Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,588,904 A→T 11.1% L287Q (CTG→CAG)  livH ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,588,9040AT11.1% 713.9 / 66.4 234L287Q (CTG→CAG) livHleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (98/110);  new base T (14/12);  total (112/122)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 255 total aligned reads displayed.

TCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGC  >  CP009273/3588755‑3589052
                                                                                                                                                     |                                                                                                                                                    
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATTCCGGTCGGCATCACCAGCAGCACCAGTATcagca                                                                                                                                                      <  2:3662738/150‑1 (MQ=255)
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      <  2:44828/150‑1 (MQ=255)
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      <  2:43824/150‑1 (MQ=255)
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      <  2:1578075/150‑1 (MQ=255)
tCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagca                                                                                                                                                      >  1:2363331/1‑150 (MQ=255)
 ccAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagcag                                                                                                                                                     <  2:635083/150‑1 (MQ=255)
    gTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGc                                                                                                                                                  <  1:3962927/150‑1 (MQ=255)
     ttGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCg                                                                                                                                                 <  1:4492720/150‑1 (MQ=255)
      tGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGa                                                                                                                                                <  1:3410229/150‑1 (MQ=255)
        cACGCCCATAAAGACGCCCGCCAGCACAAAGAACCTCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAt                                                                                                                                              <  2:3470577/150‑1 (MQ=255)
        cACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAt                                                                                                                                              <  2:110696/150‑1 (MQ=255)
        cACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAt                                                                                                                                              <  2:1312062/150‑1 (MQ=255)
        cACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAt                                                                                                                                              <  1:2389221/150‑1 (MQ=255)
            cccATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATgaga                                                                                                                                          >  1:4089817/1‑150 (MQ=255)
            cccATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATgaga                                                                                                                                          >  1:1839099/1‑150 (MQ=255)
             ccATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGACTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGAc                                                                                                                                         >  2:3052451/1‑150 (MQ=255)
             ccATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGAc                                                                                                                                         >  2:1861763/1‑150 (MQ=255)
                tAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACcac                                                                                                                                      <  2:2507071/150‑1 (MQ=255)
                 aaaGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACcaca                                                                                                                                     >  2:1422593/1‑150 (MQ=255)
                    gACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCt                                                                                                                                  >  2:3976828/1‑150 (MQ=255)
                     aCGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTTCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCtt                                                                                                                                 <  2:4533808/150‑1 (MQ=255)
                      cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCttt                                                                                                                                <  2:1704578/150‑1 (MQ=255)
                      cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCttt                                                                                                                                <  2:740949/150‑1 (MQ=255)
                        cccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTtat                                                                                                                              >  1:3652359/1‑150 (MQ=255)
                          cgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTatat                                                                                                                            <  2:2210235/150‑1 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTc                                                                                                                          <  1:689486/150‑1 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTc                                                                                                                          <  1:4238761/150‑1 (MQ=255)
                            ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTc                                                                                                                          <  1:1990884/150‑1 (MQ=255)
                             cAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTcc                                                                                                                         >  2:209316/1‑150 (MQ=255)
                             cAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTGTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTcc                                                                                                                         <  2:1171485/150‑1 (MQ=255)
                                   aaaGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTc                                                                                                                   <  2:2695411/150‑1 (MQ=255)
                                      gAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGa                                                                                                                <  1:3308904/150‑1 (MQ=255)
                                       aaCATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGAt                                                                                                               >  2:4104935/1‑150 (MQ=255)
                                       aaCATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGAt                                                                                                               <  1:745590/150‑1 (MQ=255)
                                            cgcgGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCa                                                                                                          <  2:428345/150‑1 (MQ=255)
                                             gcgGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCCCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCag                                                                                                         <  2:2436260/150‑1 (MQ=255)
                                                gCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaag                                                                                                      >  1:1121397/1‑150 (MQ=255)
                                                gCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaag                                                                                                      >  1:3398111/1‑150 (MQ=255)
                                                 cAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaaga                                                                                                     >  2:4028945/1‑150 (MQ=255)
                                                  agagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCATCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagag                                                                                                    >  2:2899676/1‑150 (MQ=255)
                                                  agagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGAAGGGCGAATGAGACCACATCTATATATTCCGTACTCAGAAAGGCAGTagag                                                                                                    >  1:2153852/1‑150 (MQ=255)
                                                    agagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagagag                                                                                                  >  1:1279985/1‑150 (MQ=255)
                                                        cagcGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGcc                                                                                              >  2:385326/1‑150 (MQ=255)
                                                        cagcGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGcc                                                                                              >  1:872471/1‑150 (MQ=255)
                                                        cagcGCCATTGCAATATGCATCGGTTTCATACATTCTCTACCACCGGGCGACACAGAATACCGGTCGGCATCACCATCAGCACCAGAATCAGCAGGGCGAATGATACCACATCTATATATTCCGTACTCAGATAGGCAGAagagagatcc                                                                                              >  1:2666899/1‑146 (MQ=255)
                                                         agcGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCt                                                                                             >  1:1358910/1‑150 (MQ=255)
                                                           cgcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCTGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                           <  1:4434261/150‑1 (MQ=255)
                                                            gcCATTGCAATATGCATCGGATTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGTCATCACCAGCAGCACAAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTACGTACTCAGATAGGCAGAAGAGAGCGCCTCCg                                                                                          >  2:4368217/1‑150 (MQ=255)
                                                               aTTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCaa                                                                                       >  1:1277017/1‑150 (MQ=255)
                                                                ttGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAAt                                                                                      >  2:3794113/1‑150 (MQ=255)
                                                                 tGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATc                                                                                     >  1:1321496/1‑150 (MQ=255)
                                                                   cAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATccc                                                                                   >  1:27526/1‑150 (MQ=255)
                                                                    aaTATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCATGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGAGCCTCCGCAATcccc                                                                                  >  1:1026299/1‑150 (MQ=255)
                                                                      tatGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAg                                                                                <  2:3302520/150‑1 (MQ=255)
                                                                             cGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAgg                                                                         <  1:2184357/150‑1 (MQ=255)
                                                                               gTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgcc                                                                       <  2:4020470/150‑1 (MQ=255)
                                                                                   cATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCa                                                                   >  1:4184004/1‑150 (MQ=255)
                                                                                   cATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCa                                                                   >  2:600330/1‑150 (MQ=255)
                                                                                    aTACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCaa                                                                  >  2:4487307/1‑150 (MQ=255)
                                                                                     tACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAat                                                                 >  2:2073468/1‑150 (MQ=255)
                                                                                     tACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAat                                                                 <  1:3971964/150‑1 (MQ=255)
                                                                                      aCTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatc                                                                >  1:959401/1‑150 (MQ=255)
                                                                                       cTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatca                                                               <  1:767189/150‑1 (MQ=255)
                                                                                       cTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatca                                                               <  1:832892/150‑1 (MQ=255)
                                                                                        tttCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcat                                                              >  1:1422816/1‑150 (MQ=255)
                                                                                         ttCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGACGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcatc                                                             >  1:2779315/1‑150 (MQ=255)
                                                                                          tctcTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCg                                                            >  2:127093/1‑150 (MQ=255)
                                                                                            tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCt                                                          >  1:3070532/1‑150 (MQ=255)
                                                                                            tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCt                                                          >  2:2383851/1‑150 (MQ=255)
                                                                                            tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCt                                                          >  1:71966/1‑150 (MQ=255)
                                                                                             ctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTc                                                         <  2:91254/150‑1 (MQ=255)
                                                                                              tACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTcc                                                        <  2:2830677/150‑1 (MQ=255)
                                                                                               aCCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTccc                                                       >  2:2760096/1‑150 (MQ=255)
                                                                                                ccTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCg                                                      <  2:4065133/150‑1 (MQ=255)
                                                                                                ccTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCg                                                      <  2:3759252/150‑1 (MQ=255)
                                                                                                 cTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCgg                                                     <  2:1177483/150‑1 (MQ=255)
                                                                                                    cGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAt                                                  <  2:1005620/150‑1 (MQ=255)
                                                                                                          aCCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGcc                                            <  2:3008663/150‑1 (MQ=255)
                                                                                                           cccAGAATCCCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTCTATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCa                                           <  2:1494236/150‑1 (MQ=255)
                                                                                                                aaTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATccc                                      <  1:2931256/150‑1 (MQ=255)
                                                                                                                  tACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCAc                                    <  1:1422593/150‑1 (MQ=255)
                                                                                                                   aCCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCAcc                                   <  1:2078841/150‑1 (MQ=255)
                                                                                                                   aCCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCAcc                                   <  2:2272216/150‑1 (MQ=255)
                                                                                                                        tCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCa                              <  2:1430282/150‑1 (MQ=255)
                                                                                                                          ggCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAcc                            >  1:2145890/1‑150 (MQ=255)
                                                                                                                          ggCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAcc                            <  1:3526843/150‑1 (MQ=255)
                                                                                                                            cATCACCAGCAGCACCAGAATCAGCTGGGCGACTGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAccgc                          >  2:4413313/1‑150 (MQ=255)
                                                                                                                                 ccAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCgg                     >  1:1556802/1‑150 (MQ=255)
                                                                                                                                   agcagcACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTa                   <  1:4283247/150‑1 (MQ=255)
                                                                                                                                      agcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAg                >  1:24550/1‑150 (MQ=255)
                                                                                                                                      agcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAg                >  1:3284019/1‑150 (MQ=255)
                                                                                                                                      agcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAg                >  1:4136698/1‑150 (MQ=255)
                                                                                                                                       gcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAgg               >  2:76702/1‑150 (MQ=255)
                                                                                                                                          ccAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCtt            <  1:3238346/150‑1 (MQ=255)
                                                                                                                                           cAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCttt           <  2:1836037/150‑1 (MQ=255)
                                                                                                                                             gAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGGATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCa         <  1:3316863/150‑1 (MQ=255)
                                                                                                                                              aaTCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCAt        >  2:3657578/1‑150 (MQ=255)
                                                                                                                                                 cagcagGGCGAATGAGACCACATCTTTATAGTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATccc     <  2:4089817/150‑1 (MQ=255)
                                                                                                                                                  agcagGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCg    >  1:1736222/1‑150 (MQ=255)
                                                                                                                                                   gcagGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCgg   >  1:3776715/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGC  >  CP009273/3588755‑3589052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑