Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,926,774 G→T 5.1% D22Y (GAT→TAT)  rbsD → putative cytoplasmic sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,926,7740GT 5.1% 1015.2 / 20.6 272D22Y (GAT→TAT) rbsDputative cytoplasmic sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (151/107);  new base T (5/9);  total (156/116)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 278 total aligned reads displayed.

ACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAA  >  CP009273/3926625‑3926923
                                                                                                                                                     |                                                                                                                                                     
acaTTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCg                                                                                                                                                       >  1:4238542/1‑150 (MQ=255)
     tCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACg                                                                                                                                                  >  1:794150/1‑150 (MQ=255)
     tCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACg                                                                                                                                                  >  1:3075889/1‑150 (MQ=255)
     tCCGTAACGTCACGATGGTTTTCCCAACCCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACg                                                                                                                                                  >  1:794230/1‑150 (MQ=255)
       cGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCt                                                                                                                                                >  2:2575312/1‑150 (MQ=255)
       cGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCt                                                                                                                                                >  2:2319662/1‑150 (MQ=255)
       cGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCt                                                                                                                                                <  1:894142/150‑1 (MQ=255)
       cGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCt                                                                                                                                                <  1:742716/150‑1 (MQ=255)
         tAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCCGTCTTAATTCTTATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCtgg                                                                                                                                              >  1:759494/1‑150 (MQ=255)
          aaCGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACTTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCtggt                                                                                                                                             >  2:4367458/1‑150 (MQ=255)
          aaCGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCtggt                                                                                                                                             <  2:863928/150‑1 (MQ=255)
                 cGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGgtgtgt                                                                                                                                      >  2:4034849/1‑150 (MQ=255)
                  gATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTAATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGtgtgtg                                                                                                                                     >  1:1169270/1‑150 (MQ=255)
                     ggTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCTCTGATGGAGAAAAAAATGAAAAAAGGCACCGATCATAATAATGATATTTAATCGGTGATCTACCGACTGGGACATACCGAAACTCTGGTGGTTTGTGATg                                                                                                                                  >  1:4178224/1‑150 (MQ=255)
                      gTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGc                                                                                                                                 <  1:1529001/150‑1 (MQ=255)
                       ttttCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCt                                                                                                                                <  1:93488/150‑1 (MQ=255)
                        tttCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTg                                                                                                                               <  1:3771020/150‑1 (MQ=255)
                           cccAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGtt                                                                                                                            <  2:2102015/150‑1 (MQ=255)
                           cccAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGtt                                                                                                                            <  1:2810973/150‑1 (MQ=255)
                           cccAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGATGGtt                                                                                                                            >  2:3492949/1‑150 (MQ=255)
                                   agtcagGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATc                                                                                                                    >  1:4085075/1‑150 (MQ=255)
                                       agGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCa                                                                                                                <  2:1249407/150‑1 (MQ=255)
                                       agGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCa                                                                                                                <  1:253127/150‑1 (MQ=255)
                                            tAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGt                                                                                                           >  2:2175777/1‑150 (MQ=255)
                                            tAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGt                                                                                                           >  2:2432603/1‑150 (MQ=255)
                                                   tgGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAAcgc                                                                                                    >  2:2444875/1‑150 (MQ=255)
                                                      gTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTa                                                                                                 >  2:1766782/1‑150 (MQ=255)
                                                       tCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTAt                                                                                                <  2:3594757/150‑1 (MQ=255)
                                                         aGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATct                                                                                              <  1:1937850/150‑2 (MQ=255)
                                                         aGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATct                                                                                              <  1:4182513/150‑2 (MQ=255)
                                                            gAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCtata                                                                                           >  1:3264226/1‑150 (MQ=255)
                                                                cGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGc                                                                                       <  2:1151993/150‑1 (MQ=255)
                                                                cGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGc                                                                                       <  2:2228160/150‑1 (MQ=255)
                                                                  tttCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCAt                                                                                     >  2:1386625/1‑150 (MQ=255)
                                                                       cTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAAcc                                                                                >  1:2253676/1‑150 (MQ=255)
                                                                         gATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCa                                                                              <  2:4238806/150‑1 (MQ=255)
                                                                         gATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCa                                                                              >  1:4301839/1‑150 (MQ=255)
                                                                          aTGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAg                                                                             >  1:2466505/1‑150 (MQ=255)
                                                                             gagaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgt                                                                          >  2:4488597/1‑150 (MQ=255)
                                                                              agaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgtg                                                                         <  1:2021073/150‑1 (MQ=255)
                                                                              agaAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGgtg                                                                         >  2:374694/1‑150 (MQ=255)
                                                                                aaaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTa                                                                       >  1:2491253/1‑150 (MQ=255)
                                                                                aaaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTa                                                                       >  1:3529614/1‑150 (MQ=255)
                                                                                 aaaaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTAc                                                                      >  1:2347847/1‑150 (MQ=255)
                                                                                   aaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACct                                                                    <  2:1441239/150‑1 (MQ=255)
                                                                                   aaaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACct                                                                    >  2:4141396/1‑150 (MQ=255)
                                                                                    aaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACctt                                                                   >  2:561655/1‑150 (MQ=255)
                                                                                    aaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACctt                                                                   >  2:673273/1‑150 (MQ=255)
                                                                                    aaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACctt                                                                   >  1:666364/1‑150 (MQ=255)
                                                                                    aaaTGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACctt                                                                   >  1:3321205/1‑150 (MQ=255)
                                                                                       tGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACcttctt                                                                <  1:1010218/150‑1 (MQ=255)
                                                                                       tGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACcttctt                                                                <  2:197916/150‑1 (MQ=255)
                                                                                         aaaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCtttt                                                              <  1:57002/150‑1 (MQ=255)
                                                                                          aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTa                                                             <  2:4460392/150‑1 (MQ=255)
                                                                                          aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTa                                                             >  2:69482/1‑150 (MQ=255)
                                                                                           aaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAt                                                            <  1:2396014/150‑1 (MQ=255)
                                                                                            aaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                                                           <  2:1120586/150‑1 (MQ=255)
                                                                                             aaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCGCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGc                                                          <  2:1065252/150‑1 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        <  2:794150/150‑1 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        >  1:1593397/1‑150 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        >  2:1602543/1‑150 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        <  2:3075889/150‑1 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        <  2:794271/150‑1 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        >  2:1837804/1‑150 (MQ=255)
                                                                                               ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                                                        >  2:4093913/1‑150 (MQ=255)
                                                                                                gCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAgg                                                       <  2:371930/150‑1 (MQ=255)
                                                                                                gCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAgg                                                       <  2:1922768/150‑1 (MQ=255)
                                                                                                 cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGt                                                      <  1:2096440/150‑1 (MQ=255)
                                                                                                 cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGt                                                      <  1:2096812/150‑1 (MQ=255)
                                                                                                 cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGt                                                      <  1:1050905/150‑1 (MQ=255)
                                                                                                   ccGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGc                                                    >  2:3192867/1‑150 (MQ=255)
                                                                                                    cGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCt                                                   <  2:338874/150‑1 (MQ=255)
                                                                                                     gTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTg                                                  >  2:4199321/1‑150 (MQ=255)
                                                                                                     gTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTg                                                  <  2:2786660/150‑1 (MQ=255)
                                                                                                     gTTCTTAATACTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTg                                                  >  1:4543869/1‑150 (MQ=255)
                                                                                                      ttCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTgg                                                 >  1:1320523/1‑150 (MQ=255)
                                                                                                      ttCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTgg                                                 >  1:4435145/1‑150 (MQ=255)
                                                                                                       tCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTggg                                                <  1:3438768/150‑1 (MQ=255)
                                                                                                              tCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCgtcgtc                                         <  2:1810576/150‑1 (MQ=255)
                                                                                                              tCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCgtcgtc                                         >  2:4134539/1‑150 (MQ=255)
                                                                                                               cTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTca                                        >  1:2724923/1‑150 (MQ=255)
                                                                                                               cTGAGATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTca                                        <  1:2702191/150‑1 (MQ=255)
                                                                                                                  atatTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaa                                     <  1:83679/150‑1 (MQ=255)
                                                                                                                  atatTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaa                                     >  1:3523101/1‑150 (MQ=255)
                                                                                                                      ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatga                                 >  1:2838190/1‑150 (MQ=255)
                                                                                                                      ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatga                                 >  1:551545/1‑150 (MQ=255)
                                                                                                                       tCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatgaa                                >  2:3314753/1‑150 (MQ=255)
                                                                                                                        cATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGGCGTCAAaaatgaaa                               >  2:3997197/1‑150 (MQ=255)
                                                                                                                         aTCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatgaaat                              <  1:3618822/150‑1 (MQ=255)
                                                                                                                           cGGTGATCTCCCGTCTGGGACATACCTATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGc                            >  1:182697/1‑150 (MQ=255)
                                                                                                                               gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGt                        >  1:1234461/1‑150 (MQ=255)
                                                                                                                                aTCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTc                       <  1:1681362/150‑1 (MQ=255)
                                                                                                                                 tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCg                      <  1:1602543/150‑1 (MQ=255)
                                                                                                                                 tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCg                      <  1:4093913/150‑1 (MQ=255)
                                                                                                                                    cccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAgg                   >  1:2065129/1‑150 (MQ=255)
                                                                                                                                     ccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAggc                  <  1:2771967/150‑1 (MQ=255)
                                                                                                                                          tGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCa             >  1:3934392/1‑150 (MQ=255)
                                                                                                                                            ggACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCAtt           <  1:1037353/150‑1 (MQ=255)
                                                                                                                                                aTACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATcg       <  2:3877055/150‑1 (MQ=255)
                                                                                                                                                     gATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCTATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGaa  >  1:421980/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATGCAGGTCGAGGCGGCCATTATCGCGGAA  >  CP009273/3926625‑3926923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑