Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,906,261 C→A 6.7% E256D (GAG→GAT)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,906,2610CA 6.7% 1111.7 / 35.4 297E256D (GAG→GAT) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (115/161);  new base A (8/12);  total (123/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 312 total aligned reads displayed.

TTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAG  >  CP009273/3906112‑3906410
                                                                                                                                                     |                                                                                                                                                     
ttATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGAtc                                                                                                                                                       <  1:1171560/150‑1 (MQ=255)
ttATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGAtc                                                                                                                                                       >  2:2554684/1‑150 (MQ=255)
  ataAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGAtctc                                                                                                                                                     <  2:3926993/150‑1 (MQ=255)
   taAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATctct                                                                                                                                                    >  1:2337020/1‑150 (MQ=255)
     agaagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCttt                                                                                                                                                  <  2:2583185/150‑1 (MQ=255)
         gTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGc                                                                                                                                              <  1:3838088/150‑1 (MQ=255)
              acaGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTa                                                                                                                                         >  2:2981832/1‑150 (MQ=255)
               caGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAg                                                                                                                                        >  1:2247398/1‑150 (MQ=255)
               caGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAg                                                                                                                                        <  2:3030693/150‑1 (MQ=255)
                aGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGt                                                                                                                                       >  1:3149285/1‑150 (MQ=255)
                   cGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGAc                                                                                                                                    <  2:3003014/150‑1 (MQ=255)
                    gAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACg                                                                                                                                   >  2:4258130/1‑150 (MQ=255)
                      ggATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCTTTCGGCTGTTCGTAGATGTCTTTCTGCATGTAGTGACGGt                                                                                                                                 <  1:4441621/150‑1 (MQ=255)
                       gATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTa                                                                                                                                <  1:1695581/150‑1 (MQ=255)
                        aTCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTaa                                                                                                                               >  1:554244/1‑150 (MQ=255)
                         tCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTaaa                                                                                                                              >  1:1150370/1‑150 (MQ=255)
                          cTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTTTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAAt                                                                                                                             <  2:4100444/150‑1 (MQ=255)
                          cTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAAt                                                                                                                             <  1:4195810/150‑1 (MQ=255)
                          cTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAAt                                                                                                                             <  2:3806913/150‑1 (MQ=255)
                          cTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAAt                                                                                                                             <  2:3806992/150‑1 (MQ=255)
                            gAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGc                                                                                                                           >  1:940199/1‑150 (MQ=255)
                              atatGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCt                                                                                                                         >  2:875197/1‑150 (MQ=255)
                                atGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCttt                                                                                                                       <  1:761535/150‑1 (MQ=255)
                                     cAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGc                                                                                                                  >  2:4157347/1‑150 (MQ=255)
                                      aaCCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGcc                                                                                                                 >  1:2656877/1‑150 (MQ=255)
                                      aaCCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGcc                                                                                                                 >  1:2484930/1‑150 (MQ=255)
                                      aaCCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGcc                                                                                                                 <  1:1926592/150‑1 (MQ=255)
                                      aaCCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGcc                                                                                                                 >  2:362906/1‑150 (MQ=255)
                                      aaCCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGcc                                                                                                                 >  1:3212518/1‑150 (MQ=255)
                                        ccTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGAAGGTAAATGCCTTTATCGCCcg                                                                                                               <  2:1921595/150‑1 (MQ=255)
                                           tCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGt                                                                                                            <  1:1032731/150‑1 (MQ=255)
                                              aCAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCat                                                                                                         >  1:1514619/1‑150 (MQ=255)
                                               cagcagTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCata                                                                                                        <  2:1782340/150‑1 (MQ=255)
                                                   agTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGc                                                                                                    >  2:4473239/1‑150 (MQ=255)
                                                   agTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGc                                                                                                    >  2:115308/1‑150 (MQ=255)
                                                   agTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGc                                                                                                    >  1:2998299/1‑150 (MQ=255)
                                                    gTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGGTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCa                                                                                                   <  1:2637870/150‑1 (MQ=255)
                                                        gtcgGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGAtt                                                                                               >  1:810435/1‑150 (MQ=255)
                                                        gtcgGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGAtt                                                                                               >  2:3191640/1‑150 (MQ=255)
                                                        gtcgGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGAtt                                                                                               <  1:1067088/150‑1 (MQ=255)
                                                        gtcgGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGAtt                                                                                               <  2:1500745/150‑1 (MQ=255)
                                                           ggCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGa                                                                                            >  1:3446928/1‑150 (MQ=255)
                                                             cGTTCGGTCCCTGCTCGCTTAAATCTACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTTGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGAtt                                                                                          <  1:1908004/150‑1 (MQ=255)
                                                             cGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGAtt                                                                                          <  2:2358465/150‑1 (MQ=255)
                                                              gTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTc                                                                                         <  1:3075665/150‑1 (MQ=255)
                                                              gTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTc                                                                                         <  1:1769588/150‑1 (MQ=255)
                                                               ttCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCg                                                                                        <  2:2737486/150‑1 (MQ=255)
                                                                tCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGa                                                                                       >  1:1013225/1‑150 (MQ=255)
                                                                 cGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGat                                                                                      >  2:3799671/1‑150 (MQ=255)
                                                                 cGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGat                                                                                      <  2:3336694/150‑1 (MQ=255)
                                                                 cGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGat                                                                                      <  2:3115675/150‑1 (MQ=255)
                                                                 cGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGat                                                                                      <  2:3115385/150‑1 (MQ=255)
                                                                  ggTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGata                                                                                     <  1:509279/150‑1 (MQ=255)
                                                                     cccAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATcc                                                                                  <  1:3979243/150‑1 (MQ=255)
                                                                       cAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTg                                                                                >  1:3280665/1‑150 (MQ=255)
                                                                         gCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGAc                                                                              <  1:2130291/150‑1 (MQ=255)
                                                                           tCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGt                                                                            <  1:308525/150‑1 (MQ=255)
                                                                           tCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGGAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCATTATCGCCCGCGTCATCTTGCAGATTGGATTCGATATCCTGACGt                                                                            >  1:2901632/1‑150 (MQ=255)
                                                                              cTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGtttt                                                                         <  2:522497/150‑1 (MQ=255)
                                                                               ttAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTa                                                                        <  1:1829212/150‑1 (MQ=255)
                                                                                   aTCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTc                                                                    >  2:1129554/1‑150 (MQ=255)
                                                                                           gACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAg                                                            >  1:3572655/1‑150 (MQ=255)
                                                                                           gACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAg                                                            <  1:994692/150‑1 (MQ=255)
                                                                                             ccGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGtt                                                          >  1:2778318/1‑150 (MQ=255)
                                                                                             ccGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGtt                                                          >  2:4368170/1‑150 (MQ=255)
                                                                                                  gCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATc                                                     <  1:1357750/150‑1 (MQ=255)
                                                                                                   cTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCg                                                    <  2:2803399/150‑1 (MQ=255)
                                                                                                     gATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGaa                                                  >  1:2748980/1‑150 (MQ=255)
                                                                                                      aTGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAg                                                 <  1:4388769/150‑1 (MQ=255)
                                                                                                      aTGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAg                                                 <  1:363576/150‑1 (MQ=255)
                                                                                                        gCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGAt                                               <  2:2680399/150‑1 (MQ=255)
                                                                                                        gCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGAt                                               <  2:2681552/150‑1 (MQ=255)
                                                                                                         cGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATg                                              <  2:4332419/150‑1 (MQ=255)
                                                                                                          gTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGt                                             <  2:3748876/150‑1 (MQ=255)
                                                                                                            ccGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGttt                                           >  2:815070/1‑150 (MQ=255)
                                                                                                            ccGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGttt                                           <  2:1443409/150‑1 (MQ=255)
                                                                                                               gTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTAcc                                        <  2:538420/150‑1 (MQ=255)
                                                                                                                   gggTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAAc                                    <  2:115564/150‑1 (MQ=255)
                                                                                                                    ggTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACg                                   <  1:1280612/150‑1 (MQ=255)
                                                                                                                     gtgtTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACgg                                  <  1:968931/150‑1 (MQ=255)
                                                                                                                     gtgtTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACgg                                  >  1:910299/1‑150 (MQ=255)
                                                                                                                              aTCGCGTTCGGCTGTTCGTAGATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGAtt                         <  1:1665341/150‑1 (MQ=255)
                                                                                                                               tCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGAttt                        <  2:2819022/150‑1 (MQ=255)
                                                                                                                                cgcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTc                       <  2:742188/150‑1 (MQ=255)
                                                                                                                                cgcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTc                       >  1:3922053/1‑150 (MQ=255)
                                                                                                                                    ttCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCa                   <  2:1300927/150‑1 (MQ=255)
                                                                                                                                     tCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCaa                  >  1:402081/1‑150 (MQ=255)
                                                                                                                                        gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAatat               <  2:3351506/150‑1 (MQ=255)
                                                                                                                                         cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATc              >  1:4182658/1‑150 (MQ=255)
                                                                                                                                         cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATc              <  2:2247398/150‑1 (MQ=255)
                                                                                                                                         cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATc              <  1:4258130/150‑1 (MQ=255)
                                                                                                                                           gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGc            >  1:3601942/1‑150 (MQ=255)
                                                                                                                                           gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGc            >  1:2735123/1‑150 (MQ=255)
                                                                                                                                            ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGGCATATTGCAGATTGGATTCGATATCCTGACGAGTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc           >  2:3326343/1‑150 (MQ=255)
                                                                                                                                             tCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGccc          <  1:217319/150‑1 (MQ=255)
                                                                                                                                              cGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCct         <  2:2315999/150‑1 (MQ=255)
                                                                                                                                               gTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCctc        >  2:3981085/1‑150 (MQ=255)
                                                                                                                                                tAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCctct       <  2:613689/150‑1 (MQ=255)
                                                                                                                                                   aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCa    <  1:333348/150‑1 (MQ=255)
                                                                                                                                                     ctctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAg  <  1:1450597/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAG  >  CP009273/3906112‑3906410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑