Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,500,138 G→T 35.8% I186I (ATC→ATA)  yhfT ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,500,1380GT35.8% 357.3 / 250.0 257I186I (ATC→ATA) yhfTinner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (72/93);  new base T (30/62);  total (102/155)
Fisher's exact test for biased strand distribution p-value = 8.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

Only 100 of 257 total aligned reads displayed.

CCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAACCA  >  CP009273/3499990‑3500286
                                                                                                                                                    |                                                                                                                                                    
ccACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGa                                                                                                                                                     <  1:3784131/150‑1 (MQ=255)
ccACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGa                                                                                                                                                     >  1:880267/1‑150 (MQ=255)
   cGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGAttt                                                                                                                                                  <  2:1016571/150‑1 (MQ=255)
    gATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTc                                                                                                                                                 <  1:157561/150‑1 (MQ=255)
     aTGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCg                                                                                                                                                >  1:2201473/1‑150 (MQ=255)
      tGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGa                                                                                                                                               >  1:1099159/1‑150 (MQ=255)
      tGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCACGCCAATAAAGATTTCGa                                                                                                                                               >  1:3375680/1‑150 (MQ=255)
        gCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATg                                                                                                                                             <  2:668414/150‑1 (MQ=255)
        gCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATg                                                                                                                                             <  2:1619436/150‑1 (MQ=255)
          gATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGa                                                                                                                                           <  1:1930056/150‑1 (MQ=255)
           atatAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGAt                                                                                                                                          >  1:3290628/1‑150 (MQ=255)
               aGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAg                                                                                                                                      <  2:1277989/150‑1 (MQ=255)
                gggTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAgg                                                                                                                                     <  2:1304526/150‑1 (MQ=255)
                 ggTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAggg                                                                                                                                    <  1:1726327/150‑1 (MQ=255)
                   tAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGtt                                                                                                                                  <  1:4149819/150‑1 (MQ=255)
                    aaGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTa                                                                                                                                 <  2:2717572/150‑1 (MQ=255)
                     aGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTaa                                                                                                                                >  2:4022974/1‑150 (MQ=255)
                      gTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAg                                                                                                                               <  1:1298100/150‑1 (MQ=255)
                       tttttGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGa                                                                                                                              >  2:3188627/1‑150 (MQ=255)
                       tttttGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGa                                                                                                                              >  2:3141048/1‑150 (MQ=255)
                       tttttGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGa                                                                                                                              >  2:3141057/1‑150 (MQ=255)
                        ttttGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGAt                                                                                                                             <  2:1546785/150‑1 (MQ=255)
                         tttGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGAtg                                                                                                                            <  1:1779446/150‑1 (MQ=255)
                           tGATAATCCGTGACGTGCGTTCTTCAAACACCGAACTCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGAtttg                                                                                                                          <  2:4009121/150‑1 (MQ=255)
                             aTAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTggg                                                                                                                        >  2:820017/1‑150 (MQ=255)
                             aTAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTggg                                                                                                                        <  1:2662676/150‑1 (MQ=255)
                                aTCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGaaa                                                                                                                     >  1:2079386/1‑150 (MQ=255)
                                 tCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAAt                                                                                                                    >  2:1117047/1‑150 (MQ=255)
                                 tCCGTGACGTGCGTTCTACAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAAt                                                                                                                    <  2:3226893/150‑1 (MQ=255)
                                      gACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAgcgc                                                                                                               >  1:1832489/1‑150 (MQ=255)
                                       aCGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCa                                                                                                              <  1:4507942/150‑1 (MQ=255)
                                               tcttcAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTAcc                                                                                                      <  1:4274282/150‑1 (MQ=255)
                                               tcttcAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTAcc                                                                                                      <  1:4274254/150‑1 (MQ=255)
                                                 ttcAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACcac                                                                                                    <  1:3482933/150‑1 (MQ=255)
                                                  tcAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACcaca                                                                                                   >  2:147710/1‑150 (MQ=255)
                                                  tcAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACcaca                                                                                                   <  2:286981/150‑1 (MQ=255)
                                                   cAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCacac                                                                                                  <  1:4051902/150‑1 (MQ=255)
                                                     aaCACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACgg                                                                                                <  1:3513079/150‑1 (MQ=255)
                                                       cacCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGt                                                                                              >  1:360377/1‑150 (MQ=255)
                                                        acCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGtc                                                                                             <  2:2198234/150‑1 (MQ=255)
                                                           gAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGtcatc                                                                                          <  1:4070646/150‑1 (MQ=255)
                                                           gAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGtcatc                                                                                          <  1:4193244/150‑1 (MQ=255)
                                                           gAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGtcatc                                                                                          <  1:1087856/150‑1 (MQ=255)
                                                             aaGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAg                                                                                        <  1:2360590/150‑1 (MQ=255)
                                                              aGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGt                                                                                       <  1:2767562/150‑1 (MQ=255)
                                                              aGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGt                                                                                       <  1:641211/150‑1 (MQ=255)
                                                              aGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGt                                                                                       <  1:793139/150‑1 (MQ=255)
                                                                 ccGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTacc                                                                                    <  2:2506123/150‑1 (MQ=255)
                                                                 ccGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTacc                                                                                    <  2:2506448/150‑1 (MQ=255)
                                                                    cTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTaccacc                                                                                 <  2:4491791/150‑1 (MQ=255)
                                                                     tGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTAccacca                                                                                <  2:3888886/150‑1 (MQ=255)
                                                                     tGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTAccacca                                                                                >  1:2983169/1‑150 (MQ=255)
                                                                     tGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTAccacca                                                                                <  1:2427684/150‑1 (MQ=255)
                                                                     tGGCATCAATGTCATGTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTAccacca                                                                                <  1:2427790/150‑1 (MQ=255)
                                                                          tCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCAcggcg                                                                           >  1:2428966/1‑150 (MQ=255)
                                                                          tCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCAcggcg                                                                           <  2:4434664/150‑1 (MQ=255)
                                                                             aTGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGgcggcg                                                                        >  2:1748670/1‑150 (MQ=255)
                                                                              tGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGACATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGa                                                                       >  1:1427422/1‑150 (MQ=255)
                                                                               gTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGAt                                                                      >  2:1731950/1‑150 (MQ=255)
                                                                                      tcatcaCGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACtt                                                               <  1:400520/150‑1 (MQ=255)
                                                                                         tcaCGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGc                                                            >  1:476321/1‑150 (MQ=255)
                                                                                             gATGACGCAGGTCGTGAGTGATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTcc                                                        <  2:186211/150‑1 (MQ=255)
                                                                                              aTGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCa                                                       >  1:3231642/1‑150 (MQ=255)
                                                                                              aTGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCa                                                       >  1:3231880/1‑150 (MQ=255)
                                                                                                gACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGc                                                     >  2:1674478/1‑150 (MQ=255)
                                                                                                 aCGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGcc                                                    >  1:752238/1‑150 (MQ=255)
                                                                                                 aCGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGcc                                                    <  1:1349907/150‑1 (MQ=255)
                                                                                                   gCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCaa                                                  <  1:1719979/150‑1 (MQ=255)
                                                                                                     aGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAAc                                                >  1:3124901/1‑150 (MQ=255)
                                                                                                      ggTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGAGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACt                                               >  2:73028/1‑150 (MQ=255)
                                                                                                         cGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGt                                            >  2:1372147/1‑150 (MQ=255)
                                                                                                          gTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTa                                           <  2:1778202/150‑1 (MQ=255)
                                                                                                            gAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGa                                         <  1:3031724/150‑1 (MQ=255)
                                                                                                             aGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGaa                                        <  2:211606/150‑1 (MQ=255)
                                                                                                                tATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAAt                                     <  2:2081841/150‑1 (MQ=255)
                                                                                                                 aTCGCGATCCCCAGCAGCATCACCATGACAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATc                                    <  1:1381287/150‑1 (MQ=255)
                                                                                                                    gcgATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGcc                                 >  1:4461309/1‑150 (MQ=255)
                                                                                                                     cgATCCCCAGCAGCATCACCATGCCAATACATAGTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGcca                                <  2:3007003/150‑1 (MQ=255)
                                                                                                                      gATCCCCAGCAGCATCACCATGCCAATACATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGccac                               <  2:2635636/150‑1 (MQ=255)
                                                                                                                           ccAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCg                          >  2:2279267/1‑150 (MQ=255)
                                                                                                                           ccAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCg                          <  2:4186822/150‑1 (MQ=255)
                                                                                                                            cagcagCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCgg                         >  1:2356221/1‑150 (MQ=255)
                                                                                                                            cagcagCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCCCCAGCgg                         >  2:4297669/1‑150 (MQ=255)
                                                                                                                             agcagcATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCggg                        <  2:2512879/150‑1 (MQ=255)
                                                                                                                               cagcaTCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGaa                      <  2:1489866/150‑1 (MQ=255)
                                                                                                                               cagcaTCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGaa                      <  2:2181268/150‑1 (MQ=255)
                                                                                                                                agcaTCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAAc                     >  2:3496361/1‑150 (MQ=255)
                                                                                                                                       ccATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCaa              <  2:1722338/150‑1 (MQ=255)
                                                                                                                                         aTGCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCaaaa            <  1:4033409/150‑1 (MQ=255)
                                                                                                                                         aTGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCaaaa            <  2:1890267/150‑1 (MQ=255)
                                                                                                                                         aTGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCaaaa            <  1:1674478/150‑1 (MQ=255)
                                                                                                                                         aTGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACAACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCaaaa            >  2:1629553/1‑150 (MQ=255)
                                                                                                                                          tGCCAATAAATAATTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAg           <  1:2603862/150‑1 (MQ=255)
                                                                                                                                           gCCAATAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGc          <  1:2280765/150‑1 (MQ=255)
                                                                                                                                             cAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTg        >  1:1084276/1‑150 (MQ=255)
                                                                                                                                               aTAAATATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGaa      >  1:2558987/1‑150 (MQ=255)
                                                                                                                                                tAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGaaa     <  1:1229107/150‑1 (MQ=255)
                                                                                                                                                 aaaGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAac    <  2:1446754/150‑1 (MQ=255)
                                                                                                                                                 aaaGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAac    <  2:269350/150‑1 (MQ=255)
                                                                                                                                                  aaGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAacc   <  2:3993655/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAACCA  >  CP009273/3499990‑3500286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑