Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,829,715 T→G 10.7% intergenic (+39/‑70) selC → / → yicT tRNA‑Sec/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,829,7150TG10.7% 690.4 / 78.3 262intergenic (+39/‑70)selC/yicTtRNA‑Sec/pseudogene
Reads supporting (aligned to +/- strand):  ref base T (127/107);  new base G (14/14);  total (141/121)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 272 total aligned reads displayed.

GTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCG  >  CP009273/3829566‑3829864
                                                                                                                                                     |                                                                                                                                                     
gTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACt                                                                                                                                                       <  2:1226598/150‑1 (MQ=255)
  aGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGt                                                                                                                                                     >  2:2306468/1‑150 (MQ=255)
    cAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCa                                                                                                                                                   <  2:3117426/150‑1 (MQ=255)
      aTCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAAt                                                                                                                                                 >  2:2754712/1‑150 (MQ=255)
         gAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCa                                                                                                                                              >  1:4070709/1‑150 (MQ=255)
          aGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAg                                                                                                                                             >  1:4381629/1‑150 (MQ=255)
            gcgcGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGat                                                                                                                                           <  1:3875962/150‑1 (MQ=255)
              gcgGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGatat                                                                                                                                         <  1:3675971/150‑1 (MQ=255)
                 gAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATAtttt                                                                                                                                      <  2:3233331/150‑1 (MQ=255)
                    gATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTa                                                                                                                                   >  2:4363326/1‑150 (MQ=255)
                    gATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTa                                                                                                                                   >  2:3617171/1‑150 (MQ=255)
                       cgtcgtCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTg                                                                                                                                >  1:1676070/1‑150 (MQ=255)
                       cgtcgtCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTg                                                                                                                                >  1:1212530/1‑150 (MQ=255)
                       cgtcgtCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTg                                                                                                                                >  1:2468226/1‑150 (MQ=255)
                        gtcgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGc                                                                                                                               >  1:116162/1‑150 (MQ=255)
                         tcgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCt                                                                                                                              <  2:2589362/150‑1 (MQ=255)
                          cgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTc                                                                                                                             <  1:1091037/150‑1 (MQ=255)
                           gtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCa                                                                                                                            >  2:707504/1‑150 (MQ=255)
                            tctcCGGGGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCaa                                                                                                                           <  1:4410753/150‑1 (MQ=255)
                             ctcCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAat                                                                                                                          <  2:4416689/150‑1 (MQ=255)
                             ctcCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAat                                                                                                                          >  2:3967383/1‑150 (MQ=255)
                               ccGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAatat                                                                                                                        <  2:1256352/150‑1 (MQ=255)
                                 ggTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTc                                                                                                                      <  1:538592/150‑1 (MQ=255)
                                 ggTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTc                                                                                                                      <  1:3130207/150‑1 (MQ=255)
                                   tGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCaa                                                                                                                    >  1:1796570/1‑150 (MQ=255)
                                        cggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGc                                                                                                               >  1:1489075/1‑150 (MQ=255)
                                        cggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGc                                                                                                               >  1:1974557/1‑150 (MQ=255)
                                          gcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCtt                                                                                                             <  2:3309217/150‑1 (MQ=255)
                                          gcTGGACGTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTCCTACTGCTCAATATTCAATCGGCtt                                                                                                             <  2:2704490/150‑1 (MQ=255)
                                           cTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGGTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTc                                                                                                            <  2:4149246/150‑1 (MQ=255)
                                            tGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCa                                                                                                           >  2:4452605/1‑150 (MQ=255)
                                               aCTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTTATCTTCCGCCAAAATGACTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGa                                                                                                        <  1:3598558/150‑1 (MQ=255)
                                               aCTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGa                                                                                                        <  1:3599341/150‑1 (MQ=255)
                                               aCTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGa                                                                                                        <  1:2998358/150‑1 (MQ=255)
                                                 ttCATATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCTACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAAt                                                                                                      <  2:3895937/150‑1 (MQ=255)
                                                   cAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATAc                                                                                                    >  1:1545374/1‑150 (MQ=255)
                                                     aaTCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCt                                                                                                  >  1:1190861/1‑150 (MQ=255)
                                                      aTCCAGTTGGGGCCGCCTGCGGTCCCGGTCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGACTCTCCTGACTTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCTGCTTCAGGAATACCTg                                                                                                 <  2:454306/150‑1 (MQ=255)
                                                         cAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTc                                                                                              >  1:8993/1‑150 (MQ=255)
                                                         cAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTc                                                                                              >  1:8970/1‑150 (MQ=255)
                                                         cAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTc                                                                                              >  1:9051/1‑150 (MQ=255)
                                                           gTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTa                                                                                            >  1:3396489/1‑150 (MQ=255)
                                                            ttGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTaa                                                                                           <  1:4444528/150‑1 (MQ=255)
                                                               gggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGt                                                                                        >  1:2407980/1‑150 (MQ=255)
                                                               gggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGt                                                                                        <  1:953507/150‑1 (MQ=255)
                                                                ggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGtt                                                                                       <  2:164532/150‑1 (MQ=255)
                                                                    gccAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTtgt                                                                                   >  1:4344036/1‑150 (MQ=255)
                                                                     ccAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTtgtg                                                                                  >  1:3497030/1‑150 (MQ=255)
                                                                         cGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATgg                                                                              >  2:329835/1‑150 (MQ=255)
                                                                           gTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGAc                                                                            <  2:4070709/150‑1 (MQ=255)
                                                                           gTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGAc                                                                            >  1:2167992/1‑150 (MQ=255)
                                                                              ccGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGAt                                                                         <  2:193041/150‑1 (MQ=255)
                                                                              ccGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGAt                                                                         <  2:196392/150‑1 (MQ=255)
                                                                              ccGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGAt                                                                         <  1:1958734/150‑1 (MQ=255)
                                                                                gggCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGa                                                                       >  1:566225/1‑150 (MQ=255)
                                                                                gggCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGa                                                                       <  2:3401932/150‑1 (MQ=255)
                                                                                    aGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCgg                                                                   >  1:4392045/1‑150 (MQ=255)
                                                                                       ttCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCAGCTg                                                                >  1:1306986/1‑150 (MQ=255)
                                                                                       ttCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCCTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTg                                                                >  1:1600108/1‑150 (MQ=255)
                                                                                         cGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaa                                                              >  2:4199315/1‑150 (MQ=255)
                                                                                          gACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaag                                                             >  2:699087/1‑150 (MQ=255)
                                                                                            cTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTATGTGATGGACGATGAGCGGCTgaagaa                                                           >  2:2039521/1‑150 (MQ=255)
                                                                                              ccTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAAACCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCGGGTCTAAGGGGTTGTGATGGACGATGAGCGGCTGTAGAATc                                                         >  1:3085617/1‑150 (MQ=255)
                                                                                               cTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATcc                                                        >  1:1252527/1‑150 (MQ=255)
                                                                                               cTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATcc                                                        >  1:867554/1‑150 (MQ=255)
                                                                                                tgtgATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCg                                                       <  2:209259/150‑1 (MQ=255)
                                                                                                  tgATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGcc                                                     >  2:367966/1‑150 (MQ=255)
                                                                                                   gATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCt                                                    >  2:13083/1‑150 (MQ=255)
                                                                                                    aTCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTg                                                   <  1:163569/150‑1 (MQ=255)
                                                                                                    aTCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTg                                                   <  1:3372106/150‑1 (MQ=255)
                                                                                                     tCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGt                                                  >  2:869746/1‑150 (MQ=255)
                                                                                                     tCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGt                                                  <  1:2312391/150‑1 (MQ=255)
                                                                                                      cTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTc                                                 <  2:2241671/150‑1 (MQ=255)
                                                                                                        tCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCgg                                               <  1:2654644/150‑1 (MQ=255)
                                                                                                                   gCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTcc                                    >  2:2546254/1‑150 (MQ=255)
                                                                                                                   gCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTGTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTcc                                    >  2:1501058/1‑150 (MQ=255)
                                                                                                                    ccTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCt                                   <  1:697368/150‑1 (MQ=255)
                                                                                                                        tcCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGaa                               <  1:3973521/150‑1 (MQ=255)
                                                                                                                          cTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACg                             >  1:1320508/1‑150 (MQ=255)
                                                                                                                          cTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACg                             >  1:2591983/1‑150 (MQ=255)
                                                                                                                            gACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCa                           <  2:493930/150‑1 (MQ=255)
                                                                                                                            gACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCa                           >  2:3391237/1‑150 (MQ=255)
                                                                                                                              cGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATc                         <  1:2306468/150‑1 (MQ=255)
                                                                                                                               gTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATcc                        <  2:2060029/150‑1 (MQ=255)
                                                                                                                               gTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATcc                        <  1:377351/150‑1 (MQ=255)
                                                                                                                                 tctcCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCcgc                      >  1:2985310/1‑150 (MQ=255)
                                                                                                                                  ctcCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCcgcg                     <  2:1678050/150‑1 (MQ=255)
                                                                                                                                  ctcCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCcgcg                     <  2:3522008/150‑1 (MQ=255)
                                                                                                                                    ccAACCCCTGGTAATACTGTCAATCCAGATTTTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTTGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTATTCTGTCCTCGAACGCATCCGCGat                   <  1:452882/150‑1 (MQ=255)
                                                                                                                                      aaCCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGatat                 >  1:3131433/1‑150 (MQ=255)
                                                                                                                                        ccccTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATAGGCTACAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTc               <  2:2908924/150‑1 (MQ=255)
                                                                                                                                                aaTACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGt       <  1:1753403/150‑1 (MQ=255)
                                                                                                                                                 aTACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTa      >  2:2505515/1‑150 (MQ=255)
                                                                                                                                                  tACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTAt     >  2:2412000/1‑150 (MQ=255)
                                                                                                                                                   aCTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATg    >  1:2012817/1‑150 (MQ=255)
                                                                                                                                                   aCTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATg    >  2:2079313/1‑150 (MQ=255)
                                                                                                                                                   aCTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATg    >  1:4263404/1‑150 (MQ=255)
                                                                                                                                                     tGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgcg  >  1:3163992/1‑150 (MQ=255)
                                                                                                                                                     tGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgcg  <  1:278892/150‑1 (MQ=255)
                                                                                                                                                     tGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgcg  >  1:3154320/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCG  >  CP009273/3829566‑3829864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑