Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,507,749 G→T 88.0% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,507,7490GT88.0% 667.1 / 85.7 257H224N (CAT→AAT) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (24/7);  new base T (122/104);  total (146/111)
Fisher's exact test for biased strand distribution p-value = 1.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507602‑3507895
                                                                                                                                                   |                                                                                                                                                  
ccAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTAc                                                                                                                                                  <  1:3046462/150‑1 (MQ=255)
    gTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCAttacttac                                                                                                                                              >  1:4454119/1‑150 (MQ=255)
    gTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTAc                                                                                                                                              <  1:4108277/150‑1 (MQ=255)
           cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       >  1:3769910/1‑150 (MQ=255)
            gcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGAAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCa                                                                                                                                      <  1:772875/150‑1 (MQ=255)
              gTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGt                                                                                                                                    >  1:3271086/1‑150 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 <  2:2323369/150‑1 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 >  1:9981/1‑150 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCa                                                                                                                                 >  2:4187530/1‑150 (MQ=255)
                  ttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCAc                                                                                                                                >  2:754029/1‑150 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               <  1:1269774/150‑1 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               <  1:1124571/150‑1 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               >  1:1912881/1‑150 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               >  2:3933827/1‑150 (MQ=255)
                    cACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTg                                                                                                                              <  2:1784540/150‑1 (MQ=255)
                     aCCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCTAGATCAATAGCGACGCCGCGAATATCTTCTAACTTATTCATTACTTACCTTTGCCAGTTCACTgc                                                                                                                             >  2:2633033/1‑150 (MQ=255)
                     aCCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgc                                                                                                                             >  1:2186236/1‑150 (MQ=255)
                        tGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCa                                                                                                                          >  2:1904701/1‑150 (MQ=255)
                        tGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCa                                                                                                                          >  2:3781278/1‑150 (MQ=255)
                         gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         >  1:2070012/1‑150 (MQ=255)
                         gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         >  1:224761/1‑150 (MQ=255)
                         gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         >  1:2523788/1‑150 (MQ=255)
                          cGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAtt                                                                                                                        >  1:2479189/1‑150 (MQ=255)
                          cGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCAtt                                                                                                                        >  2:2062345/1‑150 (MQ=255)
                           gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:3599179/150‑1 (MQ=255)
                              gggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcat                                                                                                                    >  2:1437117/1‑150 (MQ=255)
                              gggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTtcat                                                                                                                    >  1:3817034/1‑150 (MQ=255)
                                 cAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCaa                                                                                                                 >  2:3941878/1‑150 (MQ=255)
                                   aCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATg                                                                                                               <  2:2264602/150‑1 (MQ=255)
                                       cAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtt                                                                                                           >  2:3785482/1‑150 (MQ=255)
                                       cAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGAAtt                                                                                                           <  1:2463238/150‑1 (MQ=255)
                                             aTACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGt                                                                                                     >  2:4150652/1‑150 (MQ=255)
                                              tACAGCGCCATATCTACGGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTa                                                                                                    <  2:1806048/150‑1 (MQ=255)
                                              tACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTa                                                                                                    <  1:2773210/150‑1 (MQ=255)
                                                cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGt                                                                                                  <  1:1620823/150‑1 (MQ=255)
                                                cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGt                                                                                                  <  1:2525918/150‑1 (MQ=255)
                                                cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGt                                                                                                  <  1:1620786/150‑1 (MQ=255)
                                                  gcgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                                                                <  2:2359865/150‑1 (MQ=255)
                                                   cgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctg                                                                                               >  1:2925932/1‑150 (MQ=255)
                                                     ccATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggc                                                                                             <  2:1660905/150‑1 (MQ=255)
                                                      cATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggct                                                                                            <  1:857448/150‑1 (MQ=255)
                                                      cATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggct                                                                                            <  2:4435799/150‑1 (MQ=255)
                                                       atatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                           >  1:2017146/1‑150 (MQ=255)
                                                        tatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctgg                                                                                          >  2:599768/1‑150 (MQ=255)
                                                        tatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctgg                                                                                          >  2:600224/1‑150 (MQ=255)
                                                           cTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCg                                                                                       >  1:1957307/1‑150 (MQ=255)
                                                            tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGa                                                                                      >  1:3101923/1‑150 (MQ=255)
                                                               cGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGa                                                                                   <  2:537209/150‑1 (MQ=255)
                                                                gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                  <  1:3299499/150‑1 (MQ=255)
                                                                gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                  >  1:690019/1‑150 (MQ=255)
                                                                   gcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGc                                                                               <  1:1202957/150‑1 (MQ=255)
                                                                   gcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGc                                                                               <  1:3475424/150‑1 (MQ=255)
                                                                        aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                          <  1:395627/150‑1 (MQ=255)
                                                                        aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                          >  2:1855603/1‑150 (MQ=255)
                                                                        aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAATTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                          >  2:3717809/1‑150 (MQ=255)
                                                                         aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAcc                                                                         <  2:1262958/150‑1 (MQ=255)
                                                                          gACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCg                                                                        >  2:3991477/1‑150 (MQ=255)
                                                                          gACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCg                                                                        >  2:1316248/1‑150 (MQ=255)
                                                                          gACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCg                                                                        >  1:3491299/1‑150 (MQ=255)
                                                                           aCCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCgg                                                                       >  1:2623733/1‑150 (MQ=255)
                                                                             cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                     >  1:4192593/1‑150 (MQ=255)
                                                                             cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                     >  1:4192661/1‑150 (MQ=255)
                                                                                           aCCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcgc                                                       >  2:2936467/1‑150 (MQ=255)
                                                                                            ccAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcgcg                                                      <  1:1256993/150‑1 (MQ=255)
                                                                                            ccAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcgcg                                                      <  1:1758491/150‑1 (MQ=255)
                                                                                             cAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCgcgc                                                     <  1:3280636/150‑1 (MQ=255)
                                                                                             cAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCgcgc                                                     >  2:4196000/1‑150 (MQ=255)
                                                                                              aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCAATGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    >  2:2181472/1‑150 (MQ=255)
                                                                                                  tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                >  2:337675/1‑150 (MQ=255)
                                                                                                   aCCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCctgccg                                               >  1:1001539/1‑150 (MQ=255)
                                                                                                   aCCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCccgccg                                               >  1:576906/1‑150 (MQ=255)
                                                                                                   aCCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCccgccg                                               >  1:1001331/1‑150 (MQ=255)
                                                                                                     cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCa                                             >  2:1504803/1‑150 (MQ=255)
                                                                                                      aTCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAg                                            <  2:3115331/150‑1 (MQ=255)
                                                                                                         aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                         <  1:426593/150‑1 (MQ=255)
                                                                                                             tCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTc                                     >  2:1080035/1‑150 (MQ=255)
                                                                                                               aaaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGc                                   >  1:1816520/1‑150 (MQ=255)
                                                                                                                 aaGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACGGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCa                                 >  2:1935640/1‑150 (MQ=255)
                                                                                                                   gCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAt                               >  2:1232844/1‑150 (MQ=255)
                                                                                                                     gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                             >  2:1328005/1‑150 (MQ=255)
                                                                                                                         ccgcGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATgttgtt                         >  2:759641/1‑150 (MQ=255)
                                                                                                                          cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                        >  1:1742390/1‑150 (MQ=255)
                                                                                                                          cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                        >  2:4397745/1‑150 (MQ=255)
                                                                                                                             gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  1:746967/1‑150 (MQ=255)
                                                                                                                             gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  1:4085377/1‑150 (MQ=255)
                                                                                                                              aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                    <  1:867101/150‑1 (MQ=255)
                                                                                                                                 atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 <  1:1956487/150‑1 (MQ=255)
                                                                                                                                 atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 >  2:865199/1‑150 (MQ=255)
                                                                                                                                   cTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACg               <  1:1776414/150‑1 (MQ=255)
                                                                                                                                   cTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACg               >  2:163851/1‑150 (MQ=255)
                                                                                                                                      cAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCa            <  1:3718320/150‑1 (MQ=255)
                                                                                                                                      cAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCa            <  2:3817034/150‑1 (MQ=255)
                                                                                                                                       aaaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAc           >  2:4021352/1‑150 (MQ=255)
                                                                                                                                       aaaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAc           >  2:206021/1‑150 (MQ=255)
                                                                                                                                         aCTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCAAGCCACCg         >  1:4411492/1‑150 (MQ=255)
                                                                                                                                          cTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGt        <  1:3658746/150‑1 (MQ=255)
                                                                                                                                          cTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGt        >  2:110641/1‑150 (MQ=255)
                                                                                                                                             aTTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCa     >  2:15239/1‑150 (MQ=255)
                                                                                                                                                cATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACtt  <  2:3540420/150‑1 (MQ=255)
                                                                                                                                                cATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACtt  >  1:1097676/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507602‑3507895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑