Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 309,785 T→A 10.7% intergenic (‑269/‑283) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273309,7850TA10.7% 786.5 / 63.5 251intergenic (‑269/‑283)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base T (100/124);  new base A (16/11);  total (116/135)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGG  >  CP009273/309636‑309933
                                                                                                                                                     |                                                                                                                                                    
tCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAatt                                                                                                                                                      <  1:69827/150‑1 (MQ=255)
tCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAatt                                                                                                                                                      >  2:2841290/1‑150 (MQ=255)
  gATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattat                                                                                                                                                    <  1:3585752/150‑1 (MQ=255)
  gATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattat                                                                                                                                                    <  2:263167/150‑1 (MQ=255)
  gATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattat                                                                                                                                                    <  1:295522/150‑1 (MQ=255)
   atatCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAattatt                                                                                                                                                   >  1:4398237/1‑150 (MQ=255)
     atCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCa                                                                                                                                                 <  1:1191342/150‑1 (MQ=255)
     atCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATAGACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCa                                                                                                                                                 <  1:3464233/150‑1 (MQ=255)
        tcCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTg                                                                                                                                              <  1:1231702/150‑1 (MQ=255)
         ccGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGt                                                                                                                                             >  1:3820720/1‑150 (MQ=255)
             aaTTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGc                                                                                                                                         <  2:688863/150‑1 (MQ=255)
               tttACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCag                                                                                                                                       >  2:1005689/1‑150 (MQ=255)
                ttACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCaga                                                                                                                                      >  2:1709361/1‑150 (MQ=255)
                 tACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCagag                                                                                                                                     <  2:1989909/150‑1 (MQ=255)
                  aCAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGt                                                                                                                                    <  2:660949/150‑1 (MQ=255)
                    aGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTaa                                                                                                                                  <  2:288538/150‑1 (MQ=255)
                    aGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTaa                                                                                                                                  >  2:952529/1‑150 (MQ=255)
                      tCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGa                                                                                                                                >  2:278155/1‑150 (MQ=255)
                      tCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGa                                                                                                                                >  2:278169/1‑150 (MQ=255)
                        cGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGaaa                                                                                                                              <  1:4354360/150‑1 (MQ=255)
                        cGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGaaa                                                                                                                              >  2:3055425/1‑150 (MQ=255)
                           tGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAatat                                                                                                                           >  1:3897496/1‑150 (MQ=255)
                           tGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAatat                                                                                                                           <  1:807345/150‑1 (MQ=255)
                            gAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAAtata                                                                                                                          >  1:3378525/1‑150 (MQ=255)
                             aaGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATaa                                                                                                                         >  2:3067374/1‑150 (MQ=255)
                              aGGAAAATGAAAATCCATAAAAAATATATTGGTTAATACTGGAATATATACATATTTCGACGAATTTCCAATAGTTTTAATTAAAGGCAGGTCATTTATATTCACTCTTAATAGTGAATTAATAACTGTCCGCAGAGAAAGAAAAATAAc                                                                                                                        >  2:2987056/1‑150 (MQ=255)
                                gAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACtt                                                                                                                      >  1:2382804/1‑150 (MQ=255)
                                gAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACtt                                                                                                                      >  1:2043598/1‑150 (MQ=255)
                                 aaaaTGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTa                                                                                                                     >  2:2335039/1‑150 (MQ=255)
                                  aaaTGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAg                                                                                                                    <  1:2300386/150‑1 (MQ=255)
                                    aTGAATATCCATAAAAAGTATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGt                                                                                                                  <  2:814043/150‑1 (MQ=255)
                                    aTGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGt                                                                                                                  >  1:3452063/1‑150 (MQ=255)
                                      gAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTAt                                                                                                                <  2:3277110/150‑1 (MQ=255)
                                       aaTATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATc                                                                                                               <  1:4093129/150‑1 (MQ=255)
                                        atatCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCt                                                                                                              <  2:2494805/150‑1 (MQ=255)
                                         tatCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTa                                                                                                             >  1:117352/1‑150 (MQ=255)
                                          atCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTAt                                                                                                            >  2:4227661/1‑150 (MQ=255)
                                            ccATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTAttt                                                                                                          >  1:974480/1‑150 (MQ=255)
                                             cATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTa                                                                                                         <  1:3663615/150‑1 (MQ=255)
                                               tAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAAt                                                                                                       <  2:3514159/150‑1 (MQ=255)
                                                aaaaaaTATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAGTATAACTTAGGTATCTATTTAATg                                                                                                      >  2:303644/1‑150 (MQ=255)
                                                 aaaaaTATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGa                                                                                                     <  1:4435620/150‑1 (MQ=255)
                                                   aaaTATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACt                                                                                                   <  2:4508387/150‑1 (MQ=255)
                                                    aaTATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACtt                                                                                                  <  1:1442155/150‑1 (MQ=255)
                                                     atatatTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTg                                                                                                 <  2:4302054/150‑1 (MQ=255)
                                                       atatTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGca                                                                                               <  2:3724694/150‑1 (MQ=255)
                                                         atTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGcaca                                                                                             <  2:3798251/150‑1 (MQ=255)
                                                          ttGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACaa                                                                                            >  1:687826/1‑150 (MQ=255)
                                                                tATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCt                                                                                      >  1:2240943/1‑150 (MQ=255)
                                                                tATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCt                                                                                      >  2:939553/1‑150 (MQ=255)
                                                                 aTCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTa                                                                                     <  1:2693039/150‑1 (MQ=255)
                                                                  tCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTaa                                                                                    >  1:1552316/1‑150 (MQ=255)
                                                                     tGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAAtt                                                                                 <  2:2438313/150‑1 (MQ=255)
                                                                       gCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCATGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAAtttt                                                                               <  2:483054/150‑1 (MQ=255)
                                                                         atatatACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTcc                                                                             >  1:82780/1‑150 (MQ=255)
                                                                         atatatACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTcc                                                                             <  1:4536463/150‑1 (MQ=255)
                                                                            tataCCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTccccc                                                                          >  1:2904550/1‑150 (MQ=255)
                                                                              taCCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCat                                                                        <  2:1674718/150‑1 (MQ=255)
                                                                              taCCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCat                                                                        <  1:1662474/150‑1 (MQ=255)
                                                                                ccTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCataa                                                                      <  1:1366185/150‑1 (MQ=255)
                                                                                ccTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCataa                                                                      <  1:644484/150‑1 (MQ=255)
                                                                                  tATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCataaat                                                                    >  2:615661/1‑150 (MQ=255)
                                                                                     ttCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCAtaaataaa                                                                 >  2:4382495/1‑150 (MQ=255)
                                                                                      tCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATaaaa                                                                >  1:4013705/1‑150 (MQ=255)
                                                                                         acttATTTACAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAata                                                             <  2:2961857/147‑1 (MQ=255)
                                                                                         aCGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAata                                                             >  2:2270488/1‑150 (MQ=255)
                                                                                         aCGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAata                                                             >  2:3136879/1‑150 (MQ=255)
                                                                                         aCGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAata                                                             <  2:3849368/150‑1 (MQ=255)
                                                                                          cGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAatat                                                            >  1:3131931/1‑150 (MQ=255)
                                                                                           gTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAAtata                                                           >  1:1522242/1‑150 (MQ=255)
                                                                                            tATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATaa                                                          >  1:1002999/1‑150 (MQ=255)
                                                                                            tATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATaa                                                          >  2:3538009/1‑150 (MQ=255)
                                                                                             aTTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAAt                                                         <  2:2805246/150‑1 (MQ=255)
                                                                                               ttCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATcc                                                       >  1:2606191/1‑150 (MQ=255)
                                                                                                 ccAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCcg                                                     <  2:3483272/150‑1 (MQ=255)
                                                                                                 ccAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCcg                                                     >  1:1544140/1‑150 (MQ=255)
                                                                                                  cAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCcgc                                                    <  2:1476974/150‑1 (MQ=255)
                                                                                                  cAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCcgc                                                    <  2:1069348/150‑1 (MQ=255)
                                                                                                   aaTAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCcgcg                                                   >  2:3617024/1‑150 (MQ=255)
                                                                                                        ttttAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCaa                                              >  2:980550/1‑150 (MQ=255)
                                                                                                           taattaaAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATAATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAacca                                           >  1:3128444/1‑150 (MQ=255)
                                                                                                               taaAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtg                                       >  2:2199736/1‑150 (MQ=255)
                                                                                                               taaAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtg                                       >  2:4438815/1‑150 (MQ=255)
                                                                                                                 aaGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtgat                                     <  2:3378535/150‑1 (MQ=255)
                                                                                                                 aaGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtgat                                     <  1:3757771/150‑1 (MQ=255)
                                                                                                                 aaGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtgat                                     <  2:1179488/150‑1 (MQ=255)
                                                                                                                 aaGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAAGAAAAATATAATCCCGCGCCCAACCACCtgat                                     >  2:4072478/1‑150 (MQ=255)
                                                                                                                  aGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGATTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCtgatg                                    <  1:867409/150‑1 (MQ=255)
                                                                                                                     catgTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGt                                 <  2:173635/147‑1 (MQ=255)
                                                                                                                     cAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGt                                 >  2:341647/1‑150 (MQ=255)
                                                                                                                               ttATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGc                       >  2:1344645/1‑150 (MQ=255)
                                                                                                                                 aTTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCAc                     <  2:4109956/150‑1 (MQ=255)
                                                                                                                                    cACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTgg                  >  1:957901/1‑150 (MQ=255)
                                                                                                                                    cACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTgg                  >  2:3230743/1‑150 (MQ=255)
                                                                                                                                      ctctGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGAGGAGTGGCGATAGGCACTGGat                >  1:3397830/1‑150 (MQ=255)
                                                                                                                                           aaTAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATAtt           >  2:1755140/1‑150 (MQ=255)
                                                                                                                                           aaTAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATAtt           >  1:1758099/1‑150 (MQ=255)
                                                                                                                                             tAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAg         >  1:2279156/1‑150 (MQ=255)
                                                                                                                                                tGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGGTATATTAGGTg      <  2:1552103/150‑1 (MQ=255)
                                                                                                                                                tGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTg      <  2:2014625/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGATATCTCCGCAATTTACAGTCCGATGAAGGAAAATGAATATCCATAAAAAATATATTGGTTTATCCTGGCATATATACCTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGG  >  CP009273/309636‑309933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑