Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,814,302 T→A 11.7% S77C (AGC→TGC)  ligB ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,814,3020TA11.7% 786.9 / 70.6 256S77C (AGC→TGC) ligBDNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base T (100/126);  new base A (24/6);  total (124/132)
Fisher's exact test for biased strand distribution p-value = 3.22e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

ATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTCTGGCTG  >  CP009273/3814153‑3814451
                                                                                                                                                     |                                                                                                                                                     
aTCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGgctcgct                                                                                                                                                       <  1:323040/150‑1 (MQ=255)
         tGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAagca                                                                                                                                              <  2:2940971/150‑1 (MQ=255)
         tGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAagca                                                                                                                                              <  1:281541/150‑1 (MQ=255)
          ggCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAagcag                                                                                                                                             <  1:1435208/150‑1 (MQ=255)
            cTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCg                                                                                                                                           >  2:4214385/1‑150 (MQ=255)
              gCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGtt                                                                                                                                         <  1:438577/150‑1 (MQ=255)
               cACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTg                                                                                                                                        <  1:1966404/150‑1 (MQ=255)
                 cccAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGcc                                                                                                                                      <  1:534833/150‑1 (MQ=255)
                    aaaGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACt                                                                                                                                   <  2:883222/150‑1 (MQ=255)
                    aaaGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACt                                                                                                                                   >  2:3442701/1‑150 (MQ=255)
                    aaaGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACt                                                                                                                                   <  1:924868/150‑1 (MQ=255)
                      aGATCGCTACGTTCTCGCATCCACAGTCTTAATGCGTTTTTATCCTCCATTTTGCGTACACCCTTATGGGCAAACGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGc                                                                                                                                 <  1:2448649/150‑1 (MQ=255)
                      aGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGc                                                                                                                                 <  1:3678690/150‑1 (MQ=255)
                       gATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCg                                                                                                                                >  2:40078/1‑150 (MQ=255)
                           gCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAg                                                                                                                            <  1:1703057/150‑1 (MQ=255)
                           gCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAg                                                                                                                            >  1:1070860/1‑150 (MQ=255)
                           gCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAg                                                                                                                            >  2:530522/1‑150 (MQ=255)
                            cTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGa                                                                                                                           >  2:963688/1‑150 (MQ=255)
                              aCGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACg                                                                                                                         <  1:633862/150‑1 (MQ=255)
                               cGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACgg                                                                                                                        >  1:4536707/1‑150 (MQ=255)
                               cGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACgg                                                                                                                        >  1:4536317/1‑150 (MQ=255)
                                   ctcGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACTGgcgc                                                                                                                    >  2:2001866/1‑150 (MQ=255)
                                   ctcGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGgcgc                                                                                                                    >  2:2001979/1‑150 (MQ=255)
                                    tcGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCt                                                                                                                   <  2:1578421/150‑1 (MQ=255)
                                       cATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTaa                                                                                                                >  2:52575/1‑150 (MQ=255)
                                         tCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACt                                                                                                              >  1:1267016/1‑150 (MQ=255)
                                           cacaGACTTAAGGCGTTTTTATCCACCATTTTGCGTCAACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACtta                                                                                                            >  2:4408012/1‑148 (MQ=255)
                                            acaGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGAt                                                                                                           <  2:1439868/150‑1 (MQ=255)
                                             caGAATTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATc                                                                                                          <  1:1525097/150‑1 (MQ=255)
                                               gACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGt                                                                                                        <  2:441516/150‑1 (MQ=255)
                                                   tAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAac                                                                                                    <  2:2785940/150‑1 (MQ=255)
                                                    aaTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAaca                                                                                                   >  2:1108891/1‑150 (MQ=255)
                                                     aTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAacac                                                                                                  >  2:4085569/1‑150 (MQ=255)
                                                      tGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCTCGGGGCTCGCACCCTAAGCAGCGTTGCCACTGCGTTAGACGGGCGCTTAACTGATCGTAAACAcc                                                                                                 <  1:2019845/150‑1 (MQ=255)
                                                      tGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACAcc                                                                                                 <  1:2865691/150‑1 (MQ=255)
                                                        cGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGt                                                                                               >  1:2294017/1‑150 (MQ=255)
                                                         gTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTc                                                                                              <  2:314986/150‑1 (MQ=255)
                                                         gTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTc                                                                                              >  1:2993636/1‑150 (MQ=255)
                                                            tttATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTc                                                                                           <  2:2662336/150‑1 (MQ=255)
                                                            tttATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTc                                                                                           <  1:2116223/150‑1 (MQ=255)
                                                            tttATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTc                                                                                           >  2:3419189/1‑150 (MQ=255)
                                                              tATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCa                                                                                         <  2:4110719/150‑1 (MQ=255)
                                                               aTCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCAc                                                                                        <  1:3818987/150‑1 (MQ=255)
                                                                 ccaccaTTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCt                                                                                      >  1:1284566/1‑150 (MQ=255)
                                                                    ccaTTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTCACTGATCGTAAACACCGTCTTCCACCTCAc                                                                                   >  1:4297169/1‑150 (MQ=255)
                                                                     caTTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACt                                                                                  >  1:3346964/1‑150 (MQ=255)
                                                                        tttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACtttt                                                                               <  1:2462258/150‑1 (MQ=255)
                                                                        tttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACtttt                                                                               <  1:1295948/150‑1 (MQ=255)
                                                                            cGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAATGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttcct                                                                           >  1:3699406/1‑150 (MQ=255)
                                                                             gTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttcctt                                                                          >  1:3260211/1‑150 (MQ=255)
                                                                             gTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttcctt                                                                          >  2:1342130/1‑150 (MQ=255)
                                                                             gTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttcctt                                                                          >  2:123192/1‑150 (MQ=255)
                                                                               acacCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttcc                                                                        <  1:217744/150‑1 (MQ=255)
                                                                               acacCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttcc                                                                        <  1:3440250/150‑1 (MQ=255)
                                                                                  cccGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttccttc                                                                     <  1:1120031/150‑1 (MQ=255)
                                                                                   ccGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttccttcc                                                                    <  1:3526285/150‑1 (MQ=255)
                                                                                     gTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCag                                                                  >  1:2963068/1‑150 (MQ=255)
                                                                                      tATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagt                                                                 <  1:1593624/150‑1 (MQ=255)
                                                                                       aTGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagta                                                                <  2:4458335/150‑1 (MQ=255)
                                                                                        tGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagtag                                                               <  2:1604151/150‑1 (MQ=255)
                                                                                        tGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagtag                                                               <  1:4125274/150‑1 (MQ=255)
                                                                                         gggCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagtagt                                                              <  2:722675/150‑1 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          <  2:2379616/150‑1 (MQ=255)
                                                                                                cGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTc                                                       >  2:3533539/1‑150 (MQ=255)
                                                                                                  gATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTccc                                                     <  1:3814625/150‑1 (MQ=255)
                                                                                                  gATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTccc                                                     >  2:3047227/1‑150 (MQ=255)
                                                                                                     gCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACt                                                  <  2:1703824/150‑1 (MQ=255)
                                                                                                        tAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGtt                                               <  2:3699846/150‑1 (MQ=255)
                                                                                                        tAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGtt                                               >  1:2061674/1‑150 (MQ=255)
                                                                                                         aaCCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGttt                                              >  1:2891587/1‑150 (MQ=255)
                                                                                                          aCCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGtttt                                             >  2:1398389/1‑150 (MQ=255)
                                                                                                           ccGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTa                                            <  1:457088/150‑1 (MQ=255)
                                                                                                            cGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAt                                           >  1:1826727/1‑150 (MQ=255)
                                                                                                             gCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAtt                                          <  1:2593858/150‑1 (MQ=255)
                                                                                                             gCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAtt                                          >  2:378773/1‑150 (MQ=255)
                                                                                                              cACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAttt                                         <  2:2310399/150‑1 (MQ=255)
                                                                                                                ccATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGc                                       >  2:3790796/1‑150 (MQ=255)
                                                                                                                     tAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTg                                  >  2:1694373/1‑150 (MQ=255)
                                                                                                                     tAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTg                                  <  2:1051937/150‑1 (MQ=255)
                                                                                                                       aaGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCa                                >  2:816530/1‑150 (MQ=255)
                                                                                                                        aGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAACGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAg                               <  2:3214986/150‑1 (MQ=255)
                                                                                                                         ggcggcATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAgg                              >  2:748980/1‑150 (MQ=255)
                                                                                                                            ggcATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCgg                           <  1:220624/150‑1 (MQ=255)
                                                                                                                              catcatCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGa                         >  2:2006464/1‑150 (MQ=255)
                                                                                                                               atcatcACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGaa                        <  1:392341/150‑1 (MQ=255)
                                                                                                                               atcatcACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGaa                        >  1:3151164/1‑150 (MQ=255)
                                                                                                                               atcatcACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGaa                        >  2:2110200/1‑150 (MQ=255)
                                                                                                                                tcatcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGaaa                       >  1:1215347/1‑150 (MQ=255)
                                                                                                                                 catcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAAt                      >  2:609948/1‑150 (MQ=255)
                                                                                                                                 catcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAAt                      >  1:1556176/1‑150 (MQ=255)
                                                                                                                                  atcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAAtt                     <  2:2432835/150‑1 (MQ=255)
                                                                                                                                  atcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAAtt                     <  2:1499362/150‑1 (MQ=255)
                                                                                                                                     aCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATttct                  <  2:3913299/150‑1 (MQ=255)
                                                                                                                                      cGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATttctt                 >  2:2632540/1‑150 (MQ=255)
                                                                                                                                         cgcgGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCt              >  1:4118032/1‑150 (MQ=255)
                                                                                                                                                 tcgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctg      >  2:2701417/1‑150 (MQ=255)
                                                                                                                                                 tcgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctg      >  2:2701376/1‑150 (MQ=255)
                                                                                                                                                 tcgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctg      <  2:2550660/150‑1 (MQ=255)
                                                                                                                                                  cgcacCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctgg     >  1:2651139/1‑150 (MQ=255)
                                                                                                                                                    ctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctggct   >  2:4298373/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTCTGGCTG  >  CP009273/3814153‑3814451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑