Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,895,052 G→T 5.2% R177R (CGG→AGG)  bglH ← carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,895,0520GT 5.2% 1038.6 / 18.9 273R177R (CGG→AGG) bglHcarbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base G (141/117);  new base T (8/7);  total (149/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

AGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGT  >  CP009273/3894903‑3895201
                                                                                                                                                     |                                                                                                                                                     
  ccTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGtt                                                                                                                                                     <  2:655979/150‑1 (MQ=255)
   ctctGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGttt                                                                                                                                                    <  2:1465827/150‑1 (MQ=255)
   ctctGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGttt                                                                                                                                                    >  2:3203655/1‑150 (MQ=255)
   ctctGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGttt                                                                                                                                                    >  2:4337270/1‑150 (MQ=255)
    tctGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTg                                                                                                                                                   <  1:327633/150‑1 (MQ=255)
      tGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGcc                                                                                                                                                 >  1:1905140/1‑150 (MQ=255)
       ggCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCg                                                                                                                                                >  2:2805728/1‑150 (MQ=255)
        gcgcAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGt                                                                                                                                               <  1:2048747/150‑1 (MQ=255)
        gcgcAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGt                                                                                                                                               <  1:2537352/150‑1 (MQ=255)
             aaGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCGGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCTTTTGCCGTTTTCg                                                                                                                                          >  1:4442788/1‑150 (MQ=255)
               gggCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGtt                                                                                                                                        <  1:3450279/150‑1 (MQ=255)
                ggCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTg                                                                                                                                       >  1:555446/1‑150 (MQ=255)
                 gCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGt                                                                                                                                      >  1:3629096/1‑150 (MQ=255)
                   aGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAg                                                                                                                                    >  1:2427859/1‑150 (MQ=255)
                   aGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAg                                                                                                                                    >  1:2427638/1‑150 (MQ=255)
                      aaaCCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGaca                                                                                                                                 <  1:4322285/150‑1 (MQ=255)
                        aCCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACg                                                                                                                               <  2:10232/150‑1 (MQ=255)
                          cTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAACCCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgtt                                                                                                                             <  1:745574/150‑1 (MQ=255)
                             tggtggTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgttgtt                                                                                                                          >  2:1513030/1‑150 (MQ=255)
                             tggtggTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCAACCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgttgtt                                                                                                                          <  2:4203998/150‑1 (MQ=255)
                              ggtggtAACGTACATATCGGAGAACTGCATATAGGTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGttt                                                                                                                         <  2:3739934/150‑1 (MQ=255)
                                    aaCGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTg                                                                                                                   >  1:633512/1‑150 (MQ=255)
                                    aaCGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTg                                                                                                                   <  1:1214072/150‑1 (MQ=255)
                                       gTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCaa                                                                                                                >  2:1190926/1‑150 (MQ=255)
                                       gTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCaa                                                                                                                >  2:943345/1‑150 (MQ=255)
                                       gTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCaa                                                                                                                >  1:3380068/1‑150 (MQ=255)
                                          cATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATc                                                                                                             >  2:3456362/1‑150 (MQ=255)
                                          cATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATc                                                                                                             >  1:3532006/1‑150 (MQ=255)
                                            tatCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCaa                                                                                                           <  2:3966254/150‑1 (MQ=255)
                                            tatCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCaa                                                                                                           >  1:918326/1‑150 (MQ=255)
                                                ggAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCa                                                                                                       >  1:3193648/1‑150 (MQ=255)
                                                 gagaACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAg                                                                                                      <  1:1193639/150‑1 (MQ=255)
                                                  agaACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGc                                                                                                     <  1:3918164/150‑1 (MQ=255)
                                                     aCTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCTTTTGCCGTTTACGTTGTAGACACGTTGATTTAACTGCAAATCAAACCAGACgg                                                                                                  >  2:2377529/1‑150 (MQ=255)
                                                        gCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGt                                                                                               >  2:2872934/1‑150 (MQ=255)
                                                         cATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCTTTTGCCGTTTTCGTTGTAGACACGTTGTTTTCACTGCAAAGCAAACCAGCCGGAGTa                                                                                              >  2:241897/1‑150 (MQ=255)
                                                             taGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCg                                                                                          <  2:491408/150‑1 (MQ=255)
                                                              aGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGt                                                                                         >  1:3150903/1‑150 (MQ=255)
                                                               gTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGtt                                                                                        <  1:3494359/150‑1 (MQ=255)
                                                                  ctcGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTAcc                                                                                     >  2:2627691/1‑150 (MQ=255)
                                                                  ctcGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTAcc                                                                                     >  2:2627705/1‑150 (MQ=255)
                                                                  ctcGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTAcc                                                                                     >  1:1101561/1‑150 (MQ=255)
                                                                    cGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCaa                                                                                   <  2:4003362/150‑1 (MQ=255)
                                                                    cGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCaa                                                                                   >  2:3892750/1‑150 (MQ=255)
                                                                    cGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCaa                                                                                   >  2:4045916/1‑150 (MQ=255)
                                                                        aCCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCg                                                                               >  1:3602534/1‑150 (MQ=255)
                                                                          cGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGATACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTAGTATACACGTAGTTTTAACTGCAAAACAAACCAGCCGGAGTATACGTTACCAAAGAGGc                                                                             >  1:2959361/1‑150 (MQ=255)
                                                                            gCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGccc                                                                           <  1:2621082/150‑1 (MQ=255)
                                                                             cGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCTAAGCGGCCCa                                                                          <  2:4520852/150‑1 (MQ=255)
                                                                               ttATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCaga                                                                        <  1:2631412/150‑1 (MQ=255)
                                                                               ttATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCaga                                                                        <  1:1667952/150‑1 (MQ=255)
                                                                                 aTCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAgaga                                                                      >  2:454842/1‑150 (MQ=255)
                                                                                  tCGCCAAACCAGCCGGTAGAGTTCTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGaa                                                                     <  2:2475253/150‑1 (MQ=255)
                                                                                   cGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAAc                                                                    >  2:1905563/1‑150 (MQ=255)
                                                                                       aaaCCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAAt                                                                <  1:106063/150‑1 (MQ=255)
                                                                                            aGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGccc                                                           >  2:1315641/1‑150 (MQ=255)
                                                                                              ccGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCAt                                                         >  2:4435973/1‑150 (MQ=255)
                                                                                                 gTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGAt                                                      >  1:1609045/1‑150 (MQ=255)
                                                                                                 gTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGAt                                                      <  2:361410/150‑1 (MQ=255)
                                                                                                   agagTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCCACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGAttt                                                    >  1:4475531/1‑150 (MQ=255)
                                                                                                   agagTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGAttt                                                    >  1:4475417/1‑150 (MQ=255)
                                                                                                     agTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAg                                                  <  2:2345331/150‑1 (MQ=255)
                                                                                                        aCTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTg                                               <  1:2559882/150‑1 (MQ=255)
                                                                                                         ctgctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGa                                              >  2:760379/1‑150 (MQ=255)
                                                                                                            ctgACCAACGTTACTATCCATCATCACTACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAAcc                                           <  2:2915634/150‑1 (MQ=255)
                                                                                                            ctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAAcc                                           <  2:404819/150‑1 (MQ=255)
                                                                                                               aCCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATg                                        <  2:1716155/150‑1 (MQ=255)
                                                                                                                ccAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCAGGATTTAGGTGAACCATga                                       >  2:3561608/1‑150 (MQ=255)
                                                                                                                 cAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag                                      <  1:3749914/150‑1 (MQ=255)
                                                                                                                  aaCGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgaga                                     >  1:2524015/1‑150 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCTTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  >  1:2714026/1‑150 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  >  1:324866/1‑150 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  >  1:337253/1‑150 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  <  2:1887891/150‑1 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  <  2:1886369/150‑1 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  >  2:1825192/1‑150 (MQ=255)
                                                                                                                     gTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGc                                  >  2:2524009/1‑150 (MQ=255)
                                                                                                                         ccatccatCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTc                              <  1:1513030/150‑1 (MQ=255)
                                                                                                                          catccatcATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTcc                             <  2:3749834/150‑1 (MQ=255)
                                                                                                                           atccatcATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc                            >  1:3901043/1‑150 (MQ=255)
                                                                                                                           atccatcATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc                            <  1:3988599/150‑1 (MQ=255)
                                                                                                                                tcatcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAc                       <  2:279081/150‑1 (MQ=255)
                                                                                                                                 cctcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc                      <  2:2116680/148‑1 (MQ=255)
                                                                                                                                 catcaCAACGTCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc                      <  2:3320213/150‑1 (MQ=255)
                                                                                                                                 catcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc                      >  2:1279310/1‑150 (MQ=255)
                                                                                                                                  atcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCAGGATTTAGGTGACACATGAGAGGCGGTCCCCCAACCa                     >  2:2858602/1‑150 (MQ=255)
                                                                                                                                   tcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAg                    <  2:3150903/150‑1 (MQ=255)
                                                                                                                                      caACGGCATCAACCCTTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAAc                 >  2:2532553/1‑150 (MQ=255)
                                                                                                                                       aaCGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACg                >  1:472701/1‑150 (MQ=255)
                                                                                                                                       aaCGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACg                >  1:471695/1‑150 (MQ=255)
                                                                                                                                          ggCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTa             >  1:1612933/1‑150 (MQ=255)
                                                                                                                                           gCATCATCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCTGAGTATTCGTTACCAAAGCGGCCCAGAGATCCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGACCGGTaa            <  2:633463/150‑1 (MQ=255)
                                                                                                                                           gCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTaa            <  2:22454/150‑1 (MQ=255)
                                                                                                                                                aaCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGcc       >  2:3695494/1‑150 (MQ=255)
                                                                                                                                                aaCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGcc       >  2:3503193/1‑150 (MQ=255)
                                                                                                                                                 aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCg      <  1:2917052/150‑1 (MQ=255)
                                                                                                                                                    cGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTg   <  1:2338156/150‑1 (MQ=255)
                                                                                                                                                     gTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGt  >  2:3836522/1‑150 (MQ=255)
                                                                                                                                                     gTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGt  >  2:3817654/1‑150 (MQ=255)
                                                                                                                                                     gTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGt  >  2:1558797/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGT  >  CP009273/3894903‑3895201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑