Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,816,686 G→T 47.3% S309S (TCG→TCT)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,816,6860GT47.3% 171.5 / inf 274S309S (TCG→TCT) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (65/79);  new base T (53/77);  total (118/156)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

ATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAT  >  CP009273/3816537‑3816835
                                                                                                                                                     |                                                                                                                                                     
aTCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                                                                                                                       <  1:2251145/150‑1 (MQ=255)
    aCGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGAGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAGCGGCTATCAGTCTTTGCACACCTCgatga                                                                                                                                                   <  2:3151188/150‑1 (MQ=255)
      gCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATc                                                                                                                                                 <  1:1909623/150‑1 (MQ=255)
       cTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATAGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCg                                                                                                                                                >  2:3459667/1‑150 (MQ=255)
         ttCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                                                                                                              >  1:1404676/1‑150 (MQ=255)
            cgcgTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCg                                                                                                                                           >  1:1902392/1‑150 (MQ=255)
              cgTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCa                                                                                                                                         <  2:1045667/150‑1 (MQ=255)
               gTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc                                                                                                                                        >  2:4032242/1‑150 (MQ=255)
                tGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACg                                                                                                                                       >  2:3022796/1‑150 (MQ=255)
                  aTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGgt                                                                                                                                     <  2:2315188/150‑1 (MQ=255)
                  aTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgt                                                                                                                                     >  1:1726063/1‑150 (MQ=255)
                     gTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGtgtg                                                                                                                                  <  1:1377106/150‑1 (MQ=255)
                     gTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGtgtg                                                                                                                                  <  1:2510542/150‑1 (MQ=255)
                          tGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGt                                                                                                                             >  1:3880945/1‑150 (MQ=255)
                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGtt                                                                                                                            >  1:1124787/1‑150 (MQ=255)
                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGtt                                                                                                                            >  1:265930/1‑150 (MQ=255)
                            aTTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTg                                                                                                                           >  1:203528/1‑150 (MQ=255)
                             ttCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGa                                                                                                                          >  2:2813149/1‑150 (MQ=255)
                             ttCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGa                                                                                                                          <  2:2931966/150‑1 (MQ=255)
                              tCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAg                                                                                                                         >  2:4481189/1‑150 (MQ=255)
                              tCTGACACCTGTTAGCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAg                                                                                                                         <  2:3780910/150‑1 (MQ=255)
                                   cacCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCa                                                                                                                    <  1:1253899/150‑1 (MQ=255)
                                        gTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                                                                                                               <  2:1247737/150‑1 (MQ=255)
                                           aTCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTa                                                                                                            >  1:2195752/1‑150 (MQ=255)
                                            tCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGACCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTAc                                                                                                           >  1:2672790/1‑150 (MQ=255)
                                             cgcgTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTAcc                                                                                                          <  2:4243037/150‑1 (MQ=255)
                                              gcgTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCg                                                                                                         <  1:637152/150‑1 (MQ=255)
                                               cgTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGa                                                                                                        >  2:2499094/1‑150 (MQ=255)
                                                    tGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGata                                                                                                   <  2:3452770/150‑1 (MQ=255)
                                                     gggCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGatat                                                                                                  >  1:4233527/1‑150 (MQ=255)
                                                         cAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAc                                                                                              <  1:921368/150‑1 (MQ=255)
                                                          aGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAcc                                                                                             >  2:1013209/1‑150 (MQ=255)
                                                           gATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCa                                                                                            >  1:167129/1‑150 (MQ=255)
                                                                 caGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGc                                                                                      >  2:870245/1‑150 (MQ=255)
                                                                      tGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGgaga                                                                                 <  1:741128/150‑1 (MQ=255)
                                                                          cAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATggg                                                                             <  2:1804874/150‑1 (MQ=255)
                                                                           aaGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgt                                                                            <  1:1055736/150‑1 (MQ=255)
                                                                            aGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg                                                                           <  1:3179509/150‑1 (MQ=255)
                                                                                  gTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcg                                                                     >  1:3946500/1‑150 (MQ=255)
                                                                                  gTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcg                                                                     >  1:1167270/1‑150 (MQ=255)
                                                                                   tCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcgc                                                                    <  2:2418938/150‑1 (MQ=255)
                                                                                   tCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcgc                                                                    <  2:1472461/150‑1 (MQ=255)
                                                                                   tCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcgc                                                                    >  2:3858286/1‑150 (MQ=255)
                                                                                    ccGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcgcg                                                                   <  2:2181800/150‑1 (MQ=255)
                                                                                     cGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCgcgc                                                                  >  1:843458/1‑150 (MQ=255)
                                                                                     cGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCgcgc                                                                  >  1:3398727/1‑150 (MQ=255)
                                                                                       ggCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCAc                                                                <  2:363723/150‑1 (MQ=255)
                                                                                       ggCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCAc                                                                >  2:1125777/1‑150 (MQ=255)
                                                                                       ggCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCAc                                                                >  2:926485/1‑150 (MQ=255)
                                                                                        gCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACt                                                               <  2:2059009/150‑1 (MQ=255)
                                                                                             gTGAAAGACTATATCGCCATTCCAAAAGCGTACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCt                                                          <  1:526946/150‑1 (MQ=255)
                                                                                             gTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCt                                                          >  1:181160/1‑150 (MQ=255)
                                                                                              tGAAAGACTATATCGCCAGTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCtt                                                         <  1:1438175/150‑1 (MQ=255)
                                                                                                aaaGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTtat                                                       >  1:3288632/1‑150 (MQ=255)
                                                                                                aaaGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTtat                                                       <  2:3510337/150‑1 (MQ=255)
                                                                                                aaaGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATACGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTtat                                                       >  2:389264/1‑150 (MQ=255)
                                                                                                  aGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                                                     <  1:883610/150‑1 (MQ=255)
                                                                                                  aGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                                                     <  1:883672/150‑1 (MQ=255)
                                                                                                  aGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                                                     >  2:2480658/1‑150 (MQ=255)
                                                                                                  aGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                                                     <  1:498012/150‑1 (MQ=255)
                                                                                                   gACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaaa                                                    <  2:3184363/150‑1 (MQ=255)
                                                                                                   gACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaaa                                                    <  2:958658/150‑1 (MQ=255)
                                                                                                   gACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaaa                                                    <  1:3971914/150‑1 (MQ=255)
                                                                                                    aCTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAag                                                   >  1:3681650/1‑150 (MQ=255)
                                                                                                      tataTCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAagag                                                 >  2:2588738/1‑150 (MQ=255)
                                                                                                      tataTCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAagag                                                 >  1:737545/1‑150 (MQ=255)
                                                                                                        tatCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCa                                               <  2:1307660/150‑1 (MQ=255)
                                                                                                        tatCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCa                                               >  1:2863634/1‑150 (MQ=255)
                                                                                                         atCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCAc                                              <  2:1988382/150‑1 (MQ=255)
                                                                                                         atCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCAc                                              <  2:1671506/150‑1 (MQ=255)
                                                                                                           cGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACgg                                            <  1:3337076/150‑1 (MQ=255)
                                                                                                             ccATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCg                                          >  2:3386191/1‑150 (MQ=255)
                                                                                                              cATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGa                                         <  1:3124825/150‑1 (MQ=255)
                                                                                                                ttCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGaaa                                       >  1:1638509/1‑150 (MQ=255)
                                                                                                                  ccAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAcc                                     <  1:3548244/150‑1 (MQ=255)
                                                                                                                    aaaaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAg                                   >  2:670031/1‑150 (MQ=255)
                                                                                                                    aaaaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAg                                   <  1:3645840/150‑1 (MQ=255)
                                                                                                                     aaaGCGAACGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGt                                  <  2:2099963/150‑1 (MQ=255)
                                                                                                                       aGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtac                                <  1:3022876/150‑1 (MQ=255)
                                                                                                                         cGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtacta                              <  1:3259583/150‑1 (MQ=255)
                                                                                                                           aaCGGCTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTAcc                            <  1:2293362/150‑1 (MQ=255)
                                                                                                                                cTATCAGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGAACTACCGcaca                       >  1:2338027/1‑150 (MQ=255)
                                                                                                                                cTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGcaca                       <  1:831988/150‑1 (MQ=255)
                                                                                                                                cTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGcaca                       <  1:3833573/150‑1 (MQ=255)
                                                                                                                                   tCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAAt                    <  1:4121036/150‑1 (MQ=255)
                                                                                                                                   tCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAAt                    <  1:1871119/150‑1 (MQ=255)
                                                                                                                                    cAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATc                   <  1:4279968/150‑1 (MQ=255)
                                                                                                                                    cAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATc                   <  1:3158582/150‑1 (MQ=255)
                                                                                                                                     aGTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATcc                  <  2:203528/150‑1 (MQ=255)
                                                                                                                                     aGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATcc                  <  2:3967760/150‑1 (MQ=255)
                                                                                                                                      gTCTTTGCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcg                 <  2:265930/150‑1 (MQ=255)
                                                                                                                                       tCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcgc                <  2:267033/150‑1 (MQ=255)
                                                                                                                                          ttGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGcc             <  1:1291662/150‑1 (MQ=255)
                                                                                                                                           tGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGccc            <  1:1949103/150‑1 (MQ=255)
                                                                                                                                            gCACACCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCa           <  1:4110266/150‑1 (MQ=255)
                                                                                                                                             cacaCCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAg          >  1:4491071/1‑150 (MQ=255)
                                                                                                                                               cacCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAgcg        <  1:4397978/150‑1 (MQ=255)
                                                                                                                                               cacCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAgcg        >  2:1670179/1‑150 (MQ=255)
                                                                                                                                               cacCTCTATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAgcg        >  2:1587598/1‑150 (MQ=255)
                                                                                                                                                  cTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTg     <  1:702313/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAT  >  CP009273/3816537‑3816835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑