Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,568,708 C→A 5.8% S93* (TCA→TAA)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,568,7080CA 5.8% 1073.0 / 23.3 284S93* (TCA→TAA) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (139/128);  new base A (10/7);  total (149/135)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

TTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCT  >  CP009273/3568560‑3568855
                                                                                                                                                    |                                                                                                                                                   
ttGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCa                                                                                                                                                    <  1:2389098/150‑1 (MQ=255)
ttGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCa                                                                                                                                                    <  1:227814/150‑1 (MQ=255)
   cTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGa                                                                                                                                                 <  2:910842/150‑1 (MQ=255)
     tATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGaaa                                                                                                                                               >  1:1703979/1‑150 (MQ=255)
        tGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATag                                                                                                                                            >  2:371298/1‑150 (MQ=255)
         gCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagc                                                                                                                                           <  1:270444/150‑1 (MQ=255)
            ctcCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagcagc                                                                                                                                        <  2:1750911/150‑1 (MQ=255)
             tcCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCg                                                                                                                                       <  1:2866539/150‑1 (MQ=255)
              ccTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCgg                                                                                                                                      <  2:2121365/150‑1 (MQ=255)
                  gTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCtt                                                                                                                                  >  2:3819324/1‑150 (MQ=255)
                    gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                <  1:105632/150‑1 (MQ=255)
                    gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                <  1:4128905/150‑1 (MQ=255)
                        cTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCa                                                                                                                            <  2:1735605/150‑1 (MQ=255)
                         tGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAg                                                                                                                           >  2:4107529/1‑150 (MQ=255)
                         tGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAg                                                                                                                           >  2:4107915/1‑150 (MQ=255)
                          ggTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAgg                                                                                                                          <  2:1041208/150‑1 (MQ=255)
                              ttCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGac                                                                                                                      >  2:2926817/1‑149 (MQ=255)
                               tCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAag                                                                                                                     <  1:1179995/150‑1 (MQ=255)
                                  tGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGc                                                                                                                  >  1:2100094/1‑150 (MQ=255)
                                   gTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGcc                                                                                                                 >  1:4498822/1‑150 (MQ=255)
                                    tttGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTTAAGAGCCa                                                                                                                >  1:4457758/1‑150 (MQ=255)
                                    tttGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCa                                                                                                                <  1:4301272/150‑1 (MQ=255)
                                      tGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCAtt                                                                                                              >  2:2040586/1‑150 (MQ=255)
                                        cGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTa                                                                                                            <  1:1904350/150‑1 (MQ=255)
                                         gggTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTAt                                                                                                           <  2:4373738/150‑1 (MQ=255)
                                         gggTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTAt                                                                                                           <  1:3781995/150‑1 (MQ=255)
                                          ggTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATc                                                                                                          <  1:804856/150‑1 (MQ=255)
                                             gagaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTg                                                                                                       <  2:4508004/150‑1 (MQ=255)
                                             gagaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTg                                                                                                       >  1:1094379/1‑150 (MQ=255)
                                              agaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGc                                                                                                      >  2:2327252/1‑150 (MQ=255)
                                               gaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGcc                                                                                                     <  1:1003916/150‑1 (MQ=255)
                                               gaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGcc                                                                                                     >  1:1563764/1‑150 (MQ=255)
                                                aaaaTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCg                                                                                                    <  2:3861102/150‑1 (MQ=255)
                                                  aaTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGtt                                                                                                  <  2:352690/150‑1 (MQ=255)
                                                     tCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGc                                                                                               >  2:1808390/1‑150 (MQ=255)
                                                        ggCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAAt                                                                                            >  1:4232491/1‑150 (MQ=255)
                                                         gCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAAtt                                                                                           <  2:2945147/150‑1 (MQ=255)
                                                         gCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAAtt                                                                                           <  2:156464/150‑1 (MQ=255)
                                                          cATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGAATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGTTGAAGAGCCATTTATCGTGCCGTTGGCAATTc                                                                                          >  2:514214/1‑150 (MQ=255)
                                                            tttCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCg                                                                                        <  1:2778798/150‑1 (MQ=255)
                                                              tCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGtt                                                                                      <  2:4184586/150‑1 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                  >  1:2052883/1‑150 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                  <  1:3003214/150‑1 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                  <  2:656912/150‑1 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATGTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                  <  2:3582297/150‑1 (MQ=255)
                                                                     cTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGcc                                                                               <  1:2449929/150‑1 (MQ=255)
                                                                        cGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCggg                                                                            <  2:803480/150‑1 (MQ=255)
                                                                          aGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGcc                                                                          >  2:2360380/1‑150 (MQ=255)
                                                                          aGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGcc                                                                          >  1:489548/1‑150 (MQ=255)
                                                                          aGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATGTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGcc                                                                          >  2:1437907/1‑150 (MQ=255)
                                                                           gCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCg                                                                         <  1:1908560/150‑1 (MQ=255)
                                                                           gCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCg                                                                         <  2:339833/150‑1 (MQ=255)
                                                                            cAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGa                                                                        >  2:4292739/1‑150 (MQ=255)
                                                                            cAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCAGTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGa                                                                        >  2:4295484/1‑150 (MQ=255)
                                                                             aGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGAc                                                                       >  2:2786890/1‑150 (MQ=255)
                                                                               aaaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTa                                                                     <  2:2385268/150‑1 (MQ=255)
                                                                                 aCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAtt                                                                   <  2:2882864/150‑1 (MQ=255)
                                                                                    gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                <  2:1703979/150‑1 (MQ=255)
                                                                                    gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                >  1:524520/1‑150 (MQ=255)
                                                                                    gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                >  1:564567/1‑150 (MQ=255)
                                                                                     tctcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCg                                                               >  1:4094649/1‑150 (MQ=255)
                                                                                        ccATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCa                                                            <  2:615909/150‑1 (MQ=255)
                                                                                          aTTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACg                                                          >  1:2558456/1‑150 (MQ=255)
                                                                                              cTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGa                                                      >  2:1061608/1‑150 (MQ=255)
                                                                                              cTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGa                                                      >  2:3342083/1‑150 (MQ=255)
                                                                                               tGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGATTTAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAt                                                     <  1:1363921/150‑1 (MQ=255)
                                                                                               tGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAt                                                     <  1:1538256/150‑1 (MQ=255)
                                                                                               tGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAt                                                     <  1:3633399/150‑1 (MQ=255)
                                                                                               tGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAt                                                     >  2:2612347/1‑150 (MQ=255)
                                                                                                ggcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtg                                                    <  2:1657667/150‑1 (MQ=255)
                                                                                                 gcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgt                                                   <  1:373464/150‑1 (MQ=255)
                                                                                                    gcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgt                                                >  1:1809203/1‑150 (MQ=255)
                                                                                                     catcatTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgtt                                               >  1:2998446/1‑150 (MQ=255)
                                                                                                      atcatTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgttg                                              >  2:1336990/1‑150 (MQ=255)
                                                                                                       tcatTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgttgt                                             >  2:2218679/1‑150 (MQ=255)
                                                                                                       tcatTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgttgt                                             >  2:2218737/1‑150 (MQ=255)
                                                                                                        catTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGtc                                            >  2:2576757/1‑150 (MQ=255)
                                                                                                         atTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGtct                                           <  1:685846/150‑1 (MQ=255)
                                                                                                              gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCCCGCTGATGTTGTTGTCtcatca                                      >  1:2711562/1‑150 (MQ=255)
                                                                                                              gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                      >  2:1553137/1‑150 (MQ=255)
                                                                                                               tttCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAg                                     >  2:161110/1‑150 (MQ=255)
                                                                                                                  cTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAc                                  <  2:3387903/150‑1 (MQ=255)
                                                                                                                  cTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAc                                  <  2:1266265/150‑1 (MQ=255)
                                                                                                                   tGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAcc                                 >  2:1518397/1‑150 (MQ=255)
                                                                                                                   tGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAcc                                 >  1:2583029/1‑150 (MQ=255)
                                                                                                                       cGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGaa                             >  2:3117347/1‑150 (MQ=255)
                                                                                                                         ccATTAAAATGATGTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATc                           <  2:2537890/150‑1 (MQ=255)
                                                                                                                          cATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCa                          <  2:4529675/150‑1 (MQ=255)
                                                                                                                           aTTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAg                         <  2:2247437/150‑1 (MQ=255)
                                                                                                                           aTTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAg                         >  2:1152573/1‑150 (MQ=255)
                                                                                                                              aaaaTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                      <  1:917776/150‑1 (MQ=255)
                                                                                                                              aaaaTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                      >  1:1447498/1‑150 (MQ=255)
                                                                                                                                  tGATTTTGCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGc                  >  2:1121222/1‑150 (MQ=255)
                                                                                                                                        tCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTgg            <  1:1465470/150‑1 (MQ=255)
                                                                                                                                          cccAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTg          <  1:3342083/150‑1 (MQ=255)
                                                                                                                                           ccAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGa         >  2:4023874/1‑150 (MQ=255)
                                                                                                                                           ccAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGa         >  2:2512804/1‑150 (MQ=255)
                                                                                                                                               cgcTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGc     >  1:4059937/1‑150 (MQ=255)
                                                                                                                                               cgcTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGc     <  2:2821066/150‑1 (MQ=255)
                                                                                                                                                  ttCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGctct  >  1:3802478/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCT  >  CP009273/3568560‑3568855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑