BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,523,740	T→A	6.1%	L203* (TTA→TAA)	hslO →	heat shock protein Hsp33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,523,740	0	T	A	6.1%	795.4 / 32.0	248	L203* (TTA→TAA)	hslO	heat shock protein Hsp33
Reads supporting (aligned to +/- strand): ref base T (104/128); new base A (7/8); total (111/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 251 total aligned reads displayed.

CCTGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGATAGCATCC  >  CP009273/3523591‑3523889

 cTGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTa                                                                                                                                                      >  2:1356720/1‑150 (MQ=255)

  tGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTAc                                                                                                                                                     <  1:2663799/150‑1 (MQ=255)

    tttATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCg                                                                                                                                                   >  2:1254146/1‑150 (MQ=255)

    tttATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCg                                                                                                                                                   >  1:174553/1‑150 (MQ=255)

    tttATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCg                                                                                                                                                   >  1:2474705/1‑150 (MQ=255)

     ttATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCgg                                                                                                                                                  <  2:3052831/150‑1 (MQ=255)

     ttATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTGTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCgg                                                                                                                                                  >  1:1020472/1‑150 (MQ=255)

         tCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCaaa                                                                                                                                              <  1:1059088/150‑1 (MQ=255)

         tCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCaaa                                                                                                                                              <  2:442901/150‑1 (MQ=255)

          cGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAAc                                                                                                                                             >  2:647652/1‑150 (MQ=255)

            cACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGa                                                                                                                                           <  2:1512323/150‑1 (MQ=255)

              ccGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAg                                                                                                                                         <  1:2877998/150‑1 (MQ=255)

                ggCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAgtg                                                                                                                                       <  2:1941376/150‑1 (MQ=255)

                ggCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAgtg                                                                                                                                       >  2:4070837/1‑150 (MQ=255)

                ggCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACAACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAgtg                                                                                                                                       >  1:3017378/1‑150 (MQ=255)

                     cGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTtgtg                                                                                                                                  >  1:1204797/1‑150 (MQ=255)

                       tAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGc                                                                                                                                >  2:4475645/1‑150 (MQ=255)

                       tAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGc                                                                                                                                >  2:2241359/1‑150 (MQ=255)

                            ggCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACCTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTg                                                                                                                           <  2:4228377/150‑1 (MQ=255)

                            ggCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACCTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTg                                                                                                                           <  2:4228408/150‑1 (MQ=255)

                               aaaCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTAt                                                                                                                        >  1:70781/1‑150 (MQ=255)

                                       tGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACgaaga                                                                                                                >  1:3563944/1‑150 (MQ=255)

                                          aGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAagaaga                                                                                                             >  2:93123/1‑150 (MQ=255)

                                             cGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGt                                                                                                          <  1:2720349/150‑1 (MQ=255)

                                              ggTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTg                                                                                                         >  1:4413694/1‑150 (MQ=255)

                                              ggTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTg                                                                                                         <  2:1357185/150‑1 (MQ=255)

                                                  tgttgtTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACgg                                                                                                     <  2:122534/150‑1 (MQ=255)

                                                  tgttgtTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACgg                                                                                                     >  2:183750/1‑150 (MQ=255)

                                                   gttgttGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGt                                                                                                    >  2:2140616/1‑150 (MQ=255)

                                                    ttgttgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGtt                                                                                                   <  1:3756093/150‑1 (MQ=255)

                                                    ttgttgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGtt                                                                                                   <  1:550147/150‑1 (MQ=255)

                                                    ttgttgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGtt                                                                                                   <  1:550400/150‑1 (MQ=255)

                                                     tgttgCAGGTATTGCCTGCGCAAAATGCCCAGCTGGACGACTTTGACCTCCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGttt                                                                                                  <  1:444125/150‑1 (MQ=255)

                                                      gttgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTa                                                                                                 >  2:2434930/1‑150 (MQ=255)

                                                        tgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACg                                                                                               <  2:2247776/150‑1 (MQ=255)

                                                        tgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATAAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACg                                                                                               >  2:3944566/1‑150 (MQ=255)

                                                        tgCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATAAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGGGTTGTTGCGATTGTATCACGAAGAAGAGGTGACGGTTTACg                                                                                               >  2:3944605/1‑150 (MQ=255)

                                                          cAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGAt                                                                                             <  2:3657916/150‑1 (MQ=255)

                                                           aGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATc                                                                                            <  2:2790503/150‑1 (MQ=255)

                                                            ggTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATcc                                                                                           <  2:3182031/150‑1 (MQ=255)

                                                             gTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCg                                                                                          <  2:3547152/150‑1 (MQ=255)

                                                              tAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGc                                                                                         >  1:3780593/1‑150 (MQ=255)

                                                               aaTGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCa                                                                                        <  1:2130052/150‑1 (MQ=255)

                                                                aTGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAg                                                                                       <  1:3920856/150‑1 (MQ=255)

                                                                aTGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAg                                                                                       <  1:3919395/150‑1 (MQ=255)

                                                                       cgcAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTgg                                                                                <  2:3788268/150‑1 (MQ=255)

                                                                          aaaaTGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGt                                                                             <  1:2900684/150‑1 (MQ=255)

                                                                            aaTGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTc                                                                           <  2:1738511/150‑1 (MQ=255)

                                                                             aTGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCa                                                                          <  2:1644111/150‑1 (MQ=255)

                                                                              tGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCaa                                                                         >  2:833782/1‑150 (MQ=255)

                                                                                cccAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAAt                                                                       >  1:2821812/1‑150 (MQ=255)

                                                                                 ccAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTAAAATg                                                                      >  1:657361/1‑150 (MQ=255)

                                                                                   agcagGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCa                                                                    >  1:555771/1‑150 (MQ=255)

                                                                                   agcagGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCa                                                                    >  2:2668796/1‑150 (MQ=255)

                                                                                      agGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCt                                                                 <  2:3360018/150‑1 (MQ=255)

                                                                                      agGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCt                                                                 >  2:211321/1‑150 (MQ=255)

                                                                                       ggACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTg                                                                <  2:1630253/150‑1 (MQ=255)

                                                                                          cgacTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTc                                                             >  1:1424899/1‑150 (MQ=255)

                                                                                          cgacTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTc                                                             >  1:1074512/1‑150 (MQ=255)

                                                                                                tGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGa                                                       <  2:3417719/150‑1 (MQ=255)

                                                                                                tGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGa                                                       >  2:1292458/1‑150 (MQ=255)

                                                                                                 gACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGaa                                                      >  2:827960/1‑150 (MQ=255)

                                                                                                  accaccTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAAc                                                     <  1:3360110/150‑1 (MQ=255)

                                                                                                   ccaccTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACg                                                    <  1:427098/150‑1 (MQ=255)

                                                                                                     accTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGtt                                                  >  1:991856/1‑150 (MQ=255)

                                                                                                       cTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTgc                                                <  2:2035981/150‑1 (MQ=255)

                                                                                                          gCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGcc                                             <  1:1422463/150‑1 (MQ=255)

                                                                                                            gACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGa                                           <  1:4323435/150‑1 (MQ=255)

                                                                                                             aCGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGAt                                          <  2:381218/150‑1 (MQ=255)

                                                                                                              cGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATg                                         >  2:1850920/1‑150 (MQ=255)

                                                                                                               gCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATgc                                        >  1:369776/1‑150 (MQ=255)

                                                                                                               gCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATgc                                        <  1:524713/150‑1 (MQ=255)

                                                                                                               gCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATgc                                        <  1:3091125/150‑1 (MQ=255)

                                                                                                                cTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATgcg                                       <  2:2903275/150‑1 (MQ=255)

                                                                                                                 tAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATgcgc                                      >  2:2561650/1‑150 (MQ=255)

                                                                                                                     cGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTAAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGaa                                  <  1:3885209/150‑1 (MQ=255)

                                                                                                                     cGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTAAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGaa                                  <  1:762820/150‑1 (MQ=255)

                                                                                                                       aaaCCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGaaaa                                <  2:2671681/150‑1 (MQ=255)

                                                                                                                        aaCCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAAc                               <  1:4100105/150‑1 (MQ=255)

                                                                                                                        aaCCATCAAAACCGAAGAACTGCTGACCTAACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAAc                               <  1:183750/150‑1 (MQ=255)

                                                                                                                         aCCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACg                              >  1:943887/1‑150 (MQ=255)

                                                                                                                          ccATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGc                             <  2:2642008/150‑1 (MQ=255)

                                                                                                                          ccATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGc                             <  2:344804/150‑1 (MQ=255)

                                                                                                                            aTCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTg                           >  2:571250/1‑150 (MQ=255)

                                                                                                                            aTCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTg                           >  2:223334/1‑150 (MQ=255)

                                                                                                                              cAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGcc                         >  1:2004116/1‑150 (MQ=255)

                                                                                                                              cAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGcc                         >  1:3168454/1‑150 (MQ=255)

                                                                                                                                 aaCCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCtga                      >  1:230018/1‑150 (MQ=255)

                                                                                                                                  aCCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCtgat                     <  2:1269214/150‑1 (MQ=255)

                                                                                                                                    cGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCtgatga                   <  2:1740648/150‑1 (MQ=255)

                                                                                                                                     gaagaaCTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATgaa                  >  2:3371126/1‑150 (MQ=255)

                                                                                                                                       agaaCTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATgaaga                >  2:3133241/1‑150 (MQ=255)

                                                                                                                                          aCTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGt             <  1:450461/150‑1 (MQ=255)

                                                                                                                                            tgctgACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTg           >  1:2759826/1‑150 (MQ=255)

                                                                                                                                            tgctgACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTg           >  2:2940895/1‑150 (MQ=255)

                                                                                                                                             gctgACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGa          <  1:3886093/150‑1 (MQ=255)

                                                                                                                                              ctgACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGAt         <  2:1920200/150‑1 (MQ=255)

                                                                                                                                                 aCCATACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGATAGc      >  2:236381/1‑150 (MQ=255)

                                                                                                                                                   cTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGATAGCAt    <  1:779036/150‑1 (MQ=255)

                                                                                                                                                     tACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGATAGCATcc  <  2:4114849/150‑1 (MQ=255)

CCTGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGTGGCGTTTGTATCACGAAGAAGAGGTGACGGTTTACGATCCGCAGGATGTGGAGTTCAAATGCACCTGCTCGCGTGAACGTTGCGCCGATGCGCTGAAAACGCTGCCTGATGAAGAAGTTGATAGCATCC  >  CP009273/3523591‑3523889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`