Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,561,574 C→A 100% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,561,5740CA96.5% 739.1 / 15.1 229W372L (TGG→TTG) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base A (128/93);  total (131/98)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

ATTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAC  >  CP009273/3561427‑3561718
                                                                                                                                                   |                                                                                                                                                
aTTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCAt                                                                                                                                                <  2:2245039/150‑1 (MQ=255)
aTTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAt                                                                                                                                                >  2:4461837/1‑150 (MQ=255)
aTTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAt                                                                                                                                                >  2:2395535/1‑150 (MQ=255)
 tttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATa                                                                                                                                               >  2:2116693/1‑150 (MQ=255)
  ttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATAc                                                                                                                                              >  2:3221937/1‑150 (MQ=255)
    cgcgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACtt                                                                                                                                            >  1:2124363/1‑150 (MQ=255)
       gcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCg                                                                                                                                         <  1:845055/150‑1 (MQ=255)
        cgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCgg                                                                                                                                        >  2:2482224/1‑150 (MQ=255)
         gTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGt                                                                                                                                       >  1:3115983/1‑150 (MQ=255)
          ttACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                      <  1:2335003/150‑1 (MQ=255)
           tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTaa                                                                                                                                     >  1:2164114/1‑150 (MQ=255)
            aCCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAAc                                                                                                                                    <  2:877050/150‑1 (MQ=255)
             ccAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACa                                                                                                                                   <  2:2778078/150‑1 (MQ=255)
               aGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAAt                                                                                                                                 <  2:4270007/150‑1 (MQ=255)
                gCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATa                                                                                                                                <  2:3055163/150‑1 (MQ=255)
                 cACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATAc                                                                                                                               >  1:2471950/1‑150 (MQ=255)
                  aCGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcg                                                                                                                              <  1:1686273/150‑1 (MQ=255)
                   cGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATAcgg                                                                                                                             <  1:397844/150‑1 (MQ=255)
                   cGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                             >  1:3716185/1‑150 (MQ=255)
                       gCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGa                                                                                                                         <  1:1652792/150‑1 (MQ=255)
                         cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAAt                                                                                                                       >  1:3066255/1‑150 (MQ=255)
                           tcttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCa                                                                                                                     <  1:2181260/150‑1 (MQ=255)
                            cttctGAACTATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCaa                                                                                                                    >  1:3840569/1‑150 (MQ=255)
                             ttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAAt                                                                                                                   >  1:1624044/1‑150 (MQ=255)
                             ttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAAt                                                                                                                   <  2:1064210/150‑1 (MQ=255)
                                  aaCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                              >  1:417893/1‑150 (MQ=255)
                                   aCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCa                                                                                                             <  2:2404803/150‑1 (MQ=255)
                                   aCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCa                                                                                                             <  2:1798430/150‑1 (MQ=255)
                                    cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGACTCAATGTTGCAg                                                                                                            >  2:98296/1‑150 (MQ=255)
                                    cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAg                                                                                                            >  2:100445/1‑150 (MQ=255)
                                       tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                         <  1:3081293/150‑1 (MQ=255)
                                        aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatg                                                                                                        >  1:1325753/1‑150 (MQ=255)
                                        aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatg                                                                                                        >  2:3995586/1‑150 (MQ=255)
                                          aaaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                      >  1:2666652/1‑150 (MQ=255)
                                          aaaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                      >  1:2314178/1‑150 (MQ=255)
                                            acgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAAtt                                                                                                    <  2:2282196/150‑1 (MQ=255)
                                             cgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTc                                                                                                   >  1:2815458/1‑150 (MQ=255)
                                              gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatg                                                                                                        >  1:4385123/1‑144 (MQ=255)
                                              gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatg                                                                                                        <  2:4385123/144‑1 (MQ=255)
                                              gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                  >  2:695082/1‑150 (MQ=255)
                                              gacgTGCAACTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAAACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAAATCa                                                                                                  >  1:338485/1‑150 (MQ=255)
                                                cgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcg                                                                                                >  2:342890/1‑150 (MQ=255)
                                                cgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcg                                                                                                >  2:3986068/1‑150 (MQ=255)
                                                cgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcg                                                                                                >  1:1982190/1‑150 (MQ=255)
                                                 gTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                               >  1:1218476/1‑150 (MQ=255)
                                                      tCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcg                                                                                          <  2:1064062/150‑1 (MQ=255)
                                                       cTTCCTCTGCGTTTTCACCACTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgc                                                                                         <  2:4224633/150‑1 (MQ=255)
                                                        ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcg                                                                                        <  2:3776472/150‑1 (MQ=255)
                                                        ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcg                                                                                        <  2:954542/150‑1 (MQ=255)
                                                        ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcg                                                                                        <  2:954074/150‑1 (MQ=255)
                                                             ctGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgaga                                                                                   >  2:3355936/1‑150 (MQ=255)
                                                                   tttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaa                                                                             <  2:3799687/150‑1 (MQ=255)
                                                                     tCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACg                                                                           <  1:985389/150‑1 (MQ=255)
                                                                     tCACCAAGCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACg                                                                           <  2:480357/150‑1 (MQ=255)
                                                                      cACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACgg                                                                          <  2:3064091/150‑1 (MQ=255)
                                                                         cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACt                                                                       <  1:2762701/150‑1 (MQ=255)
                                                                             cACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcac                                                                   >  2:211358/1‑150 (MQ=255)
                                                                              aCCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcacc                                                                  >  2:3663199/1‑150 (MQ=255)
                                                                               ccATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcacca                                                                 >  2:1418576/1‑150 (MQ=255)
                                                                                  tGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacca                                                              >  2:1953714/1‑150 (MQ=255)
                                                                                  agCCTACCGGAATAACACAATCACGAACGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACAACCGGTAACAATACGGAGGAATCAAAGTAGCAGAATGAATTCACGAGACCGCTCGAGAACAGAACGCACTGaaccacaa                                                              >  2:1953670/2‑148 (MQ=255)
                                                                                    ccTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCaccaccacc                                                            <  2:2682416/150‑1 (MQ=255)
                                                                                    ccTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCaccaccacc                                                            <  2:2682401/150‑1 (MQ=255)
                                                                                    ccTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCaccaccacc                                                            <  2:387826/150‑1 (MQ=255)
                                                                                     cTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCg                                                           >  1:3054736/1‑150 (MQ=255)
                                                                                         cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                       >  1:2020027/1‑150 (MQ=255)
                                                                                         cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                       >  1:2020020/1‑150 (MQ=255)
                                                                                              tAACACAAGCACGATCGATTACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGgaga                                                  >  2:3310281/1‑150 (MQ=255)
                                                                                               aaCACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaat                                                                                                     >  2:663369/1‑98 (MQ=255)
                                                                                               aaCACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGAt                                                 <  1:552551/150‑1 (MQ=255)
                                                                                               aaCACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGAt                                                 >  1:2857343/1‑150 (MQ=255)
                                                                                                    aaGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                            >  1:784890/1‑150 (MQ=255)
                                                                                                    aaGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                            >  2:1622129/1‑150 (MQ=255)
                                                                                                    aaGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                            >  2:1262599/1‑150 (MQ=255)
                                                                                                     aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacaca                                           <  1:1615642/150‑1 (MQ=255)
                                                                                                     aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacaca                                           <  1:3090384/150‑1 (MQ=255)
                                                                                                     aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacaca                                           <  2:2932513/150‑1 (MQ=255)
                                                                                                      gCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACaa                                          >  2:206120/1‑150 (MQ=255)
                                                                                                      gCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACaa                                          >  2:2558730/1‑150 (MQ=255)
                                                                                                         cgatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccg                                       <  1:4422728/150‑1 (MQ=255)
                                                                                                              gatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGa                                  >  1:1431920/1‑150 (MQ=255)
                                                                                                               atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                 >  1:1266509/1‑150 (MQ=255)
                                                                                                               atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                 >  1:398343/1‑150 (MQ=255)
                                                                                                               atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                 >  1:658462/1‑150 (MQ=255)
                                                                                                               atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                 >  1:397001/1‑150 (MQ=255)
                                                                                                                tGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaaa                                >  1:2836703/1‑150 (MQ=255)
                                                                                                                   cGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                             >  1:1860706/1‑150 (MQ=255)
                                                                                                                   cGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                             <  1:1875126/150‑1 (MQ=255)
                                                                                                                   cGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                             <  2:2164304/150‑1 (MQ=255)
                                                                                                                         ggcgCGGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGTGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGt                       >  1:976441/1‑150 (MQ=255)
                                                                                                                         ggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGt                       <  1:2843498/150‑1 (MQ=255)
                                                                                                                            gcAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTaa                    >  1:1384545/1‑150 (MQ=255)
                                                                                                                             cAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAg                   <  2:1748435/150‑1 (MQ=255)
                                                                                                                                   gCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCAt             <  1:1555256/150‑1 (MQ=255)
                                                                                                                                     aCGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATcc           >  1:4073424/1‑150 (MQ=255)
                                                                                                                                      cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc          <  1:3236930/150‑1 (MQ=255)
                                                                                                                                       gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCg         <  1:101703/150‑1 (MQ=255)
                                                                                                                                       gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCg         <  1:3007835/150‑1 (MQ=255)
                                                                                                                                         gCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTg       <  1:3394123/150‑1 (MQ=255)
                                                                                                                                           gACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc     >  1:3530548/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAC  >  CP009273/3561427‑3561718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑