Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,893,385 C→A 10.7% D185Y (GAT→TAT)  yieL ← putative xylanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,893,3850CA10.7% 1021.7 / 63.3 289D185Y (GAT→TAT) yieLputative xylanase
Reads supporting (aligned to +/- strand):  ref base C (141/117);  new base A (15/16);  total (156/133)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 295 total aligned reads displayed.

CTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCG  >  CP009273/3893236‑3893534
                                                                                                                                                     |                                                                                                                                                     
cTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATc                                                                                                                                                       >  2:1256603/1‑150 (MQ=255)
cTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATc                                                                                                                                                       <  1:3188502/150‑1 (MQ=255)
 tGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCa                                                                                                                                                      >  2:1716023/1‑150 (MQ=255)
   aGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCAcc                                                                                                                                                    >  2:104441/1‑150 (MQ=255)
   aGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCAcc                                                                                                                                                    >  1:3630912/1‑150 (MQ=255)
    ggCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCa                                                                                                                                                   >  1:3758713/1‑150 (MQ=255)
    ggCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCa                                                                                                                                                   <  1:1662007/150‑1 (MQ=255)
     gCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCaa                                                                                                                                                  <  2:3204054/150‑1 (MQ=255)
      cACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCaaa                                                                                                                                                 >  2:2761241/1‑150 (MQ=255)
      cACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCaaa                                                                                                                                                 >  2:472378/1‑150 (MQ=255)
        cGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGc                                                                                                                                               <  1:2020945/150‑1 (MQ=255)
              cTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGAt                                                                                                                                         >  2:3673151/1‑150 (MQ=255)
                 cGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGa                                                                                                                                      >  1:3610314/1‑150 (MQ=255)
                    gAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAAt                                                                                                                                   >  2:1031575/1‑150 (MQ=255)
                      ataatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATag                                                                                                                                 >  2:2043147/1‑150 (MQ=255)
                       taatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATaga                                                                                                                                <  1:2132614/150‑1 (MQ=255)
                        aatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATagag                                                                                                                               >  1:1895838/1‑150 (MQ=255)
                        aatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATagag                                                                                                                               <  2:2641190/150‑1 (MQ=255)
                          tGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCa                                                                                                                             <  1:892406/150‑1 (MQ=255)
                            cccTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACt                                                                                                                           <  1:1154191/150‑1 (MQ=255)
                             ccTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTg                                                                                                                          >  2:3921929/1‑150 (MQ=255)
                             ccTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCAATGATTTGCGGGATACGCCCCTCATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTg                                                                                                                          >  2:111108/1‑150 (MQ=255)
                              cTTCGCATCGGTTTCTGTATCCGGGTTCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGCATAAGACACTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTgg                                                                                                                         >  1:4436469/1‑150 (MQ=255)
                                   cATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAg                                                                                                                    <  1:3999295/150‑1 (MQ=255)
                                   cATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAg                                                                                                                    >  2:1177988/1‑150 (MQ=255)
                                     tCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGc                                                                                                                  >  1:1019202/1‑150 (MQ=255)
                                     tCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGc                                                                                                                  >  1:3291779/1‑150 (MQ=255)
                                       ggTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTc                                                                                                                <  1:4196712/150‑1 (MQ=255)
                                        gTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCg                                                                                                               <  1:229008/150‑1 (MQ=255)
                                         tttCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGc                                                                                                              >  1:4041516/1‑150 (MQ=255)
                                              gtgtCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATg                                                                                                         >  2:3031488/4‑150 (MQ=255)
                                              gTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATg                                                                                                         <  1:1068296/150‑1 (MQ=255)
                                                aTCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGcc                                                                                                       <  1:2939262/150‑1 (MQ=255)
                                                     ggATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGgtgt                                                                                                  <  2:880961/150‑1 (MQ=255)
                                                        tCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATc                                                                                               >  1:3351672/1‑150 (MQ=255)
                                                         caccacCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATcc                                                                                              >  2:604601/1‑150 (MQ=255)
                                                           ccaccaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTg                                                                                            >  1:2912079/1‑150 (MQ=255)
                                                            caccaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTgg                                                                                           >  2:3575749/1‑150 (MQ=255)
                                                              ccaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCg                                                                                         <  2:652236/150‑1 (MQ=255)
                                                               caGCATCGGTTTAATTTTCCCTACAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCgg                                                                                        <  2:1481565/150‑1 (MQ=255)
                                                                aGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCggg                                                                                       >  2:4373420/1‑150 (MQ=255)
                                                                 gCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGTTGTATCCTGGCgggg                                                                                      <  1:3686095/150‑1 (MQ=255)
                                                                    tCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTcc                                                                                   <  2:537709/150‑1 (MQ=255)
                                                                     cGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCa                                                                                  >  2:2083347/1‑150 (MQ=255)
                                                                      ggTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAg                                                                                 >  1:2666573/1‑150 (MQ=255)
                                                                       gTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGa                                                                                >  1:1497814/1‑150 (MQ=255)
                                                                        tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               <  1:1312913/150‑1 (MQ=255)
                                                                        tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               >  1:853775/1‑150 (MQ=255)
                                                                        tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               >  1:853885/1‑150 (MQ=255)
                                                                        tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               >  2:3843415/1‑150 (MQ=255)
                                                                          tAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacat                                                                             <  2:134763/150‑1 (MQ=255)
                                                                          tAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacat                                                                             <  2:4251304/150‑1 (MQ=255)
                                                                            aTTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatac                                                                           >  2:4529705/1‑150 (MQ=255)
                                                                              tttCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatacat                                                                         <  1:2393127/150‑1 (MQ=255)
                                                                              tttCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatacat                                                                         >  2:2530412/1‑150 (MQ=255)
                                                                                 cccTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTg                                                                      <  2:3767563/150‑1 (MQ=255)
                                                                                 cccTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTg                                                                      <  1:102684/150‑1 (MQ=255)
                                                                                  ccTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGATATACATCTGa                                                                     >  1:2051398/1‑150 (MQ=255)
                                                                                    ttCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACg                                                                   >  1:2804930/1‑150 (MQ=255)
                                                                                     tCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGt                                                                  <  2:2232240/150‑1 (MQ=255)
                                                                                       agcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTc                                                                <  1:249945/150‑1 (MQ=255)
                                                                                       agcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTc                                                                >  1:1804863/1‑150 (MQ=255)
                                                                                       agcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTc                                                                <  2:1510168/150‑1 (MQ=255)
                                                                                        gcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCa                                                               <  1:2878156/150‑1 (MQ=255)
                                                                                           agcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGAt                                                            >  1:1568849/1‑150 (MQ=255)
                                                                                           agcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGAt                                                            <  2:3438556/150‑1 (MQ=255)
                                                                                             caGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTg                                                          <  2:781576/150‑1 (MQ=255)
                                                                                               ggTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCCTGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCa                                                        <  2:2477866/150‑1 (MQ=255)
                                                                                                gTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCaa                                                       >  1:4297826/1‑150 (MQ=255)
                                                                                                   aTCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGc                                                    <  1:2440548/150‑1 (MQ=255)
                                                                                                     ccATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGt                                                  >  1:1632227/1‑150 (MQ=255)
                                                                                                       aTGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTg                                                >  2:4116644/1‑150 (MQ=255)
                                                                                                        tGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTgg                                               <  1:2220067/150‑1 (MQ=255)
                                                                                                         gATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGa                                              >  1:3069404/1‑150 (MQ=255)
                                                                                                            ttGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTg                                           >  2:656671/1‑150 (MQ=255)
                                                                                                             tGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTgg                                          >  2:2474500/1‑150 (MQ=255)
                                                                                                             tGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTgg                                          <  1:2884842/150‑1 (MQ=255)
                                                                                                                gggATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTaa                                       <  2:3296692/150‑1 (MQ=255)
                                                                                                                gggATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTaa                                       >  1:4098641/1‑150 (MQ=255)
                                                                                                                   aTACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGtt                                    <  1:4531454/150‑1 (MQ=255)
                                                                                                                    tACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTa                                   >  1:1557408/1‑150 (MQ=255)
                                                                                                                    tACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTa                                   <  2:2860527/150‑1 (MQ=255)
                                                                                                                     aCGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTAt                                  <  1:1837160/150‑1 (MQ=255)
                                                                                                                        ccccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGc                               <  1:3472407/150‑1 (MQ=255)
                                                                                                                         cccTGATCGATAGCGGAACGTCCGTTATCACCTAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCTGTGTATCCTGGCGGGGTCCAGACATTCATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCg                              <  2:3122990/150‑1 (MQ=255)
                                                                                                                         cccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCg                              >  2:1835495/1‑150 (MQ=255)
                                                                                                                         cccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCg                              >  1:1888173/1‑150 (MQ=255)
                                                                                                                             gATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCa                          >  1:2817705/1‑150 (MQ=255)
                                                                                                                             gATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCa                          <  1:1342895/150‑1 (MQ=255)
                                                                                                                              aTCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCaa                         <  2:2912079/150‑1 (MQ=255)
                                                                                                                                    aGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCAcc                   >  1:1578214/1‑150 (MQ=255)
                                                                                                                                    aGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCAcc                   <  1:1959747/150‑1 (MQ=255)
                                                                                                                                    aGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCAcc                   <  1:964188/150‑1 (MQ=255)
                                                                                                                                       ggAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATg                >  1:1249078/1‑150 (MQ=255)
                                                                                                                                       ggAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATg                <  2:2090876/150‑1 (MQ=255)
                                                                                                                                        gAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATgc               >  1:2854104/1‑150 (MQ=255)
                                                                                                                                              ccGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACa         <  2:1116171/150‑1 (MQ=255)
                                                                                                                                                  tataACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAc     <  1:604601/150‑1 (MQ=255)
                                                                                                                                                    tCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAcgc   >  1:2768797/1‑150 (MQ=255)
                                                                                                                                                     cACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAcgcg  <  2:1895838/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCG  >  CP009273/3893236‑3893534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑