BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,572,227	G→T	43.0%	I213I (ATC→ATA)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,572,227	0	G	T	43.0%	211.2 / 270.4	223	I213I (ATC→ATA)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (44/83); new base T (56/40); total (100/123)
Fisher's exact test for biased strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

ACAATATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTT  >  CP009273/4572078‑4572376

aCAATATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCg                                                                                                                                                       >  2:1070604/1‑150 (MQ=255)

  aaTATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGAt                                                                                                                                                     <  1:1275135/150‑1 (MQ=255)

  aaTATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGAt                                                                                                                                                     <  1:1275625/150‑1 (MQ=255)

  aaTATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGAt                                                                                                                                                     >  2:3045056/1‑150 (MQ=255)

  aaTATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGAt                                                                                                                                                     >  1:483601/1‑150 (MQ=255)

     atGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATaaa                                                                                                                                                  >  2:3756413/1‑150 (MQ=255)

       gcgcCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcg                                                                                                                                                <  2:1125419/150‑1 (MQ=255)

         gcCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcgcg                                                                                                                                              <  2:2520720/150‑1 (MQ=255)

         gcCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcgcg                                                                                                                                              >  2:139199/1‑150 (MQ=255)

            ttCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCgg                                                                                                                                           >  2:3139793/1‑150 (MQ=255)

             tCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGt                                                                                                                                          >  2:2244664/1‑150 (MQ=255)

              cGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTg                                                                                                                                         <  2:103817/150‑1 (MQ=255)

               ggCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTgg                                                                                                                                        >  2:307112/1‑150 (MQ=255)

               ggCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTgg                                                                                                                                        >  1:4400027/1‑150 (MQ=255)

                  aGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATc                                                                                                                                     <  1:735805/150‑1 (MQ=255)

                  aGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATc                                                                                                                                     >  2:2486536/1‑150 (MQ=255)

                         cACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAAt                                                                                                                              >  1:4051936/1‑150 (MQ=255)

                         cACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAAt                                                                                                                              <  1:894183/150‑1 (MQ=255)

                         cACCGTCGCTACCCACAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAAt                                                                                                                              >  1:1770107/1‑150 (MQ=255)

                            cGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCt                                                                                                                           <  1:701265/150‑1 (MQ=255)

                            cGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCt                                                                                                                           >  2:3563405/1‑150 (MQ=255)

                              tCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGc                                                                                                                         >  2:2399087/1‑150 (MQ=255)

                               cGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCg                                                                                                                        <  1:494647/150‑1 (MQ=255)

                                gCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCgt                                                                                                                       <  1:4224638/150‑1 (MQ=255)

                                   aCCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTc                                                                                                                    >  1:3313690/1‑150 (MQ=255)

                                   aCCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTc                                                                                                                    >  1:3631515/1‑150 (MQ=255)

                                   aCCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTc                                                                                                                    <  2:25919/150‑1 (MQ=255)

                                       agagTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCa                                                                                                                >  1:2187649/1‑150 (MQ=255)

                                           tgtTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCaa                                                                                                            <  1:4492697/150‑1 (MQ=255)

                                                  cAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgtcgtc                                                                                                     <  1:1645642/150‑1 (MQ=255)

                                                   aGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTcc                                                                                                    <  1:413725/150‑1 (MQ=255)

                                                   aGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTcc                                                                                                    <  1:1135046/150‑1 (MQ=255)

                                                      cGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGa                                                                                                 <  2:4460344/150‑1 (MQ=255)

                                                         aTTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCa                                                                                              >  2:232292/1‑150 (MQ=255)

                                                         aTTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCa                                                                                              >  2:228818/1‑150 (MQ=255)

                                                         aTTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCa                                                                                              >  2:518819/1‑150 (MQ=255)

                                                          ttGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCAt                                                                                             <  1:27057/150‑1 (MQ=255)

                                                           tGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCAtt                                                                                            >  2:2687918/1‑150 (MQ=255)

                                                           tGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCAtt                                                                                            <  2:1379062/150‑1 (MQ=255)

                                                            gcgcCGCCGTTGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTg                                                                                           <  1:4317734/150‑1 (MQ=255)

                                                               ccgccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGtt                                                                                        <  2:683474/150‑1 (MQ=255)

                                                                  ccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGc                                                                                     <  1:1063952/150‑1 (MQ=255)

                                                                  ccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGc                                                                                     <  1:1063817/150‑1 (MQ=255)

                                                                   cgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCg                                                                                    <  2:2082497/150‑1 (MQ=255)

                                                                   cgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCg                                                                                    <  2:717415/150‑1 (MQ=255)

                                                                   cgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCg                                                                                    <  1:831627/150‑1 (MQ=255)

                                                                   cgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCg                                                                                    >  2:1845115/1‑150 (MQ=255)

                                                                       gTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACtt                                                                                <  1:253212/150‑1 (MQ=255)

                                                                        tCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTa                                                                               <  2:1514911/150‑1 (MQ=255)

                                                                          gAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAAc                                                                             <  2:1062341/150‑1 (MQ=255)

                                                                           aGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACa                                                                            >  2:609810/1‑150 (MQ=255)

                                                                            ggTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACAt                                                                           <  1:3845647/150‑1 (MQ=255)

                                                                            ggTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACAt                                                                           <  2:4337436/150‑1 (MQ=255)

                                                                               tGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGc                                                                        >  2:1506091/1‑150 (MQ=255)

                                                                               tGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGc                                                                        <  1:3151526/150‑1 (MQ=255)

                                                                               tGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGc                                                                        <  1:3151072/150‑1 (MQ=255)

                                                                                gCCTTCAATATCGTGCGGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCg                                                                       <  1:887626/150‑1 (MQ=255)

                                                                                gCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCg                                                                       <  1:2987052/150‑1 (MQ=255)

                                                                                  cTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGACATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGt                                                                     >  2:4027709/1‑150 (MQ=255)

                                                                                  cTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTAGCGACTTAACATAGCGGt                                                                     >  2:63634/1‑150 (MQ=255)

                                                                                   ttCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACTCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTc                                                                    >  2:779132/1‑150 (MQ=255)

                                                                                   ttCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTc                                                                    >  2:4342434/1‑150 (MQ=255)

                                                                                    tCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAATCGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCg                                                                   <  1:3702181/150‑1 (MQ=255)

                                                                                        tatCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCtt                                                               <  1:321523/150‑1 (MQ=255)

                                                                                        tatCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCtt                                                               <  1:2973010/150‑1 (MQ=255)

                                                                                             tGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTtcaatc                                                          >  2:3570204/1‑150 (MQ=255)

                                                                                              gcagcaGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTtcaatca                                                         <  1:2888498/150‑1 (MQ=255)

                                                                                                   aggcagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAg                                                    <  1:4098411/150‑1 (MQ=255)

                                                                                                   aggcagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAg                                                    <  1:39655/150‑1 (MQ=255)

                                                                                                    ggcagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGc                                                   >  1:1416301/1‑150 (MQ=255)

                                                                                                       agTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccg                                                <  1:4292204/150‑1 (MQ=255)

                                                                                                       agTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccg                                                >  2:1952928/1‑150 (MQ=255)

                                                                                                            atcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAACTCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTa                                           >  2:4193583/1‑150 (MQ=255)

                                                                                                            atcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTa                                           >  2:4193516/1‑150 (MQ=255)

                                                                                                                gTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTg                                       <  2:1804838/150‑1 (MQ=255)

                                                                                                                gTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTg                                       <  1:188163/150‑1 (MQ=255)

                                                                                                                     aGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTgccgcc                                  >  2:1988984/1‑150 (MQ=255)

                                                                                                                     aGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTgccgcc                                  >  2:158394/1‑150 (MQ=255)

                                                                                                                     aGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTgccgct                                  <  1:2664191/150‑2 (MQ=255)

                                                                                                                            gggTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCt                           <  1:2750090/150‑1 (MQ=255)

                                                                                                                                   gggCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcaccac                    >  1:1864538/1‑150 (MQ=255)

                                                                                                                                   gggCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcaccac                    >  2:3363397/1‑150 (MQ=255)

                                                                                                                                   gggCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcaccac                    >  2:3303031/1‑150 (MQ=255)

                                                                                                                                    ggCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACt                   >  1:1684363/1‑150 (MQ=255)

                                                                                                                                    ggCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACt                   <  2:271883/150‑1 (MQ=255)

                                                                                                                                    ggCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACt                   >  1:358876/1‑150 (MQ=255)

                                                                                                                                      cACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTc                 <  1:1294335/150‑1 (MQ=255)

                                                                                                                                      cACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTc                 <  1:1864886/150‑1 (MQ=255)

                                                                                                                                         cAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcg              >  2:322506/1‑150 (MQ=255)

                                                                                                                                         cAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcg              <  1:210459/150‑1 (MQ=255)

                                                                                                                                          aGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcgc             >  2:2208092/1‑150 (MQ=255)

                                                                                                                                           gTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcgcg            <  1:1070608/150‑1 (MQ=255)

                                                                                                                                             tCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGGTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGc          >  2:2953537/1‑150 (MQ=255)

                                                                                                                                                aGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGc       >  2:4220304/1‑150 (MQ=255)

                                                                                                                                                 ggCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCg      <  1:3282443/150‑1 (MQ=255)

                                                                                                                                                 ggCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCg      <  1:2803152/150‑1 (MQ=255)

                                                                                                                                                 ggCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCg      >  2:1805632/1‑150 (MQ=255)

                                                                                                                                                   ccGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGt    >  2:3226653/1‑150 (MQ=255)

                                                                                                                                                     gATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGttt  >  1:719268/1‑150 (MQ=255)

                                                                                                                                                     gATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGttt  >  2:1624041/1‑150 (MQ=255)

ACAATATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTT  >  CP009273/4572078‑4572376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`