (MQ=255)
                                                            cGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGata                                                                                          <  2:3445700/150‑1 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                         >  2:4399787/1‑150 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                         >  1:1284982/1‑150 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                         <  2:4058450/150‑1 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                         <  1:3454152/150‑1 (MQ=255)
                                                              aCCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATAtt                                                                                        <  2:1598657/150‑1 (MQ=255)
                                                              aCCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATAtt                                                                                        <  1:577484/150‑1 (MQ=255)
                                                                 cGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTc                                                                                     >  1:4727824/1‑150 (MQ=255)
                                                                  ggATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCa                                                                                    >  1:4581767/1‑150 (MQ=255)
                                                                     tCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCa                                                                                 >  1:1945603/1‑150 (MQ=255)
                                                                     tCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCa                                                                                 <  1:3382297/150‑1 (MQ=255)
                                                                         ccGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGa                                                                             >  1:2311069/1‑150 (MQ=255)
                                                                          cGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGAc                                                                            <  1:2200655/150‑1 (MQ=255)
                                                                            gACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACAt                                                                          >  2:4267663/1‑150 (MQ=255)
                                                                             aCTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATg                                                                         >  2:712226/1‑150 (MQ=255)
                                                                              cTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGa                                                                        >  1:3135223/1‑150 (MQ=255)
                                                                                gAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATc                                                                      >  2:211311/1‑150 (MQ=255)
                                                                                gAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATc                                                                      <  2:1828613/150‑1 (MQ=255)
                                                                                       cctcTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAtc                                                               >  1:1525194/4‑150 (MQ=255)
                                                                                        cGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAtct                                                              >  2:3224273/1‑150 (MQ=255)
                                                                                          cTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCg                                                            <  2:239297/150‑1 (MQ=255)
                                                                                          cTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGAACCGACATGATCCAGCATCTCg                                                            <  1:2776774/150‑1 (MQ=255)
                                                                                           tGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGa                                                           <  1:3034173/150‑1 (MQ=255)
                                                                                                 tttGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCg                                                     >  2:4123052/1‑150 (MQ=255)
                                                                                                 tttGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCg                                                     <  1:4314970/150‑1 (MQ=255)
                                                                                                      gTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCg                                                >  1:4220698/1‑150 (MQ=255)
                                                                                                           cACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCaa                                           >  1:2672252/1‑150 (MQ=255)
                                                                                                               tGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTa                                       >  1:5035074/1‑150 (MQ=255)
                                                                                                                 aTGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTAcc                                     <  1:1019381/150‑1 (MQ=255)
                                                                                                                   gCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGa                                   >  2:1086272/1‑150 (MQ=255)
                                                                                                                    cAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGAt                                  >  2:2271607/1‑150 (MQ=255)
                                                                                                                       ctcctcATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCa                                            >  2:807802/1‑137 (MQ=255)
                                                                                                                       ctcctcATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCa                                            <  1:807802/137‑1 (MQ=255)
                                                                                                                        tcctcATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTc                              >  2:1224450/1‑150 (MQ=255)
                                                                                                                        tcctcATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTc                              >  2:1693915/1‑150 (MQ=255)
                                                                                                                         cctcATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACAGTCGCACCGTCCAACGTACCGATAGTCa                             <  2:4174065/150‑1 (MQ=255)
                                                                                                                            cATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAgcg                          >  2:3743348/1‑150 (MQ=255)
                                                                                                                              tCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCa                        <  2:47441/150‑1 (MQ=255)
                                                                                                                                tttCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCAcc                      >  1:4839120/1‑150 (MQ=255)
                                                                                                                                 ttCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCCGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCg                     >  1:4625117/1‑150 (MQ=255)
                                                                                                                                   ctcGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGtt                   <  1:1423859/150‑1 (MQ=255)
                                                                                                                                    tcGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTa                  <  1:382279/150‑1 (MQ=255)
                                                                                                                                    tcGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACGTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTa                  <  1:405620/150‑1 (MQ=255)
                                                                                                                                     cGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATCAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGGCAGCGCCCCGGTac                 >  1:3109110/1‑149 (MQ=255)
                                                                                                                                      gtagtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAg                <  2:3890612/150‑1 (MQ=255)
                                                                                                                                      gtagtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAg                >  1:1689023/1‑150 (MQ=255)
                                                                                                                                        agtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAgcg              <  1:3846121/150‑1 (MQ=255)
                                                                                                                                        agtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAgcg              <  1:754114/150‑1 (MQ=255)
                                                                                                                                          taTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCa            <  2:946002/150‑1 (MQ=255)
                                                                                                                                          taTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCa            >  1:2372583/1‑150 (MQ=255)
                                                                                                                                           aTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCaa           <  1:3330912/150‑1 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATg   <  2:5007300/150‑1 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATg   >  1:3190873/1‑150 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATg   >  1:3274901/1‑150 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATg   >  2:2872016/1‑150 (MQ=255)
                                                                                                                                                    gTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGt  <  1:357669/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGT  >  CP009273/3557666‑3557963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑