Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,376,178 C→A 75.2% E1004* (GAA→TAA)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,376,1780CA75.2% 484.0 / 259.3 311E1004* (GAA→TAA) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (38/39);  new base A (123/111);  total (161/150)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 315 total aligned reads displayed.

TACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCGC  >  CP009273/4376031‑4376324
                                                                                                                                                   |                                                                                                                                                  
 aCGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCt                                                                                                                                                 <  2:11147/150‑1 (MQ=255)
  cGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTa                                                                                                                                                <  1:798435/150‑1 (MQ=255)
  cGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa                                                                                                                                                <  2:861686/150‑1 (MQ=255)
  cGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa                                                                                                                                                >  1:3752880/1‑150 (MQ=255)
    gTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTAtt                                                                                                                                              >  2:12079/1‑150 (MQ=255)
      gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTgg                                                                                                                                            <  2:2030188/150‑1 (MQ=255)
      gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTgg                                                                                                                                            <  2:243644/150‑1 (MQ=255)
        cccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCa                                                                                                                                          >  1:2818155/1‑150 (MQ=255)
        cccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCa                                                                                                                                          >  1:687034/1‑150 (MQ=255)
        cccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCa                                                                                                                                          >  1:2969743/1‑150 (MQ=255)
         ccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTCTTGGCAt                                                                                                                                         >  1:3307227/1‑150 (MQ=255)
          cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATc                                                                                                                                        <  2:741722/150‑1 (MQ=255)
          cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATc                                                                                                                                        <  1:4126124/150‑1 (MQ=255)
          cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATc                                                                                                                                        <  1:1876231/150‑1 (MQ=255)
           aTCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCg                                                                                                                                       >  1:913906/1‑150 (MQ=255)
            tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCgg                                                                                                                                      <  2:1682758/150‑1 (MQ=255)
            tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCgg                                                                                                                                      <  2:2848753/150‑1 (MQ=255)
                   ggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGc                                                                                                                               <  2:1035420/150‑1 (MQ=255)
                   ggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGc                                                                                                                               <  2:3372390/150‑1 (MQ=255)
                    gcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGcc                                                                                                                              <  2:4010215/150‑1 (MQ=255)
                     cgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCg                                                                                                                             >  2:1794391/1‑150 (MQ=255)
                     cgTAAAAACGCAGCTCGAAAATCTGAATCCCCTGGTGCAGATCCACCCGGACGACTTCCGGAGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCTTCGGCAGGGGCCg                                                                                                                             >  2:2321018/1‑150 (MQ=255)
                      gTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                            >  1:3189511/1‑150 (MQ=255)
                        aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTa                                                                                                                          >  1:289970/1‑150 (MQ=255)
                        aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTa                                                                                                                          <  1:1223193/150‑1 (MQ=255)
                        aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTa                                                                                                                          >  2:2566898/1‑150 (MQ=255)
                         aaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                         >  1:2535186/1‑150 (MQ=255)
                           tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCg                                                                                                                       <  2:2835991/150‑1 (MQ=255)
                               cAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCaa                                                                                                                   >  1:1997174/1‑150 (MQ=255)
                               cAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCaa                                                                                                                   >  1:1997425/1‑150 (MQ=255)
                                aGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAac                                                                                                                  >  1:3954072/1‑150 (MQ=255)
                                aGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGAGCCGGTATCGTCAac                                                                                                                  >  2:3027252/1‑150 (MQ=255)
                                        aaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcg                                                                                                          >  2:4441644/1‑150 (MQ=255)
                                        aaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcg                                                                                                          >  2:2295177/1‑150 (MQ=255)
                                        aaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcg                                                                                                          >  2:3304090/1‑150 (MQ=255)
                                           cTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGt                                                                                                       <  2:2656712/150‑1 (MQ=255)
                                           cTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGt                                                                                                       <  2:197801/150‑1 (MQ=255)
                                            tGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTg                                                                                                      <  2:495648/150‑1 (MQ=255)
                                                tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                                  <  2:457975/150‑1 (MQ=255)
                                                tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                                  >  1:1062499/1‑150 (MQ=255)
                                                tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                                  >  1:3991290/1‑150 (MQ=255)
                                                 ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGa                                                                                                 <  2:2871135/150‑1 (MQ=255)
                                                    cTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTc                                                                                              >  1:3325362/1‑150 (MQ=255)
                                                     tGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCa                                                                                             >  1:4241670/1‑150 (MQ=255)
                                                     tGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCa                                                                                             >  2:3008624/1‑150 (MQ=255)
                                                      gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag                                                                                            >  2:4186673/1‑150 (MQ=255)
                                                      gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag                                                                                            >  1:1502834/1‑150 (MQ=255)
                                                      gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag                                                                                            >  2:2871977/1‑150 (MQ=255)
                                                          cAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                        >  1:4457570/1‑150 (MQ=255)
                                                          cAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                        <  2:3452181/150‑1 (MQ=255)
                                                           aGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGc                                                                                       <  1:346653/150‑1 (MQ=255)
                                                            gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                      >  2:274480/1‑150 (MQ=255)
                                                            gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                      <  1:3355105/150‑1 (MQ=255)
                                                                caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCa                                                                                  >  2:954332/1‑150 (MQ=255)
                                                                 accaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAg                                                                                 <  1:504974/150‑1 (MQ=255)
                                                                  ccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGt                                                                                >  2:1369927/1‑150 (MQ=255)
                                                                  ccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGt                                                                                <  1:1950418/150‑1 (MQ=255)
                                                                   caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                               >  1:1464876/1‑150 (MQ=255)
                                                                   caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                               <  1:531931/150‑1 (MQ=255)
                                                                       aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATa                                                                           <  2:4082316/150‑1 (MQ=255)
                                                                          aCTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAAc                                                                        >  1:213017/1‑150 (MQ=255)
                                                                            ttCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTg                                                                      >  1:2317760/1‑150 (MQ=255)
                                                                               cGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTc                                                                   >  1:3427180/1‑150 (MQ=255)
                                                                               cGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTc                                                                   <  1:2972528/150‑1 (MQ=255)
                                                                               cGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTc                                                                   <  1:2642732/150‑1 (MQ=255)
                                                                                ggTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCg                                                                  <  2:3251511/150‑1 (MQ=255)
                                                                                ggTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCg                                                                  <  2:4328157/150‑1 (MQ=255)
                                                                                 gTGCCGGGTTGTCGATCACCAGCGAGCAGCTACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCgg                                                                 >  1:1410350/1‑150 (MQ=255)
                                                                                      gggTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaata                                                            <  2:802514/150‑1 (MQ=255)
                                                                                      gggTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaata                                                            <  2:3082877/150‑1 (MQ=255)
                                                                                        gTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATaaa                                                          <  2:658466/150‑1 (MQ=255)
                                                                                            tCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGcc                                                      <  2:392151/150‑1 (MQ=255)
                                                                                              gATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCtt                                                    >  1:3836851/1‑150 (MQ=255)
                                                                                                   ccAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCg                                               <  1:4050641/150‑1 (MQ=255)
                                                                                                    cAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCgg                                              <  1:1392426/150‑1 (MQ=255)
                                                                                                          gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt                                        <  1:2464703/150‑1 (MQ=255)
                                                                                                          gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt                                        >  2:2741155/1‑150 (MQ=255)
                                                                                                          gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTGGTTCGGCACGAt                                        >  2:142967/1‑150 (MQ=255)
                                                                                                            agcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                      <  1:3614347/150‑1 (MQ=255)
                                                                                                              cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc                                    <  2:383184/150‑1 (MQ=255)
                                                                                                                      tGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACg                            <  2:2134604/150‑1 (MQ=255)
                                                                                                                        cGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                          >  2:2199219/1‑150 (MQ=255)
                                                                                                                        cGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                          <  2:1575921/150‑1 (MQ=255)
                                                                                                                        cGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                          <  2:1087236/150‑1 (MQ=255)
                                                                                                                         ggTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTc                         >  1:1795854/1‑150 (MQ=255)
                                                                                                                          gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTcc                        <  1:2159190/150‑1 (MQ=255)
                                                                                                                           tGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTccc                       >  1:115671/1‑150 (MQ=255)
                                                                                                                           tGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTccc                       >  1:101957/1‑150 (MQ=255)
                                                                                                                            gAGCAGGATTTCCGTCACTTCTTAGCTATTGGCTTCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCa                      <  2:3156517/150‑1 (MQ=255)
                                                                                                                            gAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCa                      <  1:3263995/150‑1 (MQ=255)
                                                                                                                              gCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGt                    <  2:87773/150‑1 (MQ=255)
                                                                                                                                aGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCg                  >  1:1675468/1‑150 (MQ=255)
                                                                                                                                 ggATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAACAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGc                 >  2:892984/1‑150 (MQ=255)
                                                                                                                                   aTTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg               <  2:3615602/150‑1 (MQ=255)
                                                                                                                                      tCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtg            <  1:623548/150‑1 (MQ=255)
                                                                                                                                        cGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt          <  2:2434756/150‑1 (MQ=255)
                                                                                                                                         gTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAGCGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtg         >  2:4519833/1‑150 (MQ=255)
                                                                                                                                         gTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtg         >  2:2317099/1‑150 (MQ=255)
                                                                                                                                               tcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgcg   <  1:2885516/150‑1 (MQ=255)
                                                                                                                                               tcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgcg   <  1:3092190/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCGC  >  CP009273/4376031‑4376324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑