BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,598,440	C→A	22.2%	N34K (AAC→AAA)	yhhL →	DUF1145 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,598,440	0	C	A	22.2%	772.2 / 164.7	296	N34K (AAC→AAA)	yhhL	DUF1145 family protein
Reads supporting (aligned to +/- strand): ref base C (133/97); new base A (28/38); total (161/135)
Fisher's exact test for biased strand distribution p-value = 3.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

GTCAGGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAA  >  CP009273/3598291‑3598589

 tCAGGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACg                                                                                                                                                      >  1:3568745/1‑150 (MQ=255)

      tGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGgcg                                                                                                                                                 <  2:2092845/150‑1 (MQ=255)

      tGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGgcg                                                                                                                                                 <  2:138342/150‑1 (MQ=255)

         cTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTg                                                                                                                                              <  2:1000881/150‑1 (MQ=255)

              cGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGAtttt                                                                                                                                         >  1:2619420/1‑150 (MQ=255)

                gCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTa                                                                                                                                       >  2:2528952/1‑150 (MQ=255)

                  tGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAcc                                                                                                                                     <  1:3639955/150‑1 (MQ=255)

                   gTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCg                                                                                                                                    <  1:210954/150‑1 (MQ=255)

                   gTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCg                                                                                                                                    <  1:1903033/150‑1 (MQ=255)

                    tATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGt                                                                                                                                   >  1:658796/1‑150 (MQ=255)

                     aTCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTg                                                                                                                                  <  1:3427682/150‑1 (MQ=255)

                      tCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGc                                                                                                                                 >  2:1163213/1‑150 (MQ=255)

                        aaCGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTg                                                                                                                               <  2:3870936/150‑1 (MQ=255)

                             gAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCa                                                                                                                          >  2:3646765/1‑150 (MQ=255)

                              aaGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCAt                                                                                                                         <  2:1987855/150‑1 (MQ=255)

                              aaGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCAt                                                                                                                         <  2:4157058/150‑1 (MQ=255)

                               aGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATg                                                                                                                        >  1:4766680/1‑150 (MQ=255)

                                  acaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTa                                                                                                                     >  2:3369696/1‑150 (MQ=255)

                                  acaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCAGATGCATGGTa                                                                                                                     >  1:1279293/1‑150 (MQ=255)

                                   caAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTAt                                                                                                                    >  1:3205676/1‑150 (MQ=255)

                                      ggAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCa                                                                                                                 <  1:3982469/150‑1 (MQ=255)

                                      ggAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCa                                                                                                                 <  2:4595700/150‑1 (MQ=255)

                                       gagaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAg                                                                                                                >  2:68117/1‑150 (MQ=255)

                                       gagaAAGTGAGGCTGATAAATATTGGTCTTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCCTGGTATGCAg                                                                                                                >  2:2832248/1‑150 (MQ=255)

                                        agaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGc                                                                                                               >  2:3604820/1‑150 (MQ=255)

                                         gaAAGTGATGCTGTTTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTTGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCt                                                                                                              <  1:1056809/150‑1 (MQ=255)

                                         gaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCt                                                                                                              <  1:3179968/150‑1 (MQ=255)

                                         gaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCAGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCt                                                                                                              >  1:2262370/1‑150 (MQ=255)

                                             gTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGgcg                                                                                                          <  1:1623602/150‑1 (MQ=255)

                                               gATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCt                                                                                                        >  2:4660767/1‑150 (MQ=255)

                                                  gCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTAtt                                                                                                     <  2:3205846/150‑1 (MQ=255)

                                                         aaTATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATcc                                                                                              >  2:424972/1‑150 (MQ=255)

                                                         aaTATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATcc                                                                                              >  2:1400688/1‑150 (MQ=255)

                                                          atatTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCa                                                                                             <  1:762213/150‑1 (MQ=255)

                                                                gTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTAc                                                                                       <  1:4976070/150‑1 (MQ=255)

                                                                   gTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGa                                                                                    <  2:495009/150‑1 (MQ=255)

                                                                    tttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaa                                                                                   <  2:265914/150‑1 (MQ=255)

                                                                    tttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaa                                                                                   >  1:2343095/1‑150 (MQ=255)

                                                                     ttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaaa                                                                                  >  2:4760929/1‑150 (MQ=255)

                                                                     ttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaaa                                                                                  >  1:253506/1‑150 (MQ=255)

                                                                      tGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAg                                                                                 >  1:1937690/1‑150 (MQ=255)

                                                                      tGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAg                                                                                 <  2:2921926/150‑1 (MQ=255)

                                                                        ttAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGAt                                                                               >  1:3793428/1‑150 (MQ=255)

                                                                          aaTGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATgg                                                                             >  1:4004799/1‑150 (MQ=255)

                                                                             gCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGccc                                                                          >  2:1337159/1‑150 (MQ=255)

                                                                                ctGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCa                                                                       >  2:3096959/1‑150 (MQ=255)

                                                                                ctGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCa                                                                       <  1:1330618/150‑1 (MQ=255)

                                                                                 tGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAg                                                                      >  1:4623488/1‑150 (MQ=255)

                                                                                   cGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGAt                                                                    >  2:31747/1‑150 (MQ=255)

                                                                                   cGTTTGGGGAATTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGAt                                                                    <  2:2427223/150‑1 (MQ=255)

                                                                                     tttGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGa                                                                  <  2:4015052/150‑1 (MQ=255)

                                                                                       tGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGacc                                                                >  2:3071447/1‑150 (MQ=255)

                                                                                          ggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccacc                                                             <  1:4434246/150‑1 (MQ=255)

                                                                                             tttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                          <  1:3144805/150‑1 (MQ=255)

                                                                                             tttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                          <  2:2096571/150‑1 (MQ=255)

                                                                                             tttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                          <  2:4690145/150‑1 (MQ=255)

                                                                                              ttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgccg                                                         >  2:5020776/1‑150 (MQ=255)

                                                                                               tttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGa                                                        >  1:735787/1‑150 (MQ=255)

                                                                                               tttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGa                                                        <  2:3306983/150‑1 (MQ=255)

                                                                                                ttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGaa                                                       >  2:757639/1‑150 (MQ=255)

                                                                                                 tAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGaaa                                                      >  1:431381/1‑150 (MQ=255)

                                                                                                 tAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGaaa                                                      >  2:5067615/1‑150 (MQ=255)

                                                                                                        cAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACg                                               <  2:2080751/150‑1 (MQ=255)

                                                                                                          aCCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGa                                             >  1:434380/1‑150 (MQ=255)

                                                                                                           ccTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAt                                            >  1:2934011/1‑150 (MQ=255)

                                                                                                             tGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAttt                                          <  2:3990419/150‑1 (MQ=255)

                                                                                                             tGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAttt                                          >  2:3784757/1‑150 (MQ=255)

                                                                                                               gTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTc                                        <  1:3055719/150‑1 (MQ=255)

                                                                                                                   aTCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACATTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCttt                                    >  1:1276525/1‑150 (MQ=255)

                                                                                                                    tCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCtttt                                   <  1:1400634/150‑1 (MQ=255)

                                                                                                                     cccTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTc                                  <  2:3534771/150‑1 (MQ=255)

                                                                                                                      ccTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCg                                 <  2:2343980/150‑1 (MQ=255)

                                                                                                                      ccTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCg                                 <  1:1463660/150‑1 (MQ=255)

                                                                                                                       cTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCgg                                <  1:2182614/150‑1 (MQ=255)

                                                                                                                        ttCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTCCGGATTTTCCTTTTCGGc                               >  2:1083266/1‑150 (MQ=255)

                                                                                                                        ttCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGc                               >  2:2736046/1‑150 (MQ=255)

                                                                                                                           ccACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtg                            <  2:124360/150‑1 (MQ=255)

                                                                                                                                cccGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGa                       <  1:5120454/150‑1 (MQ=255)

                                                                                                                                 ccGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGaa                      >  2:553290/1‑150 (MQ=255)

                                                                                                                                 ccGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGaa                      >  1:1241692/1‑150 (MQ=255)

                                                                                                                                    cTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActg                   >  1:2765032/1‑150 (MQ=255)

                                                                                                                                    cTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActg                   >  1:4174101/1‑150 (MQ=255)

                                                                                                                                      gAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgct                 <  2:1710009/150‑1 (MQ=255)

                                                                                                                                       aaTATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgctg                <  1:962987/150‑1 (MQ=255)

                                                                                                                                       aaTATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgctg                <  2:3709637/150‑1 (MQ=255)

                                                                                                                                         tatCTTCGTTAACGTGGCGCTGATTTTGACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggc              <  1:1249964/150‑1 (MQ=255)

                                                                                                                                          atCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcc             <  1:5062925/150‑1 (MQ=255)

                                                                                                                                           tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcct            <  1:4112552/150‑1 (MQ=255)

                                                                                                                                            cTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg           >  2:2049127/1‑150 (MQ=255)

                                                                                                                                            cTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg           <  1:4581705/150‑1 (MQ=255)

                                                                                                                                            cTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg           >  2:2592586/1‑150 (MQ=255)

                                                                                                                                              tCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctggc         <  2:1549125/150‑1 (MQ=255)

                                                                                                                                                gTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCGATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCag       <  2:3803246/150‑1 (MQ=255)

                                                                                                                                                 ttAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCaga      >  1:3356454/1‑150 (MQ=255)

                                                                                                                                                  tAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCagaa     <  2:219347/150‑1 (MQ=255)

                                                                                                                                                   aaCGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTGTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCagaag    >  1:368445/1‑150 (MQ=255)

                                                                                                                                                   aaCGTGGCGCTGATTGTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTGTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTCCGGATTTTCCTTGTCGGCGTGTGTGACCTGCTGGCCTGGCcgaag    >  2:4084821/1‑150 (MQ=255)

                                                                                                                                                    aCGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCagaaga   >  1:1007172/1‑150 (MQ=255)

                                                                                                                                                     cGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAgaagaa  >  2:4510103/1‑150 (MQ=255)

                                                                                                                                                     cGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAgaagaa  <  1:1681984/150‑1 (MQ=255)

GTCAGGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAA  >  CP009273/3598291‑3598589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`