Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,802,238 A→T 10.7% A113A (GCA→GCT)  waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,802,2380AT10.7% 862.2 / 73.9 272A113A (GCA→GCT) waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base A (118/125);  new base T (18/11);  total (136/136)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 274 total aligned reads displayed.

AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTA  >  CP009273/3802091‑3802387
                                                                                                                                                   |                                                                                                                                                     
aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  1:3706854/150‑1 (MQ=255)
aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  1:4344613/150‑1 (MQ=255)
aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  2:1439130/150‑1 (MQ=255)
aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  2:3704005/150‑1 (MQ=255)
aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  1:1442252/150‑1 (MQ=255)
    ccGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAc                                                                                                                                                 >  1:2138958/1‑150 (MQ=255)
    ccGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAc                                                                                                                                                 >  2:467600/1‑150 (MQ=255)
        tGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCTATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGtt                                                                                                                                             >  2:22372/1‑150 (MQ=255)
         ggTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGttt                                                                                                                                            >  2:561068/1‑150 (MQ=255)
         ggTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGttt                                                                                                                                            <  2:1625391/150‑1 (MQ=255)
           tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTcc                                                                                                                                          <  1:2415577/150‑1 (MQ=255)
           tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTcc                                                                                                                                          >  1:2220730/1‑150 (MQ=255)
             cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTg                                                                                                                                        <  2:1090169/150‑1 (MQ=255)
             cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTg                                                                                                                                        <  2:68806/150‑1 (MQ=255)
                 cgcTGCGTCATCGTTATCCTGATTTACCTATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATa                                                                                                                                    <  2:4369427/150‑1 (MQ=255)
                  gcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaa                                                                                                                                   >  2:2997376/1‑150 (MQ=255)
                  gcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaa                                                                                                                                   >  2:2283807/1‑150 (MQ=255)
                  gcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaa                                                                                                                                   >  2:2281947/1‑150 (MQ=255)
                   cTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaaa                                                                                                                                  >  1:1190782/1‑150 (MQ=255)
                   cTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaaa                                                                                                                                  >  1:994096/1‑150 (MQ=255)
                    tGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAg                                                                                                                                 <  1:2122097/150‑1 (MQ=255)
                    tGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAg                                                                                                                                 >  2:3574607/1‑150 (MQ=255)
                      cGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTc                                                                                                                               <  2:1817535/150‑1 (MQ=255)
                          aTCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGAcc                                                                                                                           <  2:3314801/150‑1 (MQ=255)
                            cGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCt                                                                                                                         <  2:90001/150‑1 (MQ=255)
                            cGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCt                                                                                                                         <  2:4004422/150‑1 (MQ=255)
                            cGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCt                                                                                                                         <  2:898821/150‑1 (MQ=255)
                             gTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTa                                                                                                                        <  1:1326845/150‑1 (MQ=255)
                             gTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTa                                                                                                                        <  2:265357/150‑1 (MQ=255)
                                aTCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAAc                                                                                                                     >  2:492687/1‑150 (MQ=255)
                                aTCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAAc                                                                                                                     >  2:3106468/1‑150 (MQ=255)
                                aTCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGACCGATGCACTCCACCGTTTCCTGAATAAAGTCGACCCtcctc                                                                                                                     >  2:3158312/1‑146 (MQ=255)
                                  ccTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTg                                                                                                                   >  2:2668355/1‑150 (MQ=255)
                                   cTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTgg                                                                                                                  <  2:796089/150‑1 (MQ=255)
                                              aTTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATg                                                                                                       >  1:4459905/1‑150 (MQ=255)
                                               ttACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATgg                                                                                                      >  1:924196/1‑150 (MQ=255)
                                                tACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGa                                                                                                     <  1:838847/150‑1 (MQ=255)
                                                tACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGa                                                                                                     <  1:4292710/150‑1 (MQ=255)
                                                 aCCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGaa                                                                                                    <  2:255742/150‑1 (MQ=255)
                                                  ccGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGaaa                                                                                                   <  1:1807060/150‑1 (MQ=255)
                                                   cGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAAc                                                                                                  <  1:3036199/150‑1 (MQ=255)
                                                       acaacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGaa                                                                                              >  2:3882108/1‑150 (MQ=255)
                                                       acaacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGaa                                                                                              >  1:1004695/1‑150 (MQ=255)
                                                         aacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACt                                                                                            <  1:2405364/150‑1 (MQ=255)
                                                           ccATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAt                                                                                          >  1:3543200/1‑150 (MQ=255)
                                                           ccATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAt                                                                                          >  1:4404911/1‑150 (MQ=255)
                                                                aCGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCt                                                                                     <  2:620002/150‑1 (MQ=255)
                                                                   ccAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAAc                                                                                  >  2:1998961/1‑150 (MQ=255)
                                                                   ccAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAAc                                                                                  <  2:865726/150‑1 (MQ=255)
                                                                       ccGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGa                                                                              >  1:983372/1‑150 (MQ=255)
                                                                       ccGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGa                                                                              >  2:1888116/1‑150 (MQ=255)
                                                                        cGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGAt                                                                             >  2:14998/1‑150 (MQ=255)
                                                                         ggTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGAtt                                                                            >  2:4148869/1‑150 (MQ=255)
                                                                          gTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTg                                                                           >  1:3670420/1‑150 (MQ=255)
                                                                           ttCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTgc                                                                          <  1:3957971/150‑1 (MQ=255)
                                                                            tCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTgcg                                                                         <  1:245830/150‑1 (MQ=255)
                                                                             cGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACTGAACTATGGCCTAACCTGATTgcgg                                                                        >  1:3853017/1‑150 (MQ=255)
                                                                                    cgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACa                                                                 >  1:4452301/1‑150 (MQ=255)
                                                                                    cgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACa                                                                 <  1:2668355/150‑1 (MQ=255)
                                                                                    cgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACa                                                                 <  1:1843358/150‑1 (MQ=255)
                                                                                      tACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATa                                                               <  1:1510219/150‑1 (MQ=255)
                                                                                        cAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATaaa                                                             >  2:3304186/1‑150 (MQ=255)
                                                                                         aaTCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAAc                                                            <  2:2135997/150‑1 (MQ=255)
                                                                                          aTCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACg                                                           >  1:510211/1‑150 (MQ=255)
                                                                                          aTCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCACGATGCACTCAACAGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAACCg                                                           <  1:4105240/150‑1 (MQ=255)
                                                                                                tttCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAAt                                                     <  1:423580/150‑1 (MQ=255)
                                                                                                 ttCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAAtt                                                    >  1:1167004/1‑150 (MQ=255)
                                                                                                  tCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTc                                                   <  1:3311710/150‑1 (MQ=255)
                                                                                                   cGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTcc                                                  <  2:306247/150‑1 (MQ=255)
                                                                                                    gggAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCg                                                 >  2:322780/1‑150 (MQ=255)
                                                                                                        aGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTgg                                             >  1:2090429/1‑150 (MQ=255)
                                                                                                             gTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTTACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATc                                        <  2:2197049/150‑1 (MQ=255)
                                                                                                             gTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATc                                        >  2:2114176/1‑150 (MQ=255)
                                                                                                                cAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCt                                     <  2:4382875/150‑1 (MQ=255)
                                                                                                                 aGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTa                                    >  2:2765184/1‑150 (MQ=255)
                                                                                                                  gCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTaa                                   >  2:1254844/1‑150 (MQ=255)
                                                                                                                   cACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAc                                  >  1:910565/1‑150 (MQ=255)
                                                                                                                     cttttATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgc                                >  1:2824216/3‑150 (MQ=255)
                                                                                                                     cttttATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgc                                >  1:2824082/3‑150 (MQ=255)
                                                                                                                       tttATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgcgc                              <  1:4049956/150‑1 (MQ=255)
                                                                                                                           tCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGAct                          <  1:1098446/150‑1 (MQ=255)
                                                                                                                            cTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActc                         >  1:3623766/1‑150 (MQ=255)
                                                                                                                               ccGTATGATCTGCCCGATGGACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActctct                      <  2:2915394/150‑1 (MQ=255)
                                                                                                                               ccGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActctct                      <  2:635442/150‑1 (MQ=255)
                                                                                                                                 gTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGc                    >  1:3163434/1‑150 (MQ=255)
                                                                                                                                  tATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGcc                   >  1:112979/1‑150 (MQ=255)
                                                                                                                                  tATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGcc                   >  1:4522436/1‑150 (MQ=255)
                                                                                                                                    tGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCg                 >  1:1487754/1‑150 (MQ=255)
                                                                                                                                      aTCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCt               >  1:2784748/1‑150 (MQ=255)
                                                                                                                                      aTCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCt               <  2:3114227/150‑1 (MQ=255)
                                                                                                                                      aTCTGCCCGATGCACTCAACCGCTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCt               <  2:1744960/150‑1 (MQ=255)
                                                                                                                                       tCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc              <  2:3367426/150‑1 (MQ=255)
                                                                                                                                          gCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGc           >  1:3364185/1‑150 (MQ=255)
                                                                                                                                            ccGATGCACTCAACCGATTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCAGCTGGTGATCGCTAACTCGCGACTCTCTGCCCGCTCGGCCg         >  1:4272224/1‑150 (MQ=255)
                                                                                                                                              gATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCa       >  1:3403075/1‑150 (MQ=255)
                                                                                                                                               aTGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAg      <  2:101773/150‑1 (MQ=255)
                                                                                                                                               aTGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCGAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAg      <  2:2405270/150‑1 (MQ=255)
                                                                                                                                                 gCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTCCCCGCTCGGCCGCAGGt    >  2:2792995/1‑150 (MQ=255)
                                                                                                                                                  cACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGtt   <  1:3224530/150‑1 (MQ=255)
                                                                                                                                                   aCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTa  <  1:711910/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTA  >  CP009273/3802091‑3802387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑