BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,465,818	A→C	6.4%	N189H (AAT→CAT)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,465,818	0	A	C	6.4%	975.4 / 51.4	324	N189H (AAT→CAT)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (132/171); new base C (15/6); total (147/177)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

TATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAG  >  CP009273/4465669‑4465967

tatTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAaataata                                                                                                                                                       >  2:2557735/1‑150 (MQ=255)

 atTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAaataataa                                                                                                                                                      <  2:4264697/150‑1 (MQ=255)

  tttCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAaataataat                                                                                                                                                     >  1:3491997/1‑150 (MQ=255)

     ccACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAg                                                                                                                                                  <  1:1024129/150‑1 (MQ=255)

       acaACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGa                                                                                                                                                >  1:1853158/1‑150 (MQ=255)

         aaCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGt                                                                                                                                              >  2:1258306/1‑150 (MQ=255)

          aCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAACGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGtt                                                                                                                                             <  2:3671247/150‑1 (MQ=255)

          aCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGtt                                                                                                                                             >  2:3757026/1‑150 (MQ=255)

          aCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGtt                                                                                                                                             <  2:1088501/150‑1 (MQ=255)

          aCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGtt                                                                                                                                             <  2:2963366/150‑1 (MQ=255)

          aCCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGtt                                                                                                                                             >  1:1658270/1‑150 (MQ=255)

             tGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTaaa                                                                                                                                          >  1:902706/1‑150 (MQ=255)

               aTGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAaata                                                                                                                                        >  2:3827905/1‑150 (MQ=255)

               aTGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAaata                                                                                                                                        <  1:157941/150‑1 (MQ=255)

               aTGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAaata                                                                                                                                        <  1:3326986/150‑1 (MQ=255)

                  gTTCGACTAACCGTTATGAAGGGACGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATCATAATCAGGAGTTAAATAATc                                                                                                                                     >  2:3975331/1‑150 (MQ=255)

                    tCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTa                                                                                                                                   <  1:3740774/150‑1 (MQ=255)

                     cGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTAc                                                                                                                                  >  1:3817011/1‑150 (MQ=255)

                     cGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTAc                                                                                                                                  >  1:3397790/1‑150 (MQ=255)

                        cTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGa                                                                                                                               >  2:1639897/1‑150 (MQ=255)

                         tAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGaa                                                                                                                              <  2:1033208/150‑1 (MQ=255)

                           aCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAt                                                                                                                            <  1:2324286/150‑1 (MQ=255)

                           aCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAt                                                                                                                            <  1:3793437/150‑1 (MQ=255)

                           aCCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAt                                                                                                                            <  2:620325/150‑1 (MQ=255)

                            ccGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAAtt                                                                                                                           >  2:3012664/1‑150 (MQ=255)

                             cGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTa                                                                                                                          <  2:14087/150‑1 (MQ=255)

                             cGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTa                                                                                                                          <  2:13877/150‑1 (MQ=255)

                               ttATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTaaa                                                                                                                        <  2:3484200/150‑1 (MQ=255)

                                   gAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaataat                                                                                                                    >  1:2655429/1‑150 (MQ=255)

                                    aaGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTACAGAAATTAAATAATc                                                                                                                   <  1:3364794/150‑1 (MQ=255)

                                    aaGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATc                                                                                                                   <  1:3050075/150‑1 (MQ=255)

                                    aaGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAATTAAATAATCTACAGAAATTAAATAATc                                                                                                                   >  2:326823/1‑150 (MQ=255)

                                     aGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCt                                                                                                                  <  2:593525/150‑1 (MQ=255)

                                     aGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCt                                                                                                                  >  2:2698324/1‑150 (MQ=255)

                                           gTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGaa                                                                                                            <  1:4486757/150‑1 (MQ=255)

                                              cTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGtt                                                                                                         >  1:4016161/1‑150 (MQ=255)

                                                ttGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTaa                                                                                                       >  2:3568171/1‑150 (MQ=255)

                                                  gaaagaaaAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaat                                                                                                     <  2:2853687/150‑1 (MQ=255)

                                                    aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                   <  1:1162261/150‑1 (MQ=255)

                                                      gaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATc                                                                                                 <  1:4223202/150‑1 (MQ=255)

                                                       aaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCt                                                                                                <  1:1325230/150‑1 (MQ=255)

                                                       aaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAATTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCt                                                                                                <  1:326823/150‑1 (MQ=255)

                                                        aaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTa                                                                                               <  2:4102536/150‑1 (MQ=255)

                                                        aaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTa                                                                                               >  2:131677/1‑150 (MQ=255)

                                                        aaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTa                                                                                               >  2:2431939/1‑150 (MQ=255)

                                                          agaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACt                                                                                             >  1:1907311/1‑150 (MQ=255)

                                                           gaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTg                                                                                            <  2:3169007/150‑1 (MQ=255)

                                                           gaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTg                                                                                            <  2:2771484/150‑1 (MQ=255)

                                                              gagaGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAg                                                                                         <  2:668828/150‑1 (MQ=255)

                                                              gagaGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAg                                                                                         >  2:148408/1‑150 (MQ=255)

                                                               agagTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGt                                                                                        <  1:745136/150‑1 (MQ=255)

                                                                  gTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaa                                                                                     >  1:2065003/1‑150 (MQ=255)

                                                                  gTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaa                                                                                     >  2:3580058/1‑150 (MQ=255)

                                                                     cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                  >  1:145841/1‑150 (MQ=255)

                                                                     cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                  >  2:2353936/1‑150 (MQ=255)

                                                                     cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                  <  1:127117/150‑1 (MQ=255)

                                                                     cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                  <  2:2736884/150‑1 (MQ=255)

                                                                       ttACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataat                                                                                >  2:1443307/1‑150 (MQ=255)

                                                                             cTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAg                                                                          <  2:1340152/150‑1 (MQ=255)

                                                                               gATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAggg                                                                        >  2:91318/1‑150 (MQ=255)

                                                                                aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAgggg                                                                       >  1:385958/1‑150 (MQ=255)

                                                                                aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAgggg                                                                       >  1:386565/1‑150 (MQ=255)

                                                                                aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAgggg                                                                       <  1:2442357/150‑1 (MQ=255)

                                                                                aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAgggg                                                                       >  1:4119746/1‑150 (MQ=255)

                                                                                 ttACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGt                                                                      <  1:3523668/150‑1 (MQ=255)

                                                                                  tACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGtt                                                                     <  1:1390763/150‑1 (MQ=255)

                                                                                   aCTAACAATTTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTa                                                                    <  2:2739149/150‑1 (MQ=255)

                                                                                    cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaa                                                                   <  2:1286619/150‑1 (MQ=255)

                                                                                         aaGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaataat                                                              <  1:4311147/150‑1 (MQ=255)

                                                                                              tCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                         >  2:1339815/1‑150 (MQ=255)

                                                                                              tCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                         <  1:1081044/150‑1 (MQ=255)

                                                                                              tCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                         <  1:1546983/150‑1 (MQ=255)

                                                                                                tGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAgg                                                       >  1:768847/1‑150 (MQ=255)

                                                                                                 ggCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGa                                                      <  2:4508433/150‑1 (MQ=255)

                                                                                                    gtgtTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGtt                                                   >  1:1235156/1‑150 (MQ=255)

                                                                                                       ttACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTaaa                                                >  1:529120/1‑150 (MQ=255)

                                                                                                        tACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaat                                               <  1:1483153/150‑1 (MQ=255)

                                                                                                             aaGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATcc                                          <  2:865490/150‑1 (MQ=255)

                                                                                                              aGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCg                                         >  1:769413/1‑150 (MQ=255)

                                                                                                                tGAAGCTAACAAGAAAATAGCGCTTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCa                                       <  2:1848032/150‑1 (MQ=255)

                                                                                                                 gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAg                                      >  2:4486649/1‑150 (MQ=255)

                                                                                                                 gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCATCAGGAGTTAAATAATCCGCAg                                      >  2:4487207/1‑150 (MQ=255)

                                                                                                                  aaGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAATTAATCTACTGAAGTTCAATAATATACAGGGGTTAAATAATCCTCCGGAGTTAAATAATCCGCAGa                                     >  2:4244584/1‑150 (MQ=255)

                                                                                                                      tAACAATAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATCATCCTCAGGAGTTAAATAATCCGCAGAAttt                                 <  1:3684758/150‑1 (MQ=255)

                                                                                                                      tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                 >  1:2267818/1‑150 (MQ=255)

                                                                                                                             aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                          >  1:3865470/1‑150 (MQ=255)

                                                                                                                             aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                          <  2:1853158/150‑1 (MQ=255)

                                                                                                                             aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                          >  2:640234/1‑150 (MQ=255)

                                                                                                                             aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAt                          <  1:3596163/150‑1 (MQ=255)

                                                                                                                               aaTAGCGCGTCTATTAAATAATCCTCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtc                        <  1:2985797/150‑1 (MQ=255)

                                                                                                                                 tAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtctc                      <  1:2698324/150‑1 (MQ=255)

                                                                                                                                         cTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTa              >  2:3474048/1‑150 (MQ=255)

                                                                                                                                           aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa            <  1:827469/150‑1 (MQ=255)

                                                                                                                                             tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATa          >  2:1554826/1‑150 (MQ=255)

                                                                                                                                             tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATa          <  2:4119795/150‑1 (MQ=255)

                                                                                                                                             tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATa          >  2:2259668/1‑150 (MQ=255)

                                                                                                                                             tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATa          <  1:4267720/150‑1 (MQ=255)

                                                                                                                                                 taataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTc      <  1:2952311/150‑1 (MQ=255)

                                                                                                                                                     aatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAg  >  2:430075/1‑150 (MQ=255)

                                                                                                                                                     aatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAg  >  2:2620009/1‑150 (MQ=255)

TATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAG  >  CP009273/4465669‑4465967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`