BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,407,017	C→T	45.4%	I83I (ATC→ATT)	yjfP →	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,407,017	0	C	T	45.4%	101.8 / inf	262	I83I (ATC→ATT)	yjfP	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base C (67/76); new base T (46/73); total (113/149)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

CACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAT  >  CP009273/4406868‑4407165

      tttACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTa                                                                                                                                                <  2:2933977/150‑1 (MQ=255)

        tACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACa                                                                                                                                              >  1:2200489/1‑150 (MQ=255)

           ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAg                                                                                                                                           >  2:330177/1‑150 (MQ=255)

           ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAg                                                                                                                                           >  1:479817/1‑150 (MQ=255)

           ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAg                                                                                                                                           <  2:528898/150‑1 (MQ=255)

            tCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGt                                                                                                                                          >  1:817010/1‑150 (MQ=255)

              aTCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTAt                                                                                                                                        <  2:2484375/150‑1 (MQ=255)

               tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATg                                                                                                                                       <  1:1033968/150‑1 (MQ=255)

                ccAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGc                                                                                                                                      >  2:2319518/1‑150 (MQ=255)

                ccAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGACGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCTGCGGTTAAATGAATTCTGGCAAATCTTGCTACAAAGTATGc                                                                                                                                      <  2:4403546/150‑1 (MQ=255)

                 cAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCa                                                                                                                                     <  1:3725294/150‑1 (MQ=255)

                  aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAg                                                                                                                                    >  1:2726907/1‑150 (MQ=255)

                  aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAg                                                                                                                                    <  2:1078398/150‑1 (MQ=255)

                  aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAg                                                                                                                                    >  2:112496/1‑150 (MQ=255)

                   gTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAgg                                                                                                                                   <  1:2654734/150‑1 (MQ=255)

                    tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGTGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                                                                                                                                  <  1:933252/150‑1 (MQ=255)

                     cTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGaa                                                                                                                                 >  2:704049/1‑150 (MQ=255)

                       ggTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                                                                                                                               <  1:3580039/150‑1 (MQ=255)

                       ggTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                                                                                                                               >  1:2331307/1‑150 (MQ=255)

                       ggTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                                                                                                                               >  1:1642940/1‑150 (MQ=255)

                           tataGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCact                                                                                                                           <  2:3940828/150‑1 (MQ=255)

                             taGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCactac                                                                                                                         <  2:772487/150‑1 (MQ=255)

                             taGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCactac                                                                                                                         <  1:573848/150‑1 (MQ=255)

                              aGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCactact                                                                                                                        >  2:1071325/1‑150 (MQ=255)

                                cTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACttt                                                                                                                      <  2:4352508/150‑1 (MQ=255)

                                 tATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTa                                                                                                                     >  1:780168/1‑150 (MQ=255)

                                      tGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGc                                                                                                                <  2:2177824/150‑1 (MQ=255)

                                      tGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGc                                                                                                                >  2:1831681/1‑150 (MQ=255)

                                      tGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGc                                                                                                                >  2:1921018/1‑150 (MQ=255)

                                       gCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCg                                                                                                               <  1:3828518/150‑1 (MQ=255)

                                          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                            >  2:119215/1‑150 (MQ=255)

                                             gcgcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATa                                                                                                         <  1:4166599/150‑1 (MQ=255)

                                               gcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGc                                                                                                       >  2:291750/1‑150 (MQ=255)

                                                cTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGcc                                                                                                      <  1:1185873/150‑1 (MQ=255)

                                                cTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGcc                                                                                                      <  1:1186109/150‑1 (MQ=255)

                                                 tGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCg                                                                                                     <  1:2777016/150‑1 (MQ=255)

                                                  ggCGCAGGCTGGTTTGCGGGTGATCATGCCGGATTCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCga                                                                                                    <  2:539850/150‑1 (MQ=255)

                                                  ggCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCga                                                                                                    <  1:3323725/150‑1 (MQ=255)

                                                    cgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                  <  2:4240063/150‑1 (MQ=255)

                                                     gcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaaga                                                                                                 <  2:3116737/150‑1 (MQ=255)

                                                      cAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaagaa                                                                                                >  2:1739225/1‑150 (MQ=255)

                                                      cAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaagaa                                                                                                >  2:1738882/1‑150 (MQ=255)

                                                       aGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGaaa                                                                                               >  2:3635686/1‑150 (MQ=255)

                                                          cTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACt                                                                                            <  1:1986511/150‑1 (MQ=255)

                                                           tGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTg                                                                                           <  1:3825954/150‑1 (MQ=255)

                                                            ggTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTgg                                                                                          <  1:2688699/150‑1 (MQ=255)

                                                             gTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgc                                                                                         >  2:2488405/1‑150 (MQ=255)

                                                               ttGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctg                                                                                       <  2:2848389/150‑1 (MQ=255)

                                                                 gCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctgct                                                                                     <  1:531681/150‑1 (MQ=255)

                                                                    ggTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtga                                                                                  >  1:1425111/1‑150 (MQ=255)

                                                                    ggTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtga                                                                                  >  2:4070722/1‑150 (MQ=255)

                                                                     gTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgat                                                                                 <  2:1495178/150‑1 (MQ=255)

                                                                      tGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgatg                                                                                >  2:1882978/1‑150 (MQ=255)

                                                                        aTCATGCCGGATGCGCCCGATCACGTTAGCCGTTTTAGTGGAGACGCAGCGCGGCGGTTAACTCAATTCTGGCAAATCTTGCTACAAATTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGATAACTGGCTGCTTGATGAc                                                                              <  2:1330834/150‑1 (MQ=255)

                                                                         tCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAcc                                                                             <  2:1535975/150‑1 (MQ=255)

                                                                           aTGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGt                                                                           <  2:361621/150‑1 (MQ=255)

                                                                             gCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCt                                                                         <  1:3674600/150‑1 (MQ=255)

                                                                                ggATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGc                                                                      >  1:3784098/1‑150 (MQ=255)

                                                                                ggATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGc                                                                      >  1:319422/1‑150 (MQ=255)

                                                                                 gATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCa                                                                     >  2:1514253/1‑150 (MQ=255)

                                                                                  aTGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAg                                                                    <  2:2957747/150‑1 (MQ=255)

                                                                                  aTGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAg                                                                    <  2:455000/150‑1 (MQ=255)

                                                                                               cGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTc                                                       <  1:1305240/150‑1 (MQ=255)

                                                                                                ggTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCg                                                      <  1:3850782/150‑1 (MQ=255)

                                                                                                   aGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATg                                                   >  1:1848951/1‑150 (MQ=255)

                                                                                                   aGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATg                                                   >  2:2512947/1‑150 (MQ=255)

                                                                                                   aGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATg                                                   >  2:2623030/1‑150 (MQ=255)

                                                                                                      cGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                                <  1:2572794/150‑1 (MQ=255)

                                                                                                           tAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGAt                                           <  1:2749417/150‑1 (MQ=255)

                                                                                                            aGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATg                                          <  2:780168/150‑1 (MQ=255)

                                                                                                                  gACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCa                                    <  2:1993813/150‑1 (MQ=255)

                                                                                                                  gACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCa                                    <  2:176812/150‑1 (MQ=255)

                                                                                                                  gACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCa                                    <  2:861938/150‑1 (MQ=255)

                                                                                                                    cGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACt                                  <  1:286281/150‑1 (MQ=255)

                                                                                                                    cGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACt                                  <  2:2994623/150‑1 (MQ=255)

                                                                                                                        gcgcGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTgggg                              <  2:2363378/150‑1 (MQ=255)

                                                                                                                         cgcgGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGa                             <  2:578055/150‑1 (MQ=255)

                                                                                                                         cgcgGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGa                             >  1:2883240/1‑150 (MQ=255)

                                                                                                                            ggcggTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTa                          <  1:110511/150‑1 (MQ=255)

                                                                                                                                  tAACTCAATTCTGGCAAATTTTGCTACAACGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTc                    <  2:2648406/150‑1 (MQ=255)

                                                                                                                                  tAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTc                    >  1:1887002/1‑150 (MQ=255)

                                                                                                                                  tAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTc                    <  1:2359220/150‑1 (MQ=255)

                                                                                                                                   aaaTCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCg                   <  2:4136339/150‑1 (MQ=255)

                                                                                                                                   aaaTCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCg                   >  2:3823719/1‑150 (MQ=255)

                                                                                                                                     aTCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGcc                 >  1:3949057/1‑150 (MQ=255)

                                                                                                                                          ttCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAcccc            <  1:2949889/150‑1 (MQ=255)

                                                                                                                                           tCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCa           <  1:571818/150‑1 (MQ=255)

                                                                                                                                              ggCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACgg        <  1:3817794/150‑1 (MQ=255)

                                                                                                                                              ggCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACgg        <  1:3817899/150‑1 (MQ=255)

                                                                                                                                              ggCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACgg        <  1:1555367/150‑1 (MQ=255)

                                                                                                                                              ggCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACgg        >  2:4390171/1‑150 (MQ=255)

                                                                                                                                                 aaaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTga     >  1:1051209/1‑150 (MQ=255)

                                                                                                                                                 aaaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTga     >  1:2516705/1‑150 (MQ=255)

                                                                                                                                                  aaTTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    <  1:1933987/150‑1 (MQ=255)

                                                                                                                                                  aaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    >  2:1344980/1‑150 (MQ=255)

                                                                                                                                                  aaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag    >  1:1806205/1‑150 (MQ=255)

                                                                                                                                                   aTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgaga   >  1:945866/1‑150 (MQ=255)

                                                                                                                                                   aTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgaga   >  2:416644/1‑150 (MQ=255)

                                                                                                                                                    tCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAt  >  2:2290799/1‑150 (MQ=255)

                                                                                                                                                    tCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGAGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAt  <  1:4070783/150‑1 (MQ=255)

CACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGAT  >  CP009273/4406868‑4407165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`