Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,494,150 C→A 5.4% V92V (GTC→GTA)  yjhB → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,494,1500CA 5.4% 1200.2 / 27.3 316V92V (GTC→GTA) yjhBputative transporter
Reads supporting (aligned to +/- strand):  ref base C (138/161);  new base A (10/7);  total (148/168)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 321 total aligned reads displayed.

GCAGATCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAGCTAGTG  >  CP009273/4494001‑4494298
                                                                                                                                                     |                                                                                                                                                    
gCAGATCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTc                                                                                                                                                      <  2:3255996/150‑1 (MQ=255)
 cAGATCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCg                                                                                                                                                     <  2:4254924/150‑1 (MQ=255)
 cAGATCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCg                                                                                                                                                     >  1:1894993/1‑150 (MQ=255)
    aTCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGaa                                                                                                                                                  >  1:3731856/1‑150 (MQ=255)
     tCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAAc                                                                                                                                                 <  1:2466044/150‑1 (MQ=255)
     tCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAAc                                                                                                                                                 >  1:1805828/1‑150 (MQ=255)
      cTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACa                                                                                                                                                >  2:2140839/1‑150 (MQ=255)
         ggCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGc                                                                                                                                             >  1:721135/1‑150 (MQ=255)
            aTTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCtt                                                                                                                                          <  2:1947450/150‑1 (MQ=255)
             ttACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTa                                                                                                                                         >  1:135445/1‑150 (MQ=255)
                cGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCg                                                                                                                                      <  1:3397405/150‑1 (MQ=255)
                 ggATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCgg                                                                                                                                     >  1:2038540/1‑150 (MQ=255)
                   atatTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTa                                                                                                                                   >  1:4268596/1‑150 (MQ=255)
                   atatTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTa                                                                                                                                   >  1:3014858/1‑150 (MQ=255)
                    tatTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTAt                                                                                                                                  >  2:450944/1‑150 (MQ=255)
                     atTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTAtt                                                                                                                                 >  2:3755438/1‑150 (MQ=255)
                     atTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTAtt                                                                                                                                 <  1:2687735/150‑1 (MQ=255)
                       tCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGc                                                                                                                               <  2:4374498/150‑1 (MQ=255)
                       tCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGc                                                                                                                               >  2:2884718/1‑150 (MQ=255)
                        cAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCt                                                                                                                              >  1:3761004/1‑150 (MQ=255)
                         aGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTa                                                                                                                             >  2:364662/1‑150 (MQ=255)
                         aGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTa                                                                                                                             <  1:4217197/150‑1 (MQ=255)
                         aGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTa                                                                                                                             <  2:416671/150‑1 (MQ=255)
                           gCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACa                                                                                                                           >  1:3641159/1‑150 (MQ=255)
                           gCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACa                                                                                                                           >  1:326534/1‑150 (MQ=255)
                            cTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACaa                                                                                                                          <  1:3069415/150‑1 (MQ=255)
                             ttcTTTAATAGGGACAGTGGCATTCATAGACAGACCTATTGGAGGTGGTTTTTTTGTTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCATTCGGAACAGGCATTAGCGGTATTGCTACaaa                                                                                                                         <  2:2719445/148‑1 (MQ=255)
                             tACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACaaa                                                                                                                         <  1:2786655/150‑1 (MQ=255)
                              aCTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTATTGCTACAAAc                                                                                                                        >  2:4259254/1‑150 (MQ=255)
                                 ttAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTta                                                                                                                     <  1:1174048/150‑1 (MQ=255)
                                 ttAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTATTGCTACAAACTta                                                                                                                     <  1:4454974/150‑1 (MQ=255)
                                  tAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTtat                                                                                                                    >  2:2269309/1‑150 (MQ=255)
                                   aaTAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTtata                                                                                                                   <  2:1527251/150‑1 (MQ=255)
                                      aGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTatatat                                                                                                                <  1:3758347/150‑1 (MQ=255)
                                       gggACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATg                                                                                                               >  2:4142624/1‑150 (MQ=255)
                                        ggACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGT‑GGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCt                                                                                                             <  2:1419567/150‑1 (MQ=255)
                                           cAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCg                                                                                                           >  2:3834000/1‑150 (MQ=255)
                                            aGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGc                                                                                                          <  1:2856565/150‑1 (MQ=255)
                                            aGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGc                                                                                                          >  2:3477569/1‑150 (MQ=255)
                                              tGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAg                                                                                                        <  2:2051141/150‑1 (MQ=255)
                                               ggCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGt                                                                                                       >  2:1869820/1‑150 (MQ=255)
                                               ggCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGt                                                                                                       <  2:4111835/150‑1 (MQ=255)
                                                gCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGtt                                                                                                      <  1:271407/150‑1 (MQ=255)
                                                  cTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCAATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTg                                                                                                    >  2:3768357/1‑150 (MQ=255)
                                                   ttCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGT‑GGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGcc                                                                                                  <  1:21315/150‑1 (MQ=255)
                                                      aTAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGt                                                                                                >  2:1620618/1‑150 (MQ=255)
                                                      aTAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGT‑GGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGtt                                                                                               >  1:1824120/1‑150 (MQ=255)
                                                            aGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTAtt                                                                                          >  2:1883293/1‑150 (MQ=255)
                                                                 tATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTgg                                                                                     <  1:3469677/150‑1 (MQ=255)
                                                                  aTTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGc                                                                                    >  2:2577093/1‑150 (MQ=255)
                                                                  aTTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGc                                                                                    <  2:606570/150‑1 (MQ=255)
                                                                  aTTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGc                                                                                    <  2:1169834/150‑1 (MQ=255)
                                                                    tGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCtt                                                                                  <  1:4252856/150‑1 (MQ=255)
                                                                     ggAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTa                                                                                 <  2:1452034/150‑1 (MQ=255)
                                                                       aGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAgg                                                                               <  1:998074/150‑1 (MQ=255)
                                                                          tggtTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGAt                                                                            <  1:2954121/150‑1 (MQ=255)
                                                                           ggtTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATg                                                                           <  1:1241158/150‑1 (MQ=255)
                                                                            gtTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGt                                                                          <  2:135445/150‑1 (MQ=255)
                                                                               tttttGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTg                                                                       >  1:3222163/1‑150 (MQ=255)
                                                                               tttttGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTAGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTg                                                                       >  1:4012186/1‑150 (MQ=255)
                                                                                  ttGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTg                                                                    >  2:3774642/1‑150 (MQ=255)
                                                                                  ttGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTg                                                                    >  2:2412514/1‑150 (MQ=255)
                                                                                     gTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAat                                                                 <  1:1502947/150‑1 (MQ=255)
                                                                                       gCCATGGCTGATAAATATGGTCGTAAGCAAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCTGTATTGCTACAAACTTTTATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAatat                                                               <  2:3458391/150‑1 (MQ=255)
                                                                                         cATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGc                                                             <  1:48224/150‑1 (MQ=255)
                                                                                             gCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCAtgt                                                         <  1:2345555/150‑1 (MQ=255)
                                                                                               tGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGc                                                       >  1:1087674/1‑150 (MQ=255)
                                                                                                     atatGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAAc                                                 <  2:3989179/150‑1 (MQ=255)
                                                                                                        tGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTa                                              <  1:432628/150‑1 (MQ=255)
                                                                                                        tGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTa                                              <  1:2643604/150‑1 (MQ=255)
                                                                                                        tGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTa                                              <  1:2897434/150‑1 (MQ=255)
                                                                                                            cGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCg                                          <  2:1832101/150‑1 (MQ=255)
                                                                                                             gTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCgg                                         <  1:660744/150‑1 (MQ=255)
                                                                                                               aaGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTa                                       <  2:2170707/150‑1 (MQ=255)
                                                                                                               aaGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTa                                       >  2:532565/1‑150 (MQ=255)
                                                                                                                 gCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGa                                     <  2:852863/150‑1 (MQ=255)
                                                                                                                 gCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGa                                     <  2:4268596/150‑1 (MQ=255)
                                                                                                                 gCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGa                                     <  2:2019230/150‑1 (MQ=255)
                                                                                                                   cAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCAGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGaaa                                   >  1:1528939/1‑150 (MQ=255)
                                                                                                                       gatgatgTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTg                               <  2:3255125/150‑1 (MQ=255)
                                                                                                                        atgatgTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTgg                              >  2:1037190/1‑150 (MQ=255)
                                                                                                                        atgatgTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTgg                              >  1:606151/1‑150 (MQ=255)
                                                                                                                         tgatgTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGc                             >  1:2251364/1‑150 (MQ=255)
                                                                                                                           atgTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCt                           <  2:3163109/150‑1 (MQ=255)
                                                                                                                               gggCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGGTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTaaaa                       >  2:3553744/1‑150 (MQ=255)
                                                                                                                                 gaaaTTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAAt                     <  2:4465344/148‑1 (MQ=255)
                                                                                                                                  cAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATc                    <  1:1200335/150‑1 (MQ=255)
                                                                                                                                   aaTTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCt                   >  1:3543085/1‑150 (MQ=255)
                                                                                                                                    aTTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCtt                  >  2:571207/1‑150 (MQ=255)
                                                                                                                                    aTTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCtt                  <  1:4539087/150‑1 (MQ=255)
                                                                                                                                         cATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATc             >  1:1004769/1‑150 (MQ=255)
                                                                                                                                          aTTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCt            <  2:2788972/150‑1 (MQ=255)
                                                                                                                                            ttACTCAGTCGGAACAGGCCGTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTaa          <  2:1684116/150‑1 (MQ=255)
                                                                                                                                             tACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTaaa         <  1:3760218/150‑1 (MQ=255)
                                                                                                                                             tACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTaaa         >  1:883575/1‑150 (MQ=255)
                                                                                                                                              aCTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAg        <  1:2650132/150‑1 (MQ=255)
                                                                                                                                              aCTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAg        <  1:154168/150‑1 (MQ=255)
                                                                                                                                               cTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAGc       >  2:1159199/1‑150 (MQ=255)
                                                                                                                                                  aGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAGCTAg    <  1:3477569/150‑1 (MQ=255)
                                                                                                                                                    tCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAGCTAGTg  >  1:1965252/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAGATCTTGGCATTACGGATATTCAGGCTACTTTAATAGGGACAGTGGCCTTCATAGCCAGACCTATTGGAGGTGGTTTTTTTGGTGCCATGGCTGATAAATATGGTCGTAAGCCAATGATGATGTGGGCAATTTTCATTTACTCAGTCGGAACAGGCCTTAGCGGTATTGCTACAAACTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTGGTGAATATGCATGTGCTTCAACTTATGCGGTAGAAAGTTGGCCTAAAAATCTTCAATCTAAAGCTAGTG  >  CP009273/4494001‑4494298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑