Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,589,238 G→T 5.5% R176S (CGC→AGC)  livH ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,589,2380GT 5.5% 1379.7 / 35.0 346R176S (CGC→AGC) livHleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (163/164);  new base T (12/7);  total (175/171)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 354 total aligned reads displayed.

CTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTT  >  CP009273/3589089‑3589387
                                                                                                                                                     |                                                                                                                                                     
cTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCg                                                                                                                                                       >  2:3100763/1‑150 (MQ=255)
 tGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGa                                                                                                                                                      <  1:4840205/150‑1 (MQ=255)
    ccGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATg                                                                                                                                                   <  2:5012408/150‑1 (MQ=255)
     cGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGa                                                                                                                                                  >  2:4165981/1‑150 (MQ=255)
       agcagcACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGaaa                                                                                                                                                >  1:1264048/1‑150 (MQ=255)
       agcagcACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGaaa                                                                                                                                                <  1:191298/150‑1 (MQ=255)
          agcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATc                                                                                                                                             >  2:3298378/1‑150 (MQ=255)
          agcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATc                                                                                                                                             <  2:4543585/150‑1 (MQ=255)
           gcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCg                                                                                                                                            <  1:5037801/150‑1 (MQ=255)
            cacaCCCGCCACCGACGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCAAGACACGAGCGACCCATGCGGGAATAGCGAATGAAAATCGt                                                                                                                                           >  2:3359963/1‑150 (MQ=255)
             acacCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                                                          >  2:2935575/1‑150 (MQ=255)
               acCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAg                                                                                                                                        >  1:1162701/1‑150 (MQ=255)
                 ccGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAgcg                                                                                                                                      >  1:2507682/1‑150 (MQ=255)
                    ccACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCa                                                                                                                                   <  2:4788539/150‑1 (MQ=255)
                    ccACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCa                                                                                                                                   <  1:458648/150‑1 (MQ=255)
                     cACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAg                                                                                                                                  <  1:270611/150‑1 (MQ=255)
                        cgccgcCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCTAATGAAAATCGTCAGCGCCAGCAt                                                                                                                               <  1:4214975/150‑1 (MQ=255)
                          ccgccATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATgg                                                                                                                             <  2:1240775/150‑1 (MQ=255)
                          ccgccATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATgg                                                                                                                             <  2:593132/150‑1 (MQ=255)
                            gccATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                                                           >  2:764918/1‑150 (MQ=255)
                              cATCGCCGCGCCAATCACACAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAg                                                                                                                         <  1:2170839/150‑1 (MQ=255)
                               aTCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAgg                                                                                                                        >  1:519586/1‑150 (MQ=255)
                               aTCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAgg                                                                                                                        <  1:2215642/150‑1 (MQ=255)
                                 cgccgcGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCTAATGAAAATCGTCAGCGCCAGCATGGCGAGGaa                                                                                                                      >  2:3388354/1‑150 (MQ=255)
                                       gcCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAAc                                                                                                                >  1:4404830/1‑150 (MQ=255)
                                        ccAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                                                               <  2:2577725/150‑1 (MQ=255)
                                         cAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCCTTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACaa                                                                                                              >  2:507041/1‑150 (MQ=255)
                                         cAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACaa                                                                                                              >  2:3059463/1‑150 (MQ=255)
                                             cacaAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCa                                                                                                          <  2:3604697/150‑1 (MQ=255)
                                               caAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGa                                                                                                        >  1:2575938/1‑150 (MQ=255)
                                                   ggTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                                                    >  2:1795855/1‑150 (MQ=255)
                                                    gTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAcc                                                                                                   <  2:4264113/150‑1 (MQ=255)
                                                    gTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAcc                                                                                                   <  1:3978533/150‑1 (MQ=255)
                                                    gTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAcc                                                                                                   <  2:568714/150‑1 (MQ=255)
                                                        gcgcAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCt                                                                                               >  2:4824913/1‑150 (MQ=255)
                                                         cgcAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTg                                                                                              <  1:3932138/150‑1 (MQ=255)
                                                          gcAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGc                                                                                             >  1:1481362/1‑150 (MQ=255)
                                                              tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtgg                                                                                         >  2:2223759/1‑150 (MQ=255)
                                                              tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtgg                                                                                         >  2:19634/1‑150 (MQ=255)
                                                              tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtgg                                                                                         >  1:3081424/1‑150 (MQ=255)
                                                                aCCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtggtg                                                                                       <  1:574848/150‑1 (MQ=255)
                                                                  ccGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATggtggt                                                                                     <  1:3736510/150‑1 (MQ=255)
                                                                  ccGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATggtggt                                                                                     >  1:3266789/1‑150 (MQ=255)
                                                                   cggtcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTa                                                                                    >  1:4321513/1‑150 (MQ=255)
                                                                   cggtcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTa                                                                                    >  1:4950922/1‑150 (MQ=255)
                                                                     gtcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAAt                                                                                  <  2:1407721/150‑1 (MQ=255)
                                                                       cggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATag                                                                                <  2:865253/150‑1 (MQ=255)
                                                                       cggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATag                                                                                >  1:3710970/1‑150 (MQ=255)
                                                                        ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATaga                                                                               >  2:726763/1‑150 (MQ=255)
                                                                         gtgtTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                                                              >  1:2813541/1‑150 (MQ=255)
                                                                            ttAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCa                                                                           >  1:2383882/1‑150 (MQ=255)
                                                                                tGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAgaga                                                                       >  2:4458009/1‑150 (MQ=255)
                                                                                 gCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGaa                                                                      <  2:1691404/150‑1 (MQ=255)
                                                                                   cAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGt                                                                    >  2:320231/1‑150 (MQ=255)
                                                                                   cAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGt                                                                    <  2:1517698/150‑1 (MQ=255)
                                                                                    aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGtt                                                                   <  2:3803451/150‑1 (MQ=255)
                                                                                    aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGtt                                                                   >  2:2712728/1‑150 (MQ=255)
                                                                                    aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGtt                                                                   <  2:232445/150‑1 (MQ=255)
                                                                                       cAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTc                                                                <  1:2031688/150‑1 (MQ=255)
                                                                                           cTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTa                                                            >  1:1541239/1‑150 (MQ=255)
                                                                                           cTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTa                                                            <  2:2418689/150‑1 (MQ=255)
                                                                                               ccATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCTAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcc                                                        >  1:4537719/1‑150 (MQ=255)
                                                                                               ccATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcc                                                        <  2:1039678/150‑1 (MQ=255)
                                                                                                 aTTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcccc                                                      <  2:1302967/150‑1 (MQ=255)
                                                                                                 aTTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcccc                                                      <  2:1541931/150‑1 (MQ=255)
                                                                                                 aTTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcccc                                                      <  1:3826353/150‑1 (MQ=255)
                                                                                                    ttCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccacc                                                   >  2:4138373/1‑150 (MQ=255)
                                                                                                    ttCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccacc                                                   <  1:2223795/150‑1 (MQ=255)
                                                                                                     tCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccacca                                                  <  1:65271/150‑1 (MQ=255)
                                                                                                      cAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCcaccac                                                 <  1:2986032/150‑1 (MQ=255)
                                                                                                      cAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCcaccac                                                 <  1:1795855/150‑1 (MQ=255)
                                                                                                      cAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCcaccac                                                 >  1:916268/1‑150 (MQ=255)
                                                                                                       aGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCaccacc                                                >  1:1422289/1‑150 (MQ=255)
                                                                                                            ttCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATCGCGAAGGAAAATCGTCAGCGCCAGCATGGCGAGGACGGTAACAATCCCGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTCTGCCCCACCACCCACTg                                           >  2:3945194/1‑150 (MQ=255)
                                                                                                            ttCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTg                                           >  2:2100943/1‑150 (MQ=255)
                                                                                                             tCCGCGCAGGCACGACACGCGCGACCCATGCGGGGATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGa                                          >  1:4612643/1‑150 (MQ=255)
                                                                                                              ccGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGAc                                         >  1:1000172/1‑150 (MQ=255)
                                                                                                               cgcgCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGAcc                                        <  2:3866136/150‑1 (MQ=255)
                                                                                                                gcgcAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCg                                       <  2:753969/150‑1 (MQ=255)
                                                                                                                gcgcAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCg                                       >  2:3634057/1‑150 (MQ=255)
                                                                                                                      gCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAAc                                 <  1:3475850/150‑1 (MQ=255)
                                                                                                                       cACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACa                                >  1:3620344/1‑150 (MQ=255)
                                                                                                                        aCGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAg                               >  2:4692526/1‑150 (MQ=255)
                                                                                                                         cGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAgg                              >  1:3856708/1‑150 (MQ=255)
                                                                                                                          gACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGc                             <  1:4138373/150‑1 (MQ=255)
                                                                                                                          gACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGc                             >  1:2556499/1‑150 (MQ=255)
                                                                                                                          gACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGc                             >  1:4241532/1‑150 (MQ=255)
                                                                                                                           acacGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCt                            >  2:1174880/1‑150 (MQ=255)
                                                                                                                            cacGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTc                           <  1:1238887/150‑1 (MQ=255)
                                                                                                                                cgcgACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCa                       <  2:1392135/150‑1 (MQ=255)
                                                                                                                                  cgACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAgc                     <  1:4360183/150‑1 (MQ=255)
                                                                                                                                  cgACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAgc                     >  1:2955746/1‑150 (MQ=255)
                                                                                                                                       cATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCAc                >  1:2051758/1‑150 (MQ=255)
                                                                                                                                         tGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGt              >  1:1655393/1‑150 (MQ=255)
                                                                                                                                          gCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTc             <  2:1169272/150‑1 (MQ=255)
                                                                                                                                           cGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcg            >  1:1343848/1‑150 (MQ=255)
                                                                                                                                            gggAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTcgc           >  2:642851/1‑150 (MQ=255)
                                                                                                                                                 tAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGaa      >  1:877812/1‑150 (MQ=255)
                                                                                                                                                     gAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCtt  <  1:973157/150‑1 (MQ=255)
                                                                                                                                                     gAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCtt  >  1:1376504/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAAACAGGCTCGGCAGCGCCACGTCGCGCGAACCTT  >  CP009273/3589089‑3589387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑