Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,679,324 C→A 100% M1070I (ATG→ATT)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,679,3240CA96.9% 1156.3 / 20.8 338M1070I (ATG→ATT) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base A (167/159);  total (170/168)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 346 total aligned reads displayed.

TGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679178‑3679470
                                                                                                                                                  |                                                                                                                                                  
tgGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatc                                                                                                                                                 <  1:3067403/150‑1 (MQ=255)
 ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatca                                                                                                                                                <  2:4797937/150‑1 (MQ=255)
 ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatca                                                                                                                                                <  2:1338413/150‑1 (MQ=255)
   atatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                              <  1:4805608/150‑1 (MQ=255)
   atatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                              <  1:3515424/150‑1 (MQ=255)
   atatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                              <  2:5018355/150‑1 (MQ=255)
       cAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGAt                                                                                                                                          <  2:3301040/150‑1 (MQ=255)
        aaTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATa                                                                                                                                         >  1:4948210/1‑150 (MQ=255)
         aTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATaa                                                                                                                                        >  2:3891928/1‑150 (MQ=255)
                tGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCa                                                                                                                                 <  2:2067321/150‑1 (MQ=255)
                  ccAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATg                                                                                                                               <  1:1495890/150‑1 (MQ=255)
                   caacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATgg                                                                                                                              >  2:1063762/1‑150 (MQ=255)
                    aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGt                                                                                                                             <  2:233140/150‑1 (MQ=255)
                    aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                             <  2:342743/150‑1 (MQ=255)
                     acaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtt                                                                                                                            >  1:102739/1‑150 (MQ=255)
                     acaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtt                                                                                                                            <  1:4648239/150‑1 (MQ=255)
                     acaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtt                                                                                                                            <  2:3152358/150‑1 (MQ=255)
                     acaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtt                                                                                                                            <  2:3946611/150‑1 (MQ=255)
                       aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          >  2:851083/1‑150 (MQ=255)
                          ccAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGt                                                                                                                       >  1:4125460/1‑150 (MQ=255)
                           cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      <  1:3013760/150‑1 (MQ=255)
                             gTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCg                                                                                                                    >  2:3243861/1‑150 (MQ=255)
                               tGGAAGGAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACGGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTg                                                                                                                  <  2:3324481/150‑1 (MQ=255)
                                 gAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                                <  1:3267722/150‑1 (MQ=255)
                                 gAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                                >  1:1146214/1‑150 (MQ=255)
                                  aaGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAAt                                                                                                               >  2:1484986/1‑150 (MQ=255)
                                  aaGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAAt                                                                                                               <  2:4147438/150‑1 (MQ=255)
                                  aaGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAAt                                                                                                               <  2:5139868/150‑1 (MQ=255)
                                   aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                              <  1:844584/150‑1 (MQ=255)
                                        cacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGAcc                                                                                                         >  1:751616/1‑150 (MQ=255)
                                          cgtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCAc                                                                                                       <  2:550305/150‑1 (MQ=255)
                                           gtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACg                                                                                                      >  2:3629676/1‑150 (MQ=255)
                                              gtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCGGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGc                                                                                                   <  2:4711547/150‑1 (MQ=255)
                                              gtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGc                                                                                                   >  1:2607990/1‑150 (MQ=255)
                                                tCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCa                                                                                                 >  1:4252514/1‑150 (MQ=255)
                                                   aGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGAc                                                                                              <  1:3997244/150‑1 (MQ=255)
                                                    gTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACg                                                                                             <  2:4013797/150‑1 (MQ=255)
                                                         gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAccc                                                                                        <  2:2257308/150‑1 (MQ=255)
                                                         gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAccc                                                                                        <  2:3032169/150‑1 (MQ=255)
                                                         gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAccc                                                                                        >  1:2118616/1‑150 (MQ=255)
                                                          cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       <  1:4615408/150‑1 (MQ=255)
                                                          cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       >  1:2478529/1‑150 (MQ=255)
                                                          cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       >  1:3972714/1‑150 (MQ=255)
                                                           ccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAg                                                                                      <  1:1316678/150‑1 (MQ=255)
                                                            cgcgCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGc                                                                                     >  1:151053/1‑150 (MQ=255)
                                                            cgcgCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGc                                                                                     >  2:669604/1‑150 (MQ=255)
                                                               gcCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAGTCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTcc                                                                                  >  1:1736645/1‑150 (MQ=255)
                                                                  gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               >  2:2084264/1‑150 (MQ=255)
                                                                  gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               <  1:2200497/150‑1 (MQ=255)
                                                                  gtggAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               <  1:4163979/150‑1 (MQ=255)
                                                                     tAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAc                                                                            >  1:4784856/1‑150 (MQ=255)
                                                                         cGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGt                                                                        >  1:552434/1‑150 (MQ=255)
                                                                          gAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                       >  2:2008935/1‑150 (MQ=255)
                                                                              cccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTcc                                                                   >  2:2575023/1‑150 (MQ=255)
                                                                               ccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCg                                                                  >  2:74443/1‑150 (MQ=255)
                                                                                cTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                                 <  2:2732883/150‑1 (MQ=255)
                                                                                cTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGt                                                                 <  2:1626959/150‑1 (MQ=255)
                                                                                  gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgc                                                               >  2:3868344/1‑150 (MQ=255)
                                                                                  gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgc                                                               <  2:3676863/150‑1 (MQ=255)
                                                                                     tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCt                                                            <  2:3571250/150‑1 (MQ=255)
                                                                                        tgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTccc                                                         <  1:4606311/150‑1 (MQ=255)
                                                                                              cATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcaca                                                   >  1:3437361/1‑150 (MQ=255)
                                                                                                tCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATc                                                 <  1:1931128/150‑1 (MQ=255)
                                                                                                  gTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAc                                               <  1:2882396/150‑1 (MQ=255)
                                                                                                  gTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAc                                               >  1:4413399/1‑150 (MQ=255)
                                                                                                  gTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAc                                               >  1:4373854/1‑150 (MQ=255)
                                                                                                   ttGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAcc                                              <  1:1020875/150‑1 (MQ=255)
                                                                                                   ttGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAcc                                              <  1:4563599/150‑1 (MQ=255)
                                                                                                    tGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGCTAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCg                                             <  2:4317411/150‑1 (MQ=255)
                                                                                                      gATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGt                                           >  2:2852720/1‑150 (MQ=255)
                                                                                                                 aGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTCTGGCAAACGa                                >  1:903850/1‑150 (MQ=255)
                                                                                                                  gCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGAc                               <  2:884496/150‑1 (MQ=255)
                                                                                                                   cttcttCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACa                              >  1:365903/1‑150 (MQ=255)
                                                                                                                    ttcttcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAg                             >  2:3008557/1‑150 (MQ=255)
                                                                                                                    ttcttcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAg                             >  2:4394647/1‑150 (MQ=255)
                                                                                                                      cttcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt                           <  2:1718000/150‑1 (MQ=255)
                                                                                                                       ttcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTa                          >  2:4926890/1‑150 (MQ=255)
                                                                                                                        tcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAt                         >  1:2975623/1‑150 (MQ=255)
                                                                                                                        tcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAt                         <  1:3825757/150‑1 (MQ=255)
                                                                                                                         ccggCCAGTGGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCTGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAtt                        <  1:2360527/147‑1 (MQ=255)
                                                                                                                         ccggCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAtt                        <  1:5086117/147‑1 (MQ=255)
                                                                                                                           ggCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTcc                      >  1:1580176/2‑150 (MQ=255)
                                                                                                                            gCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCt                     <  1:851083/150‑1 (MQ=255)
                                                                                                                            gCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCt                     <  2:2644242/150‑1 (MQ=255)
                                                                                                                                gtcggtcgGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCgg                 <  1:967579/150‑1 (MQ=255)
                                                                                                                                   ggtcggGATCATATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACt              <  1:674943/150‑1 (MQ=255)
                                                                                                                                    gtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTg             >  1:188001/1‑150 (MQ=255)
                                                                                                                                      cggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgta           >  1:5135787/1‑150 (MQ=255)
                                                                                                                                       gggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtag          <  1:3414722/150‑1 (MQ=255)
                                                                                                                                         gATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagta        >  1:3143182/1‑150 (MQ=255)
                                                                                                                                         gATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagta        >  1:4218533/1‑150 (MQ=255)
                                                                                                                                            cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACTAGCCAGACTCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGAAAGGTATTCCTGCGGACTGTAGTAAcc     >  2:1104351/1‑150 (MQ=255)
                                                                                                                                            cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  2:4255165/150‑1 (MQ=255)
                                                                                                                                            cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  1:2545726/150‑1 (MQ=255)
                                                                                                                                            cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  1:1060433/150‑1 (MQ=255)
                                                                                                                                            cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAAGGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCACACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  2:1237650/150‑1 (MQ=255)
                                                                                                                                             aGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCg    >  2:1812663/1‑150 (MQ=255)
                                                                                                                                             aGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCg    >  1:4897635/1‑150 (MQ=255)
                                                                                                                                               aTTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGcc  >  1:2804640/1‑150 (MQ=255)
                                                                                                                                               aTTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGcc  >  1:4958787/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679178‑3679470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑