Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,428,092 G→T 72.3% G190V (GGA→GTA)  ytfI → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,428,0920GT72.3% 327.6 / 233.3 253G190V (GGA→GTA) ytfIuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (27/43);  new base T (97/86);  total (124/129)
Fisher's exact test for biased strand distribution p-value = 4.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 260 total aligned reads displayed.

GATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAG  >  CP009273/4427944‑4428240
                                                                                                                                                    |                                                                                                                                                    
 atatTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCaggag                                                                                                                                                    <  1:4003032/150‑1 (MQ=255)
       tACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGaa                                                                                                                                              >  2:44731/1‑150 (MQ=255)
        aCAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAAt                                                                                                                                             >  2:940292/1‑150 (MQ=255)
         cAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAAtt                                                                                                                                            <  1:507198/150‑1 (MQ=255)
          aGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTa                                                                                                                                           <  1:1226048/150‑1 (MQ=255)
           gAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTaa                                                                                                                                          <  2:595992/150‑1 (MQ=255)
             aaaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAg                                                                                                                                        <  2:1779076/150‑1 (MQ=255)
                aaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAAt                                                                                                                                     <  1:4258298/150‑1 (MQ=255)
                   aGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCg                                                                                                                                  <  2:2723353/150‑1 (MQ=255)
                   aGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCg                                                                                                                                  >  1:1803365/1‑150 (MQ=255)
                     tCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgat                                                                                                                                <  2:4150109/150‑1 (MQ=255)
                      cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatg                                                                                                                               >  1:1134450/1‑150 (MQ=255)
                      cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatg                                                                                                                               >  2:4227725/1‑150 (MQ=255)
                       tGACATACCGAAAATGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatgg                                                                                                                              <  2:3342030/150‑1 (MQ=255)
                        gACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatgga                                                                                                                             >  2:3140534/1‑150 (MQ=255)
                         aCATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatggat                                                                                                                            >  2:2292745/1‑150 (MQ=255)
                              ccGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGtat                                                                                                                       >  1:2117634/1‑150 (MQ=255)
                               cGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGtata                                                                                                                      <  1:1697954/150‑1 (MQ=255)
                               cGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGtata                                                                                                                      <  1:3658804/150‑1 (MQ=255)
                                aaaaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTatat                                                                                                                     >  1:274929/2‑150 (MQ=255)
                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                  <  2:2428692/150‑1 (MQ=255)
                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                  <  1:3012994/150‑1 (MQ=255)
                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                  <  2:1533909/150‑1 (MQ=255)
                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                  <  2:38692/150‑1 (MQ=255)
                                     ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACg                                                                                                                <  1:255/150‑1 (MQ=255)
                                     ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACg                                                                                                                <  1:196/150‑1 (MQ=255)
                                       aTTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                              >  2:4172063/1‑150 (MQ=255)
                                       aTTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                              >  2:2563764/1‑150 (MQ=255)
                                         tAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaaaa                                                                                                            >  2:637692/1‑150 (MQ=255)
                                         tAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaaaa                                                                                                            <  1:3490046/150‑1 (MQ=255)
                                           aaGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGAAATTAAAGAATGCGATGGATGGTATATTTACGaaaaaa                                                                                                          >  1:2347728/1‑150 (MQ=255)
                                               ttATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaataat                                                                                                      <  2:2130749/150‑1 (MQ=255)
                                                tataTTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAAtt                                                                                                     <  2:2948413/150‑1 (MQ=255)
                                                   atTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgat                                                                                                  >  1:3293499/1‑150 (MQ=255)
                                                   atTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgat                                                                                                  >  2:2555588/1‑150 (MQ=255)
                                                     tCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgatga                                                                                                >  1:3430652/1‑150 (MQ=255)
                                                      cACCAGGCGATTGGTGACGTGTCTTTAGGTTATGACTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTgatgat                                                                                               <  2:4054249/150‑1 (MQ=255)
                                                        ccAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATaa                                                                                             <  1:1005320/150‑1 (MQ=255)
                                                         cAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAt                                                                                            <  1:588325/150‑1 (MQ=255)
                                                           ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          <  2:1036106/150‑1 (MQ=255)
                                                           ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          <  2:1134450/150‑1 (MQ=255)
                                                           ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          >  1:924382/1‑150 (MQ=255)
                                                           ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          >  1:1283123/1‑150 (MQ=255)
                                                            gCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtttt                                                                                         <  1:1439293/150‑1 (MQ=255)
                                                            gCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtttt                                                                                         <  1:1370143/150‑1 (MQ=255)
                                                             cGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTg                                                                                        <  1:3749213/150‑1 (MQ=255)
                                                              gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                       <  2:3743889/150‑1 (MQ=255)
                                                              gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATTCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                       >  2:744190/1‑150 (MQ=255)
                                                              gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                       <  2:4225542/150‑1 (MQ=255)
                                                                ttGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCa                                                                                     <  1:2575038/150‑1 (MQ=255)
                                                                ttGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCa                                                                                     <  2:217979/150‑1 (MQ=255)
                                                                  ggTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATa                                                                                   <  2:2061338/150‑1 (MQ=255)
                                                                   gTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAg                                                                                  <  2:4352146/150‑1 (MQ=255)
                                                                    tGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                                 <  1:2653835/150‑1 (MQ=255)
                                                                    tGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                                 <  1:3336531/150‑1 (MQ=255)
                                                                     gACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTc                                                                                >  1:3517867/1‑150 (MQ=255)
                                                                            cTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAAt                                                                         >  2:3397769/1‑150 (MQ=255)
                                                                            cTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAAt                                                                         <  1:3091389/150‑1 (MQ=255)
                                                                             tttAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATc                                                                        <  1:2292745/150‑1 (MQ=255)
                                                                             tttAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATc                                                                        <  1:3417540/150‑1 (MQ=255)
                                                                              ttAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCt                                                                       <  2:4396935/150‑1 (MQ=255)
                                                                               tAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCtt                                                                      <  1:105863/150‑1 (MQ=255)
                                                                               tAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCtt                                                                      <  1:1940049/150‑1 (MQ=255)
                                                                                 ggTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTg                                                                    <  1:1899227/150‑1 (MQ=255)
                                                                                  gTTATGCCTTATTGGAGGATATAGTAAGCAAATTTAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTgg                                                                   <  2:179834/150‑1 (MQ=255)
                                                                                  gTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTgg                                                                   <  1:4111344/150‑1 (MQ=255)
                                                                                   ttATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTggg                                                                  <  2:3870523/150‑1 (MQ=255)
                                                                                       gCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCttt                                                              >  2:3950629/1‑150 (MQ=255)
                                                                                          ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                           >  2:4384341/1‑150 (MQ=255)
                                                                                          ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                           >  2:1985279/1‑150 (MQ=255)
                                                                                          ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                           <  1:1312295/150‑1 (MQ=255)
                                                                                              tGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTa                                                       >  2:4343779/1‑150 (MQ=255)
                                                                                               ggaggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaa                                                      <  2:3117366/150‑1 (MQ=255)
                                                                                                 aggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACAAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAAAAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGATTTGCGCTTaaaa                                                    >  1:3052318/1‑150 (MQ=255)
                                                                                                        aGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATgg                                             >  2:747225/1‑150 (MQ=255)
                                                                                                           aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTTATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgg                                          >  1:802157/1‑150 (MQ=255)
                                                                                                             gCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgggg                                        >  1:2215651/1‑150 (MQ=255)
                                                                                                                aaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATg                                     >  1:1533361/1‑150 (MQ=255)
                                                                                                                aaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATg                                     >  2:1283685/1‑150 (MQ=255)
                                                                                                                 aTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGa                                    >  2:2750578/1‑150 (MQ=255)
                                                                                                                  ttAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGaa                                   <  2:1170680/150‑1 (MQ=255)
                                                                                                                    aaaaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACg                                 <  1:211972/150‑1 (MQ=255)
                                                                                                                     aaaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGt                                >  2:3008094/1‑150 (MQ=255)
                                                                                                                      aaCGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGtt                               <  1:704253/150‑1 (MQ=255)
                                                                                                                        cGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGtttt                             >  2:3266205/1‑150 (MQ=255)
                                                                                                                         ggggTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                            >  2:2631848/1‑150 (MQ=255)
                                                                                                                             tAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTaa                        >  2:1416827/1‑150 (MQ=255)
                                                                                                                                aTTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                     >  1:1758444/1‑150 (MQ=255)
                                                                                                                                 tttGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGt                    >  1:794383/1‑150 (MQ=255)
                                                                                                                                  ttGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGtt                   >  2:1578050/1‑150 (MQ=255)
                                                                                                                                     aaCTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCct                >  2:4158160/1‑150 (MQ=255)
                                                                                                                                      aCTCCAAATACCAGTAGGTTGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctc               <  1:2787858/150‑1 (MQ=255)
                                                                                                                                        tCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg             >  1:1618074/1‑150 (MQ=255)
                                                                                                                                         ccAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgg            >  2:3079125/1‑149 (MQ=255)
                                                                                                                                         ccAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgt            <  1:4037113/150‑1 (MQ=255)
                                                                                                                                          cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt           >  1:3942915/1‑150 (MQ=255)
                                                                                                                                          cAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt           <  1:3095698/150‑1 (MQ=255)
                                                                                                                                           aaaTACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgttg          >  2:2555283/1‑150 (MQ=255)
                                                                                                                                                ccAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTg     <  1:4172063/150‑1 (MQ=255)
                                                                                                                                                  aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga   >  1:1128223/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAG  >  CP009273/4427944‑4428240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑