Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,295,218 C→A 12.2% M399I (ATG→ATT)  yjcS ← metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,295,2180CA12.2% 993.6 / 74.8 295M399I (ATG→ATT) yjcSmetallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (127/132);  new base A (16/20);  total (143/152)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 295 total aligned reads displayed.

TCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATAAACAG  >  CP009273/4295070‑4295364
                                                                                                                                                    |                                                                                                                                                  
 ctcCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACCCCGCGTGGGCGTTGTGGCTGACAGAACCGTCATAGCCGCGGCTGGCCCAGTTATTGGCATGTGCAGGCGGCAGCTTAATCAt                                                                                                                                                  <  1:4441145/150‑1 (MQ=255)
        tGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCg                                                                                                                                           >  1:1745011/1‑150 (MQ=255)
        tGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCg                                                                                                                                           <  2:4100267/150‑1 (MQ=255)
         gACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGa                                                                                                                                          >  1:2603252/1‑150 (MQ=255)
           ccATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGAtt                                                                                                                                        >  2:2226725/1‑150 (MQ=255)
             aTACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttc                                                                                                                                      >  1:2139863/1‑150 (MQ=255)
              tACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttca                                                                                                                                     <  2:3841221/150‑1 (MQ=255)
               aCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttcat                                                                                                                                    <  1:4239394/150‑1 (MQ=255)
                  gATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATttcattca                                                                                                                                 <  1:2910606/150‑1 (MQ=255)
                        ggTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGgtgt                                                                                                                           <  1:652205/150‑1 (MQ=255)
                         gTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTa                                                                                                                          >  2:4211952/1‑150 (MQ=255)
                         gTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTa                                                                                                                          <  1:3451583/150‑1 (MQ=255)
                         gTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTa                                                                                                                          >  2:1960217/1‑150 (MQ=255)
                              ccGGGTTACCGTCGTAATAGCCAAGATAGATGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCt                                                                                                                     <  1:1762026/150‑1 (MQ=255)
                              ccGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCt                                                                                                                     <  2:2070004/150‑1 (MQ=255)
                               cGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTg                                                                                                                    <  1:2528845/150‑1 (MQ=255)
                                gggTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTgg                                                                                                                   <  2:3065818/150‑1 (MQ=255)
                                gggTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTgg                                                                                                                   <  2:3619402/150‑1 (MQ=255)
                                gggTTACCGTCGTAAGAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTgg                                                                                                                   <  1:3845263/150‑1 (MQ=255)
                                   ttACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTc                                                                                                                >  2:1688301/1‑150 (MQ=255)
                                       cgtcgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCa                                                                                                            <  1:1099794/150‑1 (MQ=255)
                                       cgtcgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCa                                                                                                            <  1:2766678/150‑1 (MQ=255)
                                       cgtcgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCa                                                                                                            >  2:1048078/1‑150 (MQ=255)
                                       cgtcgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCa                                                                                                            <  2:821997/150‑1 (MQ=255)
                                          cgtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGt                                                                                                         <  1:2310379/150‑1 (MQ=255)
                                           gtAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTg                                                                                                        >  1:1361404/1‑150 (MQ=255)
                                              aTAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAg                                                                                                     >  2:1585895/1‑150 (MQ=255)
                                               tAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAgg                                                                                                    <  2:528311/150‑1 (MQ=255)
                                                aGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAggg                                                                                                   >  1:2861771/1‑150 (MQ=255)
                                                aGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAggg                                                                                                   >  1:1932016/1‑150 (MQ=255)
                                                  ccAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTc                                                                                                 >  1:611065/1‑150 (MQ=255)
                                                  ccAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTc                                                                                                 >  2:376872/1‑150 (MQ=255)
                                                    aaGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTg                                                                                               >  1:573512/1‑150 (MQ=255)
                                                        tAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCg                                                                                           <  2:4220576/150‑1 (MQ=255)
                                                         aGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGt                                                                                          >  1:1848242/1‑150 (MQ=255)
                                                         aGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGt                                                                                          >  2:2046919/1‑150 (MQ=255)
                                                          gAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTg                                                                                         >  1:2981968/1‑150 (MQ=255)
                                                          gAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTg                                                                                         <  2:1656138/150‑1 (MQ=255)
                                                          gAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTg                                                                                         <  2:2881888/150‑1 (MQ=255)
                                                            aGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGaa                                                                                       >  2:3369247/1‑150 (MQ=255)
                                                             gTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAAt                                                                                      <  1:2946061/150‑1 (MQ=255)
                                                               tataCACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGt                                                                                    >  2:254984/1‑150 (MQ=255)
                                                               tataCACCGCGCGGGCGTTGGGGCTGACAGAACCGTAAGAGCCGCGGCGGGCCCAGGTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGt                                                                                    <  2:271317/150‑1 (MQ=255)
                                                                ataCACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTa                                                                                   >  2:1766381/1‑150 (MQ=255)
                                                                 taCACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTAc                                                                                  <  1:1526765/150‑1 (MQ=255)
                                                                   cacCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACtt                                                                                >  1:3059039/1‑150 (MQ=255)
                                                                   cacCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACtt                                                                                <  1:1012767/150‑1 (MQ=255)
                                                                    acCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTg                                                                               <  2:2164211/150‑1 (MQ=255)
                                                                      cgcgcgGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGAt                                                                             <  1:2069412/150‑1 (MQ=255)
                                                                      cgcgcgGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGAt                                                                             >  1:2869826/1‑150 (MQ=255)
                                                                          cgGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTa                                                                         >  1:4331491/1‑150 (MQ=255)
                                                                          cgGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTa                                                                         <  2:1292447/150‑1 (MQ=255)
                                                                          cgGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTa                                                                         <  2:2814681/150‑1 (MQ=255)
                                                                           gggCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTAt                                                                        <  2:931896/150‑1 (MQ=255)
                                                                           gggCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTAt                                                                        >  2:1019618/1‑150 (MQ=255)
                                                                           gggCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTAt                                                                        >  1:1166733/1‑150 (MQ=255)
                                                                            ggCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATc                                                                       <  1:2549764/150‑1 (MQ=255)
                                                                             gCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATcg                                                                      <  2:1483977/150‑1 (MQ=255)
                                                                               gTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCAGTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATcgcg                                                                    >  2:3485307/1‑150 (MQ=255)
                                                                                 tgtgGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGt                                                                  <  2:153961/150‑1 (MQ=255)
                                                                                  gtgGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTa                                                                 >  1:3286247/1‑150 (MQ=255)
                                                                                    ggCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTAtt                                                               >  2:1637871/1‑150 (MQ=255)
                                                                                     gCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTAttt                                                              <  2:2925899/150‑1 (MQ=255)
                                                                                      cTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTa                                                             >  1:3556925/1‑150 (MQ=255)
                                                                                      cTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTa                                                             >  1:3556996/1‑150 (MQ=255)
                                                                                       tGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTAc                                                            <  2:3637407/150‑1 (MQ=255)
                                                                                        gACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTAcc                                                           >  2:3710187/1‑150 (MQ=255)
                                                                                        gACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCAGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTAcc                                                           <  2:469243/150‑1 (MQ=255)
                                                                                          cAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCaa                                                         <  1:2226725/150‑1 (MQ=255)
                                                                                          cAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGGAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCaa                                                         <  2:3440788/150‑1 (MQ=255)
                                                                                           aGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAat                                                        >  1:3666686/1‑150 (MQ=255)
                                                                                              aCCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAAtata                                                     <  1:3126459/150‑1 (MQ=255)
                                                                                               ccGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATaa                                                    <  1:826464/150‑1 (MQ=255)
                                                                                                 gTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATc                                                  >  1:2384190/1‑150 (MQ=255)
                                                                                                   aaTAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCAt                                                <  1:127738/150‑1 (MQ=255)
                                                                                                     tAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTg                                              >  1:1599298/1‑150 (MQ=255)
                                                                                                      aGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGa                                             >  2:476575/1‑150 (MQ=255)
                                                                                                       gCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGat                                            <  2:3234990/150‑1 (MQ=255)
                                                                                                       gCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGat                                            >  2:1826437/1‑150 (MQ=255)
                                                                                                        ccGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGata                                           <  2:1361417/150‑1 (MQ=255)
                                                                                                         cgcgGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGatat                                          >  1:1669295/1‑150 (MQ=255)
                                                                                                         cgcgGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGatat                                          >  1:3203712/1‑150 (MQ=255)
                                                                                                             gCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCtt                                      >  2:3926028/1‑150 (MQ=255)
                                                                                                                   ccAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGcc                                >  2:4117737/1‑150 (MQ=255)
                                                                                                                   ccAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGCTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGcc                                >  1:4128827/1‑150 (MQ=255)
                                                                                                                      gTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCa                             <  2:3826902/150‑1 (MQ=255)
                                                                                                                        tATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGa                           >  1:890431/1‑150 (MQ=255)
                                                                                                                         aTTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGAc                          <  1:3366503/150‑1 (MQ=255)
                                                                                                                         aTTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGGGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGAc                          >  2:4400641/1‑150 (MQ=255)
                                                                                                                          ttGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGTCCCAGAcc                         <  1:1963535/150‑1 (MQ=255)
                                                                                                                          ttGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGTCCCAGAcc                         <  1:4210980/150‑1 (MQ=255)
                                                                                                                          ttGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGAcc                         <  2:676886/150‑1 (MQ=255)
                                                                                                                           tGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCg                        <  1:4097545/150‑1 (MQ=255)
                                                                                                                             gCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGc                      >  1:1860000/1‑150 (MQ=255)
                                                                                                                                aGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAg                   >  2:1203228/1‑150 (MQ=255)
                                                                                                                                  tGCAGGCGGCAGCTTAATAATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGgt                 >  2:2372679/1‑150 (MQ=255)
                                                                                                                                            aGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATa       >  1:2186373/1‑150 (MQ=255)
                                                                                                                                             gCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATaa      <  2:498940/150‑1 (MQ=255)
                                                                                                                                               ttAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATAAAc    <  1:3999490/150‑1 (MQ=255)
                                                                                                                                                tAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATAAACa   >  1:3722366/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGCAGCTTAATCATGTCGCCGATTTCATTCATGGTGTAGCCCTGGTTCGCCAGGTGCAGGGTCTGGTCGTGAATGTACTTGATGGTATCGCGGTATTTACCAATATAATCATTGATATGCTTATTGCCCCAGACCGGCCAGGTGTGCGGCATAAACAG  >  CP009273/4295070‑4295364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑