BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,250,664	A→C	9.8%	A46A (GCA→GCC)	yjbM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,250,664	0	A	C	9.8%	795.9 / 80.0	287	A46A (GCA→GCC)	yjbM	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (140/119); new base C (16/12); total (156/131)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 293 total aligned reads displayed.

AATGATCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTA  >  CP009273/4250515‑4250812

aatGATCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCa                                                                                                                                                      >  1:2523594/1‑150 (MQ=255)

   gATCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATg                                                                                                                                                   <  1:1542811/150‑1 (MQ=255)

    aTCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGc                                                                                                                                                  <  2:790336/150‑1 (MQ=255)

    aTCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGc                                                                                                                                                  >  2:2372115/1‑150 (MQ=255)

     tctcAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGatgccatgcc                                                                                                                                                 >  1:3931037/1‑150 (MQ=255)

      ctcAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGccc                                                                                                                                                <  2:1397545/150‑1 (MQ=255)

        cAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCAt                                                                                                                                              <  2:3031468/150‑1 (MQ=255)

          aaaTGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTg                                                                                                                                            <  2:449270/150‑1 (MQ=255)

          aaaTGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTg                                                                                                                                            <  1:4294154/150‑1 (MQ=255)

            aTGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGaa                                                                                                                                          <  1:3245052/150‑1 (MQ=255)

                 ggTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATg                                                                                                                                     >  1:1484580/1‑150 (MQ=255)

                   tCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTa                                                                                                                                   <  1:4202352/150‑1 (MQ=255)

                     aaTAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACt                                                                                                                                 <  2:2158451/150‑1 (MQ=255)

                      aTAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACtt                                                                                                                                >  1:2086564/1‑150 (MQ=255)

                      aTAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACtt                                                                                                                                >  1:461293/1‑150 (MQ=255)

                       tAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACttt                                                                                                                               >  2:3458867/1‑150 (MQ=255)

                        aaGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACtttt                                                                                                                              <  2:1714608/150‑1 (MQ=255)

                            atatTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAATATGCAATGCCCATTGAACAATGTACTTTTGATg                                                                                                                          >  2:325738/1‑150 (MQ=255)

                            atatTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATg                                                                                                                          >  1:1039918/1‑150 (MQ=255)

                            atatTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATg                                                                                                                          >  2:3627098/1‑150 (MQ=255)

                            atatTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTAATTTTGATg                                                                                                                          >  2:1358743/1‑150 (MQ=255)

                             tatTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGt                                                                                                                         <  2:1845582/150‑1 (MQ=255)

                               ttGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAg                                                                                                                       <  2:2645108/150‑1 (MQ=255)

                               ttGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAg                                                                                                                       <  2:1027507/150‑1 (MQ=255)

                                tGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGa                                                                                                                      >  1:853537/1‑150 (MQ=255)

                                 gACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGaa                                                                                                                     <  2:13395/150‑1 (MQ=255)

                                       tGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAg                                                                                                               <  1:1695153/150‑1 (MQ=255)

                                       tGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAg                                                                                                               >  1:2022648/1‑150 (MQ=255)

                                              atgaGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGAcc                                                                                                        <  2:2858314/150‑1 (MQ=255)

                                                   gATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTccc                                                                                                   <  2:3541877/150‑1 (MQ=255)

                                                   gATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTccc                                                                                                   <  2:1269496/150‑1 (MQ=255)

                                                       tAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCa                                                                                               >  2:513634/1‑150 (MQ=255)

                                                         aaCGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACg                                                                                             >  1:3746698/1‑150 (MQ=255)

                                                            gATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGtt                                                                                          >  2:2460057/1‑150 (MQ=255)

                                                              tCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCa                                                                                        <  1:3620826/150‑1 (MQ=255)

                                                                gTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGc                                                                                      >  2:1147251/1‑150 (MQ=255)

                                                                 tGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTGGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCt                                                                                     >  1:2860717/1‑150 (MQ=255)

                                                                 tGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCt                                                                                     >  1:487618/1‑150 (MQ=255)

                                                                  gACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTa                                                                                    >  1:2335065/1‑150 (MQ=255)

                                                                     tttATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTAttt                                                                                 <  2:482622/150‑1 (MQ=255)

                                                                      ttATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTAtttt                                                                                >  2:3800055/1‑150 (MQ=255)

                                                                      ttATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGAACTTCCCTCCACGGTTCAGCTAtttt                                                                                >  2:3363155/1‑150 (MQ=255)

                                                                        aTTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTAt                                                                              >  2:3750606/1‑150 (MQ=255)

                                                                          tGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGa                                                                            >  2:333956/1‑150 (MQ=255)

                                                                          tGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGa                                                                            >  2:1922622/1‑150 (MQ=255)

                                                                             aTCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGa                                                                         >  1:1550692/1‑150 (MQ=255)

                                                                             aTCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAAATAAGAATGACATTCCCTCCACGGTTCAGCTATTTTATGAGGa                                                                         >  1:1551344/1‑150 (MQ=255)

                                                                             aTCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGa                                                                         >  2:2406389/1‑150 (MQ=255)

                                                                             aTCAGTTGATGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGa                                                                         >  1:1830068/1‑150 (MQ=255)

                                                                               cAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAAt                                                                       >  2:3854902/1‑150 (MQ=255)

                                                                                   tgttgACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTaa                                                                   >  2:1812539/1‑150 (MQ=255)

                                                                                      tgACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGa                                                                >  2:2979078/1‑150 (MQ=255)

                                                                                       gACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGAt                                                               <  2:1176904/150‑1 (MQ=255)

                                                                                              cTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAAc                                                        <  2:3931037/150‑1 (MQ=255)

                                                                                              cTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAAc                                                        <  2:1670527/150‑1 (MQ=255)

                                                                                               tATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAAcc                                                       >  1:3572062/1‑150 (MQ=255)

                                                                                                aTTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCt                                                      >  2:4011135/1‑150 (MQ=255)

                                                                                                 tttttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCtt                                                     <  1:583071/150‑1 (MQ=255)

                                                                                                 tttttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCtt                                                     >  1:4397100/1‑150 (MQ=255)

                                                                                                 tttttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCtt                                                     <  2:3782827/150‑1 (MQ=255)

                                                                                                 tttttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCtt                                                     <  1:3186342/150‑1 (MQ=255)

                                                                                                   tttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTa                                                   <  1:2283024/150‑1 (MQ=255)

                                                                                                   tttCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTa                                                   <  1:3218219/150‑1 (MQ=255)

                                                                                                     tCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGc                                                 >  1:4141331/1‑150 (MQ=255)

                                                                                                       aTTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGa                                               >  2:4128800/1‑150 (MQ=255)

                                                                                                        tttCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCCTTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGaa                                              >  2:1451195/1‑150 (MQ=255)

                                                                                                         ttCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAat                                             <  1:2886632/150‑1 (MQ=255)

                                                                                                            gcgATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAAtata                                          <  2:4446331/150‑1 (MQ=255)

                                                                                                             cgATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATAc                                         >  1:115869/1‑150 (MQ=255)

                                                                                                             cgATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATAc                                         >  2:4272355/1‑150 (MQ=255)

                                                                                                               aTTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAt                                       <  1:246878/150‑1 (MQ=255)

                                                                                                               aTTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAt                                       <  1:2973755/150‑1 (MQ=255)

                                                                                                               aTTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAt                                       <  1:2548678/150‑1 (MQ=255)

                                                                                                                ttAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAtt                                      <  2:2313196/150‑1 (MQ=255)

                                                                                                                 tAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAttt                                     <  2:694743/150‑1 (MQ=255)

                                                                                                                 tAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAttt                                     >  2:2305276/1‑150 (MQ=255)

                                                                                                                  aaTAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAtttt                                    <  1:3567849/150‑1 (MQ=255)

                                                                                                                  aaTAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAtttt                                    <  2:461294/150‑1 (MQ=255)

                                                                                                                     aGTATATGTAATCCTGGGGATAATAAAGATGCCATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCaa                                 >  1:2779032/1‑150 (MQ=255)

                                                                                                                      gTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAg                                >  1:2073440/1‑150 (MQ=255)

                                                                                                                        atatGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCa                              <  2:2606187/150‑1 (MQ=255)

                                                                                                                         tctGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCaa                             <  1:671438/148‑1 (MQ=255)

                                                                                                                          atGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAAt                            <  1:3214906/150‑1 (MQ=255)

                                                                                                                           tGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATa                           >  2:2532589/1‑150 (MQ=255)

                                                                                                                           tGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATa                           >  2:1016607/1‑150 (MQ=255)

                                                                                                                            gTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAg                          >  1:1578259/1‑150 (MQ=255)

                                                                                                                            gTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAg                          >  1:3839836/1‑150 (MQ=255)

                                                                                                                             tAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACAt                                       <  2:4177812/136‑1 (MQ=255)

                                                                                                                                  cTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTg                    >  1:2050044/1‑150 (MQ=255)

                                                                                                                                   tGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTgg                   >  2:144188/1‑150 (MQ=255)

                                                                                                                                    ggggATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGt                  >  1:4014821/1‑150 (MQ=255)

                                                                                                                                      ggATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTTATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGttt                <  1:3458945/150‑1 (MQ=255)

                                                                                                                                      ggATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGttt                >  1:3979215/1‑150 (MQ=255)

                                                                                                                                      ggATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGttt                <  1:3458867/150‑1 (MQ=255)

                                                                                                                                       gATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGtttt               <  1:1271970/150‑1 (MQ=255)

                                                                                                                                         taataaAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGtttttt             <  1:378727/150‑1 (MQ=255)

                                                                                                                                           ataaAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTaa           >  1:1108435/1‑150 (MQ=255)

                                                                                                                                                gATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGttt      <  2:3188993/150‑1 (MQ=255)

                                                                                                                                                    cAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTa  <  2:29073/150‑1 (MQ=255)

                                                                                                                                                    cAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTa  >  1:4171719/1‑150 (MQ=255)

AATGATCTCAAAATGTGGGTCAATAAGTATATTGACGATTGCACTGATGAGGATTTAAACGATCGTGACTTTATTGCATCAGTTGTTGACCGGGCTATTTTTCATTTCGCGATTAATAGTATATGTAATCCTGGGGATAATAAAGATGCAATGCCCATTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTA  >  CP009273/4250515‑4250812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`