Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,460,919 T→A 5.3% S116C (AGC→TGC)  pyrI ← aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,460,9190TA 5.3% 827.2 / 26.2 245S116C (AGC→TGC) pyrIaspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (124/108);  new base A (6/7);  total (130/115)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 246 total aligned reads displayed.

TATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACT  >  CP009273/4460770‑4461066
                                                                                                                                                     |                                                                                                                                                   
  taGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa                                                                                                                                                   <  2:1012352/150‑1 (MQ=255)
   aGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGAt                                                                                                                                                  >  2:4047951/1‑150 (MQ=255)
      gAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACa                                                                                                                                               <  2:2025009/150‑1 (MQ=255)
         ccAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGtt                                                                                                                                            >  1:1070304/1‑150 (MQ=255)
          cAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTg                                                                                                                                           <  1:1436152/150‑1 (MQ=255)
             aCTTTTATTACCAACCTCAATTAATTGGCCAGCAACACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTg                                                                                                                                        <  2:2914058/150‑1 (MQ=255)
             aCTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTg                                                                                                                                        <  2:631985/150‑1 (MQ=255)
                tttATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTc                                                                                                                                     >  2:2697389/1‑150 (MQ=255)
                   attaCCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCggg                                                                                                                                  >  1:4509248/1‑150 (MQ=255)
                    ttaCCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGc                                                                                                                                 >  2:2167496/1‑150 (MQ=255)
                    ttaCCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGc                                                                                                                                 <  1:3988022/150‑1 (MQ=255)
                    ttaCCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGc                                                                                                                                 <  1:2245332/150‑1 (MQ=255)
                       ccAACCTCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTTTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGa                                                                                                                              <  1:2014727/150‑1 (MQ=255)
                         aaCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGAcc                                                                                                                            <  1:1332749/150‑1 (MQ=255)
                         aaCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCAGATACAGTTGCTGTTCGGGCAGAcc                                                                                                                            <  2:490395/150‑1 (MQ=255)
                         aaCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCAGATACAGTTGCTGTTCGGGCAGAcc                                                                                                                            >  2:1557998/1‑150 (MQ=255)
                              cAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGcac                                                                                                                       <  1:730737/150‑1 (MQ=255)
                              cAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGcac                                                                                                                       <  1:3010697/150‑1 (MQ=255)
                              cAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGcac                                                                                                                       >  1:2371175/1‑150 (MQ=255)
                               aattaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGcaca                                                                                                                      >  2:896948/1‑150 (MQ=255)
                                attaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACAt                                                                                                                     <  2:3795478/150‑1 (MQ=255)
                                attaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACAt                                                                                                                     >  1:3798703/1‑150 (MQ=255)
                                 ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACAtt                                                                                                                    >  1:1592636/1‑150 (MQ=255)
                                   aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGt                                                                                                                  >  2:24800/1‑150 (MQ=255)
                                    attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTc                                                                                                                 <  2:3786076/150‑1 (MQ=255)
                                    attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTc                                                                                                                 <  1:1058226/150‑1 (MQ=255)
                                    attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTc                                                                                                                 <  1:1321912/150‑1 (MQ=255)
                                       ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGAt                                                                                                              >  2:3001906/1‑150 (MQ=255)
                                        gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATg                                                                                                             <  2:2462527/150‑1 (MQ=255)
                                         ccAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATgc                                                                                                            <  1:2557845/150‑1 (MQ=255)
                                            gCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCt                                                                                                         <  1:412450/150‑1 (MQ=255)
                                            gCAACACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCt                                                                                                         <  1:414095/150‑1 (MQ=255)
                                             caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTc                                                                                                        >  1:1893463/1‑150 (MQ=255)
                                              accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTcc                                                                                                       >  2:1824519/1‑150 (MQ=255)
                                               ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCg                                                                                                      >  2:1902515/1‑150 (MQ=255)
                                                cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTTATCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGTTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATAGTCGATGCGCTCCgg                                                                                                     >  2:3995579/1‑150 (MQ=255)
                                                    ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGa                                                                                                 <  1:625043/150‑1 (MQ=255)
                                                           aaaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGgc                                                                                          >  2:1134113/1‑150 (MQ=255)
                                                             aaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGgcgc                                                                                        >  2:724312/1‑150 (MQ=255)
                                                              aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGcgcg                                                                                       >  2:3189320/1‑150 (MQ=255)
                                                              aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGcgcg                                                                                       >  1:4047094/1‑150 (MQ=255)
                                                               ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGa                                                                                      >  2:3652239/1‑150 (MQ=255)
                                                                 ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGAtt                                                                                    <  2:2831272/150‑1 (MQ=255)
                                                                 ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGAtt                                                                                    >  1:3198844/1‑150 (MQ=255)
                                                                  tttttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGAttt                                                                                   <  1:1126062/150‑1 (MQ=255)
                                                                  tttttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCAGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGAttt                                                                                   >  2:3996490/1‑150 (MQ=255)
                                                                    tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTAc                                                                                 >  2:3237169/1‑150 (MQ=255)
                                                                     ttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTAcc                                                                                >  2:288358/1‑150 (MQ=255)
                                                                       cacaGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACcca                                                                              >  2:1332218/1‑150 (MQ=255)
                                                                        acaGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccac                                                                             >  2:2555021/1‑150 (MQ=255)
                                                                         caGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTAcccccc                                                                            <  1:1529209/150‑1 (MQ=255)
                                                                         caGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccacc                                                                            <  1:1529252/150‑1 (MQ=255)
                                                                          aGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccacca                                                                           <  2:355077/150‑1 (MQ=255)
                                                                          aGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccacca                                                                           >  2:3973008/1‑150 (MQ=255)
                                                                          aGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccacca                                                                           >  2:1699482/1‑150 (MQ=255)
                                                                            tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACt                                                                         >  1:3373912/1‑150 (MQ=255)
                                                                            tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACt                                                                         <  2:4006593/150‑1 (MQ=255)
                                                                             aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACtt                                                                        >  2:3133009/1‑150 (MQ=255)
                                                                              tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTc                                                                       >  1:3330892/1‑150 (MQ=255)
                                                                               ttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCa                                                                      <  2:2332462/150‑1 (MQ=255)
                                                                                        aGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCg                                                             >  2:1679104/1‑150 (MQ=255)
                                                                                           gcgATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATa                                                          >  1:4241390/1‑150 (MQ=255)
                                                                                             gATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACg                                                        >  1:1384373/1‑150 (MQ=255)
                                                                                                atCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGtt                                                     <  2:3531969/150‑1 (MQ=255)
                                                                                                     tGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCg                                                >  1:3707076/1‑150 (MQ=255)
                                                                                                     tGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTAACCCACCACTTCATAGTTGTCGATACGGTTAAcag                                                >  1:2913983/1‑148 (MQ=255)
                                                                                                       gcgcGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTg                                              <  1:2453429/150‑1 (MQ=255)
                                                                                                          cgTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCt                                           >  1:1684381/1‑150 (MQ=255)
                                                                                                           gTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                          <  1:1049411/150‑1 (MQ=255)
                                                                                                           gTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                          <  1:3873926/150‑1 (MQ=255)
                                                                                                           gTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                          <  1:4496759/150‑1 (MQ=255)
                                                                                                                 gCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggcg                                    >  1:1338446/1‑150 (MQ=255)
                                                                                                                  cACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGgcgc                                   >  1:2870201/1‑150 (MQ=255)
                                                                                                                   aCGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCa                                  >  1:2664364/1‑150 (MQ=255)
                                                                                                                      gCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATAc                               >  1:1383262/1‑150 (MQ=255)
                                                                                                                      gCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATAc                               <  1:3623619/150‑1 (MQ=255)
                                                                                                                        aaaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACaa                             >  1:3172736/1‑150 (MQ=255)
                                                                                                                         aaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAAt                            <  2:2220189/150‑1 (MQ=255)
                                                                                                                         aaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAAt                            >  1:327756/1‑150 (MQ=255)
                                                                                                                          aGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATg                           <  2:4540299/150‑1 (MQ=255)
                                                                                                                            cTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGcc                         >  1:1581054/1‑150 (MQ=255)
                                                                                                                             tGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCa                        <  1:2584057/150‑1 (MQ=255)
                                                                                                                                 tGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTg                    <  1:2707660/150‑1 (MQ=255)
                                                                                                                                  gAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGa                   <  2:3950150/150‑1 (MQ=255)
                                                                                                                                  gAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGa                   >  2:4265899/1‑150 (MQ=255)
                                                                                                                                   aaaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGAt                  >  2:4380827/1‑150 (MQ=255)
                                                                                                                                    taCCGGTTCGGCATGTCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTTTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACGGTGGCTTGCGGCGCATACAATGCATGTTGATc                 <  2:4332560/149‑1 (MQ=255)
                                                                                                                                    aaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATc                 >  2:1220782/1‑150 (MQ=255)
                                                                                                                                    aaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATc                 >  1:85163/1‑150 (MQ=255)
                                                                                                                                       cGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTAc              <  2:2096522/150‑1 (MQ=255)
                                                                                                                                        ggTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACt             <  2:573566/150‑1 (MQ=255)
                                                                                                                                        ggTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACt             >  2:2659395/1‑150 (MQ=255)
                                                                                                                                        ggTTCGGCATGGCAGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACt             >  1:4464447/1‑150 (MQ=255)
                                                                                                                                         gTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACtt            <  2:1809941/150‑1 (MQ=255)
                                                                                                                                         gTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTAATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACtt            <  1:2959248/150‑1 (MQ=255)
                                                                                                                                                aTGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTc     <  1:675858/150‑1 (MQ=255)
                                                                                                                                                aTGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTAATCTTc     >  2:2394259/1‑150 (MQ=255)
                                                                                                                                                   gCTGATACAGTTGCTGTTCGGGCAGACCTGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCTCTTCATCGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACt  <  1:247797/150‑1 (MQ=255)
                                                                                                                                                   gCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACt  <  1:139040/150‑1 (MQ=255)
                                                                                                                                                   gCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACt  <  1:4101058/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACT  >  CP009273/4460770‑4461066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑