BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,462,966	A→T	8.5%	Y21N (TAT→AAT)	yjgH ←	UPF0131 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,462,966	0	A	T	8.5%	943.3 / 58.1	293	Y21N (TAT→AAT)	yjgH	UPF0131 family protein
Reads supporting (aligned to +/- strand): ref base A (145/123); new base T (11/14); total (156/137)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 298 total aligned reads displayed.

AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115

    cATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGa                                                                                                                                                   <  2:3998814/150‑1 (MQ=255)

    cATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGa                                                                                                                                                   <  2:2268620/150‑1 (MQ=255)

     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  <  2:4303890/150‑1 (MQ=255)

     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  >  2:37596/1‑150 (MQ=255)

     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  >  1:3200762/1‑150 (MQ=255)

      tCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATg                                                                                                                                                 >  2:1112116/1‑150 (MQ=255)

       cccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGATCGACTAGCCGCAGAAGATCGAGGc                                                                                                                                                >  1:2903890/1‑150 (MQ=255)

       cccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGc                                                                                                                                                >  2:4240419/1‑150 (MQ=255)

        ccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCt                                                                                                                                               >  1:1190951/1‑150 (MQ=255)

        ccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCt                                                                                                                                               >  1:3446903/1‑150 (MQ=255)

           cAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAg                                                                                                                                            <  1:3933789/150‑1 (MQ=255)

           cAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAg                                                                                                                                            <  2:2369062/150‑1 (MQ=255)

            aGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGc                                                                                                                                           >  1:3907472/1‑150 (MQ=255)

             gCTGCCAGGTTCTAATGAAAATTATCATATGCCAGTCTGTCTTGTTGCTTATAATCGGGTTCTGTTGTTCCGTCCTCTCGACTTCCTTCTTGCCCGGAAACAAACAGCATCTCGCCGGATCGATTATGCGCATATTAACGATGCTCAGCa                                                                                                                                          <  2:1074596/150‑1 (MQ=255)

              cTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCat                                                                                                                                         >  1:2997038/1‑150 (MQ=255)

               tGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCata                                                                                                                                        <  2:665808/150‑1 (MQ=255)

                gCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAGCAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCatat                                                                                                                                       >  2:1253933/1‑150 (MQ=255)

                   aGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGt                                                                                                                                    >  1:929887/1‑150 (MQ=255)

                    gggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTg                                                                                                                                   <  2:3612774/150‑1 (MQ=255)

                     ggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGa                                                                                                                                  <  2:1389952/150‑1 (MQ=255)

                        cGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATg                                                                                                                               >  2:4095884/1‑150 (MQ=255)

                            tGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCgg                                                                                                                           >  1:802336/1‑150 (MQ=255)

                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       >  1:2324892/1‑150 (MQ=255)

                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       >  1:2325585/1‑150 (MQ=255)

                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       >  1:2481381/1‑150 (MQ=255)

                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       >  1:1583349/1‑150 (MQ=255)

                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       <  1:4070911/150‑1 (MQ=255)

                                        aaTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaa                                                                                                               >  2:1511536/1‑150 (MQ=255)

                                        aaTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaa                                                                                                               <  1:1992250/150‑1 (MQ=255)

                                         aTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaaa                                                                                                              >  2:1303030/1‑150 (MQ=255)

                                         aTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaaa                                                                                                              >  2:2805532/1‑150 (MQ=255)

                                         aTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaaa                                                                                                              >  2:16393/1‑150 (MQ=255)

                                         aTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaaa                                                                                                              <  1:1662506/150‑1 (MQ=255)

                                         aTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaaaa                                                                                                              <  1:3148500/150‑1 (MQ=255)

                                            ccAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACa                                                                                                            >  2:2906138/1‑149 (MQ=255)

                                            ccAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAg                                                                                                           >  2:1996618/1‑150 (MQ=255)

                                             cAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGc                                                                                                          <  1:2721105/150‑1 (MQ=255)

                                              aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAACCAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  1:4236281/1‑150 (MQ=255)

                                              aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  2:572849/1‑150 (MQ=255)

                                              aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  1:1853970/1‑150 (MQ=255)

                                                 cTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTTCCCGGAAACAAACAGAAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGtt                                                                                                      >  1:210912/1‑150 (MQ=255)

                                                 cTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGtt                                                                                                      <  2:3446903/150‑1 (MQ=255)

                                                 cTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGtt                                                                                                      <  1:4533329/150‑1 (MQ=255)

                                                  tGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttc                                                                                                     <  2:915936/150‑1 (MQ=255)

                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             <  2:1789758/150‑1 (MQ=255)

                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             <  2:803395/150‑1 (MQ=255)

                                                               aaaaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTg                                                                                        >  1:4290456/1‑150 (MQ=255)

                                                               aaaaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTg                                                                                        >  1:523116/1‑150 (MQ=255)

                                                                aaaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTgg                                                                                       >  2:2692457/1‑150 (MQ=255)

                                                                 aaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGt                                                                                      >  2:1886315/1‑150 (MQ=255)

                                                                  aTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGtt                                                                                     >  2:794366/1‑150 (MQ=255)

                                                                  aTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGtt                                                                                     >  2:794398/1‑150 (MQ=255)

                                                                     gggTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATc                                                                                  <  1:737063/150‑1 (MQ=255)

                                                                      ggTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATcc                                                                                 >  1:3126321/1‑150 (MQ=255)

                                                                       gTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCt                                                                                <  1:4390192/150‑1 (MQ=255)

                                                                         tCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCa                                                                              <  2:2794839/150‑1 (MQ=255)

                                                                          cTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCaa                                                                             >  2:1162224/1‑150 (MQ=255)

                                                                           tGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAg                                                                            <  1:308123/150‑1 (MQ=255)

                                                                            ggTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTGTCTACCATTTGGTTATCCTCAAGa                                                                           >  2:2738558/1‑150 (MQ=255)

                                                                             gtgtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAt                                                                          <  1:3626875/150‑1 (MQ=255)

                                                                               gtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAttt                                                                        <  1:1503163/150‑1 (MQ=255)

                                                                                ttCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAGTTGGTTATCCTCAAGATTTa                                                                       >  1:1651103/1‑150 (MQ=255)

                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      >  2:324658/1‑150 (MQ=255)

                                                                                     tCCTCTCGACTTCCT‑CTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 >  2:4275583/1‑150 (MQ=255)

                                                                                      ccTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 <  1:2084851/150‑1 (MQ=255)

                                                                                        tctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGa                                                               <  1:2324552/150‑1 (MQ=255)

                                                                                         ctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGaa                                                              <  2:3682271/150‑1 (MQ=255)

                                                                                          tcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAAc                                                             <  2:2652015/150‑1 (MQ=255)

                                                                                          tcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAAc                                                             <  2:3369738/150‑1 (MQ=255)

                                                                                           cGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACa                                                            <  2:3630188/150‑1 (MQ=255)

                                                                                               ttCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaag                                                        <  2:2075694/150‑1 (MQ=255)

                                                                                                  cTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTTCCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGAtt                                                     <  1:3886647/150‑1 (MQ=255)

                                                                                                       tGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTctct                                                >  2:3557500/1‑150 (MQ=255)

                                                                                                        gCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCtt                                               <  1:1258267/150‑1 (MQ=255)

                                                                                                           cGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAc                                            >  2:1594256/1‑150 (MQ=255)

                                                                                                                  aaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGc                                     <  1:2010617/150‑1 (MQ=255)

                                                                                                                    acaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAACGTTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCg                                   >  1:1021109/1‑150 (MQ=255)

                                                                                                                      aGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCt                                 >  1:3118454/1‑150 (MQ=255)

                                                                                                                      aGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCt                                 >  2:104912/1‑150 (MQ=255)

                                                                                                                      aGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCt                                 >  2:109648/1‑150 (MQ=255)

                                                                                                                       gCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtt                                >  2:306397/1‑150 (MQ=255)

                                                                                                                        cAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTATCTTTACCGGGAGCCGCttt                               >  2:2929735/1‑150 (MQ=255)

                                                                                                                         aaaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtttt                              <  2:750775/150‑1 (MQ=255)

                                                                                                                            tCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGc                           <  2:374352/150‑1 (MQ=255)

                                                                                                                             cGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCg                          <  2:3907506/150‑1 (MQ=255)

                                                                                                                             cGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCg                          <  1:4219992/150‑1 (MQ=255)

                                                                                                                                 ggAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTCTCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacg                      >  2:2124423/1‑150 (MQ=255)

                                                                                                                                 ggAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacg                      >  2:4367018/1‑150 (MQ=255)

                                                                                                                                  gAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacga                     >  1:1980782/1‑150 (MQ=255)

                                                                                                                                   aaCGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacgac                    <  2:375927/150‑1 (MQ=255)

                                                                                                                                    aCGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGacgacg                   >  2:916342/1‑150 (MQ=255)

                                                                                                                                     cGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGt                  <  1:1341522/150‑1 (MQ=255)

                                                                                                                                       aaTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGa                >  1:2305361/1‑150 (MQ=255)

                                                                                                                                         tAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGt              <  1:3779149/150‑1 (MQ=255)

                                                                                                                                           gCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaa            <  1:4254791/150‑1 (MQ=255)

                                                                                                                                            ccGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaaa           <  1:1303030/150‑1 (MQ=255)

                                                                                                                                             cGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAAc          <  2:2422952/150‑1 (MQ=255)

                                                                                                                                               cAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaa        <  2:3880935/150‑1 (MQ=255)

                                                                                                                                                 gAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaaaa      >  1:3502660/1‑150 (MQ=255)

                                                                                                                                                     aaCGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCa  >  1:3952346/1‑150 (MQ=255)

AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`