BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,465,624	C→T	10.6%	S124F (TCC→TTC)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,465,624	0	C	T	10.6%	1006.0 / 82.5	322	S124F (TCC→TTC)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (155/133); new base T (20/14); total (175/147)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 327 total aligned reads displayed.

CAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGT  >  CP009273/4465475‑4465772

cAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTc                                                                                                                                                      <  1:1084688/150‑1 (MQ=255)

 aaCGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTcc                                                                                                                                                     >  2:594783/1‑150 (MQ=255)

  aCGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCa                                                                                                                                                    <  2:3100296/150‑1 (MQ=255)

   cGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCAc                                                                                                                                                   <  1:3028805/150‑1 (MQ=255)

     aaGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTa                                                                                                                                                 >  2:4537170/1‑150 (MQ=255)

       gCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATc                                                                                                                                               <  1:767327/150‑1 (MQ=255)

       gCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATc                                                                                                                                               <  1:2354527/150‑1 (MQ=255)

        cGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCa                                                                                                                                              <  2:1650053/150‑1 (MQ=255)

           gTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTTCATGAATAATCCTGTAGGGGAACGAGCGGATTCACACAAGGTGATTATTGAAATTTCACTGTCAACTATCacta                                                                                                                                           >  2:3677846/1‑150 (MQ=255)

               aaaTATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTAt                                                                                                                                       >  1:2890016/1‑150 (MQ=255)

               aaaTATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTAt                                                                                                                                       <  2:2705602/150‑1 (MQ=255)

               aaaTATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTAt                                                                                                                                       <  2:4039321/150‑1 (MQ=255)

                aaTATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATg                                                                                                                                      >  1:329577/1‑150 (MQ=255)

                 atatGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATgg                                                                                                                                     >  2:861289/1‑150 (MQ=255)

                 atatGATGGCTCATAATATGTACTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATgg                                                                                                                                     <  2:252344/150‑1 (MQ=255)

                    tgatgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGa                                                                                                                                  >  2:4473399/1‑150 (MQ=255)

                     gatgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGAc                                                                                                                                 >  2:809670/1‑150 (MQ=255)

                      atgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACt                                                                                                                                >  2:3793437/1‑150 (MQ=255)

                      atgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACt                                                                                                                                <  2:690552/150‑1 (MQ=255)

                            cATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCaa                                                                                                                          >  2:3498651/1‑150 (MQ=255)

                             atatTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAg                                                                                                                         >  2:4128356/1‑150 (MQ=255)

                             atatTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAg                                                                                                                         >  1:1035582/1‑150 (MQ=255)

                             atatTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCAGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAg                                                                                                                         <  1:2240269/150‑1 (MQ=255)

                                 tAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACa                                                                                                                     >  1:3310454/1‑150 (MQ=255)

                                 tAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACa                                                                                                                     >  1:2636879/1‑150 (MQ=255)

                                 tAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACa                                                                                                                     >  1:1032474/1‑150 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACAt                                                                                                                    >  1:639034/1‑150 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTc                                                                                                                            <  2:1049676/142‑1 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACAt                                                                                                                    >  2:273094/1‑150 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACAt                                                                                                                    >  1:686661/1‑150 (MQ=255)

                                   aGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATa                                                                                                                   >  2:2041968/1‑150 (MQ=255)

                                   aGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATa                                                                                                                   <  1:1636243/150‑1 (MQ=255)

                                     tCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACa                                                                                                                 <  1:4252053/150‑1 (MQ=255)

                                      ccTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAg                                                                                                                >  1:1531179/1‑150 (MQ=255)

                                       cTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGc                                                                                                               <  1:1717441/150‑1 (MQ=255)

                                       cTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGc                                                                                                               <  1:366209/150‑1 (MQ=255)

                                         cATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCa                                                                                                             >  1:4055229/1‑150 (MQ=255)

                                          aTCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCAt                                                                                                            <  2:3571389/150‑1 (MQ=255)

                                            cTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATta                                                                                                          >  2:3326125/1‑150 (MQ=255)

                                              tATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATtata                                                                                                        <  2:1259306/150‑1 (MQ=255)

                                               aTTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTatat                                                                                                       <  1:2881117/150‑1 (MQ=255)

                                                   tAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTcc                                                                                                   <  1:2412629/150‑1 (MQ=255)

                                                   tAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTcc                                                                                                   <  1:4246621/150‑1 (MQ=255)

                                                      aGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCcaca                                                                                                >  1:4102536/1‑150 (MQ=255)

                                                         tACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAAcc                                                                                             <  1:3349796/150‑1 (MQ=255)

                                                         tACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAAcc                                                                                             <  2:3895915/150‑1 (MQ=255)

                                                          tcTAATTGCAATGAAAAAATGATGATTTACATGAATAATCCTGTTGGTGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTCTGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACATCCt                                                                                            <  1:712414/149‑1 (MQ=255)

                                                           cTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTg                                                                                           <  1:372675/150‑1 (MQ=255)

                                                           cTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTg                                                                                           >  1:3373510/1‑150 (MQ=255)

                                                              aTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATg                                                                                        <  2:1006480/150‑1 (MQ=255)

                                                                ggCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGt                                                                                      >  1:13885/1‑150 (MQ=255)

                                                                ggCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGt                                                                                      >  1:13871/1‑150 (MQ=255)

                                                                ggCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGt                                                                                      >  1:2739068/1‑150 (MQ=255)

                                                                             tGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGtt                                                                         <  1:2019201/150‑1 (MQ=255)

                                                                                 gTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGa                                                                     <  1:2714054/150‑1 (MQ=255)

                                                                                   tttACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAg                                                                   <  1:3398761/150‑1 (MQ=255)

                                                                                   tttACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAg                                                                   <  1:4537402/150‑1 (MQ=255)

                                                                                    ttACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAgg                                                                  >  1:1310903/1‑150 (MQ=255)

                                                                                      aCATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGa                                                                >  2:4311147/1‑150 (MQ=255)

                                                                                       cATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGaa                                                               <  2:3241222/150‑1 (MQ=255)

                                                                                        aTGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAg                                                              >  1:446873/1‑150 (MQ=255)

                                                                                              aatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCttt                                                        >  1:620325/1‑150 (MQ=255)

                                                                                              aatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCttt                                                        >  2:3364794/1‑150 (MQ=255)

                                                                                              aatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTGTCCTCTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCTCAACGTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCttt                                                        <  2:4409466/150‑1 (MQ=255)

                                                                                                 ccTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaa                                                     <  1:273094/150‑1 (MQ=255)

                                                                                                  cTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaa                                                    >  1:4508584/1‑150 (MQ=255)

                                                                                                   tGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaag                                                   <  1:2550942/150‑1 (MQ=255)

                                                                                                     tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaagaa                                                 <  2:1941293/150‑1 (MQ=255)

                                                                                                      aGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaagaaa                                                <  1:213680/150‑1 (MQ=255)

                                                                                                      aGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCAACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaagaaa                                                >  1:1942438/1‑150 (MQ=255)

                                                                                                       ggggAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGaaagaaaa                                               <  1:1235982/150‑1 (MQ=255)

                                                                                                          gAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGAt                                            <  1:2772862/150‑1 (MQ=255)

                                                                                                           aaCGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATg                                           <  2:1701968/150‑1 (MQ=255)

                                                                                                              gAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATgaga                                        >  2:4295441/1‑150 (MQ=255)

                                                                                                               aGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGagag                                       >  2:4267720/1‑150 (MQ=255)

                                                                                                               aGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATCTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGTAAAGATGagag                                       >  1:2771448/1‑150 (MQ=255)

                                                                                                                 cGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGtt                                     <  1:3126530/150‑1 (MQ=255)

                                                                                                                 cGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGtt                                     >  2:1081044/1‑150 (MQ=255)

                                                                                                                 cGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGtt                                     >  2:1546979/1‑150 (MQ=255)

                                                                                                                  ggATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTc                                    <  2:3734248/150‑1 (MQ=255)

                                                                                                                  ggATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTc                                    >  1:3810750/1‑150 (MQ=255)

                                                                                                                   gATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCa                                   <  1:2292535/150‑1 (MQ=255)

                                                                                                                          ccAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACAcc                            <  2:162586/150‑1 (MQ=255)

                                                                                                                             aGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGa                         >  2:1325230/1‑150 (MQ=255)

                                                                                                                              ggTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGAt                        <  1:594783/150‑1 (MQ=255)

                                                                                                                               gTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGAtt                       >  1:3555726/1‑150 (MQ=255)

                                                                                                                                     ataGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAAc                 >  2:2601567/1‑150 (MQ=255)

                                                                                                                                      taGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACa                <  1:3978834/150‑1 (MQ=255)

                                                                                                                                      taGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACa                <  1:1824618/150‑1 (MQ=255)

                                                                                                                                       aGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTCCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACaa               >  1:285883/1‑150 (MQ=255)

                                                                                                                                        gAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAg              >  2:2431919/1‑150 (MQ=255)

                                                                                                                                            tttcacttttcactATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTc          >  2:3385492/1‑150 (MQ=255)

                                                                                                                                            tttCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTc          <  2:3801071/150‑1 (MQ=255)

                                                                                                                                            tttCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTc          >  2:1162261/1‑150 (MQ=255)

                                                                                                                                             ttCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCt         >  1:1286527/1‑150 (MQ=255)

                                                                                                                                              tCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTg        <  1:1772727/150‑1 (MQ=255)

                                                                                                                                               cACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTgg       >  2:2985785/1‑150 (MQ=255)

                                                                                                                                                   ttCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg   >  2:2324286/1‑150 (MQ=255)

                                                                                                                                                   ttCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg   >  2:2162189/1‑150 (MQ=255)

                                                                                                                                                    tCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtgt  >  2:1873858/1‑150 (MQ=255)

CAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGT  >  CP009273/4465475‑4465772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`