Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,068,208 C→A 92.4% G60G (GGC→GGA)  yihW → DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,068,2080CA92.4% 811.0 / 52.3 274G60G (GGC→GGA) yihWDeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/4);  new base A (119/134);  total (136/138)
Fisher's exact test for biased strand distribution p-value = 2.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAG  >  CP009273/4068061‑4068355
                                                                                                                                                   |                                                                                                                                                   
gCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGgagc                                                                                                                                                   >  1:129325/1‑150 (MQ=255)
 cACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGagcg                                                                                                                                                  <  1:993186/150‑1 (MQ=255)
     aCCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTc                                                                                                                                              <  2:3631826/150‑1 (MQ=255)
     aCCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTc                                                                                                                                              <  2:2109105/150‑1 (MQ=255)
       cAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCgg                                                                                                                                            >  2:4455807/1‑150 (MQ=255)
       cAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCgg                                                                                                                                            >  2:3672081/1‑150 (MQ=255)
        aaCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCggg                                                                                                                                           >  1:1334386/1‑150 (MQ=255)
          ctcctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCt                                                                                                                                         >  2:1391214/1‑150 (MQ=255)
             ctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTcc                                                                                                                                      <  2:1046567/150‑1 (MQ=255)
              tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCa                                                                                                                                     <  2:3212123/150‑1 (MQ=255)
              tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCa                                                                                                                                     >  1:2392492/1‑150 (MQ=255)
                    tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcg                                                                                                                               >  2:4351867/1‑150 (MQ=255)
                    tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTACCAGcgtcg                                                                                                                               >  1:586860/1‑150 (MQ=255)
                      aTCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGtt                                                                                                                             >  1:4427769/1‑150 (MQ=255)
                       tCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTa                                                                                                                            >  2:1107185/1‑150 (MQ=255)
                         gCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAAt                                                                                                                          <  2:3981882/150‑1 (MQ=255)
                         gCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAAt                                                                                                                          <  2:2470615/150‑1 (MQ=255)
                          ccGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATa                                                                                                                         >  1:2368199/1‑150 (MQ=255)
                           cGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATAc                                                                                                                        <  1:2652903/150‑1 (MQ=255)
                             aGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACgg                                                                                                                      >  1:1090737/1‑150 (MQ=255)
                               cGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                                                    <  2:2401042/150‑1 (MQ=255)
                                  gggTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTc                                                                                                                 >  1:1027986/1‑150 (MQ=255)
                                     tataTGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGag                                                                                                              <  1:4111673/150‑1 (MQ=255)
                                         tGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagcagc                                                                                                          >  2:1375493/1‑150 (MQ=255)
                                         tGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagcagc                                                                                                          >  2:1376010/1‑150 (MQ=255)
                                          gAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCg                                                                                                         >  2:1047895/1‑150 (MQ=255)
                                           aaTATTGCTGTGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                        <  2:4079753/150‑1 (MQ=255)
                                           aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                        >  2:2603434/1‑150 (MQ=255)
                                            atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCGGCGTg                                                                                                       >  2:3587355/1‑150 (MQ=255)
                                            atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTg                                                                                                       >  1:1308138/1‑150 (MQ=255)
                                               ttGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAgg                                                                                                    <  2:1909561/150‑1 (MQ=255)
                                                 gatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGtt                                                                                                  <  2:2550611/150‑1 (MQ=255)
                                                  atgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGttt                                                                                                 >  2:2868276/1‑150 (MQ=255)
                                                   tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                                <  2:4268781/150‑1 (MQ=255)
                                                   tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                                <  1:3469591/150‑1 (MQ=255)
                                                    gaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCg                                                                                               >  1:4495307/1‑150 (MQ=255)
                                                    gaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCg                                                                                               <  1:1683693/150‑1 (MQ=255)
                                                     aGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGc                                                                                              >  1:2201607/1‑150 (MQ=255)
                                                         ggCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAAc                                                                                          >  1:77404/1‑150 (MQ=255)
                                                             aaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAg                                                                                      >  1:4156239/1‑150 (MQ=255)
                                                                 tgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaa                                                                                  <  1:692797/150‑1 (MQ=255)
                                                                   ctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaa                                                                                <  1:3017189/150‑1 (MQ=255)
                                                                   ctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaa                                                                                <  1:193665/150‑1 (MQ=255)
                                                                    tgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaa                                                                               >  1:3483734/1‑150 (MQ=255)
                                                                    tgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaa                                                                               >  1:1771297/1‑150 (MQ=255)
                                                                     ggATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaaa                                                                              <  1:798828/150‑1 (MQ=255)
                                                                       aTGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAg                                                                            >  1:2512923/1‑150 (MQ=255)
                                                                        tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                           >  2:4011762/1‑150 (MQ=255)
                                                                        tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                           >  2:4011983/1‑150 (MQ=255)
                                                                        tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                           <  2:1058903/150‑1 (MQ=255)
                                                                        tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                           >  2:1705135/1‑150 (MQ=255)
                                                                              cACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGACGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGc                                                                     >  2:3816525/1‑150 (MQ=255)
                                                                                cGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCg                                                                   <  1:131206/150‑1 (MQ=255)
                                                                                   aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                                >  1:1412153/1‑150 (MQ=255)
                                                                                   aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                                >  1:2245997/1‑150 (MQ=255)
                                                                                     aCGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCg                                                              >  2:2181407/1‑150 (MQ=255)
                                                                                      cGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAACAAAAAGCGATTGCCGAAGCgg                                                             >  2:2707912/1‑150 (MQ=255)
                                                                                        gTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTg                                                           <  1:3530399/150‑1 (MQ=255)
                                                                                        gTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTg                                                           <  2:4254212/150‑1 (MQ=255)
                                                                                           cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                        >  2:2272473/1‑150 (MQ=255)
                                                                                             ccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGa                                                      <  2:4376715/150‑1 (MQ=255)
                                                                                              cgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGAc                                                     >  1:3840230/1‑150 (MQ=255)
                                                                                                  atatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtata                                                 >  1:557727/1‑150 (MQ=255)
                                                                                                   tatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTatat                                                >  2:543988/1‑150 (MQ=255)
                                                                                                   tatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTatat                                                <  1:2875166/150‑1 (MQ=255)
                                                                                                       cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            <  1:2514301/150‑1 (MQ=255)
                                                                                                       cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            <  2:2137436/150‑1 (MQ=255)
                                                                                                          aaaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGAt                                         <  1:2868276/150‑1 (MQ=255)
                                                                                                            aTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATgg                                       >  1:464709/1‑150 (MQ=255)
                                                                                                              tAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGtt                                     <  2:2968195/150‑1 (MQ=255)
                                                                                                                aGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                                   <  2:4443002/150‑1 (MQ=255)
                                                                                                                aGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                                   <  2:4051609/150‑1 (MQ=255)
                                                                                                                aGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                                   >  1:880842/1‑150 (MQ=255)
                                                                                                                  cGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                 >  2:797895/1‑150 (MQ=255)
                                                                                                                  cGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                 >  2:798484/1‑150 (MQ=255)
                                                                                                                   gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                                <  1:2948847/150‑1 (MQ=255)
                                                                                                                     gCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAat                              <  2:1542305/150‑1 (MQ=255)
                                                                                                                      cAAGGCCTGATTACGCGCCATCCCGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                             <  1:801140/150‑1 (MQ=255)
                                                                                                                      cAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                             <  1:3549693/150‑1 (MQ=255)
                                                                                                                       aaGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAatat                            <  2:1433179/150‑1 (MQ=255)
                                                                                                                          gCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTa                         <  2:1274563/150‑1 (MQ=255)
                                                                                                                           ccTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTAt                        <  2:2417816/150‑1 (MQ=255)
                                                                                                                           ccTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTAGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTAt                        >  1:4087795/1‑150 (MQ=255)
                                                                                                                            cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                       <  2:281246/150‑1 (MQ=255)
                                                                                                                            cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                       >  2:2701770/1‑150 (MQ=255)
                                                                                                                             tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                      >  2:697824/1‑150 (MQ=255)
                                                                                                                              gATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCAc                     >  2:866010/1‑150 (MQ=255)
                                                                                                                                  aCGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCAtt                 >  1:3618825/1‑150 (MQ=255)
                                                                                                                                   cgcgCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTg                <  1:2944142/150‑1 (MQ=255)
                                                                                                                                    gcgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTgg               <  2:3714381/150‑1 (MQ=255)
                                                                                                                                    gcgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTgg               <  2:4117883/150‑1 (MQ=255)
                                                                                                                                     cgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGt              <  1:2074751/150‑1 (MQ=255)
                                                                                                                                     cgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGt              >  1:3403683/1‑150 (MQ=255)
                                                                                                                                       ccATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTAc            <  2:4156306/150‑1 (MQ=255)
                                                                                                                                        cATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACg           <  1:967299/150‑1 (MQ=255)
                                                                                                                                        cATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACg           <  1:4169781/150‑1 (MQ=255)
                                                                                                                                         aTCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGa          >  1:2741498/1‑150 (MQ=255)
                                                                                                                                         aTCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGa          >  1:478966/1‑150 (MQ=255)
                                                                                                                                           cACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACt        <  2:2201607/150‑1 (MQ=255)
                                                                                                                                             cGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGt      <  1:2274272/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAG  >  CP009273/4068061‑4068355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑