Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,465,086 G→T 5.8% A136A (GCG→GCT)  tabA → biofilm modulator regulated by toxins; DUF386 family protein, cupin superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,465,0860GT 5.8% 1238.9 / 32.6 328A136A (GCG→GCT) tabAbiofilm modulator regulated by toxins; DUF386 family protein, cupin superfamily
Reads supporting (aligned to +/- strand):  ref base G (188/121);  new base T (9/10);  total (197/131)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 337 total aligned reads displayed.

CAGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTAT  >  CP009273/4464938‑4465235
                                                                                                                                                    |                                                                                                                                                     
 aGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGcc                                                                                                                                                     >  1:2527790/1‑150 (MQ=255)
 aGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGcc                                                                                                                                                     >  1:4237615/1‑150 (MQ=255)
      cgcCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGccc                                                                                                                                                >  2:4249414/1‑150 (MQ=255)
        ccGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAg                                                                                                                                              >  2:842491/1‑150 (MQ=255)
            aTACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTc                                                                                                                                          >  2:34016/1‑150 (MQ=255)
                cGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCaa                                                                                                                                      >  1:2892252/1‑150 (MQ=255)
                      gTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCagt                                                                                                                                <  2:300648/150‑1 (MQ=255)
                      gTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCagt                                                                                                                                <  2:3239029/150‑1 (MQ=255)
                           cTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGGGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGa                                                                                                                           >  2:671977/1‑150 (MQ=255)
                            tGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGaa                                                                                                                          >  2:1909016/1‑150 (MQ=255)
                                aaaGACATCGCATTTTTGCCGGACGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATg                                                                                                                      <  2:3315638/150‑1 (MQ=255)
                                aaaGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATg                                                                                                                      >  2:2301677/1‑150 (MQ=255)
                                  aGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCt                                                                                                                    <  1:2776889/150‑1 (MQ=255)
                                        cGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGc                                                                                                              <  2:599489/150‑1 (MQ=255)
                                           aTTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCGGTAGTGAAGATGCTGATGGCGtg                                                                                                           >  2:1782883/1‑150 (MQ=255)
                                           aTTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGtg                                                                                                           >  2:1027369/1‑150 (MQ=255)
                                           aTTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGtg                                                                                                           >  1:3172450/1‑150 (MQ=255)
                                           aTTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGtg                                                                                                           >  2:3836917/1‑150 (MQ=255)
                                                tGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGAc                                                                                                      <  1:4107677/150‑1 (MQ=255)
                                                 gCCGGAAGGCGTTGATGAGAAAACATTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACt                                                                                                     <  2:3506693/150‑1 (MQ=255)
                                                  ccGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACtt                                                                                                    >  2:362160/1‑150 (MQ=255)
                                                   cGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACttt                                                                                                   <  2:4164625/150‑1 (MQ=255)
                                                    ggAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACtttt                                                                                                  <  1:194032/150‑1 (MQ=255)
                                                    ggAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACtttt                                                                                                  <  2:4228178/150‑1 (MQ=255)
                                                     gAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTc                                                                                                 >  1:1562675/1‑150 (MQ=255)
                                                      aaGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGTAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCg                                                                                                <  1:3962545/150‑1 (MQ=255)
                                                      aaGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCg                                                                                                <  2:2134121/150‑1 (MQ=255)
                                                      aaGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCg                                                                                                >  1:421318/1‑150 (MQ=255)
                                                      aaGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCg                                                                                                >  1:352597/1‑150 (MQ=255)
                                                         gCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCg                                                                                             >  2:1682572/1‑150 (MQ=255)
                                                           gTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTa                                                                                           >  2:4061180/1‑150 (MQ=255)
                                                           gTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTa                                                                                           >  2:157604/1‑150 (MQ=255)
                                                           gTTGATGAGAAAACAGTTATTCTAAATGAAGGTGAGTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCTCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTa                                                                                           >  1:3105458/1‑150 (MQ=255)
                                                            ttGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTaa                                                                                          <  2:162531/150‑1 (MQ=255)
                                                             tgatgaGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTaaa                                                                                         <  2:2084383/150‑1 (MQ=255)
                                                             tgatgaGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTaaa                                                                                         >  2:3069343/1‑150 (MQ=255)
                                                                tgaGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATaa                                                                                      >  1:4297221/1‑150 (MQ=255)
                                                                tgaGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATaa                                                                                      >  2:277064/1‑150 (MQ=255)
                                                                  agaAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACt                                                                                    <  1:3017100/150‑1 (MQ=255)
                                                                   gtaaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTc                                                                                   <  1:3628949/148‑1 (MQ=255)
                                                                   gaAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCTCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTc                                                                                   <  1:4159692/150‑1 (MQ=255)
                                                                    aaaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCa                                                                                  <  1:2576398/150‑1 (MQ=255)
                                                                    aaaaCAGTTATTCTAAATGAAGGTGATTTGGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCAGATGGCGTGATGACTTTTCGCCGTAAATAACTCa                                                                                  <  1:4422917/150‑1 (MQ=255)
                                                                     aaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCaa                                                                                 <  2:2770272/150‑1 (MQ=255)
                                                                     aaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCaa                                                                                 >  2:3714032/1‑150 (MQ=255)
                                                                     aaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCaa                                                                                 >  1:2355864/1‑150 (MQ=255)
                                                                     aaaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCaa                                                                                 >  1:2871236/1‑150 (MQ=255)
                                                                     aaaCAGTTATTCTAAAAGAAGGTGATTTTGTTGTGTTTTAACCGGGGGAAGTGCATAAACCTCTTTGCGAAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTTATGACTTTTCGCCGTAAAAAACTCaa                                                                                 >  1:3669944/1‑150 (MQ=255)
                                                                      aaCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAg                                                                                >  1:798636/1‑150 (MQ=255)
                                                                       aCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAgg                                                                               >  2:3189881/1‑150 (MQ=255)
                                                                       aCAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAgg                                                                               >  1:2122800/1‑150 (MQ=255)
                                                                         aGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGtt                                                                             >  2:3277886/1‑150 (MQ=255)
                                                                         aGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGtt                                                                             >  2:2391000/1‑150 (MQ=255)
                                                                         aGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGtt                                                                             <  1:3568806/150‑1 (MQ=255)
                                                                          gTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCTCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGttt                                                                            <  1:2902930/150‑1 (MQ=255)
                                                                          gTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGttt                                                                            >  1:130828/1‑150 (MQ=255)
                                                                            tATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTAc                                                                          <  2:709551/150‑1 (MQ=255)
                                                                             aTTCTAAATGAAGGTTATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACg                                                                         >  2:754623/1‑150 (MQ=255)
                                                                                   aaTGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGt                                                                   >  1:1000888/1‑150 (MQ=255)
                                                                                     tGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGttt                                                                 <  1:1088852/150‑1 (MQ=255)
                                                                                     tGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGttt                                                                 >  1:3433699/1‑150 (MQ=255)
                                                                                      gAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTtg                                                                >  1:2254148/1‑150 (MQ=255)
                                                                                           tGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGaaa                                                           >  1:1743834/1‑150 (MQ=255)
                                                                                            gATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGaaaa                                                          >  2:2886520/1‑150 (MQ=255)
                                                                                             aTTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAag                                                         >  1:4062916/1‑150 (MQ=255)
                                                                                                      tgtTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGttttt                                                >  1:802324/1‑150 (MQ=255)
                                                                                                       gtTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGtttttt                                               <  2:4396404/150‑1 (MQ=255)
                                                                                                        ttttATCCGGGGGAAGTGCATAATCCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTg                                              <  1:1790822/150‑1 (MQ=255)
                                                                                                        ttttATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTg                                              >  1:4529543/1‑150 (MQ=255)
                                                                                                          ttATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGat                                            <  1:4333812/150‑1 (MQ=255)
                                                                                                           tATCCGGGGGAAGTGCATAAACCGCTGTGTGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGata                                           >  2:3125866/1‑150 (MQ=255)
                                                                                                           tATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGata                                           >  2:534036/1‑150 (MQ=255)
                                                                                                              ccGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATAttt                                        >  1:1403613/1‑150 (MQ=255)
                                                                                                                 ggggAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATAtttttt                                     <  1:1973068/150‑1 (MQ=255)
                                                                                                                       gTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAg                               >  1:72279/1‑150 (MQ=255)
                                                                                                                       gTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAg                               <  2:1336789/150‑1 (MQ=255)
                                                                                                                          cATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTaa                            >  1:1878815/1‑150 (MQ=255)
                                                                                                                          cATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTaa                            >  2:2952629/1‑150 (MQ=255)
                                                                                                                          cATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTaa                            <  2:3875954/150‑1 (MQ=255)
                                                                                                                            tAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTaaaa                          >  1:1335319/1‑150 (MQ=255)
                                                                                                                            tAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTaaaa                          >  1:1335330/1‑150 (MQ=255)
                                                                                                                             aaaCCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAAt                         >  2:1214875/1‑150 (MQ=255)
                                                                                                                             aaaCCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAAt                         >  2:458518/1‑150 (MQ=255)
                                                                                                                             aaaCCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAAt                         >  2:203977/1‑150 (MQ=255)
                                                                                                                                   cTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTaa                   >  2:1473089/1‑150 (MQ=255)
                                                                                                                                    tgtgCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAAt                  >  1:2686261/1‑150 (MQ=255)
                                                                                                                                     gtgCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATg                 <  2:925666/150‑1 (MQ=255)
                                                                                                                                     gtgCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATg                 <  2:1069710/150‑1 (MQ=255)
                                                                                                                                      tgCGCAGTGGGCGCTCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGc                >  1:1501167/1‑150 (MQ=255)
                                                                                                                                      tgCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGc                >  2:3307406/1‑150 (MQ=255)
                                                                                                                                      tgCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGc                <  1:1269180/150‑1 (MQ=255)
                                                                                                                                          cAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAg            <  1:2754646/150‑1 (MQ=255)
                                                                                                                                          cAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAg            <  1:4058937/150‑1 (MQ=255)
                                                                                                                                            gTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGAc          <  1:2138438/150‑1 (MQ=255)
                                                                                                                                            gTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGAc          >  1:960022/1‑150 (MQ=255)
                                                                                                                                              gggCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGt        >  1:3789664/1‑150 (MQ=255)
                                                                                                                                              gggCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAAATAGTAATGCTTAGACGt        >  2:252419/1‑150 (MQ=255)
                                                                                                                                               ggCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGtt       <  1:223969/150‑1 (MQ=255)
                                                                                                                                                  gcgcCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCtt    <  1:362160/150‑1 (MQ=255)
                                                                                                                                                  gcgcCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCtt    >  2:2680259/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTAT  >  CP009273/4464938‑4465235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑