Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,142 C→A 93.3% intergenic (‑1/+199) yjcH ← / ← acs DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,1420CA93.3% 856.5 / 48.8 282intergenic (‑1/+199)yjcH/acsDUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (8/11);  new base A (145/118);  total (153/129)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 288 total aligned reads displayed.

CGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTC  >  CP009273/4274997‑4275288
                                                                                                                                                 |                                                                                                                                                  
cGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAg                                                                                                                                                >  2:3774572/1‑150 (MQ=255)
   gcgcGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAtt                                                                                                                                             <  1:121397/150‑1 (MQ=255)
   gcgcGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAtt                                                                                                                                             <  1:2980041/150‑1 (MQ=255)
         aGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCag                                                                                                                                       >  2:1624454/1‑150 (MQ=255)
            cGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAgg                                                                                                                                    >  2:687693/1‑150 (MQ=255)
            cGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAgg                                                                                                                                    <  1:2368007/150‑1 (MQ=255)
              aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                  <  2:3758956/150‑1 (MQ=255)
               tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTaa                                                                                                                                 >  2:3285521/1‑150 (MQ=255)
                   tAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCGGGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTa                                                                                                                             >  1:2069473/1‑150 (MQ=255)
                        tAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAt                                                                                                                        <  2:136168/150‑1 (MQ=255)
                          aaGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGACATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTg                                                                                                                      >  2:1215313/1‑150 (MQ=255)
                           aGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGt                                                                                                                     >  2:3242353/1‑150 (MQ=255)
                           aGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGt                                                                                                                     >  1:2477901/1‑150 (MQ=255)
                              cGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                  >  1:897232/1‑150 (MQ=255)
                              cGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                  <  2:4107132/150‑1 (MQ=255)
                               gATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTa                                                                                                                 <  2:846080/150‑1 (MQ=255)
                                 tataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAt                                                                                                               <  2:3986111/150‑1 (MQ=255)
                                   taAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                                                                                                             >  2:3257417/1‑150 (MQ=255)
                                    aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                            >  2:2793563/1‑150 (MQ=255)
                                     aaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                                                                                                           >  1:4402291/1‑150 (MQ=255)
                                     aaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                                                                                                           <  1:345793/150‑1 (MQ=255)
                                     aaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                                                                                                           >  1:2035837/1‑150 (MQ=255)
                                      aCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCg                                                                                                          >  1:3258939/1‑150 (MQ=255)
                                      aCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCg                                                                                                          <  2:92764/150‑1 (MQ=255)
                                      aCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCg                                                                                                          <  1:1975644/150‑1 (MQ=255)
                                        tGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        <  2:424063/150‑1 (MQ=255)
                                        tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        <  2:6046/150‑1 (MQ=255)
                                        tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        >  1:1912497/1‑150 (MQ=255)
                                        tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        <  2:2314086/150‑1 (MQ=255)
                                           cAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                                                                                                     <  2:697919/150‑1 (MQ=255)
                                           cAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                                                                                                     >  1:3061237/1‑150 (MQ=255)
                                              cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                  >  1:89725/1‑150 (MQ=255)
                                              cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                  >  1:89745/1‑150 (MQ=255)
                                              cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                  >  1:460852/1‑150 (MQ=255)
                                              cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                  >  1:1008979/1‑150 (MQ=255)
                                                 aataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                               <  2:2607706/150‑1 (MQ=255)
                                                    aatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgag                                                                                            <  1:679486/150‑1 (MQ=255)
                                                        gACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                        >  2:4254834/1‑150 (MQ=255)
                                                         aCAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGt                                                                                       >  1:4202966/1‑150 (MQ=255)
                                                             gATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc                                                                                   <  2:2477901/150‑1 (MQ=255)
                                                             gATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATGGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc                                                                                   <  2:3450674/150‑1 (MQ=255)
                                                              aTGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACg                                                                                  <  1:3201507/150‑1 (MQ=255)
                                                                ggtggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCt                                                                                >  1:3463997/1‑150 (MQ=255)
                                                                ggtggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCt                                                                                >  1:3463923/1‑150 (MQ=255)
                                                                   ggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCgg                                                                             <  2:1177590/150‑1 (MQ=255)
                                                                   ggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCgg                                                                             >  1:1466373/1‑150 (MQ=255)
                                                                    gCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCggg                                                                            >  1:503976/1‑150 (MQ=255)
                                                                     cAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGt                                                                           <  2:4537895/150‑1 (MQ=255)
                                                                      aaaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTa                                                                          <  2:1794011/150‑1 (MQ=255)
                                                                          cGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCATTATGGGAGAAGGTTAACGCTCGGGTAAccc                                                                      >  1:3674079/1‑150 (MQ=255)
                                                                           gTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCt                                                                     >  1:3207792/1‑150 (MQ=255)
                                                                           gTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCAATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCt                                                                     >  1:3700843/1‑150 (MQ=255)
                                                                            ttGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCtt                                                                    >  1:2843661/1‑150 (MQ=255)
                                                                               ccGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGcc                                                                 <  2:2848600/150‑1 (MQ=255)
                                                                                cGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCg                                                                <  1:2897162/150‑1 (MQ=255)
                                                                                cGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCg                                                                >  2:2202721/1‑150 (MQ=255)
                                                                                 gTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGa                                                               <  2:3704410/150‑1 (MQ=255)
                                                                                    tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATg                                                            <  1:1943753/150‑1 (MQ=255)
                                                                                     ttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGt                                                           >  1:2409824/1‑150 (MQ=255)
                                                                                      tCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTa                                                          <  1:2568595/150‑1 (MQ=255)
                                                                                      tCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTa                                                          >  1:3098372/1‑150 (MQ=255)
                                                                                      tCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTa                                                          <  2:429954/150‑1 (MQ=255)
                                                                                        gACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAgg                                                        >  1:1572633/1‑150 (MQ=255)
                                                                                        gACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAgg                                                        >  1:67885/1‑150 (MQ=255)
                                                                                         aCTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTACTGTAGGGATTGTTTTAATTCCCGCTCCCTGATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGc                                                       >  2:4367078/1‑150 (MQ=255)
                                                                                         aCTAACTCCCTGAAATGCGCATTGTCTTCGATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGc                                                       <  1:1336969/150‑1 (MQ=255)
                                                                                          cTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGcc                                                      >  2:2083062/1‑150 (MQ=255)
                                                                                              cTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGAt                                                  >  2:172220/1‑150 (MQ=255)
                                                                                                cccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATaa                                                <  1:2027990/150‑1 (MQ=255)
                                                                                                 ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAg                                               <  1:3437727/150‑1 (MQ=255)
                                                                                                 ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAg                                               >  1:380603/1‑150 (MQ=255)
                                                                                                   tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                             >  1:176819/1‑150 (MQ=255)
                                                                                                   tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                             <  2:207839/150‑1 (MQ=255)
                                                                                                    gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCg                                            <  2:3129926/150‑1 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                           >  1:84186/1‑150 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                           <  1:2375490/150‑1 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                           >  2:2002377/1‑150 (MQ=255)
                                                                                                     aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCGTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                           >  2:3592963/1‑150 (MQ=255)
                                                                                                          cgcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcg                                      >  2:1022573/1‑150 (MQ=255)
                                                                                                            cATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgcc                                    >  2:24836/1‑150 (MQ=255)
                                                                                                            cATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgcc                                    >  2:2329804/1‑150 (MQ=255)
                                                                                                             aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTGGGGGGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccg                                   >  2:363320/1‑150 (MQ=255)
                                                                                                              ttGTCTTCTATCCGCTGACAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCCTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccgc                                  >  1:1739840/1‑150 (MQ=255)
                                                                                                                   ttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                             <  2:3061237/150‑1 (MQ=255)
                                                                                                                   ttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                             <  2:1451301/150‑1 (MQ=255)
                                                                                                                   ttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                             <  2:2880132/150‑1 (MQ=255)
                                                                                                                     ctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                           >  2:1783662/1‑150 (MQ=255)
                                                                                                                                 aaaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTg               >  2:3830467/1‑150 (MQ=255)
                                                                                                                                  aaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGa              >  1:3917514/1‑150 (MQ=255)
                                                                                                                                  aaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGa              >  2:336487/1‑150 (MQ=255)
                                                                                                                                   aTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCAATGCCTGAt             <  2:154551/150‑1 (MQ=255)
                                                                                                                                   aTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGAt             <  2:183938/150‑1 (MQ=255)
                                                                                                                                   aTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGAt             >  2:2962795/1‑150 (MQ=255)
                                                                                                                                       tGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGa         >  1:1192169/1‑150 (MQ=255)
                                                                                                                                        gCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAGTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGAc        <  2:4181414/150‑1 (MQ=255)
                                                                                                                                         ccATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAGTCCCGCTCCCTTCTGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAATCAATGCCTGATGCGACg       <  2:4277853/150‑1 (MQ=255)
                                                                                                                                            ttCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg    <  1:2968968/150‑1 (MQ=255)
                                                                                                                                            ttCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg    >  1:4502660/1‑150 (MQ=255)
                                                                                                                                            gtcataaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg    <  1:3265028/149‑1 (MQ=255)
                                                                                                                                             tcataaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGt   <  1:765888/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTC  >  CP009273/4274997‑4275288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑