Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,574,500 G→T 9.4% M1M (ATG→ATT) † yhhY → putative acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,574,5000GT 9.4% 1023.8 / 50.9 283M1M (ATG→ATT) †yhhYputative acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (142/114);  new base T (18/8);  total (161/122)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 295 total aligned reads displayed.

TCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGC  >  CP009273/3574352‑3574647
                                                                                                                                                    |                                                                                                                                                   
 ctcCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAAtgag                                                                                                                                                   >  1:143544/1‑150 (MQ=255)
         gggTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAg                                                                                                                                           >  1:918099/1‑150 (MQ=255)
         gggTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAg                                                                                                                                           <  2:3471470/150‑1 (MQ=255)
            tCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTaa                                                                                                                                        >  2:3310383/1‑150 (MQ=255)
             cTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAAt                                                                                                                                       <  2:2133902/150‑1 (MQ=255)
                ccTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACg                                                                                                                                    <  2:144139/150‑1 (MQ=255)
                 cTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGTACAAAGGAATGAGTGAGATAGTAATACGc                                                                                                                                   >  2:3337428/1‑150 (MQ=255)
                  tGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGcc                                                                                                                                  >  2:514321/1‑150 (MQ=255)
                   gATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCa                                                                                                                                 >  2:1798136/1‑150 (MQ=255)
                     tCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGAAATACGCCACg                                                                                                                               >  2:2314527/1‑150 (MQ=255)
                             cAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAaca                                                                                                                       >  1:3852590/1‑150 (MQ=255)
                             cAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAaca                                                                                                                       >  2:1573475/1‑150 (MQ=255)
                                taataatCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTACTACGCCACGCAGAAACACgg                                                                                                                    >  2:161671/1‑150 (MQ=255)
                                 aataatCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACggg                                                                                                                   <  2:4242295/150‑1 (MQ=255)
                                    aatCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGAtt                                                                                                                <  2:4493149/150‑1 (MQ=255)
                                      tcattctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTAc                                                                                                              >  2:1071744/1‑150 (MQ=255)
                                      tcattctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTAc                                                                                                              <  2:324646/150‑1 (MQ=255)
                                        attctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTAAATTATGTTGAAATGTAAATTTACCCAAAAGAGGACAAAGTAATGAGTGAGATAGTAATAATCCACGCAGAAAAACGGGATTACGa                                                                                                            >  1:3635237/1‑150 (MQ=255)
                                         ttctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTAACCAAAAGAGGACAAAGGATTGAGTGAGATAGTAATACGCCACGCAGTAACACGGGATTACGAg                                                                                                           <  2:2392064/150‑1 (MQ=255)
                                           ctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGAAGAAACACGGGATTACGAGGc                                                                                                         >  2:2527071/1‑150 (MQ=255)
                                             cattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCa                                                                                                       >  2:4164134/1‑150 (MQ=255)
                                              attcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGACTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCAt                                                                                                      >  2:3818933/1‑150 (MQ=255)
                                              attcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCAt                                                                                                      >  1:2541999/1‑150 (MQ=255)
                                              attcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCAt                                                                                                      >  1:813263/1‑150 (MQ=255)
                                               ttcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATc                                                                                                     <  1:634799/150‑1 (MQ=255)
                                                  gCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAgg                                                                                                  <  2:2045789/150‑1 (MQ=255)
                                                   cACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGc                                                                                                 <  2:1646362/150‑1 (MQ=255)
                                                    aCTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCa                                                                                                >  1:3190388/1‑150 (MQ=255)
                                                    aCTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCCTCAGGCa                                                                                                >  2:142832/1‑150 (MQ=255)
                                                    aCTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCa                                                                                                >  2:1987835/1‑150 (MQ=255)
                                                    aCTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCa                                                                                                >  2:1146512/1‑150 (MQ=255)
                                                     cTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAg                                                                                               >  1:3388317/1‑150 (MQ=255)
                                                     cTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAg                                                                                               <  2:2330937/150‑1 (MQ=255)
                                                        gTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGAtt                                                                                            >  2:3361672/1‑150 (MQ=255)
                                                        gTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGAtt                                                                                            <  1:496381/150‑1 (MQ=255)
                                                           cAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACAGGATTACGAGGCCATCAGGCAGATTCAc                                                                                         >  2:4074211/1‑150 (MQ=255)
                                                                cTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCa                                                                                    <  1:870365/150‑1 (MQ=255)
                                                                cTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCa                                                                                    <  1:2222658/150‑1 (MQ=255)
                                                                cTTTGTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCa                                                                                    <  2:2258726/150‑1 (MQ=255)
                                                                  ttttGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGAGTACGAGGCCATCAGGCAGATTCACGCCCAGc                                                                                  >  2:418789/1‑150 (MQ=255)
                                                                    ttGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCg                                                                                <  1:2405352/150‑1 (MQ=255)
                                                                     tGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCgg                                                                               <  1:2052153/150‑1 (MQ=255)
                                                                        aaaaaaaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAgg                                                                            >  2:3758325/1‑150 (MQ=255)
                                                                        aaaaaaaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAgg                                                                            >  2:2157299/1‑150 (MQ=255)
                                                                        aaaaaaaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAgg                                                                            >  1:2920500/1‑150 (MQ=255)
                                                                          aaaaaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGgtg                                                                          <  1:1167482/150‑1 (MQ=255)
                                                                            aaaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTa                                                                        >  2:2846893/1‑150 (MQ=255)
                                                                             aaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTAt                                                                       >  1:3891781/1‑150 (MQ=255)
                                                                             aaTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTAt                                                                       <  2:3527758/150‑1 (MQ=255)
                                                                              aTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTAtt                                                                      <  1:4076999/150‑1 (MQ=255)
                                                                              aTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTAtt                                                                      >  1:4294250/1‑150 (MQ=255)
                                                                              aTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTAtt                                                                      >  1:3038650/1‑150 (MQ=255)
                                                                                gCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGc                                                                    >  2:982015/1‑150 (MQ=255)
                                                                                 cATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCa                                                                   >  2:3161525/1‑150 (MQ=255)
                                                                                 cATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCa                                                                   >  2:1663341/1‑150 (MQ=255)
                                                                                   tttGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAac                                                                 <  1:4112470/150‑1 (MQ=255)
                                                                                     tGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAacac                                                               >  1:3569439/1‑150 (MQ=255)
                                                                                        cTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACt                                                            >  1:738071/1‑150 (MQ=255)
                                                                                           gCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACa                                                         >  1:2987220/1‑150 (MQ=255)
                                                                                            cATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAg                                                        <  1:1896883/150‑1 (MQ=255)
                                                                                              tttGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGt                                                      <  2:1104640/150‑1 (MQ=255)
                                                                                              tttGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGt                                                      >  2:3570594/1‑150 (MQ=255)
                                                                                                tGAAAGTCAATGATGTTGATAGGGACATTTACCACAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTTCGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGc                                                    <  2:441402/150‑1 (MQ=255)
                                                                                                   aaGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTc                                                 >  2:891485/1‑150 (MQ=255)
                                                                                                   aaGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTc                                                 >  1:4059383/1‑150 (MQ=255)
                                                                                                   aaGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTc                                                 <  2:3015609/150‑1 (MQ=255)
                                                                                                         atgatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCtt                                           <  1:3997405/150‑1 (MQ=255)
                                                                                                         atgatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCtt                                           >  1:2279597/1‑150 (MQ=255)
                                                                                                          tgatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTc                                          >  1:4482287/1‑150 (MQ=255)
                                                                                                          tgatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTc                                          >  1:4314537/1‑150 (MQ=255)
                                                                                                             tgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGa                                       <  1:1530022/150‑1 (MQ=255)
                                                                                                             tgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGa                                       <  1:1365240/150‑1 (MQ=255)
                                                                                                               ttGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATc                                     <  1:1234889/150‑1 (MQ=255)
                                                                                                                 gAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCat                                   <  1:4164134/150‑1 (MQ=255)
                                                                                                                 gAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCat                                   <  1:2036329/150‑1 (MQ=255)
                                                                                                                  aaaGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCata                                  >  1:1758476/1‑150 (MQ=255)
                                                                                                                  aaaGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCata                                  <  2:2936097/150‑1 (MQ=255)
                                                                                                                          aTTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGTTTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTTTTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAg                          <  1:4149025/150‑1 (MQ=255)
                                                                                                                          aTTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAg                          >  2:2638610/1‑150 (MQ=255)
                                                                                                                          aTTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAg                          <  1:3310388/150‑1 (MQ=255)
                                                                                                                           tttACCCCATAGTGGACAAAGGTTTGAGTGAGATAGTAATACGCCACGCTGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGATGTGTATTGCATCACTCTACAGGTGCCTGATCCTTCCGATCATATGTGGCAgg                         <  2:484651/150‑1 (MQ=255)
                                                                                                                           tttACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAgg                         <  2:932270/150‑1 (MQ=255)
                                                                                                                            ttACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGa                        >  1:2522032/1‑150 (MQ=255)
                                                                                                                             tACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAg                       <  1:111860/150‑1 (MQ=255)
                                                                                                                             tACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAg                       <  1:4449522/150‑1 (MQ=255)
                                                                                                                                 ccAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGAc                   >  1:3793260/1‑150 (MQ=255)
                                                                                                                                 ccAAAGAGCACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGAc                   >  1:2505561/1‑150 (MQ=255)
                                                                                                                                  cAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACt                  <  1:1220193/150‑1 (MQ=255)
                                                                                                                                  cAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACt                  <  1:3491221/150‑1 (MQ=255)
                                                                                                                                   aaaGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTc                 <  1:1712088/150‑1 (MQ=255)
                                                                                                                                   aaaGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTc                 >  2:606872/1‑150 (MQ=255)
                                                                                                                                   aaaGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTc                 <  1:2699624/150‑1 (MQ=255)
                                                                                                                                     agagGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGc               >  2:4273101/1‑150 (MQ=255)
                                                                                                                                       agGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCg             >  2:3609967/1‑150 (MQ=255)
                                                                                                                                       agGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCg             >  1:1878149/1‑150 (MQ=255)
                                                                                                                                           cAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCg         >  1:1002774/1‑150 (MQ=255)
                                                                                                                                              aGGAATTAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTcc      <  1:1702545/150‑1 (MQ=255)
                                                                                                                                              aGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTcc      >  1:2331560/1‑150 (MQ=255)
                                                                                                                                              aGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTcc      >  1:2430933/1‑150 (MQ=255)
                                                                                                                                                gAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCg    <  1:514321/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGC  >  CP009273/3574352‑3574647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑