BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,891,652	G→T	12.3%	E91* (GAA→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,891,652	0	G	T	12.3%	950.5 / 73.6	283	E91* (GAA→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base G (129/119); new base T (11/24); total (140/143)
Fisher's exact test for biased strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 289 total aligned reads displayed.

ATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGAT  >  CP009273/3891503‑3891801

attaCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACg                                                                                                                                                       <  1:2772792/150‑1 (MQ=255)

attaCTCGGGTCCCTTGTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACg                                                                                                                                                       <  2:3675363/150‑1 (MQ=255)

  taCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGaa                                                                                                                                                     >  1:3182915/1‑150 (MQ=255)

    cTCGGGTCCCTTTTATTGCGTTGATGAAGTTTAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAtt                                                                                                                                                   <  2:4340419/150‑1 (MQ=255)

    cTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAtt                                                                                                                                                   >  1:3318594/1‑150 (MQ=255)

     tCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCTGACGGTTCTGCTGCTGAAAAGTTTGAAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAttt                                                                                                                                                  <  1:3757196/150‑1 (MQ=255)

     tCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAttt                                                                                                                                                  <  1:4083856/150‑1 (MQ=255)

     tCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAttt                                                                                                                                                  <  2:2558178/150‑1 (MQ=255)

      cGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTg                                                                                                                                                 >  1:984729/1‑150 (MQ=255)

        ggTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGcc                                                                                                                                               <  1:4139097/150‑1 (MQ=255)

        ggTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGcc                                                                                                                                               <  1:4509118/150‑1 (MQ=255)

            ccTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTa                                                                                                                                           >  2:4446027/1‑150 (MQ=255)

               tttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTa                                                                                                                                        <  2:3017185/150‑1 (MQ=255)

               tttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTa                                                                                                                                        <  1:2496941/150‑1 (MQ=255)

                ttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTaa                                                                                                                                       >  2:3835182/1‑150 (MQ=255)

                  aTTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGa                                                                                                                                     >  2:106074/1‑150 (MQ=255)

                        ttGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACat                                                                                                                               >  1:821489/1‑150 (MQ=255)

                          gatgaAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACatat                                                                                                                             >  1:813300/1‑150 (MQ=255)

                            tgaAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATcc                                                                                                                           <  1:4420706/150‑1 (MQ=255)

                                   gAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGaa                                                                                                                    >  1:3504282/1‑150 (MQ=255)

                                    aaCATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCTGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGaaa                                                                                                                   >  1:114631/1‑150 (MQ=255)

                                     aCATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAAt                                                                                                                  <  1:2931645/150‑1 (MQ=255)

                                        tctcTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCt                                                                                                               <  1:3187444/150‑1 (MQ=255)

                                        tctcTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCt                                                                                                               <  1:3781442/150‑1 (MQ=255)

                                         ctctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTg                                                                                                              <  2:814480/150‑1 (MQ=255)

                                          tctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGa                                                                                                             >  1:2293169/1‑150 (MQ=255)

                                          tctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGa                                                                                                             >  2:698549/1‑150 (MQ=255)

                                          tctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGa                                                                                                             >  2:1192224/1‑150 (MQ=255)

                                            tGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGaaa                                                                                                           <  1:2850767/150‑1 (MQ=255)

                                               ccGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAagag                                                                                                        <  2:664081/150‑1 (MQ=255)

                                                cGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgt                                                                                                       <  2:1502960/150‑1 (MQ=255)

                                                cGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgt                                                                                                       <  2:834813/150‑1 (MQ=255)

                                                 gtggtgTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgtt                                                                                                      >  1:1679115/1‑150 (MQ=255)

                                                  tggtgTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttg                                                                                                     <  1:621047/150‑1 (MQ=255)

                                                     tgtATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggtt                                                                                                  >  1:2825504/1‑150 (MQ=255)

                                                     tgtATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggtt                                                                                                  <  1:2708379/150‑1 (MQ=255)

                                                       tATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGa                                                                                                >  1:1248326/1‑150 (MQ=255)

                                                              gtcGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAacgcacg                                                                                         <  2:2667833/148‑1 (MQ=255)

                                                                 ggTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCa                                                                                      >  1:3031189/1‑150 (MQ=255)

                                                                 ggTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCa                                                                                      >  2:1196480/1‑150 (MQ=255)

                                                                 ggTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCa                                                                                      <  1:2179678/150‑1 (MQ=255)

                                                                   ttCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAgg                                                                                    >  1:1545588/1‑150 (MQ=255)

                                                                     ctgctgctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGtt                                                                                  >  2:2644930/1‑150 (MQ=255)

                                                                       gctgctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTAt                                                                                >  1:2377308/1‑150 (MQ=255)

                                                                        ctgctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTACTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATc                                                                               <  2:2344138/150‑1 (MQ=255)

                                                                        ctgctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATc                                                                               >  1:2819653/1‑150 (MQ=255)

                                                                          gctgAAATGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCat                                                                             <  2:2952819/150‑1 (MQ=255)

                                                                           ctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatg                                                                            <  1:1406163/150‑1 (MQ=255)

                                                                           ctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatg                                                                            <  1:1289257/150‑1 (MQ=255)

                                                                            tgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatgg                                                                           >  2:1982105/1‑150 (MQ=255)

                                                                            tgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatgg                                                                           >  2:4232499/1‑150 (MQ=255)

                                                                              aaaaGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatggat                                                                         <  1:2278096/150‑1 (MQ=255)

                                                                               aaaGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatggatg                                                                        >  2:2146808/1‑150 (MQ=255)

                                                                                        aGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGa                                                               <  2:4131832/150‑1 (MQ=255)

                                                                                          gTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAt                                                             >  2:3451504/1‑150 (MQ=255)

                                                                                           tAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                                                            <  1:1941690/150‑1 (MQ=255)

                                                                                           tAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                                                            <  1:3763889/150‑1 (MQ=255)

                                                                                           tAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                                                            <  2:824706/150‑1 (MQ=255)

                                                                                             gTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCt                                                          <  2:1590467/150‑1 (MQ=255)

                                                                                             gTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCt                                                          <  2:523067/150‑1 (MQ=255)

                                                                                               tttCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTgg                                                        >  2:3724484/1‑150 (MQ=255)

                                                                                                  cAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTc                                                     <  2:3381766/150‑1 (MQ=255)

                                                                                                   aGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcg                                                    >  2:4176378/1‑150 (MQ=255)

                                                                                                    ggATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcgc                                                   >  1:3247633/1‑150 (MQ=255)

                                                                                                     gatgatGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcgcg                                                  >  1:3616604/1‑150 (MQ=255)

                                                                                                        gatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAt                                               <  1:4212934/150‑1 (MQ=255)

                                                                                                        gatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAt                                               <  1:4212247/150‑1 (MQ=255)

                                                                                                         atgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAtt                                              >  1:2374125/1‑150 (MQ=255)

                                                                                                         atgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAtt                                              >  1:68809/1‑150 (MQ=255)

                                                                                                         atgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAtt                                              >  1:639687/1‑150 (MQ=255)

                                                                                                           gCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATTGCAGCAGGAATTCTGGCTCGCGCATtgt                                            >  2:309350/1‑150 (MQ=255)

                                                                                                            ccAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATtgtg                                           >  1:546556/1‑150 (MQ=255)

                                                                                                             cAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTgg                                          >  1:1040751/1‑150 (MQ=255)

                                                                                                             cAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTgg                                          <  1:4382443/150‑1 (MQ=255)

                                                                                                             cAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCAGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTgg                                          >  1:4463492/1‑150 (MQ=255)

                                                                                                              aGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGc                                         >  2:2950016/1‑150 (MQ=255)

                                                                                                               gCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCg                                        <  1:4180622/150‑1 (MQ=255)

                                                                                                                 aTAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGAt                                      >  1:2268680/1‑150 (MQ=255)

                                                                                                                     aaGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCt                                  >  1:1241586/1‑150 (MQ=255)

                                                                                                                            tGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGtttt                           <  2:2105653/150‑1 (MQ=255)

                                                                                                                            tGAATTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGtttt                           >  1:3806207/1‑150 (MQ=255)

                                                                                                                            tGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGtttt                           <  2:1724878/150‑1 (MQ=255)

                                                                                                                              aaTTTGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTa                         <  2:1247841/150‑1 (MQ=255)

                                                                                                                              aaTTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTa                         >  1:2657107/1‑150 (MQ=255)

                                                                                                                              aaTTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTa                         <  1:1695876/150‑1 (MQ=255)

                                                                                                                                tttGAGTATGATGAAGAGGACTAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATc                       <  1:2508480/150‑1 (MQ=255)

                                                                                                                                     gTATGATGTAGATGACGAATTTGCCGGTATTAAGAACACATATCATGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTa                  <  2:2249102/150‑1 (MQ=255)

                                                                                                                                      tATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGTTGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTAt                 <  1:3667847/150‑1 (MQ=255)

                                                                                                                                      tATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTAt                 >  2:3070575/1‑150 (MQ=255)

                                                                                                                                      tATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTAt                 <  1:922406/150‑1 (MQ=255)

                                                                                                                                         gatgaAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTAtgtg              >  2:4196393/1‑150 (MQ=255)

                                                                                                                                         gatgaAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTAtgtg              <  1:3137295/150‑1 (MQ=255)

                                                                                                                                            gaAGAGGACGAATTTGCCGGCATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGc           >  1:801899/1‑150 (MQ=255)

                                                                                                                                             aaGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCt          <  2:2828332/150‑1 (MQ=255)

                                                                                                                                             aaGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCt          <  1:3575508/150‑1 (MQ=255)

                                                                                                                                              agagGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTg         <  2:1540760/150‑1 (MQ=255)

                                                                                                                                              agagGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTg         >  1:3944557/1‑150 (MQ=255)

                                                                                                                                              agagGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTg         >  2:946918/1‑150 (MQ=255)

                                                                                                                                                 ggACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGaa      >  2:3129150/1‑150 (MQ=255)

                                                                                                                                                    cGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGa   >  2:439591/1‑150 (MQ=255)

ATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGAT  >  CP009273/3891503‑3891801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`