Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,588,904 A→T 26.9% L287Q (CTG→CAG)  livH ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,588,9040AT26.9% 543.8 / 239.2 286L287Q (CTG→CAG) livHleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (118/91);  new base T (33/44);  total (151/135)
Fisher's exact test for biased strand distribution p-value = 4.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGC  >  CP009273/3588755‑3589052
                                                                                                                                                     |                                                                                                                                                    
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      <  1:3308335/150‑1 (MQ=255)
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      <  2:1645235/150‑1 (MQ=255)
 ccAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagcag                                                                                                                                                     >  2:1401916/1‑150 (MQ=255)
      tGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGa                                                                                                                                                >  2:1148888/1‑150 (MQ=255)
      tGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGa                                                                                                                                                >  1:1501504/1‑150 (MQ=255)
           gCCCATAAAGACGCCCGCCAGCACAAAGAACCTCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTGTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATgag                                                                                                                                           <  2:1443653/150‑1 (MQ=255)
            cccATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATgaga                                                                                                                                          <  1:589227/150‑1 (MQ=255)
               aTAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACca                                                                                                                                       >  1:4922799/1‑150 (MQ=255)
                tAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACcac                                                                                                                                      >  1:4503318/1‑150 (MQ=255)
                tAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACcac                                                                                                                                      <  2:1483908/150‑1 (MQ=255)
                  aaGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACAt                                                                                                                                    <  1:1654897/150‑1 (MQ=255)
                  aaGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACAt                                                                                                                                    <  1:3469571/150‑1 (MQ=255)
                  aaGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACAt                                                                                                                                    <  1:2741473/150‑1 (MQ=255)
                     aCGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCtt                                                                                                                                 >  1:1193535/1‑150 (MQ=255)
                       gcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTta                                                                                                                               >  1:1292113/1‑150 (MQ=255)
                       gcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTta                                                                                                                               >  1:924260/1‑150 (MQ=255)
                          cgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTatat                                                                                                                            >  1:2217411/1‑150 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGACTGAGACCACATCTTGATATTc                                                                                                                          >  2:2909625/1‑150 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTc                                                                                                                          <  1:4619842/150‑1 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTc                                                                                                                          >  1:2803197/1‑150 (MQ=255)
                             cAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTcc                                                                                                                         <  1:983181/150‑1 (MQ=255)
                             cAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTcc                                                                                                                         <  2:2258310/150‑1 (MQ=255)
                              aGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCg                                                                                                                        <  2:4416213/150‑1 (MQ=255)
                               gCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGt                                                                                                                       <  2:326732/150‑1 (MQ=255)
                                   aaaGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTc                                                                                                                   <  2:4151449/150‑1 (MQ=255)
                                    aaGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGACTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCa                                                                                                                  <  2:3555489/150‑1 (MQ=255)
                                    aaGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCa                                                                                                                  >  1:2644401/1‑150 (MQ=255)
                                      gAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGa                                                                                                                >  1:1986048/1‑150 (MQ=255)
                                        aCATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATa                                                                                                              <  2:4006966/150‑1 (MQ=255)
                                          aTCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAgg                                                                                                            >  2:4957512/1‑150 (MQ=255)
                                          aTCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAgg                                                                                                            >  1:2071217/1‑150 (MQ=255)
                                          aTCGCGGCAGAGAGCAGCGCCATTGCAATAGGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGACTGAGACCCCATCTTTATATTCCGGACTCAGATAgg                                                                                                            >  2:3437189/1‑150 (MQ=255)
                                           tCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGc                                                                                                           <  2:3213932/150‑1 (MQ=255)
                                           tCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGc                                                                                                           <  2:2079960/150‑1 (MQ=255)
                                           tCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGc                                                                                                           <  2:500238/150‑1 (MQ=255)
                                              cgGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCaga                                                                                                        <  2:2046634/150‑1 (MQ=255)
                                                gCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaag                                                                                                      <  1:4543492/150‑1 (MQ=255)
                                                   gagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagaga                                                                                                   <  2:627962/150‑1 (MQ=255)
                                                   gagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagaga                                                                                                   <  1:3070586/150‑1 (MQ=255)
                                                   gagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagaga                                                                                                   >  2:3619668/1‑150 (MQ=255)
                                                    agagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagagag                                                                                                  >  1:1949144/1‑150 (MQ=255)
                                                          gcgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTc                                                                                            >  2:4697341/1‑150 (MQ=255)
                                                          gcgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTc                                                                                            >  1:628825/1‑150 (MQ=255)
                                                           cgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                           <  1:1151964/150‑1 (MQ=255)
                                                           cgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                           <  2:1424151/150‑1 (MQ=255)
                                                           cgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                           <  2:669080/150‑1 (MQ=255)
                                                           cgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                           <  2:2551441/150‑1 (MQ=255)
                                                            gcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCg                                                                                          >  1:527233/1‑150 (MQ=255)
                                                              cATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCa                                                                                        >  2:3835618/1‑150 (MQ=255)
                                                              cATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCa                                                                                        >  1:883074/1‑150 (MQ=255)
                                                                ttGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAAt                                                                                      >  1:1543770/1‑150 (MQ=255)
                                                                 tGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATc                                                                                     >  2:1209944/1‑150 (MQ=255)
                                                                 tGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATc                                                                                     <  1:168979/150‑1 (MQ=255)
                                                                   cAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATccc                                                                                   >  2:2373352/1‑150 (MQ=255)
                                                                        tGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGaa                                                                              >  2:2691806/1‑150 (MQ=255)
                                                                            tCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAg                                                                          >  1:4362378/1‑150 (MQ=255)
                                                                              ggTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgc                                                                        <  1:4245497/150‑1 (MQ=255)
                                                                              ggTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgc                                                                        >  2:5053471/1‑150 (MQ=255)
                                                                               gTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgcc                                                                       <  1:4455989/150‑1 (MQ=255)
                                                                                tttCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgccg                                                                      <  1:2507215/150‑1 (MQ=255)
                                                                                    aTACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCaa                                                                  >  1:3347793/1‑150 (MQ=255)
                                                                                     tACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTAAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAat                                                                 >  1:761404/1‑150 (MQ=255)
                                                                                         ttCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcatc                                                             >  1:1055720/1‑150 (MQ=255)
                                                                                           ctctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGc                                                           >  2:4262447/1‑150 (MQ=255)
                                                                                            tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCt                                                          <  1:1767382/150‑1 (MQ=255)
                                                                                               aCCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTccc                                                       >  1:4719667/1‑150 (MQ=255)
                                                                                                ccTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCg                                                      <  1:650854/150‑1 (MQ=255)
                                                                                                ccTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCg                                                      >  1:1392135/1‑150 (MQ=255)
                                                                                                 cTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCgg                                                     >  1:5061023/1‑150 (MQ=255)
                                                                                                   ccGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGaa                                                   >  2:458842/1‑150 (MQ=255)
                                                                                                     gggCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtg                                                 >  1:3211620/1‑150 (MQ=255)
                                                                                                       gCGACCCAGAAGACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgct                                               <  1:1955508/150‑1 (MQ=255)
                                                                                                        cGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgctg                                              >  2:1287574/1‑150 (MQ=255)
                                                                                                         gACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgctgc                                             >  2:10431/1‑150 (MQ=255)
                                                                                                            ccAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCaa                                          <  1:39495/150‑1 (MQ=255)
                                                                                                             cAGACTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAAt                                         <  2:908973/150‑1 (MQ=255)
                                                                                                             cAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAAt                                         >  1:4738084/1‑150 (MQ=255)
                                                                                                              aGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATc                                        >  2:4824543/1‑150 (MQ=255)
                                                                                                                aaTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATccc                                      <  2:4519249/150‑1 (MQ=255)
                                                                                                                aaTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATccc                                      >  1:3831304/1‑150 (MQ=255)
                                                                                                                aaTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATccc                                      <  2:4098037/150‑1 (MQ=255)
                                                                                                                 aTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCa                                     <  2:2458152/150‑1 (MQ=255)
                                                                                                                 aTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCa                                     >  2:3051176/1‑150 (MQ=255)
                                                                                                                    ccGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCg                                  <  1:3925331/150‑1 (MQ=255)
                                                                                                                    ccGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCg                                  >  1:4080146/1‑150 (MQ=255)
                                                                                                                     cGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGACTCAGGCCGCCAATCATCGCTCCCGGACTGCTGCCAATCCCACCGa                                 >  1:677363/1‑150 (MQ=255)
                                                                                                                         cGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAc                             <  1:2869389/150‑1 (MQ=255)
                                                                                                                          ggCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAcc                            >  1:1030822/1‑150 (MQ=255)
                                                                                                                           gCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAccg                           >  1:2175624/1‑150 (MQ=255)
                                                                                                                            cATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAccgc                          >  2:3978533/1‑150 (MQ=255)
                                                                                                                                  cagcagCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGt                    >  1:3604697/1‑150 (MQ=255)
                                                                                                                                   agcagcACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTa                   >  2:1902847/1‑150 (MQ=255)
                                                                                                                                    gcagcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTaa                  >  1:1739205/1‑150 (MQ=255)
                                                                                                                                     cagcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTaaa                 >  2:4214975/1‑150 (MQ=255)
                                                                                                                                         aCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCt             >  1:4119337/1‑150 (MQ=255)
                                                                                                                                            aGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGACTCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTc          >  2:3740915/1‑150 (MQ=255)
                                                                                                                                            aGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTc          >  1:2539570/1‑150 (MQ=255)
                                                                                                                                             gAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCa         >  2:1751576/1‑150 (MQ=255)
                                                                                                                                                  agctgGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCg    <  1:434059/150‑1 (MQ=255)
                                                                                                                                                  agcagGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCg    <  2:2803197/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGC  >  CP009273/3588755‑3589052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑