Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,557,815 T→A 44.8% K709N (AAA→AAT)  glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,557,8150TA44.8% 88.8 / inf 248K709N (AAA→AAT) glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (67/70);  new base A (41/70);  total (108/140)
Fisher's exact test for biased strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 255 total aligned reads displayed.

TCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTT  >  CP009273/3557666‑3557964
                                                                                                                                                     |                                                                                                                                                     
  cGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGttt                                                                                                                                                     >  1:175988/1‑150 (MQ=255)
      aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAg                                                                                                                                                 <  1:238484/150‑1 (MQ=255)
      aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAg                                                                                                                                                 <  1:1676812/150‑1 (MQ=255)
      aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAg                                                                                                                                                 >  2:1864169/1‑150 (MQ=255)
      aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAg                                                                                                                                                 <  2:3585026/150‑1 (MQ=255)
      aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAg                                                                                                                                                 <  1:2069192/150‑1 (MQ=255)
       gTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGc                                                                                                                                                >  2:628548/1‑150 (MQ=255)
        tACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGcc                                                                                                                                               >  2:2007969/1‑150 (MQ=255)
         aCCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGccc                                                                                                                                              >  1:745521/1‑150 (MQ=255)
         aCCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGccc                                                                                                                                              >  1:2375860/1‑150 (MQ=255)
          ccTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCt                                                                                                                                             <  2:1002032/150‑1 (MQ=255)
            tGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga                                                                                                                                           >  1:3090043/1‑150 (MQ=255)
              gTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacg                                                                                                                                         >  2:3455635/1‑150 (MQ=255)
              gTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTgacg                                                                                                                                         >  2:3134429/1‑150 (MQ=255)
              gTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTgacg                                                                                                                                         <  1:4315402/150‑1 (MQ=255)
                aGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacgac                                                                                                                                       <  1:2343751/150‑1 (MQ=255)
                  tGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGc                                                                                                                                     <  2:3290293/150‑1 (MQ=255)
                        ccGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGttct                                                                                                                               <  2:1045460/150‑1 (MQ=255)
                         cGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGttctt                                                                                                                              >  2:3139764/1‑150 (MQ=255)
                         cGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGttctt                                                                                                                              >  2:2730580/1‑150 (MQ=255)
                            aGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCa                                                                                                                           <  2:3176103/150‑1 (MQ=255)
                            aGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCa                                                                                                                           >  1:2049576/1‑150 (MQ=255)
                             gTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCAc                                                                                                                          <  2:3577541/150‑1 (MQ=255)
                             gTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCAc                                                                                                                          <  2:1439893/150‑1 (MQ=255)
                               tGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCActt                                                                                                                        <  2:2250746/150‑1 (MQ=255)
                                gATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCTCGCGGATTGTAGCCCTGACGACGCAGTTCTTCCActtc                                                                                                                       <  2:1367175/150‑1 (MQ=255)
                                gATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtc                                                                                                                       >  1:2162008/1‑150 (MQ=255)
                                 aTCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtct                                                                                                                      <  1:1355636/150‑1 (MQ=255)
                                 aTCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCActtct                                                                                                                      <  1:4082586/150‑1 (MQ=255)
                                  tCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCActtctt                                                                                                                     >  1:4532347/1‑150 (MQ=255)
                                  tCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCATCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGGTCTTCCActtctt                                                                                                                     >  2:1511086/1‑150 (MQ=255)
                                   cAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACttcttc                                                                                                                    >  1:1816555/1‑150 (MQ=255)
                                    aGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTcc                                                                                                                   <  2:2957481/150‑1 (MQ=255)
                                    aGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTcc                                                                                                                   <  1:3505515/150‑1 (MQ=255)
                                     gCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCg                                                                                                                  <  2:1238968/150‑1 (MQ=255)
                                      cGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGc                                                                                                                 >  1:764355/1‑150 (MQ=255)
                                      cGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGc                                                                                                                 >  1:2712262/1‑150 (MQ=255)
                                           cAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTgtgt                                                                                                            >  1:2584676/1‑150 (MQ=255)
                                            aaCCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGtt                                                                                                           <  2:175988/150‑1 (MQ=255)
                                             aCCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTa                                                                                                          <  2:2812711/150‑1 (MQ=255)
                                              ccAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTAc                                                                                                         >  2:850748/1‑150 (MQ=255)
                                               cAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTAcc                                                                                                        <  1:4531858/150‑1 (MQ=255)
                                               cAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTAcc                                                                                                        <  1:622328/150‑1 (MQ=255)
                                                  aTCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCaaa                                                                                                     >  1:2057228/1‑150 (MQ=255)
                                                  aTCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCaaa                                                                                                     <  2:3996901/150‑1 (MQ=255)
                                                     gcgATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGACGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAAt                                                                                                  <  1:366036/150‑1 (MQ=255)
                                                      cgATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATa                                                                                                 <  1:4249170/150‑1 (MQ=255)
                                                       gATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATaa                                                                                                >  2:3769966/1‑150 (MQ=255)
                                                       gATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATaa                                                                                                >  2:1197488/1‑150 (MQ=255)
                                                          aGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGa                                                                                             <  2:3488698/150‑1 (MQ=255)
                                                           gCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGat                                                                                            >  1:2654281/1‑150 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                          >  1:3701380/1‑150 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                          <  2:4116042/150‑1 (MQ=255)
                                                             gACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGatat                                                                                          <  2:4376778/150‑1 (MQ=255)
                                                              aCCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATAtt                                                                                         <  2:110712/150‑1 (MQ=255)
                                                                 cGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTc                                                                                      <  1:4482756/150‑1 (MQ=255)
                                                                  ggATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCa                                                                                     <  1:2954461/150‑1 (MQ=255)
                                                                  ggATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCa                                                                                     <  1:1812355/150‑1 (MQ=255)
                                                                        tCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCg                                                                               <  2:2193921/150‑1 (MQ=255)
                                                                          cGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGAc                                                                             <  2:4147751/150‑1 (MQ=255)
                                                                             aCTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATg                                                                          >  2:3916289/1‑150 (MQ=255)
                                                                             aCTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGAGTTACCAAAAATAAAGATATTGTCAGCACCGACATg                                                                          >  2:8431/1‑150 (MQ=255)
                                                                             aCTGAAAACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATg                                                                          <  2:3779410/150‑1 (MQ=255)
                                                                                  aTACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCa                                                                     >  1:3762635/1‑150 (MQ=255)
                                                                                      acCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAt                                                                 <  2:1688308/150‑1 (MQ=255)
                                                                                         gCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAtctc                                                              <  1:126708/150‑1 (MQ=255)
                                                                                         gCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAtctc                                                              <  2:3952071/150‑1 (MQ=255)
                                                                                          cTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCAGACGGCGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCg                                                             <  1:2936907/150‑1 (MQ=255)
                                                                                             ccGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACa                                                          <  1:1327024/150‑1 (MQ=255)
                                                                                              cGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACAt                                                         <  1:3134456/150‑1 (MQ=255)
                                                                                              cGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACAt                                                         <  1:3134429/150‑1 (MQ=255)
                                                                                                   tGCGTCAGCACCTGATGCAGCTCCTCCTCTTTCTCGTAGTAGTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGATGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCa                                                    <  1:3844864/150‑1 (MQ=255)
                                                                                                   tGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCa                                                    <  1:1470313/150‑1 (MQ=255)
                                                                                                    gCGTCAGCACCTGATGCTGCTCCTCCTCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCTGCTCCGACATGATCCAGCATCTCGACATTCGCAc                                                   <  1:2775415/150‑1 (MQ=255)
                                                                                                     cGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCAcc                                                  <  2:4332168/150‑1 (MQ=255)
                                                                                                             gcTGATGCAGCTCATCATCTTTCTCGTAGTAGTCACGCGGTTTGTAGCCCTGACGACGCAGTTATTCCACTTCTTCCGCTGTGTTACCATAAATAATGATATTGTCAGCACCGACATGATCCAGAATCTCGACATTCGCACCGTCCAACg                                          <  2:2076550/149‑1 (MQ=255)
                                                                                                              cTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGt                                         >  2:401940/1‑150 (MQ=255)
                                                                                                                gATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTAc                                       <  1:491187/150‑1 (MQ=255)
                                                                                                                 aTGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTAcc                                      <  1:1476670/150‑1 (MQ=255)
                                                                                                                   gCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGa                                    >  2:4503190/1‑150 (MQ=255)
                                                                                                                       ctcctcATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGt                                >  1:2645595/1‑150 (MQ=255)
                                                                                                                       ctcctcATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGt                                <  1:393458/150‑1 (MQ=255)
                                                                                                                              tCTTTCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCa                         <  1:2121341/150‑1 (MQ=255)
                                                                                                                                tttCTCGTAGTATTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCAcc                       <  1:360539/150‑1 (MQ=255)
                                                                                                                                  tctcGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGt                     <  1:1056904/150‑1 (MQ=255)
                                                                                                                                      gtagtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAg                 >  2:1362065/1‑150 (MQ=255)
                                                                                                                                          taTTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCa             >  2:1612999/1‑150 (MQ=255)
                                                                                                                                           aTTCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCaa            >  1:1136651/1‑150 (MQ=255)
                                                                                                                                            ttCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCaaa           <  1:2725586/150‑1 (MQ=255)
                                                                                                                                            ttCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCaaa           <  1:436927/150‑1 (MQ=255)
                                                                                                                                            ttCACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCaaa           <  1:1493201/150‑1 (MQ=255)
                                                                                                                                             tCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAAc          >  1:1013579/1‑150 (MQ=255)
                                                                                                                                             tCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAAc          <  1:4277340/150‑1 (MQ=255)
                                                                                                                                             tCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAAc          >  1:1013353/1‑150 (MQ=255)
                                                                                                                                              cACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTACGCGCAAACt         >  1:1204226/1‑150 (MQ=255)
                                                                                                                                              cACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACt         <  2:2049576/150‑1 (MQ=255)
                                                                                                                                              cACGCGGATTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACt         <  1:3065606/150‑1 (MQ=255)
                                                                                                                                                cgcgGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTc       <  1:3416906/150‑1 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCagg    >  1:2812353/1‑148 (MQ=255)
                                                                                                                                                   ggTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATg    >  1:2812484/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTT  >  CP009273/3557666‑3557964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑