BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,211,576	G→T	93.0%	S280R (AGC→AGA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,211,576	0	G	T	93.0%	983.6 / 57.3	329	S280R (AGC→AGA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (7/16); new base T (139/166); total (146/183)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 330 total aligned reads displayed.

TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAT  >  CP009273/4211429‑4211722

  ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCa                                                                                                                                                <  1:3645280/150‑1 (MQ=255)

      cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAAt                                                                                                                                            <  1:3064815/150‑1 (MQ=255)

       aTCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAAtt                                                                                                                                           <  1:4411817/150‑1 (MQ=255)

         cAATAGCAGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCt                                                                                                                                         <  2:2854447/150‑1 (MQ=255)

           aTAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTa                                                                                                                                       >  1:3864864/1‑150 (MQ=255)

            tAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAt                                                                                                                                      >  1:4264374/1‑150 (MQ=255)

            tAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAt                                                                                                                                      <  2:2842875/150‑1 (MQ=255)

              gCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAttt                                                                                                                                    <  2:4319940/150‑1 (MQ=255)

              gCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAttt                                                                                                                                    >  2:3560960/1‑150 (MQ=255)

               cTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAtttt                                                                                                                                   >  2:4479201/1‑150 (MQ=255)

                tGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTa                                                                                                                                  >  1:161549/1‑150 (MQ=255)

                tGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTa                                                                                                                                  <  2:3367177/150‑1 (MQ=255)

                tGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTa                                                                                                                                  <  2:4370429/150‑1 (MQ=255)

                 gTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATAAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTAt                                                                                                                                 >  1:930343/1‑150 (MQ=255)

                  ttCTTTCGGAAGTTCTTGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATc                                                                                                                                <  2:3631853/150‑1 (MQ=255)

                    cTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgt                                                                                                                              <  2:2101183/150‑1 (MQ=255)

                     tttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCTTCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtt                                                                                                                             <  1:3026451/150‑1 (MQ=255)

                     tttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtt                                                                                                                             <  2:1004103/150‑1 (MQ=255)

                     tttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtt                                                                                                                             <  1:1254046/150‑1 (MQ=255)

                      ttCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgtta                                                                                                                            <  1:297541/150‑1 (MQ=255)

                       tCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGTATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttag                                                                                                                           >  2:3262633/1‑150 (MQ=255)

                            aGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACa                                                                                                                      <  1:1966491/150‑1 (MQ=255)

                              tGCTGTAATTATTGCAACAGATTGATTAATAACATGATCATATGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCATAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACAtt                                                                                                                    >  2:4404082/1‑150 (MQ=255)

                              tGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACAtt                                                                                                                    <  1:2563282/150‑1 (MQ=255)

                                 tGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAgg                                                                                                                 <  1:2384828/150‑1 (MQ=255)

                                    aaTTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATc                                                                                                              >  2:3173770/1‑150 (MQ=255)

                                    aaTTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATc                                                                                                              >  2:1211933/1‑150 (MQ=255)

                                       tattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATa                                                                                                           <  1:1295345/150‑1 (MQ=255)

                                       tattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATa                                                                                                           >  1:4004740/1‑150 (MQ=255)

                                       tataGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATa                                                                                                           <  2:1279658/150‑1 (MQ=255)

                                         ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaaa                                                                                                         <  2:2824507/150‑1 (MQ=255)

                                         ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaaa                                                                                                         <  1:3049301/150‑1 (MQ=255)

                                           gCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAAcc                                                                                                       >  2:3308682/1‑150 (MQ=255)

                                            cAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCg                                                                                                      <  1:4176095/150‑1 (MQ=255)

                                            cAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCg                                                                                                      <  1:3750619/150‑1 (MQ=255)

                                             aaCAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATAGTTAGTTACATTAGGATCATAAACCGa                                                                                                     >  2:4441424/1‑150 (MQ=255)

                                                aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                  >  2:1941470/1‑150 (MQ=255)

                                                aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTCGCCCCCCCCCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                  <  1:362565/150‑1 (MQ=255)

                                                aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAAAAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                  >  2:1054582/1‑150 (MQ=255)

                                                    tgattAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACg                                                                                              <  1:3167132/150‑1 (MQ=255)

                                                           taaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATggg                                                                                       <  1:2485130/150‑1 (MQ=255)

                                                                 gATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATc                                                                                 <  2:3264439/150‑1 (MQ=255)

                                                                 gATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATc                                                                                 <  2:280651/150‑1 (MQ=255)

                                                                  aTCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                <  1:2323859/150‑1 (MQ=255)

                                                                  aTCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                <  1:2324475/150‑1 (MQ=255)

                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTc                                                                             >  1:2453864/1‑150 (MQ=255)

                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTc                                                                             <  2:3147041/150‑1 (MQ=255)

                                                                      tttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCa                                                                            >  1:1285873/1‑150 (MQ=255)

                                                                      tttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCa                                                                            >  2:3036974/1‑150 (MQ=255)

                                                                      tttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCa                                                                            <  1:611129/150‑1 (MQ=255)

                                                                        tGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGt                                                                          >  1:1085534/1‑150 (MQ=255)

                                                                          aCCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtg                                                                        >  1:2647521/1‑150 (MQ=255)

                                                                             aTTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTgtgt                                                                     <  2:1887255/150‑1 (MQ=255)

                                                                             aTTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTgtgt                                                                     <  2:4139626/150‑1 (MQ=255)

                                                                              tttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGtt                                                                    <  1:1138990/150‑1 (MQ=255)

                                                                                    gaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTAt                                                              >  2:1672746/1‑150 (MQ=255)

                                                                                      ggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTc                                                            >  1:2958872/1‑150 (MQ=255)

                                                                                         tataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTa                                                         <  1:2036049/150‑1 (MQ=255)

                                                                                          ataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAg                                                        >  2:888637/1‑150 (MQ=255)

                                                                                          ataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAg                                                        <  2:4397375/150‑1 (MQ=255)

                                                                                             aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                     >  1:4376703/1‑150 (MQ=255)

                                                                                             aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                     <  1:2497918/150‑1 (MQ=255)

                                                                                             aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                     <  2:2176697/150‑1 (MQ=255)

                                                                                              tCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCgg                                                    >  1:1543474/1‑150 (MQ=255)

                                                                                              tCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCgg                                                    >  1:1542097/1‑150 (MQ=255)

                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                  >  1:3711004/1‑150 (MQ=255)

                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                  <  2:26901/150‑1 (MQ=255)

                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                  <  1:1034101/150‑1 (MQ=255)

                                                                                                 aCATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACCTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAcc                                                 <  1:4475798/150‑1 (MQ=255)

                                                                                                  cATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCg                                                >  1:1406653/1‑150 (MQ=255)

                                                                                                      cATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCaa                                            >  2:1231733/1‑150 (MQ=255)

                                                                                                       aTAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCaaa                                           >  2:1758190/1‑150 (MQ=255)

                                                                                                            aTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATg                                      <  2:3486400/150‑1 (MQ=255)

                                                                                                             tCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATgg                                     <  1:2665318/150‑1 (MQ=255)

                                                                                                              ccATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGt                                    <  1:240378/150‑1 (MQ=255)

                                                                                                                aTCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTa                                  >  1:1710438/1‑150 (MQ=255)

                                                                                                                 tCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTAt                                 <  1:1782925/150‑1 (MQ=255)

                                                                                                                   agagAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcc                               >  1:499140/1‑150 (MQ=255)

                                                                                                                      gaATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                            <  1:1822605/150‑1 (MQ=255)

                                                                                                                       aaTAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                           >  2:939246/1‑150 (MQ=255)

                                                                                                                        aTAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAg                          >  1:3564609/1‑150 (MQ=255)

                                                                                                                          aGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTa                        >  2:3924940/1‑150 (MQ=255)

                                                                                                                          aGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCATCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTa                        >  1:142944/1‑150 (MQ=255)

                                                                                                                            tgtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTCat                      >  2:723873/1‑150 (MQ=255)

                                                                                                                             gtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAata                     >  1:81670/1‑150 (MQ=255)

                                                                                                                             gtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAata                     >  1:81718/1‑150 (MQ=255)

                                                                                                                              tttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAatat                    >  1:4144029/1‑150 (MQ=255)

                                                                                                                               ttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATg                   >  1:2595726/1‑150 (MQ=255)

                                                                                                                                tAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATgg                  <  1:3365738/150‑1 (MQ=255)

                                                                                                                                tAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACAGTCAAATAATGGTTATCCACCAGTAATATgg                  >  1:1382304/1‑150 (MQ=255)

                                                                                                                                 aaTATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGc                 <  1:2722870/150‑1 (MQ=255)

                                                                                                                                 aaTATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGc                 >  2:500618/1‑150 (MQ=255)

                                                                                                                                   tatTCACTTTGATTTCTATAATAATTCTTATTTTATCGTTAGATACATTAGGATCATAATCCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGTTAATACACCACTAatatcgatg               >  2:2593014/1‑145 (MQ=255)

                                                                                                                                         ctTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATaa         <  1:3971323/150‑1 (MQ=255)

                                                                                                                                          tttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac        <  1:4066268/150‑1 (MQ=255)

                                                                                                                                          tttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac        >  1:4429903/1‑150 (MQ=255)

                                                                                                                                          tttGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac        <  1:4245610/150‑1 (MQ=255)

                                                                                                                                          tttGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac        <  1:4245692/150‑1 (MQ=255)

                                                                                                                                           ttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAaca       <  1:3309551/150‑1 (MQ=255)

                                                                                                                                                ttCTCTCATAATTCTTATTTTATCGTTAGATACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACAGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAt  >  2:4116254/1‑150 (MQ=255)

TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAT  >  CP009273/4211429‑4211722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`