BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,394,391	G→T	7.0%	T63T (ACG→ACT)	yjeT →	DUF2065 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,394,391	0	G	T	7.0%	926.2 / 29.0	250	T63T (ACG→ACT)	yjeT	DUF2065 family protein
Reads supporting (aligned to +/- strand): ref base G (117/115); new base T (8/10); total (125/125)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394243‑4394540

 aCTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTTTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAt                                                                                                                                                     >  1:4408241/1‑150 (MQ=255)

 aCTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAt                                                                                                                                                     >  2:2614345/1‑150 (MQ=255)

 aCTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAt                                                                                                                                                     >  2:2615882/1‑150 (MQ=255)

 aCTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAt                                                                                                                                                     >  1:2567400/1‑150 (MQ=255)

     gAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGc                                                                                                                                                 >  2:706253/1‑150 (MQ=255)

     gAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGc                                                                                                                                                 >  2:2987075/1‑150 (MQ=255)

       aGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTg                                                                                                                                               <  1:126994/150‑1 (MQ=255)

            tAGGGCCTATGCTTTACCCTAAGGCATGGAAGAAGATGATCTCTGAGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGAGGTGGACTTGTGGTTGAGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGATGAACaa                                                                                                                                          >  2:2684939/1‑150 (MQ=255)

              gggCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACaaaa                                                                                                                                        >  1:4357267/1‑150 (MQ=255)

               ggCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaaaa                                                                                                                                       >  1:1640208/1‑150 (MQ=255)

                  cGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACa                                                                                                                                    <  1:4531499/150‑1 (MQ=255)

                  cGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACa                                                                                                                                    >  2:3897153/1‑150 (MQ=255)

                     tGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGAc                                                                                                                                 <  1:384484/150‑1 (MQ=255)

                     tGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGAc                                                                                                                                 <  1:386038/150‑1 (MQ=255)

                     tGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGAc                                                                                                                                 <  1:2192104/150‑1 (MQ=255)

                         ttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGAt                                                                                                                             >  1:113033/1‑150 (MQ=255)

                            cccGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGa                                                                                                                          >  1:2942081/1‑150 (MQ=255)

                             ccGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAg                                                                                                                         <  2:1420744/150‑1 (MQ=255)

                             ccGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATGTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAg                                                                                                                         <  2:3684975/150‑1 (MQ=255)

                               gAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGt                                                                                                                       >  1:589198/1‑150 (MQ=255)

                               gAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGACGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTAGTGGTCTACTACATGTTGAGTAAAACGATTGGCTGAACAAAAAACAGACTGATAGAGGt                                                                                                                       >  1:872244/1‑150 (MQ=255)

                                aaGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTc                                                                                                                      >  2:427642/1‑150 (MQ=255)

                                 aGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCTGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCa                                                                                                                     >  1:4451675/1‑150 (MQ=255)

                                  ggCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAt                                                                                                                    <  1:1092092/150‑1 (MQ=255)

                                   gCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtt                                                                                                                   <  2:1482375/150‑1 (MQ=255)

                                    cATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttt                                                                                                                  <  2:3773458/150‑1 (MQ=255)

                                    cATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttt                                                                                                                  <  2:2143222/150‑1 (MQ=255)

                                    cATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttt                                                                                                                  <  1:3182336/150‑1 (MQ=255)

                                     aTGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtttt                                                                                                                 <  2:2718841/150‑1 (MQ=255)

                                     aTGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtttt                                                                                                                 <  1:1375123/150‑1 (MQ=255)

                                     aTGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtttt                                                                                                                 >  1:21037/1‑150 (MQ=255)

                                      tGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttttt                                                                                                                >  2:2316515/1‑150 (MQ=255)

                                      tGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttttt                                                                                                                >  2:4086747/1‑150 (MQ=255)

                                      tGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttttt                                                                                                                >  2:924473/1‑150 (MQ=255)

                                      tGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAttttt                                                                                                                <  1:1437119/150‑1 (MQ=255)

                                         aagaagATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAg                                                                                                             >  2:1554555/1‑150 (MQ=255)

                                          agaagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGt                                                                                                            >  2:248762/1‑150 (MQ=255)

                                          agaagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGt                                                                                                            >  2:294786/1‑150 (MQ=255)

                                          agaagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGt                                                                                                            >  2:292664/1‑150 (MQ=255)

                                              gatgatCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCaa                                                                                                        >  1:4245455/1‑150 (MQ=255)

                                              gatgatCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCaa                                                                                                        >  2:1356571/1‑150 (MQ=255)

                                                  atCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAg                                                                                                    >  1:1999333/1‑150 (MQ=255)

                                                    ctctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTg                                                                                                  >  1:3477231/1‑150 (MQ=255)

                                                     tctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGc                                                                                                 <  2:4352157/150‑1 (MQ=255)

                                                      ctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCt                                                                                                <  1:3411385/150‑1 (MQ=255)

                                                      ctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCt                                                                                                <  1:2618475/150‑1 (MQ=255)

                                                         cGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTa                                                                                             <  1:4529081/150‑1 (MQ=255)

                                                          gATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTaa                                                                                            >  2:465120/1‑150 (MQ=255)

                                                              aCCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActct                                                                                        >  1:3760189/1‑150 (MQ=255)

                                                              aCCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActct                                                                                        >  1:698748/1‑150 (MQ=255)

                                                                cAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGa                                                                                      <  1:2284681/150‑1 (MQ=255)

                                                                     tGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAg                                                                                 <  2:1755514/150‑1 (MQ=255)

                                                                     ggCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAg                                                                                 <  1:2439661/149‑1 (MQ=255)

                                                                        ccGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGa                                                                              <  1:3131703/150‑1 (MQ=255)

                                                                         agATAATATTTTTAGTCGTTTTGGCTGTGGACTTGTGGTTGCGGGCGTTGTGGTCTTCTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGAt                                                                             <  1:324354/149‑1 (MQ=255)

                                                                            taataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGt                                                                          >  1:1006082/1‑150 (MQ=255)

                                                                            taataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGt                                                                          <  1:593393/150‑1 (MQ=255)

                                                                                    tACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCa                                                                  >  1:1671044/1‑150 (MQ=255)

                                                                                      cgtcgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAtt                                                                >  2:1866859/1‑150 (MQ=255)

                                                                                         cgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAttttt                                                             <  2:1919710/150‑1 (MQ=255)

                                                                                         cgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAttttt                                                             <  2:1956470/150‑1 (MQ=255)

                                                                                          gtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTa                                                            >  1:4171142/1‑150 (MQ=255)

                                                                                          gtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTa                                                            >  1:4171168/1‑150 (MQ=255)

                                                                                           ttttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTaa                                                           >  2:4126610/1‑150 (MQ=255)

                                                                                           ttttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTaa                                                           <  2:4022335/150‑1 (MQ=255)

                                                                                           ttttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTaa                                                           >  1:248869/1‑150 (MQ=255)

                                                                                             ttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGc                                                         >  1:3908560/1‑150 (MQ=255)

                                                                                             ttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGc                                                         <  2:1374117/150‑1 (MQ=255)

                                                                                               ggCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCaa                                                       <  2:2283966/150‑1 (MQ=255)

                                                                                               ggCGGTGGACATGTGGTAGCTGGCGTTGTGGTCTACTACATGTTTAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGATGTCATTTTTGAGTGCAAAAAGTGATGTAACTCTGAAAAAGCGATGTTAGAATCCATTTTTAAGCaa                                                       >  2:2908203/1‑150 (MQ=255)

                                                                                                 cGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAAc                                                     <  1:3552473/150‑1 (MQ=255)

                                                                                                 cGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAAc                                                     <  1:3552250/150‑1 (MQ=255)

                                                                                                  ggTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACg                                                    >  1:3143098/1‑150 (MQ=255)

                                                                                                     ggACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTg                                                 <  1:294555/150‑1 (MQ=255)

                                                                                                     ggACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTg                                                 <  2:824507/150‑1 (MQ=255)

                                                                                                       aCTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGAt                                               <  1:2731098/150‑1 (MQ=255)

                                                                                                       aCTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGAt                                               >  2:3810752/1‑150 (MQ=255)

                                                                                                           gtgGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTg                                           <  2:589198/150‑1 (MQ=255)

                                                                                                           gtgGTTGAGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAAAAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTATCTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTg                                           <  2:872244/150‑1 (MQ=255)

                                                                                                              gTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGaaa                                        >  1:3721228/1‑150 (MQ=255)

                                                                                                                      cGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTa                                >  2:2009937/1‑150 (MQ=255)

                                                                                                                            ggTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcg                          <  2:4516248/150‑1 (MQ=255)

                                                                                                                            ggTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcg                          >  1:1337402/1‑150 (MQ=255)

                                                                                                                             gTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgt                         >  2:3106167/1‑150 (MQ=255)

                                                                                                                               ctactaCATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAATCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcg                       >  2:2668513/1‑150 (MQ=255)

                                                                                                                                tactacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgt                      <  2:2126145/150‑1 (MQ=255)

                                                                                                                                 actacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtc                     >  2:1212803/1‑150 (MQ=255)

                                                                                                                                      aTGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACt                >  2:182762/1‑150 (MQ=255)

                                                                                                                                       tGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTg               <  1:3311999/150‑1 (MQ=255)

                                                                                                                                        gTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTgg              <  1:983984/150‑1 (MQ=255)

                                                                                                                                         ttGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTggg             <  1:2045745/150‑1 (MQ=255)

                                                                                                                                          tGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGc            >  2:2817754/1‑150 (MQ=255)

                                                                                                                                           gAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCa           <  2:3650446/150‑1 (MQ=255)

                                                                                                                                              gAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAACATGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCAccc        <  2:3391566/150‑1 (MQ=255)

                                                                                                                                              gAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTAc                 <  2:3084179/141‑1 (MQ=255)

                                                                                                                                              gAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTAc                 >  1:3084179/1‑141 (MQ=255)

                                                                                                                                               aaaaCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCa       >  2:1824139/1‑150 (MQ=255)

                                                                                                                                                aaaCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTCACAACGTCGTCGTACTGGGCACCCaa      >  1:712635/1‑150 (MQ=255)

                                                                                                                                                  aCGATTGTCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATg    <  2:3308029/150‑1 (MQ=255)

                                                                                                                                                    gATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATggg  >  2:3580651/1‑150 (MQ=255)

TACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394243‑4394540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`