Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,531,143 A→T 100% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,531,1430AT95.5% 1020.0 / 27.9 311I124F (ATT→TTT) fimBtyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base A (5/9);  new base T (164/133);  total (169/142)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 321 total aligned reads displayed.

TTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAT  >  CP009273/4530996‑4531289
                                                                                                                                                   |                                                                                                                                                  
ttttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtttt                                                                                                                                                  >  2:4179125/1‑150 (MQ=255)
ttttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtttt                                                                                                                                                  >  2:583817/1‑150 (MQ=255)
  ttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAttttat                                                                                                                                                <  1:1343201/150‑1 (MQ=255)
     aacaacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTAtctc                                                                                                                                             >  1:3703299/1‑150 (MQ=255)
      acaacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGTGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCg                                                                                                                                            >  1:4445408/1‑150 (MQ=255)
      acaacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCg                                                                                                                                            >  1:3463706/1‑150 (MQ=255)
       caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                           >  2:2263692/1‑150 (MQ=255)
       caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                           <  1:2300369/150‑1 (MQ=255)
          cGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACtt                                                                                                                                        <  2:2016576/150‑1 (MQ=255)
          cGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACtt                                                                                                                                        <  2:2466396/150‑1 (MQ=255)
           gctcccGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCTTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTc                                                                                                                                       <  1:1502080/147‑1 (MQ=255)
                cGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtg                                                                                                                                  >  1:1849314/1‑150 (MQ=255)
                   tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTa                                                                                                                               >  2:3821853/1‑150 (MQ=255)
                   tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTa                                                                                                                               >  1:2708629/1‑150 (MQ=255)
                        aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                          >  1:1996701/1‑150 (MQ=255)
                         aTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCg                                                                                                                         >  1:1963886/1‑150 (MQ=255)
                               aagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGt                                                                                                                   <  2:1751791/150‑1 (MQ=255)
                               aagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGt                                                                                                                   >  2:2624814/1‑150 (MQ=255)
                                   tCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACt                                                                                                               >  2:420987/1‑150 (MQ=255)
                                     aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTgg                                                                                                             >  1:1917755/1‑150 (MQ=255)
                                     aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTgg                                                                                                             <  2:28369/150‑1 (MQ=255)
                                     aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTgg                                                                                                             >  2:3382982/1‑150 (MQ=255)
                                     aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTgg                                                                                                             >  2:1048621/1‑150 (MQ=255)
                                       gCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCGGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgag                                                                                                           >  1:4438684/1‑150 (MQ=255)
                                        cTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgaga                                                                                                          >  1:3839793/1‑150 (MQ=255)
                                         tttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGAt                                                                                                         >  2:1016101/1‑150 (MQ=255)
                                              aaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                    >  1:1673071/1‑150 (MQ=255)
                                              aaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                    >  1:4239757/1‑150 (MQ=255)
                                                aaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCg                                                                                                  >  2:3125559/1‑150 (MQ=255)
                                                    ggTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGcaca                                                                                              <  2:4271021/150‑1 (MQ=255)
                                                     gTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACAt                                                                                             <  1:2902612/150‑1 (MQ=255)
                                                       tGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGt                                                                                           >  2:1184567/1‑150 (MQ=255)
                                                       tGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGt                                                                                           >  1:4229709/1‑150 (MQ=255)
                                                       tGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGt                                                                                           <  2:890276/150‑1 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         <  1:583817/150‑1 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         >  2:1878879/1‑150 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         <  1:4178201/150‑1 (MQ=255)
                                                         aGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                         >  2:871711/1‑150 (MQ=255)
                                                          gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                        <  1:235629/150‑1 (MQ=255)
                                                          gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                        <  2:4494210/150‑1 (MQ=255)
                                                           tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACg                                                                                       <  1:3655129/150‑1 (MQ=255)
                                                            aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                      >  1:678324/1‑150 (MQ=255)
                                                            aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                      >  2:2618703/1‑150 (MQ=255)
                                                            aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                      >  2:2434865/1‑150 (MQ=255)
                                                                 tACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTGTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTc                                                                                 <  2:2965583/150‑1 (MQ=255)
                                                                      cGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGtgtg                                                                            >  1:2798947/1‑150 (MQ=255)
                                                                        tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGt                                                                          >  2:952741/1‑150 (MQ=255)
                                                                          cccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                        <  2:313685/150‑1 (MQ=255)
                                                                           ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGtttt                                                                       <  2:4011917/150‑1 (MQ=255)
                                                                               aTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                   >  2:4304875/1‑150 (MQ=255)
                                                                               aTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                   >  2:2126340/1‑150 (MQ=255)
                                                                                  cTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTgg                                                                >  2:4128090/1‑150 (MQ=255)
                                                                                   tGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                               >  1:2230434/1‑150 (MQ=255)
                                                                                     agagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGa                                                             <  2:3208664/150‑1 (MQ=255)
                                                                                              gggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGaa                                                    >  2:4310616/1‑150 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   <  1:3280824/150‑1 (MQ=255)
                                                                                               ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                   <  1:873037/150‑1 (MQ=255)
                                                                                                gTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATa                                                  <  2:1367688/150‑1 (MQ=255)
                                                                                                 tATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAg                                                 <  2:2722270/150‑1 (MQ=255)
                                                                                                 tATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAg                                                 <  1:4097426/150‑1 (MQ=255)
                                                                                                   tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGAt                                               <  1:2485773/150‑1 (MQ=255)
                                                                                                      ttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcg                                            <  2:2878123/150‑1 (MQ=255)
                                                                                                      ttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcg                                            <  2:1121930/150‑1 (MQ=255)
                                                                                                           aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACt                                       <  2:1552505/150‑1 (MQ=255)
                                                                                                           aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACt                                       <  2:1551975/150‑1 (MQ=255)
                                                                                                             gTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGACTATGGGAATAGATACGCGACTTa                                     >  2:3238956/1‑150 (MQ=255)
                                                                                                              tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                    <  2:283885/150‑1 (MQ=255)
                                                                                                              tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                    <  1:4280412/150‑1 (MQ=255)
                                                                                                               taGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCAGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                   <  1:2909501/149‑1 (MQ=255)
                                                                                                               aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                   <  2:2213040/150‑1 (MQ=255)
                                                                                                               aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                   <  2:3463706/150‑1 (MQ=255)
                                                                                                               aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                   <  1:3382982/150‑1 (MQ=255)
                                                                                                                aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATcc                                  <  2:3776778/150‑1 (MQ=255)
                                                                                                                aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATcc                                  <  2:1716947/150‑1 (MQ=255)
                                                                                                                 ggggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCa                                 <  1:534065/150‑1 (MQ=255)
                                                                                                                  gggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAg                                <  1:3797466/150‑1 (MQ=255)
                                                                                                                   ggAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAgg                               >  2:3931898/1‑150 (MQ=255)
                                                                                                                    gAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                              <  1:4528022/150‑1 (MQ=255)
                                                                                                                    gAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                              <  1:1679401/150‑1 (MQ=255)
                                                                                                                      aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                            >  1:1257886/1‑150 (MQ=255)
                                                                                                                          gCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCt                        <  1:1125498/150‑1 (MQ=255)
                                                                                                                            tttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACCTGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                      >  2:766354/1‑150 (MQ=255)
                                                                                                                            tttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                      <  2:2072483/150‑1 (MQ=255)
                                                                                                                            tttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                      <  2:2071057/150‑1 (MQ=255)
                                                                                                                              tctcGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg                    <  2:3338127/150‑1 (MQ=255)
                                                                                                                              tctcGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg                    <  2:1063725/150‑1 (MQ=255)
                                                                                                                               ctcGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGc                   <  1:1325994/150‑1 (MQ=255)
                                                                                                                                   gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCg               <  2:3731410/150‑1 (MQ=255)
                                                                                                                                   gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCg               >  2:2326514/1‑150 (MQ=255)
                                                                                                                                    cAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGc              >  2:325255/1‑150 (MQ=255)
                                                                                                                                       cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat           >  1:1023582/1‑150 (MQ=255)
                                                                                                                                       cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat           <  1:3209212/150‑1 (MQ=255)
                                                                                                                                       cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat           >  1:403360/1‑150 (MQ=255)
                                                                                                                                        aGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata          >  2:3779484/1‑150 (MQ=255)
                                                                                                                                        aGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTGTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata          >  2:2294606/1‑150 (MQ=255)
                                                                                                                                         gTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGTTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAatat         <  2:2991601/150‑1 (MQ=255)
                                                                                                                                           tttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTc       >  2:4166404/1‑150 (MQ=255)
                                                                                                                                            ttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCg      <  2:3641301/150‑1 (MQ=255)
                                                                                                                                               ccATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCa   <  1:3228984/150‑1 (MQ=255)
                                                                                                                                                cATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAt  <  1:3720909/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCAT  >  CP009273/4530996‑4531289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑