BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,276,940	G→T	49.5%	F120L (TTC→TTA)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,276,940	0	G	T	49.5%	124.7 / inf	295	F120L (TTC→TTA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (77/72); new base T (68/78); total (145/150)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

CTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTC  >  CP009273/4276792‑4277089

cTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGa                                                                                                                                                      >  1:1664300/1‑150 (MQ=255)

        gagaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAg                                                                                                                                              <  1:3276937/150‑1 (MQ=255)

         agaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTCTTGGCTAAGCGGCAGa                                                                                                                                             >  2:1727905/1‑150 (MQ=255)

          gaAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGAc                                                                                                                                            <  2:1163167/150‑1 (MQ=255)

           aaGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACg                                                                                                                                           >  2:3135806/1‑150 (MQ=255)

           aaGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACg                                                                                                                                           >  1:3844771/1‑150 (MQ=255)

            aGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGt                                                                                                                                          <  1:1303028/150‑1 (MQ=255)

            aGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGt                                                                                                                                          <  1:1538386/150‑1 (MQ=255)

            aGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGt                                                                                                                                          <  2:4250955/150‑1 (MQ=255)

             gccgccGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACggc                                                                                                                                         >  2:2856836/1‑148 (MQ=255)

               cgccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTcgc                                                                                                                                       >  1:230011/1‑150 (MQ=255)

                gccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTcgcg                                                                                                                                      <  1:3745983/150‑1 (MQ=255)

                 ccgAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCgg                                                                                                                                     >  1:1000837/1‑150 (MQ=255)

                     aaaTCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCa                                                                                                                                 >  1:1145841/1‑150 (MQ=255)

                        tCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGct                                                                                                                              <  2:542111/150‑1 (MQ=255)

                        tCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGACTTCCGGCACCATCGGCATCTAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGct                                                                                                                              >  1:3938337/1‑150 (MQ=255)

                        tCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGACTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGct                                                                                                                              >  1:1679875/1‑150 (MQ=255)

                         cACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGctc                                                                                                                             <  2:757652/150‑1 (MQ=255)

                         cACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGctc                                                                                                                             <  2:471154/150‑1 (MQ=255)

                          aCCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGctct                                                                                                                            <  1:2773980/150‑1 (MQ=255)

                           ccGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCtt                                                                                                                           <  2:1116232/150‑1 (MQ=255)

                             gAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTta                                                                                                                         <  1:629357/150‑1 (MQ=255)

                             gAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTta                                                                                                                         >  1:4541223/1‑150 (MQ=255)

                                   aCCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTg                                                                                                                   <  2:4385722/150‑1 (MQ=255)

                                     cgcgCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGat                                                                                                                 <  2:3275484/150‑1 (MQ=255)

                                       cgcCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCCGCTCGAGCAGGGTAGAGGCGACGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGatat                                                                                                               >  2:2607716/1‑150 (MQ=255)

                                        gcCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATg                                                                                                              <  2:2667713/150‑1 (MQ=255)

                                         ccAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGt                                                                                                             >  1:1561926/1‑150 (MQ=255)

                                          cAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGGGCAGCTCTTTATAGCTGATATGtt                                                                                                            <  1:4447499/150‑1 (MQ=255)

                                             tGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGc                                                                                                         >  2:586424/1‑150 (MQ=255)

                                                  gcgcAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctgg                                                                                                    <  2:4013069/150‑1 (MQ=255)

                                                      aGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTctggctgg                                                                                                >  1:4490950/1‑150 (MQ=255)

                                                        gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:1110676/150‑1 (MQ=255)

                                                        gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:381237/150‑1 (MQ=255)

                                                        gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:2085084/150‑1 (MQ=255)

                                                        gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:2085118/150‑1 (MQ=255)

                                                        gCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGcg                                                                                              <  2:2807628/150‑1 (MQ=255)

                                                             cATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCgtcgtc                                                                                         >  1:2555923/1‑150 (MQ=255)

                                                                cGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGcc                                                                                      >  2:2177788/1‑150 (MQ=255)

                                                                         ggCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAgat                                                                             <  1:2501280/150‑1 (MQ=255)

                                                                            ttCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAgatgat                                                                          <  2:2948547/150‑1 (MQ=255)

                                                                                ggCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCg                                                                      >  1:2217601/1‑150 (MQ=255)

                                                                                      aTCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGa                                                                <  1:1543232/150‑1 (MQ=255)

                                                                                       tCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGAt                                                               <  2:4375185/150‑1 (MQ=255)

                                                                                        cGGCATATAAATCTCCACCAAATCACATTTTTTTATGCCAAGATCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGATCTGGCTGGCGTCGTCGACTTCCCAGATGATGGCGGTACGATc                                                              <  2:3414739/150‑1 (MQ=255)

                                                                                        cGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATc                                                              <  1:886204/150‑1 (MQ=255)

                                                                                         ggCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCg                                                             <  2:123244/150‑1 (MQ=255)

                                                                                           cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           >  1:2646057/1‑150 (MQ=255)

                                                                                           cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           <  2:1005114/150‑1 (MQ=255)

                                                                                           cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           <  2:1293708/150‑1 (MQ=255)

                                                                                           cATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGcc                                                           >  1:2469939/1‑150 (MQ=255)

                                                                                            atatAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCg                                                          >  2:2423530/1‑150 (MQ=255)

                                                                                              ataAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtt                                                        <  2:1596905/150‑1 (MQ=255)

                                                                                              ataAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtt                                                        >  1:372349/1‑150 (MQ=255)

                                                                                               taAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                       >  1:831892/1‑150 (MQ=255)

                                                                                               taAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGttt                                                       <  1:4243036/150‑1 (MQ=255)

                                                                                                aaaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGtttt                                                      >  1:2451291/1‑150 (MQ=255)

                                                                                                 aaTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTc                                                     >  2:3558072/1‑150 (MQ=255)

                                                                                                  aTCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCt                                                    <  2:748275/150‑1 (MQ=255)

                                                                                                     gCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGc                                                 <  1:292465/150‑1 (MQ=255)

                                                                                                      ccaccaCATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCa                                                >  1:349482/1‑150 (MQ=255)

                                                                                                       caccacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAg                                               >  1:2251394/1‑150 (MQ=255)

                                                                                                       caccacATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAg                                               <  1:2407272/150‑1 (MQ=255)

                                                                                                          cacaTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATg                                            <  2:4160702/150‑1 (MQ=255)

                                                                                                          cacaTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATg                                            >  1:3534512/1‑150 (MQ=255)

                                                                                                           acaTCACCTTTTTTAATGCCCAGCTCGAGCTGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCAGTTTTCTTGCAGATgg                                           <  1:3681574/150‑1 (MQ=255)

                                                                                                             aTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                         <  1:4058389/150‑1 (MQ=255)

                                                                                                             aTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                         >  1:2671898/1‑150 (MQ=255)

                                                                                                             aTCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCg                                         >  1:3815925/1‑150 (MQ=255)

                                                                                                              tCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCgg                                        <  1:261437/150‑1 (MQ=255)

                                                                                                              tCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCgg                                        <  2:1212733/150‑1 (MQ=255)

                                                                                                                      tttAATGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCa                                <  1:1033868/150‑1 (MQ=255)

                                                                                                                        tAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGt                              <  2:3220765/150‑1 (MQ=255)

                                                                                                                         aaTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGtt                             >  2:291607/1‑150 (MQ=255)

                                                                                                                          aTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGttt                            >  2:4345271/1‑150 (MQ=255)

                                                                                                                          aTGCCCAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGttt                            >  2:4345201/1‑150 (MQ=255)

                                                                                                                           tGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTg                           <  2:898852/150‑1 (MQ=255)

                                                                                                                           tGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTg                           <  2:1944834/150‑1 (MQ=255)

                                                                                                                           tGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTg                           <  2:1944499/150‑1 (MQ=255)

                                                                                                                             cccAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTAGAGCTGATATGTGTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgcc                         <  2:213227/150‑1 (MQ=255)

                                                                                                                              ccAGCTCGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgccg                        >  1:4453555/1‑150 (MQ=255)

                                                                                                                                aGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTgccgcc                      <  2:1442652/150‑1 (MQ=255)

                                                                                                                                  cTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAg                    <  1:102643/150‑1 (MQ=255)

                                                                                                                                  cTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAg                    >  2:697456/1‑150 (MQ=255)

                                                                                                                                  cTCGAGCAGGGTATTGGCGAAGCGGCAGACCTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAg                    >  2:2979265/1‑150 (MQ=255)

                                                                                                                                    cGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAt                  >  1:2983421/1‑150 (MQ=255)

                                                                                                                                    cGAGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGAt                  >  1:1933949/1‑150 (MQ=255)

                                                                                                                                     gAGCAGGGTATTGGCTAAGCGGCAGACGTCGCTGTGCAGCTCTTTATAGCTGATATGTTTTCTCTGGCTGGCGTAGTAGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTAAAGGCAGTTTGCCGCCAGAtt                 >  2:3650818/1‑150 (MQ=255)

                                                                                                                                      aGCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTc                <  2:1841983/150‑1 (MQ=255)

                                                                                                                                       gCAGGGTATTGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCa               >  1:4362143/1‑150 (MQ=255)

                                                                                                                                        cAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAg              >  1:3420564/1‑150 (MQ=255)

                                                                                                                                         aGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGc             >  2:461180/1‑150 (MQ=255)

                                                                                                                                         aGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGc             >  2:3337724/1‑150 (MQ=255)

                                                                                                                                          gggTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCg            <  1:3361218/150‑1 (MQ=255)

                                                                                                                                          gggTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCg            <  1:2795788/150‑1 (MQ=255)

                                                                                                                                             tATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGAGATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGc         >  1:928200/1‑150 (MQ=255)

                                                                                                                                              aTTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGcc        <  2:2018584/150‑1 (MQ=255)

                                                                                                                                                tGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGt      >  1:1942354/1‑150 (MQ=255)

                                                                                                                                                tGGCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGt      >  2:2630571/1‑150 (MQ=255)

                                                                                                                                                 ggCTAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTc     >  2:2075604/1‑150 (MQ=255)

CTTCCGGCGAGAAGCCGCCGAAAATCACCGAATGCACCGCGCCAATGCGGGCGCAGGCCAGCATCGCAACCGCGGCTTCCGGCACCATCGGCATATAAATCGCCACCACATCACCTTTTTTAATGCCCAGCTCGAGCAGGGTATTGGCGAAGCGGCAGACGTCGCGGTGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTC  >  CP009273/4276792‑4277089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`