Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 329,887 G→T 10.7% I86I (ATC→ATA)  yahB ← putative DNA‑bindng transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273329,8870GT10.7% 782.4 / 43.8 242I86I (ATC→ATA) yahBputative DNA‑bindng transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (119/95);  new base T (17/11);  total (136/106)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCT  >  CP009273/329739‑330036
                                                                                                                                                    |                                                                                                                                                     
 gTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGAt                                                                                                                                                     >  2:761340/1‑150 (MQ=255)
  ttATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGAtt                                                                                                                                                    <  1:4058133/150‑1 (MQ=255)
    ataCACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCt                                                                                                                                                  <  2:1397079/150‑1 (MQ=255)
     taCACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTg                                                                                                                                                 >  1:263091/1‑150 (MQ=255)
      acacTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGc                                                                                                                                                <  1:1363502/150‑1 (MQ=255)
       cacTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCg                                                                                                                                               >  1:2530290/1‑150 (MQ=255)
         cTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGtt                                                                                                                                             >  1:880345/1‑150 (MQ=255)
                ggtgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGc                                                                                                                                      >  2:1957436/1‑150 (MQ=255)
                     cGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATaaa                                                                                                                                 >  2:4525069/1‑150 (MQ=255)
                     cGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATaaa                                                                                                                                 >  1:1014165/1‑150 (MQ=255)
                           tCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCAt                                                                                                                           <  2:1565808/150‑1 (MQ=255)
                            cTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCAtt                                                                                                                          <  1:3801659/150‑1 (MQ=255)
                             tGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTc                                                                                                                         <  2:492824/150‑1 (MQ=255)
                             tGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTATGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTc                                                                                                                         >  1:3671740/1‑150 (MQ=255)
                                 aGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTcagca                                                                                                                     >  2:169724/1‑150 (MQ=255)
                                 aGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTATGATGGCATAAAAATCATTcagca                                                                                                                     >  2:2355790/1‑150 (MQ=255)
                                     aGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTc                                                                                                                 <  1:1530047/150‑1 (MQ=255)
                                       gAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCgg                                                                                                               <  2:2787386/150‑1 (MQ=255)
                                        aaaCTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGt                                                                                                              <  2:3531485/150‑1 (MQ=255)
                                         aaCTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTa                                                                                                             >  1:2813204/1‑150 (MQ=255)
                                            tGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCt                                                                                                          >  2:935817/1‑150 (MQ=255)
                                              cTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCttt                                                                                                        >  1:2517330/1‑150 (MQ=255)
                                               tttttCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTg                                                                                                       >  1:4186257/1‑150 (MQ=255)
                                                 tttCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCg                                                                                                     >  1:946150/1‑150 (MQ=255)
                                                    cAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGaa                                                                                                  >  2:3037379/1‑150 (MQ=255)
                                                     aGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGaaa                                                                                                 >  1:2307247/1‑150 (MQ=255)
                                                       cACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGaaaaa                                                                                               >  2:3202644/1‑150 (MQ=255)
                                                            gTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAAc                                                                                          >  1:169502/1‑150 (MQ=255)
                                                             tAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACg                                                                                         <  2:325759/150‑1 (MQ=255)
                                                               aCAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGcc                                                                                       >  2:295877/1‑150 (MQ=255)
                                                                  cgCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCgg                                                                                    <  1:3778162/149‑1 (MQ=255)
                                                                        cGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCg                                                                              >  1:105667/1‑150 (MQ=255)
                                                                        cGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCg                                                                              >  1:106030/1‑150 (MQ=255)
                                                                          gtgtgtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCTTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGTAAAAAATAACGCCCGGACTCCGtt                                                                            <  1:1741603/150‑1 (MQ=255)
                                                                           tgtgtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTa                                                                           <  1:4478198/150‑1 (MQ=255)
                                                                            gtgtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTaa                                                                          >  2:1547316/1‑150 (MQ=255)
                                                                            gtgtTTGGGCGTATAAAGCAGCTGAAAAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTaa                                                                          >  2:526809/1‑150 (MQ=255)
                                                                              gtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACt                                                                        >  2:3074251/1‑150 (MQ=255)
                                                                              gtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACt                                                                        >  2:4447917/1‑150 (MQ=255)
                                                                                ttGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACCCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCa                                                                      <  2:2175324/150‑1 (MQ=255)
                                                                                ttGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCa                                                                      >  2:4233301/1‑150 (MQ=255)
                                                                                 tGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCaa                                                                     >  2:4229116/1‑150 (MQ=255)
                                                                                 tGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCaa                                                                     >  2:1594561/1‑150 (MQ=255)
                                                                                   ggCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCTGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAAtg                                                                   <  1:1370911/150‑1 (MQ=255)
                                                                                   ggCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCTGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAAtg                                                                   <  1:1370836/150‑1 (MQ=255)
                                                                                     cGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGGTGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAAtgct                                                                 >  2:4200620/1‑150 (MQ=255)
                                                                                     cGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAAtgct                                                                 <  1:2239465/150‑1 (MQ=255)
                                                                                      gTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAAtgctg                                                                <  2:3205089/150‑1 (MQ=255)
                                                                                        ataAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCg                                                              <  2:1389179/150‑1 (MQ=255)
                                                                                         taAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCgg                                                             <  2:3355681/150‑1 (MQ=255)
                                                                                         taAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCgg                                                             >  2:3697635/1‑150 (MQ=255)
                                                                                            agcagcTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTa                                                          <  1:3979702/150‑1 (MQ=255)
                                                                                               agcTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTg                                                       >  1:897199/1‑150 (MQ=255)
                                                                                                   gATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAg                                                   <  2:1453442/150‑1 (MQ=255)
                                                                                                    aTTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGt                                                  >  1:3172690/1‑150 (MQ=255)
                                                                                                    aTTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGt                                                  >  2:4009930/1‑150 (MQ=255)
                                                                                                      tAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGa                                                >  2:3292130/1‑150 (MQ=255)
                                                                                                      tAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGa                                                <  2:2054770/150‑1 (MQ=255)
                                                                                                      aaaTACAAATGCGCACACGCGCTTCTCTGCCCTGCGAAATAGTAGCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGa                                                <  2:4286919/149‑1 (MQ=255)
                                                                                                        aTACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAAc                                              >  1:2613435/1‑150 (MQ=255)
                                                                                                          aCAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAg                                            >  1:924947/1‑150 (MQ=255)
                                                                                                          aCAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAg                                            >  2:3032012/1‑150 (MQ=255)
                                                                                                          aCAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCGGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAg                                            <  1:1774231/150‑1 (MQ=255)
                                                                                                              aTGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGcacc                                        <  1:3037379/150‑1 (MQ=255)
                                                                                                                   cacaCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATc                                   >  2:1594711/1‑150 (MQ=255)
                                                                                                                    acacGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATcc                                  >  1:313220/1‑150 (MQ=255)
                                                                                                                    acacGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATcc                                  >  1:1940637/1‑150 (MQ=255)
                                                                                                                    acacGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATcc                                  >  1:4125746/1‑150 (MQ=255)
                                                                                                                     cacGCGCTTCTATGCCCTGCTAAATAGTATATATTCTGCGTTAGATGTCATAAACATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCa                                 <  2:3084672/150‑1 (MQ=255)
                                                                                                                     cacGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCa                                 >  1:1614627/1‑150 (MQ=255)
                                                                                                                      acGCGCTTCTATGCCCTGCTAAATATTATCTTTTCTGCTTTTGATGTCATAAAAATCATTCAGCTGGTGGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAg                                <  1:796584/150‑1 (MQ=255)
                                                                                                                         cgcTTCTATGCCCTGCTAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGccc                             <  1:253705/150‑1 (MQ=255)
                                                                                                                          gcTTCTTTGCCCTGCTAAATTGTATATATTCTGCGTTTGATGGCATAAAAATCATTCAGCTGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                            <  1:1957912/150‑1 (MQ=255)
                                                                                                                          gcTTCTTTGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCTGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                            <  1:132620/150‑1 (MQ=255)
                                                                                                                          gcTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                            <  2:2775351/150‑1 (MQ=255)
                                                                                                                          gcTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                            >  2:2061641/1‑150 (MQ=255)
                                                                                                                           cTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCg                           <  2:880345/150‑1 (MQ=255)
                                                                                                                            ttCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                          >  1:401089/1‑150 (MQ=255)
                                                                                                                            ttCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                          >  1:776906/1‑150 (MQ=255)
                                                                                                                            ttCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                          >  2:3871079/1‑150 (MQ=255)
                                                                                                                             tCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtc                         <  2:1349723/150‑1 (MQ=255)
                                                                                                                             tCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtc                         <  2:1350508/150‑1 (MQ=255)
                                                                                                                               tATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGAATAAAAATCATTCAGCAGGTCGGTAGATTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtctc                       <  1:4374640/150‑1 (MQ=255)
                                                                                                                                aTGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTcc                      >  1:3664888/1‑150 (MQ=255)
                                                                                                                                    ccTGCGAAATAGTATCTATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATAc                  >  1:568820/1‑150 (MQ=255)
                                                                                                                                    ccTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATAc                  <  2:2554991/150‑1 (MQ=255)
                                                                                                                                     cTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACg                 >  2:3473778/1‑150 (MQ=255)
                                                                                                                                     cTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACg                 >  2:4181450/1‑150 (MQ=255)
                                                                                                                                      tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGc                >  1:1865986/1‑150 (MQ=255)
                                                                                                                                        cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCtt              >  1:2489937/1‑150 (MQ=255)
                                                                                                                                         taaaTAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTa             <  2:1066339/149‑1 (MQ=255)
                                                                                                                                          aaaTAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa            >  1:46609/1‑150 (MQ=255)
                                                                                                                                               gTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtg       >  2:3056103/1‑150 (MQ=255)
                                                                                                                                                 aTCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTa     <  2:4225803/150‑1 (MQ=255)
                                                                                                                                                 aTCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTa     >  2:1774622/1‑150 (MQ=255)
                                                                                                                                                 aTCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTa     >  2:3893092/1‑150 (MQ=255)
                                                                                                                                                 aTCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTa     <  1:325718/150‑1 (MQ=255)
                                                                                                                                                  tCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAg    >  2:290513/1‑150 (MQ=255)
                                                                                                                                                   cGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAga   <  2:3671740/150‑2 (MQ=255)
                                                                                                                                                    gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCt  >  1:2363836/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCT  >  CP009273/329739‑330036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑