BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,494,721	G→A	70.8%	A283T (GCC→ACC)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,494,721	0	G	A	70.8%	497.3 / 271.2	310	A283T (GCC→ACC)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base G (46/44); new base A (95/124); total (142/168)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 311 total aligned reads displayed.

CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATAT  >  CP009273/4494574‑4494868

ccGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAAcc                                                                                                                                                   >  1:1507397/1‑150 (MQ=255)

  gATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCtt                                                                                                                                                 >  1:3327212/1‑150 (MQ=255)

    tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTg                                                                                                                                               <  1:2537492/150‑1 (MQ=255)

    tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTg                                                                                                                                               <  2:1778736/150‑1 (MQ=255)

      aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTa                                                                                                                                             >  1:354621/1‑150 (MQ=255)

        cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGt                                                                                                                                           >  2:148960/1‑150 (MQ=255)

        cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGt                                                                                                                                           <  2:2025407/150‑1 (MQ=255)

         ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGTTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTc                                                                                                                                          >  2:3251000/1‑150 (MQ=255)

         ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                          >  2:812962/1‑150 (MQ=255)

         ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                          >  2:812803/1‑150 (MQ=255)

         ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTc                                                                                                                                          >  1:3910432/1‑150 (MQ=255)

           actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCgg                                                                                                                                        >  1:270807/1‑150 (MQ=255)

            ctactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGt                                                                                                                                       <  1:2966365/150‑1 (MQ=255)

            ctactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGt                                                                                                                                       >  2:1735074/1‑150 (MQ=255)

             tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTc                                                                                                                                      <  2:1062196/150‑1 (MQ=255)

              actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCt                                                                                                                                     >  2:2491030/1‑150 (MQ=255)

              actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCt                                                                                                                                     >  1:3771089/1‑150 (MQ=255)

               cttccttcCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCta                                                                                                                                    <  1:3677145/150‑1 (MQ=255)

                 tccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaat                                                                                                                                  <  2:1012261/150‑1 (MQ=255)

                      cctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACtt                                                                                                                             <  1:3187653/150‑1 (MQ=255)

                       ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTc                                                                                                                            <  1:2359762/150‑1 (MQ=255)

                        tACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCa                                                                                                                           <  1:1551840/150‑1 (MQ=255)

                          ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                         <  1:2929919/150‑1 (MQ=255)

                          ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                         >  1:1466910/1‑150 (MQ=255)

                          ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                         >  2:4199586/1‑150 (MQ=255)

                           cTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattt                                                                                                                        <  1:834486/150‑1 (MQ=255)

                            tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCattta                                                                                                                       <  1:691839/150‑1 (MQ=255)

                                     aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttt                                                                                                              <  2:2289590/150‑1 (MQ=255)

                                        gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAATCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                                                                                           >  1:169942/1‑150 (MQ=255)

                                           ttAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctc                                                                                                        >  1:2468162/1‑150 (MQ=255)

                                             aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                      <  1:1965729/150‑1 (MQ=255)

                                             aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                      >  1:968877/1‑150 (MQ=255)

                                              aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                     <  1:286917/150‑1 (MQ=255)

                                               tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttt                                                                                                    >  1:3519853/1‑150 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttt                                                                                                   <  2:1701975/150‑1 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttt                                                                                                   <  1:973998/150‑1 (MQ=255)

                                                 cAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttt                                                                                                  <  2:3913228/150‑1 (MQ=255)

                                                 cAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttt                                                                                                  <  1:1348537/150‑1 (MQ=255)

                                                   gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCGTTGTCCTCtttttttt                                                                                                >  1:2804359/1‑150 (MQ=255)

                                                    tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTa                                                                                               <  1:453916/150‑1 (MQ=255)

                                                     ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                              <  2:4050781/150‑1 (MQ=255)

                                                        cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                           <  2:1140863/150‑1 (MQ=255)

                                                        cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                           <  1:1587403/150‑1 (MQ=255)

                                                         aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                          >  2:3630781/1‑150 (MQ=255)

                                                         aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                          >  2:323234/1‑150 (MQ=255)

                                                            tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATTTTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTTTAGTCGGTCTAATATATTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgtg                                                                                       <  1:2573862/150‑1 (MQ=255)

                                                            tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgtg                                                                                       >  2:3574829/1‑150 (MQ=255)

                                                            tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgtg                                                                                       >  2:1304037/1‑150 (MQ=255)

                                                             cAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCATTTGTCCTCTTTTTTTTATTTCTGTGa                                                                                      >  2:1931255/1‑150 (MQ=255)

                                                              aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                                                                                     <  2:3161189/150‑1 (MQ=255)

                                                                   tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                                <  2:4541523/150‑1 (MQ=255)

                                                                    gatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActc                                                                               >  2:2532936/1‑150 (MQ=255)

                                                                     atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                              <  2:717005/150‑1 (MQ=255)

                                                                     atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                              <  2:1939093/150‑1 (MQ=255)

                                                                       gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                            >  1:1043404/1‑150 (MQ=255)

                                                                       gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                            <  2:2643334/150‑1 (MQ=255)

                                                                        aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttct                                                                           <  1:3429772/150‑1 (MQ=255)

                                                                              gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                     <  2:2348303/150‑1 (MQ=255)

                                                                                aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                   >  2:297719/1‑150 (MQ=255)

                                                                                aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                   <  2:756920/150‑1 (MQ=255)

                                                                                aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                   >  1:3821576/1‑150 (MQ=255)

                                                                                aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                   >  1:3821809/1‑150 (MQ=255)

                                                                                  gTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAt                                                                 <  2:4029193/150‑1 (MQ=255)

                                                                                     tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                              <  2:3213510/150‑1 (MQ=255)

                                                                                     tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                              <  2:4163224/150‑1 (MQ=255)

                                                                                      aGGTACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                             <  2:1453810/150‑1 (MQ=255)

                                                                                      aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTATCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                             <  1:2938627/150‑1 (MQ=255)

                                                                                        gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                           <  2:3490759/150‑1 (MQ=255)

                                                                                           cacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTctct                                                        >  2:274021/1‑150 (MQ=255)

                                                                                              tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                     <  2:4296841/150‑1 (MQ=255)

                                                                                              tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                     <  2:1199938/150‑1 (MQ=255)

                                                                                               gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                    >  1:1915764/1‑150 (MQ=255)

                                                                                                  aGGTAAAATATTTTTTGGTTTTGTTGGTGATAATATTGGTGT‑AAAAAAAGACTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAtt                                                 <  1:3029952/150‑1 (MQ=255)

                                                                                                   ggTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                                <  2:2933684/150‑1 (MQ=255)

                                                                                                   ggTACAATATATTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                                >  2:3740868/1‑150 (MQ=255)

                                                                                                     tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                              >  1:222244/1‑150 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                          <  2:1365769/150‑1 (MQ=255)

                                                                                                                 tGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                  <  2:2886872/150‑1 (MQ=255)

                                                                                                                 tGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                  >  1:1752186/1‑150 (MQ=255)

                                                                                                                   gTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg                                <  2:3020859/150‑1 (MQ=255)

                                                                                                                       tgttgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAt                            >  2:3976771/1‑150 (MQ=255)

                                                                                                                       tgttgGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAt                            <  2:3327212/150‑1 (MQ=255)

                                                                                                                        gttgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                           <  1:749778/150‑1 (MQ=255)

                                                                                                                          tgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGc                         >  1:1237827/1‑150 (MQ=255)

                                                                                                                           ggTGATAAGATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTATTTGGATTATTGTTTACAAATTTAGTTATTGc                         >  2:4441188/1‑150 (MQ=255)

                                                                                                                           ggTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                        <  2:241233/150‑1 (MQ=255)

                                                                                                                            gTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                       <  1:1816063/150‑1 (MQ=255)

                                                                                                                             tGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGCTTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTCTTGCAgg                      >  2:2706485/1‑150 (MQ=255)

                                                                                                                             tGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                      <  1:129196/150‑1 (MQ=255)

                                                                                                                              gATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAggg                     >  1:2649932/1‑150 (MQ=255)

                                                                                                                               aTAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                    >  1:4224263/1‑150 (MQ=255)

                                                                                                                               aTAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTACTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATATGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTTCAGggt                    >  2:3555668/1‑150 (MQ=255)

                                                                                                                                tAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAGTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                   >  2:2096420/1‑150 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                  >  2:3262912/1‑150 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                  >  1:4316771/1‑150 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                  <  2:2535276/150‑1 (MQ=255)

                                                                                                                                   gATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt                <  1:4199378/150‑1 (MQ=255)

                                                                                                                                       ggTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa            >  2:2048564/1‑150 (MQ=255)

                                                                                                                                              aaaaaaACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAttt      >  1:934407/1‑150 (MQ=255)

                                                                                                                                                  aaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtatat  <  1:521398/150‑1 (MQ=255)

CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATAT  >  CP009273/4494574‑4494868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`